In the Materials and Methods section, there is an error in the fifth and sixth sentences of the section titled “Flow cytometry.” This sentence should read: 2), PE-Cy5-conjugated anti-TCRβ (BioLegend, San Diego, CA), PE-Cy7-conjugated ant-NK1.1 (BioLegend), PE-Cy7-conjugated anti-CD8a (BioLegend), PE-CF594-conjugated anti-CD4 (BD Biosciences), and GPR30 (N-15)-R Antibody (Santa Cruz Biotechnology, Santa Cruz, CA) with subsequent staining by Alexa Fluor 488-conjugated goat anti-rabbit immunoglobulin G (IgG) (Invitrogen, Grand Island, NY). Then, the cells were washed, permeabilized with BD Cytofix/Cytoperm (BD Biosciences), and stained with PE-conjugated anti-IL-10 (BioLegend) to detect intracellular IL-10.
Reference
Citation: Itoga M, Konno Y, Moritoki Y, Saito Y, Ito W, Tamaki M, et al. (2015) Correction: G-Protein-Coupled Estrogen Receptor Agonist Suppresses Airway Inflammation in a Mouse Model of Asthma through IL-10. PLoS ONE 10(8): e0136326. https://doi.org/10.1371/journal.pone.0136326
Published: August 14, 2015
Copyright: © 2015 Itoga et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited