The common zoonotic pathogen Campylobacter coli is an important cause of bacterial gastroenteritis worldwide but its evolution is incompletely understood. Using multilocus sequence type (MLST) data of 7 housekeeping genes from a national survey of Campylobacter in Scotland (2005/6), and a combined population genetic-phylogenetics approach, we investigated the evolutionary history of C. coli. Genealogical reconstruction of isolates from clinical infection, farm animals and the environment, revealed a three-clade genetic structure. The majority of farm animal, and all disease causing genotypes belonged to a single clade (clade 1) which had comparatively low synonymous sequence diversity, little deep branching genetic structure, and a higher number of shared alleles providing evidence of recent clonal decent. Calibration of the rate of molecular evolution, based on within-species genetic variation, estimated a more rapid rate of evolution than in traditional estimates. This placed the divergence of the clades at less than 2500 years ago, consistent with the introduction of an agricultural niche having had an effect upon the evolution of the C. coli clades. Attribution of clinical isolate genotypes to source, using an asymmetric island model, confirmed that strains from chicken and ruminants, and not pigs or turkeys, are the principal source of human C. coli infection. Taken together these analyses are consistent with an evolutionary scenario describing the emergence of agriculture-associated C. coli lineage that is an important human pathogen.
Citation: Sheppard SK, Dallas JF, Wilson DJ, Strachan NJC, McCarthy ND, Jolley KA, et al. (2010) Evolution of an Agriculture-Associated Disease Causing Campylobacter coli Clade: Evidence from National Surveillance Data in Scotland. PLoS ONE 5(12): e15708. https://doi.org/10.1371/journal.pone.0015708
Editor: Rudy A. Hartskeerl, Royal Tropical Institute, Netherlands
Received: September 2, 2010; Accepted: November 22, 2010; Published: December 15, 2010
Copyright: © 2010 Sheppard et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The work was funded by the Food Standards Agency of Scotland and DEFRA. SKS and MCJM are funded by the Wellcome Trust. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Members of the genus Campylobacter are among the most common cause of bacterial gastroenteritis worldwide. The species that are principally responsible for human disease, Campylobacter jejuni and Campylobacter coli , cause sporadic infection  and occasional outbreaks  usually associated with consumption of contaminated meat, poultry, water and contact with animals , . Although C. coli is responsible for fewer food-borne illnesses than C. jejuni, the impact of C. coli is still substantial: of approximately 340000 annual cases of campylobacteriosis in the UK and 2.5 million in the US , , , approximately 10% are caused by C. coli . Therefore, based upon recent estimates, C. coli infection has an annual cost of £50 million in the UK  and $800 million in the USA  but despite the economic importance of this pathogen, most Campylobacter research focuses upon C. jejuni.
Multi-locus sequence typing (MLST) is a molecular typing technique that has enhanced studies of the population structure and epidemiology of Campylobacter . An MLST scheme has been developed for C. coli ,  which characterizes allelic orthologues of the same seven housekeeping gene loci as the original C. jejuni MLST system . The inherent reproducibility of this nucleotide sequence based method and the ability to compare data from different laboratories has enabled the assembly of large archives of isolate genotype data  which provide a valuable resource for analysing the epidemiology and evolution of these organisms.
To date, studies of the genetic structure of C. coli populations have suggested that there is less genetic diversity than in C. jejuni isolate collections from a comparable sample frame , , . However, sufficient variation in MLST alleles exists to identify correlation with the animal host from which the isolate was sampled ,  and recent analyses have exploited this host-genotype relationship to investigate potential disease reservoirs. By describing spatiotemporal patterns and lineage associations in animal hosts ,  and using computer models for the attribution of clinical isolates to source , , ,  these studies consistently link genotypes from clinical isolates with the greatest probability to ruminant and especially chicken (55–80% of isolates) sources. In contrast to C. jejuni, where there is little evidence of deep branching phylogenetic structure, C. coli is divided into three clades . There is evidence that the clades are associated with sample source, with clades 2 and 3 more common in environmental waters , but little is known about the clades, for example when they arose, what is their ecological significance and, in particular, how they relate to clinical infection caused by C. coli.
Using isolate genotype data from a national survey, the Campylobacter MLST project in Scotland - CaMPS (2005/6), we aimed to investigate the disease causing C. coli by analysing the genetic structure of isolates from a variety of sources including clinical infection, farm animals and the environment and using population genetic techniques to characterise the lineages associated with human disease. C. coli was studied by investigating (i) the phylogenetic relationships among isolates (ii) the intrinsic genetic differences between C. coli and C. jejuni lineages, (iii) the quantitative attribution of clinical isolates to different sources, and (iv) the rate of molecular evolution and the time of species and clade divergence. Taken together, these analyses provide evidence for an evolutionary scenario that describes the emergence of clinically important C. coli.
Clinical and potential source isolates
There were 5,674 isolates from confirmed clinical cases of campylobacteriosis, received from 15 health board regions in Scotland between July 2005 and September 2006. Of these, 427 isolates were excluded because they comprised mixed cultures, incomplete typing or other Campylobacter species such as C. lari and C. upsaliensis. Of the remaining clinical isolates (5247), MLST confirmed that 4747 were C. jejuni and 500 were C. coli. In addition to isolates from human disease, a total of 200 C. coli isolates from samples from potential source populations were typed at 7 loci. These were augmented with archive data (1023 isolates) from published sources , , , , , ,  to give a total of 1223 isolates from potential sources. Isolates were grouped by source/host animal to give isolate datasets for phylogenetic and attribution analysis from 98 cattle, 54 sheep, 514 chicken, 380 swine, 110 turkey and 67 riparian (water fowl and environmental waters) sources (Table S1).
