Here we present evidence of phytoliths preserved in carbonised food deposits on prehistoric pottery from the western Baltic dating from 6,100 cal BP to 5750 cal BP. Based on comparisons to over 120 European and Asian species, our observations are consistent with phytolith morphologies observed in modern garlic mustard seed (Alliaria petiolata (M. Bieb) Cavara & Grande). As this seed has a strong flavour, little nutritional value, and the phytoliths are found in pots along with terrestrial and marine animal residues, these findings are the first direct evidence for the spicing of food in European prehistoric cuisine. Our evidence suggests a much greater antiquity to the spicing of foods than is evident from the macrofossil record, and challenges the view that plants were exploited by hunter-gatherers and early agriculturalists solely for energy requirements, rather than taste.
Citation: Saul H, Madella M, Fischer A, Glykou A, Hartz S, Craig OE (2013) Phytoliths in Pottery Reveal the Use of Spice in European Prehistoric Cuisine. PLoS ONE 8(8): e70583. https://doi.org/10.1371/journal.pone.0070583
Editor: Janet M. Monge, University of Pennsylvania, United States of America
Received: February 13, 2013; Accepted: June 20, 2013; Published: August 21, 2013
Copyright: © 2013 Saul et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The Baltic Forgaers and Early Farmers Ceramic Research Project is an Arts and Humanities Research Council (AHRC) funded project (AH/E008232/1). The AHRC website can be found at http://www.ahrc.ac.uk/Pages/Home.aspx. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
It has been plausibly argued that two of the most important events in world history were the nearly simultaneous voyages to America by Columbus and around Africa to India by Vasco da Gama . Both these explorations were driven by a European desire for spice, documented in written sources from the classical period . Such efforts culminated in the economic ethic of free-enterprise, colonialism and ultimately capitalism . But is this taste for spice older? Classical texts testify to the widespread use of spices in European cuisine as far back in time as the 5th millennium BP –. In addition, archaeological studies of plant macrofossils have suggested that nutritionally poor but aromatically potent plants were available, and possibly used in cooking, in Neolithic Europe. The occasional preservation of seeds and peridermal tissues of plants, such as the opium poppy (Papaver somniferum L.), and aromatic herbs such as dill (Anethum graveolens L.), show that these spices spread from the Eastern Mediterranean, where their wild progenitors are found, to the Atlantic coastal margins c. 5,000 cal BP [8–17, Figure 1]. Earlier prehistoric evidence for the use of native European spices has been hard to demonstrate since seasonings originating from softer plant tissue can be invisible in charred fractions (e.g. leaves such as parsley Petroselinum crispum (Mill.) Fuss), or possible contenders are naturally abundant in the wild floral assemblages of excavated sediments. The current evidence is usually taken as support that Early Neolithic and pre-Neolithic uses of plants, and the reasons for their cultivation, were primarily driven by energy requirements rather than flavour .
Showing, A) A map of Europe showing an inset of the study area and sites from which the pot residues were acquired;, including also the Near East and northern Africa indicating early contexts where spices have been recovered: a) Menneville, France (Papaver somniferum L.), b) Eberdingen, Germany (Papaver somniferum L.), c) Seeberg, Switzerland (Papaver somniferum L.), d) Niederwil, Switzerland (Papaver somniferum L.), e) Swiss Lake Villages, Switzerland (Anethum graveolens L.), f) Cueva de los Murcielags, Spain (Papaver somniferum L.), g) Hacilar, Turkey (Capparis spinosa L.), h) Tell Abu Hureya, Syria (Caparis spinosa L.), i) Tell ed-Der, Syria (Coriandrum sativum L. and Cuminum cyminum L.), j) Khafaji, Iraq (Cruciferae family), k) Tell Aswad, Syria (Capparis spinosa L.), l) Nahal Hemar Cave, Israel (Coriandrum sativum L.), m) Tutankhamun's tomb, Egypt (Coriandrum sativum L.), n) Tomb of Kha, Egypt (Cuminum cyminum L.), o) Tomb of Amenophis II, Egypt (Anethum graveolens L.), p) Hala Sultan Tekke, Cyprus (Capparis spinosa L.), q) Heilbronn, Germany (Papaver somniferum L.), r) Zeslawice, Poland (Papaver somniferum L.) [compiled using 8–17]. B) Hunter-gatherer pointed-based vessel (on the left) and Early Neolithic flat-based vessel (on the right). C) Scanning Electron Microscope image of a globular sinuate phytolith embedded in a food residue, D) optical light microscope image of modern Alliaria petiolata globular sinuate phytoliths, and E) optical light microscope image of archaeological globular sinuate phytolith examples.
