Figures
The captions for Fig 1 through Fig 6 are missing. Please see the complete captions here:
○: by standard strategy initiating induction at high cells concentration with pure oxygen aeration at 14 h and implementing the modified glycerol feeding strategy at about 22 h (A). ●: initiating induction at low cells concentration with air aeration/standard DO-Stat feeding (C).
(A)-(D): initiating methanol induction at high cell concentration; (E)-(H): initiating induction at low cell concentration. ●, ■ and □: induction at 30°C; ○, □ and ┄: at 20 °C; ▲ and —: at 30 °C but aerating pure oxygen.
CY: methanol distribution ratio for cells growth and maintenance; CP: methanol distribution ratio for protein synthesis; CE: methanol distribution ratio for NADH (energy) formation. ηH: the ratio of data categorized into high energy utilization efficiency η (η>0.8). 1, 2, 3 and 6: referred to fermentation runs numbers.
(A): specific cells growth rates (μ); (B): specific methanol consumption rates (v); (C): specific monellin production rates (ρ); (D): specific methanol consumption rates (v) versus specific cells growth rates (μ); (E): specific monellin production rates (ρ) versus specific cells growth rates (μ). ● and □: induction at low cells concentration and 30°C (run #3); ○ and —: at low cells concentration and 20°C (run # 6); ■ and …: at high cells concentration and 30°C (run #1); □ and ┄: at high cells concentration and 20°C (run #2).
(A): Categories of NADH distribution η (rCNADH/rFNADH) under different induction strategies (runs #1–3, #6). █: η≥1.0; ░: 0.8<η<1.0; □: 0.0≤η≤0.8. (B): NADH distribution η (rCNADH/rFNADH) versus induction time under the two extreme induction strategies (run #1 and #3). □: run #1; ●: run #3. (C): specific rFNADH versus specific growth rate (μ) when initiating induction at low cell concentration while maintaining induction temperature at 30°C/20°C. ●: run #3, ○: run #6. (D): NADH distribution η (rCNADH/rFNADH) versus specific growth rate (μ) when initiating induction at low cell concentration while maintaining induction temperature at 30°C/20°C. ●: run #3, ○: run #6.
○: run#1; ●: run#3.
Reference
- 1. Jia L, Tu T, Huai Q, Sun J, Chen S, Li X, et al. (2017) Enhancing monellin production by Pichia pastoris at low cell induction concentration via effectively regulating methanol metabolism patterns and energy utilization efficiency. PLoS ONE 12(10): e0184602. https://doi.org/10.1371/journal.pone.0184602 pmid:28981536
Citation: Jia L, Tu T, Huai Q, Sun J, Chen S, Li X, et al. (2018) Correction: Enhancing monellin production by Pichia pastoris at low cell induction concentration via effectively regulating methanol metabolism patterns and energy utilization efficiency. PLoS ONE 13(7): e0201085. https://doi.org/10.1371/journal.pone.0201085
Published: July 18, 2018
Copyright: © 2018 Jia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.