There were a total of 451 STs, 103 from clinical isolates and 393 from other sources. The 10 most common STs in clinical (ST-827, ST-825, ST-1774, ST-855, ST-829, ST-1614, ST-872, ST-962, ST-828, ST-1773) and non-clinical (ST-827, ST-825, ST-1068, ST-829, ST-855, ST-854, ST-1101, ST-1614, ST-1017, ST-962) datasets accounted for 72% and 37% of genotypes respectively. Two clonal complexes were present, defined as in the standard definition as groups of STs that share 4 or more alleles in common with the central genotype. Eighty-one percent and 1% of clinical isolates belonged to the ST-828 complex and the ST-1150 complexes respectively and 52% and 4% of non-clinical isolates belonged to these complexes. The remainder of isolates did not belong to a known clonal complex. The clonal complex structure within C. jejuni is greater compared to data sets of a similar magnitude from comparable sources , and this allows the identification of clonal complexes with different levels of host association , . Similar association analysis was not possible for C. coli at the clonal complex level but STs belonging to the ST-828 complex have previously been recovered from clinical disease isolates and from agricultural sources , . There was some variation in allelic diversity by locus (Table S2) but it was generally low, with the total number of STs (451) approximately equal to the number of alleles (410) suggesting that the variation in genotypes results more from re-assortment of existing alleles than generation of new ones by point mutation which would give more alleles per locus. The clinical isolate population had different genetic properties (lower diversity) with the mean number of alleles per locus (13) lower than in the non-clinical data (55).
Clonal frame genealogy
The genealogy determined using CLONALFRAME showed a high degree of genetic structuring in isolates sampled from clinical infection animal sources and the riparian environment (Figure 1). The 3-clade structure that has previously been described  was evident. Comparison of genotypes from clinical infection with this genealogy demonstrated that all of the cases of human C. coli infection were caused by lineages belonging to clade 1 (Table 1). Eighty-four percent of STs from clade 1 belonged to the ST-828 clonal complex.
STs from farm only (green), riparian only (blue), clinical only (red) and farm and clinical (black) sources are indicated by different colours.
ML trees were determined for each of the 7 MLST loci for C. jejuni and C. coli (clades 1–3), and C. jejuni and C. coli combined (data not shown). The topology of the trees for C. jejuni sequences showed no evidence of congruence, with alleles frequently changing position amongst trees. For the combined C. jejuni/C. coli trees there was congruence, partitioning alleles in accordance with species. Similarly, with C. coli there was congruence within the three clades. Visual representation of congruence indicated that within C. coli STs, alleles are more likely to be associated with those from the same clade. Quantitative analysis of congruence was performed using the SH test on the ML trees for combined C. coli data, C. jejuni/C. coli, C. jejuni, and the three C. coli clades separately (Figure 2). Within C. jejuni there was no evidence of congruence with the likelihood values (-ln L) for all of the single locus trees within the range of -ln L values generated for random trees (Figure 2B). This suggests extensive recombination. The -ln L for combined C. jejuni/C. coli trees provided evidence of tree congruence for aspA, gltA, glyA and tkt (Figure 2C). This is expected as the likelihood of one single locus tree predicting another is high when they share a distinct two-species distribution. Within C. coli the congruence between single locus trees, suggested by ST restriction within clades, was confirmed with likelihood values for congruence between competing ML trees outside of the –ln L values for random trees for all 7 MLST loci. This suggests relatively low levels of recombination between clades. Analysis within individual C. coli clades showed no evidence of congruence indicative of recombination within clades.
The ML tree of each MLST locus is compared with the ML trees from the other six loci for: (A) C. coli clades 1–3, (B) C. jejuni, (C) C. coli and C. jejuni (D) C. coli clade1, (E) C. coli clade 2 and (F) C. coli clade 3. The tree congruence likelihood (ln L) range is shown with horizontal bars for each locus (black) and for 200 trees of random topology (grey) for aspA, glnA, gltA, glyA, pgm, tkt and unc respectively from top to bottom on each graph. If the ln L range falls outside the range calculated for random trees there is more congruence among the ML trees than expected by chance.
Molecular clock estimates of clade divergence
The C. jejuni population from the 3 year longitudinal study  that was used to calibrate the tree contained sufficient levels of mutation, recombination or coalescence events to estimate the timescale of the genealogy (Neg), where Ne is the effective population size and g is the generation length. The rate of molecular change was analysed using the importance sampler  and the results of the three alternative datasets were merged to produce a model average over the datasets. There was negligible uncertainty in the tree topology (Figure 3), and the topology was as expected from separate analyses . The uncertainty in the scale bar, which represents uncertainty in the calibration of the molecular clock, was (2719–9194 years) for a scale bar of length 5000 years. The point estimates for the divergence of the different Campylobacter lineages were consistent with previous estimates  and placed the divergence of C. coli and C. jejuni at 6429 (95% CI, 6280–6579) years ago with C. coli clade 3 diverging approximately 1684 (95% CI, 1659–1709) years ago and clades 1 and 2 diverging approximately 1023 (95% CI, 1005–1041) years ago (Table 2).
Error bars, in grey, associated with each node indicate relative uncertainty in node height. Calibration of the scale bar was based on intraspecific variation in Campylobacter jejuni and uncertainty associated with the molecular clock calibration is represented by a 95% CI below the scale bar. Empirical and indirect  estimates of the rate of molecular evolution would calibrate the same scale bar at 42,200 (95% CI 2,690–661,000) and 7,600,000 years respectively.
