CRISPR/Cas9 -mediated gene knockout of Anopheles gambiae FREP1 suppresses malaria parasite infection
Dimopoulos and colleagues establish a modified CRISPR/Cas9 genome editing technique for malaria vector A. gambiae mosquitoes. Through this approach, they inactivate the fibrinogen-related protein 1 (FREP1) gene, via CRISPR/Cas9 genome editing, and the impact of this manipulation on the mosquito’s susceptibility to Plasmodium and on mosquito fitness.
Image credit: Dong Y, et al. (2018)