The problem with identifying spices in the prehistoric record is twofold; first plant tissues only rarely preserve and second it is difficult to establish their culinary use. One line of research that is helping in understanding the origins of spice use is that of plant microfossil analysis. For example, starches are reported to survive well in carbonised and non-carbonised residues from a range of prehistoric tools and containers, as well as dental calculus –. In Asia, starch granules from spices, such as ginger and tumeric, have been extracted from nearly four and a half thousand year old Harappan cooking pots . The recovery of phytoliths from carbonised deposits on the inside of potsherds offers the additional possibility to identify leafy, or woody seed material used as spices, which would not be detectable using starch analysis. Phytoliths charred by cooking have been found to be more resilient to destruction at pH extremes, ie. <pH3 and >pH9 . Furthermore, the close association of phytoliths with cooking pots and with other organic traces of food within the charred deposit places the culinary interpretation beyond doubt. Here we report on the analysis of phytoliths from carbonised deposits adhering to the inside of Northern European cooking pots, dating from ca. 6,100 cal BP to 3,750 cal BP, and across the transition from hunting and gathering to farming.
Turning the resolution of analyses to the microscopic level opens a new prospect for documenting a wide variety of plant food. Phytoliths are rigid silica bodies produced by plants following the uptake of silicic acid (Si(OH)4) from the soil , , and a genetically and environmentally controlled deposition in the cells . This deposition can happen in the cells of different plant tissues, often allowing for the identification of both the taxon and the utilised part , . Phytolith research in prehistory has been successfully applied to understanding diet and past plant use in many parts of the world [e.g. 29–31]. Though rarely used as a culinary and paleoenvironmental indicator in northern Europe, phytoliths have made important contributions to debates about the role of plants in other temperate regions, such as China. Early origins (ca. 10,300 cal BP) for common millet (Panicum miliaceum) domesticates have been established on the basis of husk phytoliths in northern China , and rice cultivation has been suggested in 13,000 year old sedimentary sequences from the Yangtze River valley –. The applications of plant microfossil techniques, such as phytolith analysis, are also pushing back the date for the introduction of other important food products, like North American maize (Zea mays) . So far, however, phytoliths have not been used to investigate the antiquity of non-staple crops.
Phytoliths were isolated from 26 out of 74 carbonised deposits recovered from the inside of pots from three sites in Denmark and Germany that span the transition to agriculture (see Materials And Methods) (Figure 1). Moreover, phytoliths were at significantly greater relative abundance from the interior carbonized deposits (n = 61) compared to exterior control deposits (n = 13), (t = 1.99 p = <0.001), showing that they were the direct result of culinary practice (see Phytolith Counts, Figure S1). Globular sinuate phytoliths from seed epidermal tissues  (Figure 1) were found in eight archaeological samples, ranging in number from 1 to 32 per mg of deposit (Table 1).
Five samples are from the coastal site of Neustadt and include both pointed bottomed vessels, typical of the Late Mesolithic Ertebølle culture, and flat/rounded bottomed vessels typical of the Neolithic Funnel beaker culture (Figure 1). On the basis of lipid profiles and single compound isotopic characterisations these samples are associated with hunted resources such as marine, as well as terrestrial ruminant foods (Table 2). Taking a possible reservoir effect into consideration , their date is at least as old as c. 5,900 cal BP. This implies that they are probably contemporary with or younger than the local introduction of domesticated cattle and goat/sheep at 6,200–6,100 cal BP , but represent a use associated with hunted and gathered resources. Two further examples of ceramics associated with globular sinuate phytoliths were from pots found at the inland settlements of Åkonge and Stenø. The former derive from a context dating to c. 5,900 cal BP, which is contemporary with the earliest evidence of domesticated animals in this area . The latter is from a context dated to ca. 6,100 cal BP, and thus is probably earlier than the regional introduction of domesticates and Neolithic pottery here. The residues on the surface of these wetland ceramics are less thick because the organic preservation has been compromised by modern drainage schemes, which may partly account for a reduced phytolith recovery compared to the samples from Neustadt.
