Evaluation of Pulsed-FRAP and Conventional-FRAP for Determination of Protein Mobility in Prokaryotic Cells
Figure 3
Comparison of pulsed-FRAP with “conventional FRAP”.
Diffusion coefficients of GFP in the cytoplasm of E. coli measured with “conventional FRAP” (gray bars) and pulsed-FRAP (black bars) under normal osmotic conditions (top panel, “+ 0 mM NaCl”) and under conditions of osmotic upshift (lower panels, the extent of osmotic shock is indicated in the upper right corner of each panel). For the highest osmotic stress (lowest panel, “+500 mM NaCl”) only “conventional FRAP” data are presented. The number of cells measured in each experiment was at least 20.
