Akt Deficiency Attenuates Muscle Size and Function but Not the Response to ActRIIB Inhibition
Figure 4
Effects of Akt deficiency and ActRIIB inhibition on EDL fiber size and distribution.
(A) Effects of genotype and ActRIIB-mFc treatment on EDL fiber cross-sectional area distributions. Wild-type (WT, red), Akt1 knockout mice (Akt1 KO, green), Akt2 knockout mice (Akt2 KO, blue), ActRIIB-mFc -treated WT (WT Rx, red-dashed), ActRIIB-mFc -treated Akt1 KO (Akt1 KO Rx, green dashed), and ActRIIB-mFc -treated Akt2 KO (Akt2 KO Rx, blue dashed) EDL fiber distributions; n≥792 fibers per group; (B) Single fiber cross sectional areas per genotype (Veh, white bar) and treatment (Rx, black bar); (C) Number of fibers in the EDL. Data are mean +/− SEM, n = 5; +++P<0.0001 vs. WT treated with vehicle; ***P<0.0001 vs. same genotype treated with vehicle.