Based on the database of the BioPal collection (CaSES-Barcelona), augmented by 20 additional northern European specimens, totalling more than 120 European and Asian plants comprising stems, leaves, and seeds, morphologically equivalent phytoliths have been found only in the seed of modern garlic mustard (Alliaria petiolata,(M.Bieb) Cavara & Grande) (Figure 1) (average phytolith diameter 6.98 μm, range 4.8 μm to 11.2 μm). This plant is found from Europe to Central Asia, northern India and west China, and has a strong peppery, mustard flavour caused by the presence of volatile aglycones in both the edible leaves and seeds . In addition to the phytoliths, all but one of the samples contained lipids from a range of marine and terrestrial animals [42, Table 2] as well as starchy plant foods . These latter food types would provide the consumer with the bulk of the energy and macronutrient requirements, as garlic mustard has little nutritional value. At Neustadt, marine oils predominate in most samples with a lesser contribution from ruminant flesh, whilst ruminant and possibly other terrestrial animal products were spiced at the inland locations.
Discussion and Conclusion
Despite the modest number of samples, it is demonstrated beyond doubt that the use of spice was practised regularly during the decades when domesticates were introduced in the western Baltic region. Although garlic mustard is a locally available source of spice, it is still uncertain if this practise was the result of Neolithic influence ultimately derived from the Near East, from where Old World farming originates, or if such advanced culinary practice was developed locally prior to the arrival of Neolithic elements in northern Europe. The ambiguity is partly due to problems in correction for reservoir effects in food residues where aquatic elements appear to be significant ingredients. In the western Baltic region a reservoir effect of up to 600 14C years has to be accounted for in food derived from marine and freshwater systems , , . The problem makes it challenging to determine if our samples, taken from the hunter-gatherer type pottery at Neustadt, are older than the earliest dates for domesticated animals and plants at the site.
There is no such problem with the context date for the sample from Stenø in Denmark. It clearly predates the introduction of domesticates to the area. Here however, there is only one radiocarbon date available, and it cannot presently be determined with certainty if this date is representative of the whole assemblage, including the ceramic sherd from which the sample was taken. Nevertheless, the present study demonstrates that plant microfossil analysis has opened a new avenue in the study of prehistoric culinary practice in northern European temperate climates. Further, it is now established that the habit of enhancing and altering the flavour of calorie rich staples was part of European cuisine as far back as the 7th millennia cal BP.
Materials and Methods
Permission was obtained from the museums of Holbæk, Kalunborg and Schleswig-Holstein for the removal of foodcrust samples from the sherds. These samples were donated to the project for destructive study. Foodcrusts were scraped from sherds using a clean scalpel. Weighed residues (∼1 mg) were treated with H2O2; 10%, 10 ml; 15–30 min and disaggregated. Samples were centrifuged (2665 RCF; 3 min) and the supernatant removed. The remaining residues were washed three times with UltraPure water and made up to 1ml suspensions. The supernatant, containing liberated phytoliths was added to microscope slides and left to dry (18°C). Samples were mounted in glycerol before viewing in rotated planes using an Olympus IX71 inverted microscope (Olympus, UK) fitted with a ColorView III camera (Olympus, UK) linked to Digital Image Solutions program CellD 2.6 (Build 1200) (Olympus, UK). Silica body counts were normalised and reported per mg of carbonised deposit. Interior (F) and exterior (S) silica body counts were compared using a two-tailed t-test, to establish whether there were significantly higher numbers on the interior, indicative of a deliberate packing of the pots with plant food. Identifications were not carried out on samples with <33 counts mg−1, which corresponds to the maximum count on the exterior deposits.
