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Correction: G-Protein-Coupled Estrogen Receptor Agonist Suppresses Airway Inflammation in a Mouse Model of Asthma through IL-10

  • Masamichi Itoga,
  • Yasunori Konno,
  • Yuki Moritoki,
  • Yukiko Saito,
  • Wataru Ito,
  • Mami Tamaki,
  • Yoshiki Kobayashi,
  • Hiroyuki Kayaba,
  • Yuta Kikuchi,
  • Junichi Chihara,
  • Masahide Takeda,
  • Shigeharu Ueki,
  • Makoto Hirokawa

Correction: G-Protein-Coupled Estrogen Receptor Agonist Suppresses Airway Inflammation in a Mouse Model of Asthma through IL-10

  • Masamichi Itoga, 
  • Yasunori Konno, 
  • Yuki Moritoki, 
  • Yukiko Saito, 
  • Wataru Ito, 
  • Mami Tamaki, 
  • Yoshiki Kobayashi, 
  • Hiroyuki Kayaba, 
  • Yuta Kikuchi, 
  • Junichi Chihara
PLOS
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In the Materials and Methods section, there is an error in the fifth and sixth sentences of the section titled “Flow cytometry.” This sentence should read: 2), PE-Cy5-conjugated anti-TCRβ (BioLegend, San Diego, CA), PE-Cy7-conjugated ant-NK1.1 (BioLegend), PE-Cy7-conjugated anti-CD8a (BioLegend), PE-CF594-conjugated anti-CD4 (BD Biosciences), and GPR30 (N-15)-R Antibody (Santa Cruz Biotechnology, Santa Cruz, CA) with subsequent staining by Alexa Fluor 488-conjugated goat anti-rabbit immunoglobulin G (IgG) (Invitrogen, Grand Island, NY). Then, the cells were washed, permeabilized with BD Cytofix/Cytoperm (BD Biosciences), and stained with PE-conjugated anti-IL-10 (BioLegend) to detect intracellular IL-10.

Reference

  1. 1. Itoga M, Konno Y, Moritoki Y, Saito Y, Ito W, Tamaki M, et al. (2015) G-Protein-Coupled Estrogen Receptor Agonist Suppresses Airway Inflammation in a Mouse Model of Asthma through IL-10. PLoS ONE 10(3): e0123210. doi:10.1371/journal.pone.0123210. pmid:25826377