Effects of polysaccharide extracts from Ganoderma lucidum and Amauroderma rude on breast cancer cell death.
The life cycle of Amauroderma rude contains spore, mycelia, and fruit body (a). Cells of MT-1 (b), MDA-MB231 (c), 4T1 (d), MDA-MB468 (e), and MCF-7 (f) were inoculated in 24-well tissue culture plates at a density of the 8×104 cells/well. Four hours after cell inoculation, extracts from Ganoderma lucidum and Amauroderma rude were added to each well at different concentrations (0, 50, 100, 200, 400, 600, 800 and 1000 µg/ml) and incubated for 48 hours. Cell survival was determined by trypan blue staining and cell counting. n = 3, *p<0.05; **p<0.01.
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