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A High-Throughput Screen for Tuberculosis Progression

Figure 1

Outcome of M. marinum yolk sac injection of embryos between the 16- and the 512-cell stage.

(A) 5 dpi fli1-egfp larva with gfp-labelled vasculature showing spread of bacteria (red) throughout the body (scale bar: 250 µm). (B and D) Bright-field/fluorescence overlay and (C and E) confocal z-stack of red-fluorescent bacteria showing activation of green-fluorescent gags at the (B and C) edge of the yolk extension and on the (D and E) tail of a 7 day-old larva (scale bar: 25 µm). (F and H) Bright-field confocal plane and (G and I) confocal z-stack of red-fluorescent bacteria co-localizing with green-fluorescent leukocytes detected by L-plastin immunostaining (scale bar: 25 µm). The lesions caused by the granulomas can be clearly seen in F and H.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0016779.g001