The traditional method for dating recent bacterial evolution ,  is to calibrate the rate of sequence divergence relative to the split of E. coli and Salmonella typhimurium which Ochman and Wilson estimated at 120–160 million years ago (Ma), based on 1% divergence in the 16S rRNA gene per 50 million years . By this estimate the C. jejuni-C. coli split would have occurred approximately 10 Ma , very different to the estimate (6479 years) using our method, based on intra-specific variation. By our estimate, speciation is occurring over thousands rather than millions of years. The root of the tree was dated at 38,269 years ago (95% CI 20198–73764) and the speciation rate (λ) was 0.054 (95% CI 0.019–0.14) per lineage per 1000 years . Estimates of mutation rate, µ = 0.029, (95% CI 0.016–0.053), the transition-transversion ratio, κ = 2.86 (95% CI 2.47–3.31), and dN/dS ratio, ω = 0.011 (95% CI 0.009–0.014) were in good agreement with previous estimates for this genus . To enable comparison of our molecular clock calibration based on intraspecific variation with other estimates, the time represented by the scale bar in figure 3 was determined using other calibration methods. With an empirical estimate, based on published generation times ,  and genomic mutation rates  for C. jejuni, the scale bar would represent a period of 42,200 years (95% CI 2,690–661,000) and with the Ochman and Wilson method  the scale bar would represent 7.6 My .
Attribution to putative source
Isolates from known sources were used to test the limitations of the attribution model as previously described . Random subsets of the comparison datasets for all putative source populations were used for self-assignment. Test sets of 50% of the swine, ruminant, chicken, turkey and riparian isolates were assigned to host source based on a reduced training set, and the AI model assigned them with 94%, 89%, 95%, 92%, 88% probability to the correct source: swine, ruminant, chicken, turkey and riparian sources respectively. For each clinical isolate the probability of assignment to each potential source was calculated and the sum of these probabilities was used to determine the percentage of all clinical isolates that are attributed to each source. The clinical C. coli isolates were attributed to source in the following percentages: 57% to poultry, 41% to ruminant, 1% to swine, 0.5% to turkey and 0.5% to riparian sources.
The genealogical reconstruction of genotypes from diverse sources demonstrated the clade structure and revealed that all of human C. coli disease cases were caused by STs belonging to clade 1. The 3-clade structure, and its relationship to disease, is poorly understood but for it to arise and be maintained requires differentiated gene pools. The simplest explanation for this is a general reduction in the overall level of recombination but there is evidence of frequent recombination within each clade (Figure 2), and therefore it is likely that recombinational barriers are involved in clade divergence. Three broad classes of barrier can be described, (i) mechanistic barriers - imposed by the homology dependence of recombination  or other factors promoting DNA specificity such as restriction/modification systems ; (ii) ecological barriers - a consequence of physical separation of bacterial populations in distinct niches; (iii) adaptive barriers - implying selection against hybrid genotypes . The relative importance of these three different classes of barrier is unclear but evidence from interspecies recombination between C. coli and C. jejuni  is consistent with the involvement of factors such as physical proximity, a shared vector (bacteriophage) infecting both lineages, or niche adaptation playing a role.
C. coli, clade 1 has several genetic characteristics that distinguish it from other lineages within C. coli. First, this clade has relatively low synonymous sequence diversity in MLST genes (mean Ds of 0.006 per nucleotide) compared to C. jejuni (0.016) and clades 2 (0.008) and 3 (0.013). The sequence diversity is higher than that of organisms commonly described as genetically monomorphic (Ds of <0.0002), for example enteric Salmonella Typhi or Yersinia pestis , but is still comparatively low indicating a relatively recent genetic bottleneck. Second, on a tree based on concatenating multiple genetic regions from each strain, in this case MLST data, there is little evidence of deep genetic structure that would indicate long periods of independent evolution of clade 1 (or clade 2). Third, there is more evidence of genetic exchange within C. coli clade 1 as STs often share the same allele at individual MLST loci and as in other bacterial species, this sharing of the majority of alleles is strong evidence of recent clonal descent.
The importance of recombination in generating variation also varies among the clades. The relative importance of the re-assortment of existing alleles (recombination) and the generation of new ones (mutation), in producing variation can be provided by comparison of the number of alleles at each locus, ranging in this study from 120 for aspA to 221 for pgm, with the number expected for the number of STs (1441) if point mutation generating novel alleles were responsible for the diversity (1441/7 = 206). If the observed number of alleles is lower than this expected value then recombination is involved in generating diversity. Using this method the ratio of observed to expected alleles was 0.5 for C. coli clade 1 and 0.7 for C. jejuni compared to 2.6 and 4.9 for clades 2 and 3 respectively. This indicates that recombination is 5–10 times more important in generating diversity in C. coli clade 1 than in the other clades. Recent work that uses combined population-genetic microevolutionary models demonstrates that recombination generates twice as much diversity as de novo mutation in Campylobacter and has a fundamental role in the evolution of this genus . Our findings for C. coli clade 1 are consistent with these results but demonstrate that the importance of recombination can vary within subgroups of the same species (clades 2 and 3).
The numerical dominance of clade 1 C. coli genotypes from clinical disease can be explained, without consideration of intrinsic differences in the ability to infect humans, as a reflection of the dominance of isolates from this clade in disease reservoirs and food chain sources. Like C. jejuni, there are limitations on how well C. coli lineages can be attributed to source because, while there are host-associated alleles , , there is also considerable niche overlap with the same alleles and STs found in isolates from different farm hosts , . However, alleles from STs clustered into clade 1 are distinct from those in clades 2 and 3 and this level of genetic differentiation allows source attribution. This shows that poultry and ruminants and not pigs and turkeys (clade 1) or the riparian environment (the source of most clade 2 and 3 isolates), are the most likely source of human C. coli infection. This is consistent with evidence of the source of human C. jejuni infection , , . In evolutionary terms, agriculture is a new niche and the co-infection of animals including chicken and ruminants by similar strains, despite the differences in the biology of their digestive tracts, suggests that this niche has acquired specifically adapted lineages of bacteria, rather than sharing a common gene pool with a pre-existing natural reservoir. Agricultural environments are highly unusual in terms of host diet, genetic and age structure, density and many other details of habitation. It appears that this novel niche has been recently colonised by clade 1 C. coli as indicated not only by evidence of a smaller historical population size but also by the nature of recently recombined C. jejuni alleles found in C. coli clade 1. These alleles are typical of those found in C. jejuni from farm sources and are almost all identical to those found in the donor species, indicating that the imports occurred recently enough to have not accumulated mutations .