The lipid analysis followed established protocols –. A total lipid extract (TLE) was obtained through solvent extraction of either ceramic powder (approximately 1 g), drilled from the interior surface of each potsherd, or crushed surface residue (15 mg). An aliquot of each TLE was silylated and analysed by gas chromatography-mass spectrometry (GC-MS). Another aliquot of the TLE was methylated for the analysis of fatty acid methyl esters (FAMEs). An aliquot of the FAME fraction was analysed by GC-MS analysis and another aliquot by GC-combustion-isotope ratio MS (GC-c-IRMS) to obtain a δ13C value for the two major saturated free fatty acids, with 16 and 18 carbon chain lengths.
Radiocarbon dates from Neustadt were made on both charcoal associated with the vessel N_1495 (5122±63 bp, 6000–5700 cal BP (2σ) (KIA-39760)), and on charred foodcrust from N_629 (5460±90 bp, 6450–6000 cal BP (2σ) (AAR-11409), 5350±80 bp, 6300–5950 cal BP (2σ) (AAR-11410)). At Stenø, the context from which the pottery derived was dated to 5250±40 bp, 6200–5950 cal BP (2σ) (Poz-31049) using terrestrial mammal bone. Three directly dated samples of carbonised material from the ceramic matrix were made at Åkonge: ‘Peter’s Pot’ (5140±70 bp, 6200–5700 cal BP (2σ) (AAR-4395)) , 49.5/77.0:18 (5155±40 bp, 6000–5800 cal BP (2σ) (AAR-4817)), and 49.5/77.0:26 (5095±45 bp, 5950–5750 cal BP (2σ) (AAR-5363)). A further four radiocarbon dates were made on ‘sooty’ exterior deposits from Åkonge: 49.5/77.5:10 (5140±40 bp, 6000–5800 cal BP (2σ) (AAR-5111), 50.0/75.5:18 (5070±45 bp, 5950–5750 cal BP (2σ) (AAR-5113), 49.5/77.0:18 (5195±40 bp, 6200–5900 cal BP (2σ) (AAR-4816)), and 49.5/77.0:26 (5195±45 bp, 6200–5800 cal BP (2σ) (AAR-5109)) . In addition several samples of terrestrial mammal bone, including bones of domestic cattle, were dated within the time interval 5120±40 to 4950±60 bp, 5980–5810 cal BP (2σ) .
There is a significant difference (t = 1.99 p = <0.001) in phytolith counts between interior carbonised (n = 61) and exterior soot (n = 13) supporting the claim that vessels with high counts were from the deliberate preparation of plants within the ceramics. The graph shows those samples with high silica body counts (>33 mg−1, green columns) that qualified for further phytolith identification analysis.
We thank Val Steele and Carl Heron who contributed to the lipid analysis, Meg Stark who provided technical support for the SEM analysis and Niels Wickman (Holbæk Museum/Museum of West Zealand) for providing the AMS date from Stenø.
Conceived and designed the experiments: OC HS. Performed the experiments: HS OC. Analyzed the data: HS OC MM. Contributed reagents/materials/analysis tools: AG SH AF. Wrote the paper: HS OC AF.
Smith A (1999) The Wealth of Nations. London: Everyman.
- 2. Andrews AC (1949) Celery and parsley as foods in the Greco-Roman period. Class Philol 44 (2): 91–99.
Freedman P (2008) Out of the East: Spices and the Medieval Imagination. New Haven & London: Yale University Press.
Turner J (2004) Spice: the History of a Temptation. London: Harper Collins.
Strabo, Sterrett JRS (1917) The Geography of Strabo. London: Heinemann.
Mason L (2004) Food and Culture in Great Britain. Westport: Greenwood Press.
- 7. Livarda A (2011) Spicing up life in northwestern Europe: exotic food plant imports in the Roman and medieval world. Veg Hist Archaeobot 20(2): 143–164.
- 8. Schultze-Motel J (1979) Die urgeschichtlichen Reste des Schlafmohns (Papaver somniferum L.) und die Entstehung der Art. Kulturpflanze 27: 207–215.
Knörzer KH (1991) Deutschland nördlich der Donau. In: Van Zeist W, Wasylikowa K, Behre K-E, editors. Progress in Old World palaeoethnobotany. A retrospective view on the occasion of twenty years of the International Work Group of Palaeoethnobotany. Rotterdam: Balkema. 189–206.
