Changes in Acetyl CoA Levels during the Early Embryonic Development of Xenopus laevis
Figure 5
Whole-embryo acetylation levels of core histones during early Xenopus embryonic development.
20 embryos collected at different stages of development were lysed in 80 µl lysis buffer and approximately 40 µg of soluble protein was separated by SDS-PAGE, transferred to PVDF membranes and probed with either site-specific anti-acetyl histone antibodies or total anti-histone antibodies. Total and acetylated histones were determined on separate membranes. Representative blots are shown. The graphs show means +/− SEM of normalised band intensity/size obtained from 3 independent fertilisations. The band intensity/size of total and acetylated histones at each stage is expressed relative to the mean of all the stages analysed.