Determining the timescale of the evolutionary divergence of the three C. coli clades provides a context for understanding the link between ecological factors, such as the domestication of the host niche, and the population genetic structure. However, there is some debate about the rate of bacterial evolution because unlike multicellular eukaryotes they do not leave a morphologically informative fossil record and, unlike viruses, they do not mutate fast enough for evolution of natural populations to be easily measured in real time. Indirect methods calibrate the molecular clock in bacteria by cross-referencing events that can be dated. For example, the common ancestor of mitochondria and their closest living bacterial relatives  or cospeciation (of bacterial endosymbionts) with invertebrate hosts for which a fossil record is available . Estimates based on such methods have been widely used , , ,  but they can conflict with empirical approaches that are based on laboratory measurements of generation lengths and mutation rates , .
Dating Campylobacter evolution with reference to indirect methods for estimating the split of E. coli and S. typhimurium , would place the divergence of the three C. coli clades at approximately 2.5 million years ago. This is incompatible with the hypothesis that agriculture played a part in the divergence of the lineages because modern farming did not begin until around ten thousand years ago (in the Middle East), and was not common throughout Europe until much later (5,000–3,000 BC) , , . However, the more recent estimate for the divergence of the clades, based on intra-specific variation in longitudinally sampled C. jejuni isolates, places the divergence of the C. coli clades at less than 2,500 years ago. This is consistent with the introduction of agriculture having had an effect upon the evolution of the genus Camplylobacter, for example by introducing novel opportunities for pathogens to expand into new host species and to spread rapidly to new territories. There are several potential causes for the conflict between short and long term estimates of bacterial evolution and the debate continues but an increasing number of studies support the theory of a far more rapid rate of bacterial evolution , , , . Furthermore, the impact of agriculture upon bacterial evolution has been demonstrated in Staphylococcus aureus where the majority of isolates from chickens are the descendants of a single human-to-poultry host jump that occurred approximately 38 years ago (range, 30 to 63 years ago) in Poland . This demonstrates the strong global effect that agriculture can have on bacterial evolution and may inform understanding of the population genetic structure of Campylobacter in the context of relatively rapid evolution.
Taken together the population genetic structure, source attribution of isolates and molecular clock calibration for clade divergence are consistent with an evolutionary scenario where a lineage within the C. coli has diverged into an agricultural niche. This may be related to intrinsic genetic differences associated with adaptation and the observation that some C. jejuni strains are more similar to particular strains of C. coli in gene content than they are to other strains of C. jejuni  supports this, but genome-wide studies of patterns of exchange are necessary to investigate the nature of barriers to gene flow and the consequences of recombination for ecological adaptation. Subtypes belonging to the agricultural C. coli lineage (clade 1) are responsible for the majority of human disease. It remains possible that there are genomic differences associated with pathogenicity but these are not required to explain the over representation of this clade among human disease cases. The most likely reason for the dominance of this clade in human disease is that it is an agricultural lineage and human disease is directly linked to food animals in the food chain.
Materials and Methods
Ethical approval (reference: 05/S0802/151) for the collection of the samples and information used in this project was obtained from Grampian Local Research Ethics Committee (Summerfield house, Aberdeen, UK). This was in accordance with government agreements for research ethics committees (July 2001) and in compliance with the standard operating procedures in the UK. Specimens were collected from all 28 NHS clinical diagnostic laboratories in Scotland that agreed to participate. Archived isolate information included submitting laboratory, specimen number and date of collection. In accordance with Grampian Local Research Ethics Committee approval, patients were informed of the survey and had the option to decline. As no information that would allow identification of the patient was collected, individual patient consent was not required from patients that did not decline.
The structured sampling and isolation procedure was carried out as described previously for the Campylobacter MLST project in Scotland (CaMPS), , , . In brief, clinical stool isolates (5674), animal faeces samples (2644) and food samples (394) were collected to represent all reported clinical cases of campylobacteriosis in the 15 health boards in Scotland (July 2005–September 2006) as well as a wide range of potential sources including contaminated food, animal faeces and the environment over several geographical areas and time periods. Twenty-five grams of food and fresh faecal (mammalian) and <5 g avian faecal samples were homogenised (1∶9) in Campylobacter enrichment broth  and plated (0.1 ml) onto charcoal cefoperazone deoxycholate (CCD, CM0739, Oxoid, UK) agar. The presence of Campylobacter was determined by incubating the remaining enrichment broth microaerobically (2% H2, 5% O2, 5% CO2, balance N2) 100-ml volumes of nutrient broth base (Mast, Bootle, UK) with 5% horse blood, growth supplement (Mast Selectavial SV61), amphotericin (2 µg/ml), cefoperazone (15 µg/ml) and trimethoprim (10 µg/ml) at 37°C. After 6–8 h enrichment, two additional antimicrobials (polymixin B, 2500 IU/litre, and rifampicin, 5 µg/ml) were added, and the broths were incubated for a further 5 days. Enrichment broths (0.1 ml) were plated, after 2 and 5 days, on CCD agar (Oxoid, UK) and incubated microaerobically at 37°C . Gram Stain, Microscreen latex agglutination (Microgen, Camberley, UK) and PCR amplification - with primers specific for either C. jejuni or C. coli pgm genes - were used to presumptively identify Campylobacter. Individual isolates were stored at −80°C in nutrient broth with 15% (v/v) glycerol.