Wasylikowa K, Cârciumaru M, Hajnalová E, Hartyányi BP, Pashkevich GA, et al.. (1991) East-Central Europe. In: Van Zeist W, Wasylikowa K, Behre K-E, editors. Progress in Old World Palaeoethnobotany. A Retrospective View on the Occasion of Twenty Years of the International Work Group of Palaeoethnobotany. Rotterdam: Balkema. 207–239.
Mellart J (1970) Excavations at Hacilar, Volume 1. Edinburgh: Edinburgh University Press.
- 12. Hillman G (1975) The plant remains from Tell Abu Hureyra: a preliminary report. Proc Prehist Soc 41: 70–73.
Jacomet S, Brombacher C, Dick M (1991) New Light on Early Farming: Recent Developments in Palaeoethnobotany. Edinburgh: Edinburgh University Press. 257–276.
- 14. Kislev ME (1988) Nahal Hemar Cave, desiccated plant remains: an interim report. Atiqot 18: 76–81.
Koch E (1998) Neolithic Bog Pots from Zealand, Møn, Lolland and Falster. Copenhagen: Det Konglige Nordiske Fortidsminder Serie B.
- 16. Van Zeist W, Bakker-Heeres JAH (1985) Archaeobotanical studies in the Levant, 1. Neolithic sites in the Damascus Basin: Aswad, Ghoraifé, Ramad. Palaeohistoria 24: 165–256.
Zohary D, Hopf M (2004) Domestication of Plants in the Old World. Oxford: Oxford University Press.
- 18. Rowley-Conwy P (1984) The laziness of the short-distance hunter: the origins of agriculture in western Denmark. Journal of Anthropological Archaeology 3: 300–324.
- 19. Mickleburgh HL, Pagán-Jiménez JR (2012) New insights into the consumption of maize and other food plants in the pre-Columbian Caribbean from starch grains trapped in human dental calculus. J Archaeol Sci 39: 2468–2478.
- 20. Allen MS, Ussher E (2012) Starch analysis reveals prehistoric plant translocations and shell tool use, Marquesas Islands, Polynesia. J Archaeol Sci 40: 2799–2812.
- 21. Liu L, Ge W, Bestel S, Jones D, Shi J, et al. (2011) Plant exploitation of the last foragers at Shizitan in the Middle Yellow River Valley China: evidence from grinding stones. J Archaeol Sci 38: 3524–3532.
- 22. Chandler Ezell C, Pearsall D, Zeidler JA (2006) Root and tuber phytoliths and starch grains document manioc (Manihot esculenta) arrowroot (Maranta arundinacea) and llerén (Calathea sp.) at the real alto site Ecuador. Econ Bot 60: 103–120.
- 23. Zarillo S, Pearsall DM, Raymond JS, Tisdale MA, Quon J (2008) Directly dated starch residues document early formative maize (Zea mays L.) in tropical Ecuador. Proc Natl Acad Sci USA 105(13): 5006–5011.
Kashyap A, Weber S (2010) Harappan plant use revealed by starch grains from Farmana, India. Antiquity 84(326): Available: http://antiquity.ac.uk/projgall/kashyap326/. Accessed 2012 Aug 25.
Piperno D (2006) Phytoliths: a comprehensive guide for archaeologists and paleoecologists. Lanham, New York, Toronto, Oxford: Alta Mira.
- 26. Feng Ma J, Yamaji N (2006) Silicon uptake and accumulation in higher plants. Trends Plant Sci 11(8): 392–397.
- 27. Madella M, Jones MK, Echlin P, Powers-Jones A, Moore M (2009) Plant water availability and analytical microscopy of phytoliths: implications for ancient irrigation in arid zones. Quat Int 193(1–2): 32–40.
Madella M (2007) The silica skeletons from the anthropic deposits. In: Whittle A, editor. The Early Neolithic On The Great Hungarian Plain – Investigations of the Körös culture site of Ecsegfalva 23, County Békés. Cardiff and Budapest: Archaeological Institute of the Hungarian Academy of Sciences and Cardiff School of History and Archaeology. 447–460.
