The Hmr and Lhr Hybrid Incompatibility Genes Suppress a Broad Range of Heterochromatic Repeats
Figure 8
Analysis of Lhr function in D. simulans.
(A) Immuno-FISH experiment shows that the brightest mel-Lhr foci colocalize with dodeca (red, arrow) and GA satellites (white, arrowhead) in D. melanogaster (upper panel). The brightest sim-Lhr foci either colocalize or are juxtaposed with dodeca (arrow) but are not associated with GA-rich satellites (arrowhead). All panels contain DAPI shown in blue. Scale bar = 10 µm. (B) Fold changes in TE expression between w501; Lhr1 and w501; Lhr+ were calculated for uniquely mapping reads with zero mismatches to the individual-insertion database and with three mismatches to the consensus-sequence database. Three mismatches are required to account for the divergence of TE insertions in D. simulans from the consensus sequences, which are largely defined from D. melanogaster TEs. The 25 most significantly derepressed TE families in the individual-insertion sequence based analysis are shown here (excluding centroids), as well as TAHRE, which is found only in the consensus-sequence database. Classification of DNA, LTR and non-LTR elements is from reference [99]. (C) Comparison of TE misregulation between D. melanogaster and D. simulans Lhr mutations. The diagram includes all TE families that were upregulated at least two fold, including those in individual-insertion database analysis as well as those that are only represented in the consensus-sequence database analysis. (D) Comparison of euchromatic and heterochromatic gene expression in D. simulans w501; Lhr1, as described in Figure 4. The euchromatin-heterochromatin border has not been experimentally determined in D. simulans and was defined from D. melanogaster, Analysis includes 7479 euchromatic and 350 heterochromatic genes (p = 0.12, Wilcoxon rank sum test with continuity correction).
