Variations in Glycogen Synthesis in Human Pluripotent Stem Cells with Altered Pluripotent States
(A) Schema of the glycogen assay based on the protocol from BioVision Inc.: glycogen was hydrolyzed (H) into glucose, then glucose developed (D) into an intermediate and reduce probe (P) to produce color products. (B) Determination of glycogen content in hPSCs grown under various growth conditions. Column 1: MCF7 breast cancer cells used control (Cont) for the assessment of glycogen levels; Column 2: H1 colonies grown on MEF as previously descried in Materials and Methods. The glycogen content was measured at cell passage number 38. Column 3: H1 colonies initially grown on MEF for 35 passages followed by growing on 2.5% BD Matrigel (MG) in mTeSR1 for 5 passages. Column 4: H9 cells were initially grown on MEF for 32 passages followed by passaging on 2.5% BD Matrigel (MG) in mTeSR1 medium for 9 passages. Column 5: BC1 human iPSCs were initially grown on MEF for 50 passages followed by passaging on 2.5% BD Matrigel (MG) in mTeSR1 medium for 26 passages.