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closeWhy a standard curve is always essential!
Posted by ric_ataide on 03 Jun 2014 at 00:17 GMT
Today this assay can be performed at an even higher throughput through the use of 'dispensing robots' which greatly reduce the time spent pipetting solutions into the wells, which provides a fantastic opportunity to evaluate more serum samples with the same batch of THP-1 cells.
Although THP-1 cells do not tend to vary much in their behaviour (see this attached image from my thesis <a href="http://imgur.com/fXMf7Pf"><img src="http://i.imgur.com/fXMf7P..." title="Hosted by imgur.com"/></a> ), there are some days when you can get a reduced phagocytic activity due to external factors (different temperatures, different timings, etc.). This is why it is absolutely essential, when evaluating large cohorts that will be run on different days in different assays, to have standard curves and not just a positive and negative control. I am sharing a very simple image where this can be better visualised. Although the image represents extreme cases, it should highlight the importance of NOT conducting an assay without the standard curve.
<a href="http://imgur.com/fpFtD0f"><img src="http://i.imgur.com/fpFtD0..." title="Hosted by imgur.com"/></a>