Multilocus sequence typing (MLST)
DNA was extracted from isolates recultured microaerobically at 37°C (for 48 h) with a CHELEX resin method (BIO-RAD, USA) as previously described . A high throughput 7-locus MLST protocol was used, based upon a 2-phase robotic system for PCR of template DNA arrays and amplification products using published primers, reagent concentrations, template purification protocols and cycle parameters , , . This process is described in more detail elsewhere , . In brief, following electrophoresis (200 V, 10 min) on agarose gel in 1x TAE buffer (1 mM EDTA, 40 mM Tris-acetate) and UV visualization, 5 µl of the original PCR products were precipitated with 20% polyethylene glycol–2.5 M NaCl  and nucleotide sequencing PCRs (2 µl of DNA, 6.98 µl water, 1.0 µl 5x buffer, 0.02 µl BigDye Terminator v3.1 mix [Applied Biosystems, UK] and 0.1 µM of primer) were performed in both directions with cycling parameters as follows: 30 cycles of 96°C for 10 s, 50°C for 5 s, and 60°C for 2 min. Sequencing PCR products were precipitated, cleaned with 95% ethanol, and analyzed with an ABI Prism 3730 automated DNA sequencer (Applied Biosystems, UK). Forward and reverse sequences were assembled using the Staden suite of computer programs  and allelic orthologs were assigned numbers giving a 7-locus sequence type (ST). Contemporaneous survey data were augmented with data from published studies , , , , , ,  obtained from the publicly accessible MLST database (http://pubmlst.org), hosted by the University of Oxford .
Overview of population genetic analysis
A variety of analytical approaches were used to describe the evolutionary history of C. coli. Ancestral relatedness of genotypes was assessed using CLONALFRAME  to construct genealogies for inference of the C. coli phylogeny. Lineage clonality was inferred by investigating congruence of maximum likelihood trees . The timescale of C. coli evolution was calibrated using the phylogenetic inference package BEAST  and previous estimates of the evolutionary rate in the Campylobacter genus based on longitudinal sequences sampled within C. jejuni . Source attribution of clinical genotypes was determined using the Asymmetric Island (AI) probabilistic genetic attribution model .
The genealogy of the C. coli STs was estimated using a model-based approach for determining bacterial microevolution: CLONALFRAME . Using this model, clonal relationships are calculated with improved accuracy compared with standard phylogenetic inference techniques for recombining bacteria because the two major sources of allelic polymorphisms (point mutation and recombination) are distinguished. This model has been used successfully to distinguish clades within C. coli . Analysis was carried out on all the STs from clinical and non clinical sources. In each case, 7-locus STs were concatenated and the program run with a burn-in of 50,000 iterations followed by 50,000 iterations of sampling. The consensus tree represents combined data from three independent runs with 75% consensus required for inference of relatedness.
The phylogenetic history of bacteria, evolving according to a clonal model, will be the same for all loci within a genome if it represents a single linkage group irrespective of location. The degree of clonality, therefore, can be estimated by measuring the degree of congruence between phlyogenetic trees constructed for different loci from a single genome. This approach has been employed to compare maximum likelihood (ML) trees describing multiple loci from the genome of, for example, Borrelia burgdorferi , Neisseria meningitidis  and Streptococcus uberis . Twenty-six genotypes were selected to produce ML trees as in previous studies . Single locus ML trees were constructed for each locus of sample groups containing C. jejuni, C. jejuni and C. coli, C. coli (12 STs from each clade), C. coli clade 1, C. coli clade 2 and C. coli clade 3 and the congruence between trees was determined using the Shimodaira-Hasegawa (SH) test , . The difference in congruence log-likelihood (Δ-ln L) of the tree topologies was determined and compared for the 7 ML trees. If evolution is entirely clonal then there should be no significant difference in phylogenetic congruence. The extent of congruence was tested further using the randomized test ,  by comparing the log likelihood for the 7 individual locus ML tree topologies with equivalent values for 200 randomly generated trees of the same size for each gene. If there is more congruence among the ML trees than expected by chance alone then the log likelihood values will fall outside the range calculated for random trees. These analyses were performed using PAUP* version 4 .
The timescale of C. coli evolution
The phylogenetic history of C. coli was reconstructed, in the context of other species within the genus Campylobacter, for which similar MLST schemes have been developed , , . As described previously , 4 of the loci (glnA, glyA, tkt and uncA) used in 7-locus STs are common to MLST schemes for all species. STs for each Campylobacter species, C. fetus (ST-4), C. helveticus (ST-2), C. insulaenigrae (ST-12), C. lari (ST-6), C. upsaliensis (ST-25) and 3 STs from C. jejuni (ST-21, ST-257 and ST-2678) and C. coli clades 1 (ST-1132, ST-3177, ST-1245), 2 (ST-3323, ST-3828, ST-3314) and 3 (ST-3310, ST-3309, ST-2681) were tested to confirm the absence of interspecies recombination between selected STs using a permutation test based on the correlation between physical distance and linkage disequilibrium (LD) . These STs were analysed using the Bayesian phylogenetic package BEAST , a codon substitution model  and the Yule model of speciation rates . On the timescale of Campylobacter evolution all of the STs from isolates in this study were effectively sampled at the same time, therefore there was no data for estimating the rate of evolutionary change. To account for this, we utilized informative prior distributions on the evolutionary parameters comprising the transition-transversion ratio, the dN/dS ratio and the synonymous mutation rate. The priors were taken from the parameters inferred from an analysis of a longitudinal sample of C. jejuni collected over a 3-year period  assuming a constant rate of evolution within the genus Campylobacter.
