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Functional Conservation of Cis-Regulatory Elements of Heat-Shock Genes over Long Evolutionary Distances

Figure 1

Induction of promoters of Drosophila and human heat-shock genes in C. elegans.

A) Worms carrying reporter transgenes were heat shocked at 33°C for the indicated periods of time. The mRNA level was measured by quantitative RT-PCR. Each data point represents 3 independent biological replicates (with standard error). B) Same as in A), except each data point represents 2 independent biological replicates. C) Worms carrying reporter transgenes were heat shocked at 35°C, and 30–40 minutes later the mRNA level were measured by quantitative RT-PCR in 2 independent biological replicates.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0022677.g001