A Predictive Model of Intein Insertion Site for Use in the Engineering of Molecular Switches
Figure 6
Xylanase diagnostic plate phenotypes of intein modified XynB (XynB:Tth) constructs.
The T. thermophilus DnaE-1 intein (Tth) was inserted into 20 sites of XynB and expressed from lamda phage. λXynB:Tth infected E.coli were grown on xylanase diagnostic plates containing the insoluble substrate AZCL-xylane embedded in the top agarose. Xylanase activity was scored following heat treatment of the confluent lysis plates at 60°C/3 hr. Blue color development indicates xylanase activity. The plates shown are representative of the three different activity groups: active, partially active and inactive.