Source attribution of clinical genotypes
The Asymmetric Island (AI) probabilistic genetic attribution model , was used to characterize the population structure from the genetic data and assign individual isolates in the test set of human isolates independently to source using the training data set. This technique has been used previously ,  and the limitations on the attribution accuracy achievable from a 7-locus profile have been validated by calculating the probability of correct ‘self-assignment’ of a randomly selected sub-set of each host species to the correct origin population . The AI program was run with 1,000 iterations of burn-in followed by 10,000 iterations of sampling, for probabilistic assignment. The putative source of 7-locus genotypes from clinical C. coli isolates (500) was assigned by comparison to datasets comprising genotype data from contemporaneous host and environmental/food isolates and genotype data from published sources , , , , , , .
Summary of isolates by ST and source.
NHS diagnostic laboratories and CPHMs, Staff of AU and NHS Grampian for collecting faecal specimens, farmers, LACORS, veterinary laboratories (SAC and UoG), animal rescue centres and wildlife reserves, postgraduate research students, visiting research students.
Conceived and designed the experiments: SKS. Performed the experiments: SKS JFD. Analyzed the data: SKS DJW NDM KAJ. Contributed reagents/materials/analysis tools: NJCS FMC OR IDO KJF MCJM. Wrote the paper: SKS MCJM.
- 1. Friedman CJ, Neiman J, Wegener HC, Tauxe RV (2000) Epidemiology of Campylobacter jejuni infections in the United States and other industrialised nations. In: Nachamkin I, Blaser MJ, editors. Campylobacter. Washington, D.C.: ASM Press. pp. 121–138.
- 2. Olson CK, Ethelberg S, van Pelt W, Tauxe RV (2008) Epidemiology of Campylobacter jejuni infections in industrialized nations. In: Nachamkin I, Szymanski CM, Blaser MJ, editors. Campylobacter. 3 ed. Washington, D.C., : ASM Press. pp. 163–191.
- 3. Pebody RG, Ryan MJ, Wall PG (1997) Outbreaks of campylobacter infection: rare events for a common pathogen. Communicable Disease Reports CDR Reviews 7: R33–37.
- 4. Kapperud G, Espeland G, Wahl E, Walde A, Herikstad H, et al. (2003) Factors associated with increased and decreased risk of Campylobacter infection: a prospective case-control study in Norway. Am J Epidemiol 158: 234–242.
- 5. Friedman CR, Hoekstra RM, Samuel M, Marcus R, Bender J, et al. (2004) Risk factors for sporadic Campylobacter infection in the United States: A case-control study in FoodNet sites. Clinical Infectious Disases 38: Suppl 3S285–296.
- 6. Allos B (2001) Campylobacter jejuni infections: update on emerging issues and trends. Clinical Infectious Diseases 2001: 32: 1201–1206.
- 7. Kessel AS, Gillespie IA, O'Brien SJ, Adak GK, Humphrey TJ, et al. (2001) General outbreaks of infectious intestinal disease linked with poultry, England and Wales, 1992-1999. Commun Dis Public Health 4: 171–177.
- 8. Tam CC, O'Brien SJ, Adak GK, Meakins SM, Frost JA (2003) Campylobacter coli - an important foodborne pathogen. J Infect 47: 28–32.
- 9. Gillespie IA, O'Brien SJ, Frost JA, Adak GK, Horby P, et al. (2002) A case-case comparison of Campylobacter coli and Campylobacter jejuni infection: a tool for generating hypotheses. Emerging Infectious Diseases 8: 937–942.
- 10. Humphrey T, O'Brien S, Madsen M (2007) Campylobacters as zoonotic pathogens: a food production perspective. International Journal of Food Microbiology 117: 237–257.
- 11. Buzby JC, Roberts T (1997) Economic costs and trade impacts of microbial foodbourne illness. World Health Stat Q 50: 57–66.
- 12. Dingle KE, Colles FM, Wareing DRA, Ure R, Fox AJ, et al. (2001) Multilocus sequence typing system for Campylobacter jejuni. Journal of Clinical Microbiology 39: 14–23.
- 13. Dingle KE, Colles FM, Falush D, Maiden MC (2005) Sequence typing and comparison of population biology of Campylobacter coli and Campylobacter jejuni. Journal of Clinical Microbiology 43: 340–347.
- 14. Miller WG, On SL, Wang G, Fontanoz S, Lastovica AJ, et al. (2005) Extended multilocus sequence typing system for Campylobacter coli, C. lari, C. upsaliensis, and C. helveticus. Journal of Clinical Microbiology 43: 2315–2329.
- 15. Maiden MC (2006) Multilocus Sequence Typing of Bacteria. Annual Review of Microbiology 60: 561–588.
- 16. Duim B, Wassenaar TM, Rigter A, Wagenaar J (1999) High-resolution genotyping of Campylobacter strains isolated from poultry and humans with amplified fragment length polymorphism fingerprinting. Applied and Environmental Microbiology 65: 2369–2375.
- 17. Miller WG, Englen MD, Kathariou S, Wesley IV, Wang G, et al. (2006) Identification of host-associated alleles by multilocus sequence typing of Campylobacter coli strains from food animals. Microbiology 152: 245–255.
- 18. Sheppard SK, Colles F, Richardson J, Cody AJ, Elson R, et al. (2010) Host Association of Campylobacter Genotypes Transcends Geographic Variation. Applied and Environmental Microbiology 76: 5269–5277.
