Functional Conservation of Cis-Regulatory Elements of Heat-Shock Genes over Long Evolutionary Distances
A) Worms carrying reporter transgenes were heat shocked at 33°C for the indicated periods of time. The mRNA level was measured by quantitative RT-PCR. Each data point represents 3 independent biological replicates (with standard error). B) Same as in A), except each data point represents 2 independent biological replicates. C) Worms carrying reporter transgenes were heat shocked at 35°C, and 30–40 minutes later the mRNA level were measured by quantitative RT-PCR in 2 independent biological replicates.