Mouse Model of Lymph Node Metastasis via Afferent Lymphatic Vessels for Development of Imaging Modalities
Figure 3
Parameters related to the incidence of metastasis.
A. Effects of inoculation conditions on the incidence of lymph node metastasis. Metastatic incidence increased with injection of a larger number cells (a), did not vary significantly with injection duration (b), but decreased with a larger SiLN volume (c). In the values presented above the bars, the denominator represents the number of inoculated mice while the numerator represents the number of mice with metastases in the proper-ALN. NS indicates P>0.05; *P<0.05 calculated by Fisher’s exact probability test. B. Assessment of the correlation between the above 3 parameters and metastasis incidence, using a new parameter, cells mm−3 min−1. When metastasis incidence was set at 1, 100% metastasis was achieved when the cells mm−3 min−1 value exceeded 4.72×102 (n = 39). Analyses were performed with the Mann-Whitney U test.