- 19. Rotariu O, Dallas JF, Ogden ID, MacRae M, Sheppard SK, et al. (2009) Spatiotemporal homogeneity of Campylobacter subtypes from cattle and sheep across northeastern and southwestern Scotland. Appl Environ Microbiol 75: 6275–6281.
- 20. Sheppard SK, Dallas JF, MacRae M, McCarthy ND, Sproston EL, et al. (2009) Campylobacter genotypes from food animals, environmental sources and clinical disease in Scotland 2005/6. International Journal of Food Microbiology 134: 96–103.
- 21. Wilson DJ, Gabriel E, Leatherbarrow AJH, Cheesbrough J, Gee S, et al. (2008) Tracing the source of campylobacteriosis. PLoS Genetics 26: e1000203.
- 22. Sheppard SK, Dallas JF, Strachan NJ, MacRae M, McCarthy ND, et al. (2009) Campylobacter genotyping to determine the source of human infection. Clinical Infectious Diseases 48: 1072–1078.
- 23. Mullner P, Jones G, Noble A, Spencer SE, Hathaway S, et al. (2009) Source attribution of food-borne zoonoses in New Zealand: a modified Hald model. Risk Anal 29: 970–984.
- 24. Strachan NJ, Gormley FJ, Rotariu O, Ogden ID, Miller G, et al. (2009) Attribution of Campylobacter infections in northeast Scotland to specific sources by use of multilocus sequence typing. Journal of Infectious Diseases 199: 1205–1208.
- 25. Sheppard SK, McCarthy ND, Falush D, Maiden MC (2008) Convergence of Campylobacter species: implications for bacterial evolution. Science 320: 237–239.
- 26. Sopwith W, Birtles A, Matthews M, Fox A, Gee S, et al. (2009) Investigation of food and environmental exposures relating to epidemiology of Campylobacter coli in humans in North West England. Applied and Environmental Microbiology.
- 27. Thakur S, Gebreyes WA (2005) Campylobacter coli in swine production: antimicrobial resistance mechanisms and molecular epidemiology. Journal of Clinical Microbiology 43: 5705–5714.
- 28. Kinana AD, Cardinale E, Bahsoun I, Tall F, Sire JM, et al. (2007) Campylobacter coli isolates derived from chickens in Senegal: Diversity, genetic exchange with Campylobacter jejuni and quinolone resistance. Research in Microbiology 158: 138–142.
- 29. Litrup E, Torpdahl M, Nielsen EM (2007) Multilocus sequence typing performed on Campylobacter coli isolates from humans, broilers, pigs and cattle originating in Denmark. Journal of Applied Microbiology 103: 210–218.
- 30. McCarthy ND, Colles FM, Dingle KE, Bagnall MC, Manning G, et al. (2007) Host-associated genetic import in Campylobacter jejuni. Emerging Infectious Diseases 13: 267–272.
- 31. Fearnhead P (2008) Computational methods for complex stochastic systems: a review of some alternatives to MCMC. Statistics and Computing 18: 151–171.
- 32. Wilson DJ, Gabriel E, Leatherbarrow AJ, Cheesbrough J, Gee S, et al. (2009) Rapid evolution and the importance of recombination to the gastroenteric pathogen Campylobacter jejuni. Mol Biol Evol 26: 385–397.
- 33. Achtman M, Morelli G, Zhu P, Wirth T, Diehl I, et al. (2004) Microevolution and history of the plague bacillus, Yersinia pestis. Proceedings of the National Academy of Sciences USA 101: 17837–17842.
- 34. Roumagnac P, Weill FX, Dolecek C, Baker S, Brisse S, et al. (2006) Evolutionary history of Salmonella typhi. Science 314: 1301–1304.
- 35. Ochman H, Wilson AC (1987) Evolution in bacteria: evidence for a universal substitution rate in cellular genomes. Journal of Molecular Evolution 26: 74–86.
- 36. Yule GU (1924) A mathematical theory of evolution, based on the conclusions of DR. J.C. Willis. Philosophical Transactions of the Royal Society of London Series B: Biological Sciences 213: 21–87.
- 37. Khanna MR, Bhavsar SP, Kapadnis BP (2006) Effect of temperature on growth and chemotactic behaviour of Campylobacter jejuni. Letters in Applied Microbiology 43: 84–90.
- 38. Konkel ME, Christensen JE, Dhillon AS, Lane AB, Hare-Sanford R, et al. (2007) Campylobacter jejuni strains compete for colonization in broiler chicks. Applied and Environmental Microbiology 73: 2297–2305.
- 39. Drake JW (1991) A constant rate of spontaneous mutation in DNA-based microbes. Proceedings of the National Academy of Sciences U S A 88: 7160–7164.
- 40. Fraser C, Hanage WP, Spratt BG (2007) Recombination and the nature of bacterial speciation. Science 315: 476–480.
- 41. Eggleston AK, West SC (1997) Recombination initiation: easy as A, B, C, D… chi? Current Biology 7: R745–749.
- 42. Zhu P, van der Ende A, Falush D, Brieske N, Morelli G, et al. (2001) Fit genotypes and escape variants of subgroup III Neisseria meningitidis during three pandemics of epidemic meningitis. Proceedings of the National Academy of Sciences USA 98: 5234–5239.
- 43. Achtman M, Wagner M (2008) Microbial diversity and the genetic nature of microbial species. Nat Rev Microbiol 6: 431–440.
- 44. Strachan NJ, Gormley FJ, Rotariu O, Ogden ID, Miller G, et al. (2008) Attribution of Campylobacter infections in northeast Scotland to specific sources by use of multilocus sequence typing. Journal of Infectious Diseases 199: 1205–1208.
