The Citrus Flavone Nobiletin Reduces Pro-Inflammatory and Pro-Labour Mediators in Fetal Membranes and Myometrium: Implications for Preterm Birth
Figure 2
Effect of nobiletin on LPS-induced cytokine expression and release in term fetal membranes.
Fetal membranes were incubated with or without 10 µg/mL of LPS in the absence or presence 200 µM of nobiletin for 20 h (n = 6 patients per group). (A–D) TNF-α, IL-1β, IL-6 and IL-8 mRNA expression was analysed by qRT-PCR and normalised to GAPDH mRNA expression. The relative fold change was calculated relative to LPS and data presented as mean ± SEM. *P<0.05 vs. LPS (one-way ANOVA). (E–H) The incubation medium was assayed for concentration of TNF-α, IL-1β, IL-6 and IL-8 by enzyme immunoassay. Each bar represents mean concentration ± SEM. *P<0.05 vs. LPS (one-way ANOVA).