- 29. Piperno DR, Andres TC, Stothert KE (2000) Phytoliths in Cucurbita and other neotropical Cucurbitaceae and their occurrence in early archaeological sites from the lowland American tropics. J Archaeol Sci 27: 193–208.
- 30. Piperno DR, Stothert KE (2003) Phytolith evidence for Early Holocene Cucurbita domestication in southwest Ecuador. Science 29: 1054–1056.
Rosen AM (1992) Preliminary identification of silica skeletons from Near Eastern archaeological sites: an anatomical approach. In Rapp G, Mulholland SC, editors. Phytolith Systematics. New York: Plenum Press. 129–147.
- 32. Lu H, Zhang J, Liu K-B, Wu N, Li Y, et al. (2009) Earliest domestication of common millet (Panicum miliaceum) in East Asia extended to 10,000 years ago. PNAS 106(18): 7367–7372.
- 33. Liu L, Lee GA, Jiang L, Zhang J (2007) Evidence for the early beginning (c. 9000 cal. BP) of rice domestication in China: a response. The Holocene 17(8): 1059–1068.
- 34. Lu H, Liu Z, Wu N, Berné S, Saito Y (2002) Rice domestication and climate change: evidence from East China. BOREAS 31(4): 378–385.
- 35. Zhao Z, Piperno D (2000) Late Pleistocene/Holocene environments in the Middle Yangtze River Valley, China and rice (Oryza sativa L.) domestication: the phytolith evidence. Geoarch 15(2): 203–222.
- 36. Hart JP, Thompson RG, Brumbach HJ (2003) Phytolith evidence for early maize (Zea mays) in the Northern Finger Lakes Region of New York. Am Antiq 68(4): 619–640.
- 37. Madella M, Alexandre A, Ball T (2005) International Code for Phytolith Nomenclature 1.0. Ann Bot 96: 253–260.
- 38. Fischer A, Heinemeier J (2003) Freshwater reservoir effect in 14C dates of food residue on pottery. Radiocarbon 45(3): 449–466.
Hartz S (2011) From pointed bottom to round and flat bottom – tracking early pottery from Schleswig-Holstein. Bericht der Römish-Germanishes Kommision vol. 89, 241–276.
Fischer A (2002) Food for feasting? In: Fischer A, Kristiansen K, editors. The Neolithisation of Denmark: 150 Years of Debate. Oxford: Oxbow. 343–393.
Simonetti G (1990) Simon & Schuster's Guide to Herbs and Spices. Florida: Fireside.
- 42. Craig OE, Steele VJ, Fischer A, Hartz S, Andersen SH, et al. (2011) Ancient lipids reveal continuity in culinary practices across the transition to agriculture in Northern Europe. Proc Natl Acad Sci USA 108(44): 17910–17915.
- 43. Saul H, Wilson J, Heron CP, Glykou A, Hartz S, et al. (2012) A systematic approach to the recovery and identification of starches from carbonised deposits on ceramic vessels. J Archaeol Sci 39: 3483–3492.
- 44. Fischer A, Olsen J, Richards M, Heinemeier J, Sveinbjörnsdóttir AE, et al. (2007) Coast-inland mobility and diet in the Danish Mesolithic and Neolithic: evidence from stable isotope values of humans and dogs. J Archaeol Sci 34: 2125–2150.
- 45. Olsen J, Rasmussen P, Heinemeier J (2009) Holocene temporal and spatial variation in the radiocarbon reservoir age of three Danish fjords. BOREAS 38(3): 458–470.
- 46. Dudd SN, Evershed RP (1998) Direct demonstration of milk as an element of archaeological economies. Science 282: 1478–1481.
- 47. Craig OE, Forster M, Andersen SH, Koch E, Crombé P, et al. (2007) Molecular and isotopic demonstration of the processing of aquatic products in northern European prehistoric pottery. Archaeometry 49(1): 135–152.
- 48. Hansel FA, Copley MS, Madureiraa LAS, Evershed RP (2004) Thermally produced omega-(o-alkylphenyl)alkanoic acids provide evidence for the processing of marine products in archaeological pottery vessels. Tetrahedron Lett 45: 2999–3002.