- 45. Moran NA (2003) Tracing the evolution of gene loss in obligate bacterial symbionts. Curr Opin Microbiol 6: 512–518.
- 46. Zhang W, Qi W, Albert TJ, Motiwala AS, Alland D, et al. (2006) Probing genomic diversity and evolution of Escherichia coli O157 by single nucleotide polymorphisms. Genome Res 16: 757–767.
- 47. Pupo GM, Lan R, Reeves PR (2000) Multiple independent origins of Shigella clones of Escherichia coli and convergent evolution of many of their characteristics. Proceedings of the National Academy of Sciences USA 97: 10567–10572.
- 48. Lenski RE, Winkworth CL, Riley MA (2003) Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations. Journal of Molecular Evolution 56: 498–508.
- 49. Ochman H (2003) Neutral mutations and neutral substitutions in bacterial genomes. Molecular Biology and Evolution 20: 2091–2096.
- 50. Ammerman AJ, Cavalii-Sforza LL (1984) The neolithic transition and the genetics of populations in Europe . USA: Princeton University Press.
- 51. McCorriston J, Hole F (1991) The ecology of seasonal stress and the origin of agriculture in the Near East. American Anthropologist 93: 46–69.
- 52. Zvelebil M, Dolukhanov P (1991) The transition to farming in eastern and northern europe. Journal of World Prehistory 5: 233–278.
- 53. Falush D, Kraft C, Taylor NS, Correa P, Fox JG, et al. (2001) Recombination and mutation during long-term gastric colonization by Helicobacter pylori: estimates of clock rates, recombination size, and minimal age. Proceedings of the National Academy of Sciences USA 98: 15056–15061.
- 54. Perez-Losada M, Crandall KA, Zenilman J, Viscidi RP (2007) Temporal trends in gonococcal population genetics in a high prevalence urban community. Infect Genet Evol 7: 271–278.
- 55. Feng L, Reeves PR, Lan R, Ren Y, Gao C, et al. (2008) A recalibrated molecular clock and independent origins for the cholera pandemic clones. PLoS One 3: e4053.
- 56. Lowder BV, Guinane CM, Ben Zakour NL, Weinert LA, Conway-Morris A, et al. (2009) Recent human-to-poultry host jump, adaptation, and pandemic spread of Staphylococcus aureus. proceedings of the National Academy of Sciences U S A 106: 19545–19550.
- 57. Debruyne L, Gevers D, Vandamme P (2008) Taxonomy of the family Campylobacteraceae. In: Nachamkin I, Szymanski CM, Blaser MJ, editors. Campylobacter. 3rd ed. Washington DC: ASM Press. pp. 3–25.
- 58. Gormley FJ, Macrae M, Forbes KJ, Ogden ID, Dallas JF, et al. (2008) Has retail chicken played a role in the decline of human campylobacteriosis? Appl Environ Microbiol 74: 383–390.
- 59. Humphrey TJ, Jorgensen F, Frost JA, Wadda H, Domingue G, et al. (2005) Prevalence and subtypes of ciprofloxacin-resistant Campylobacter spp. in commercial poultry flocks before, during, and after treatment with fluoroquinolones. Antimicrob Agents Chemother 49: 690–698.
- 60. Embley TM (1991) The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Letters in Applied Microbiology 13: 171–174.
- 61. Staden R (1996) The Staden sequence analysis package. Molecular Biotechnology 5: 233–241.
- 62. Jolley KA, Chan MS, Maiden MC (2004) mlstdbNet - distributed multi-locus sequence typing (MLST) databases. BMC Bioinformatics 5: 86.
- 63. Didelot X, Falush D (2007) Inference of bacterial microevolution using multilocus sequence data. Genetics 175: 1251–1266.
- 64. Dykhuizen DE, Polin DS, Dunn JJ, Wilske B, Preac Mursic V, et al. (1993) Borrelia burgdorferi is clonal: implications for taxonomy and vaccine development. Proceedings of the National Academy of Sciences USA 90: 10163–10167.
- 65. Drummond AJ, Nicholls GK, Rodrigo AG, Solomon W (2002) Estimating mutation parameters, population history and genealogy simultaneously from temporally spaced sequence data. Genetics 161: 1307–1320.
- 66. Holmes EC, Urwin R, Maiden MCJ (1999) The influence of recombination on the population structure and evolution of the human pathogen Neisseria meningitidis. Molecular Biology and Evolution 16: 741–749.
- 67. Coffey TJ, Pullinger GD, Urwin R, Jolley KA, Wilson SM, et al. (2006) First insights into the evolution of Streptococcus uberis: a multilocus sequence typing scheme that enables investigation of its population biology. Applied and Environmental Microbiology 72: 1420–1428.
- 68. Feil EJ, Holmes EC, Bessen DE, Chan MS, Day NP, et al. (2001) Recombination within natural populations of pathogenic bacteria: Short- term empirical estimates and long-term phylogenetic consequences. Proceedings of the National Academy of Sciences USA 98: 182–187.
- 69. Swofford D (1998) PAUP* Phylogenetic analysis using parsimony and other methods. Sunderland, Mass.: Sinauer.
- 70. van Bergen MA, Dingle KE, Maiden MC, Newell DG, van der Graaf-Van Bloois L, et al. (2005) Clonal nature of Campylobacter fetus as defined by multilocus sequence typing. Journal of Clinical Microbiology 43: 5888–5898.
- 71. McVean G, Awadalla P, Fearnhead P (2002) A coalescent-based method for detecting and estimating recombination from gene sequences. Genetics 160: 1231–1241.
- 72. Nielson R, Yang Z (1998) Likelihood models for detecting positively selected amino acid sites and applications to the HIV-1 envelope gene. Genetics 148: 929–936.