Mitigating Phototoxicity during Multiphoton Microscopy of Live Drosophila Embryos in the 1.0–1.2 µm Wavelength Range

Open Access

Peer-reviewed

Research Article

Delphine Débarre ,

* E-mail: delphine.debarre@ujf-grenoble.fr (DD); emmanuel.beaurepaire@polytechnique.edu (EB)

Affiliations Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS UMR 7645, and INSERM U696, Palaiseau, France, Univ. Grenoble Alpes, LIPhy, Grenoble, France, CNRS, LIPhy, Grenoble, France
⨯
Nicolas Olivier ,

¶^¤ Current address: Department of Physics, King's College London, London, United Kingdom

Affiliation Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS UMR 7645, and INSERM U696, Palaiseau, France
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Willy Supatto,

Affiliation Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS UMR 7645, and INSERM U696, Palaiseau, France
⨯
Emmanuel Beaurepaire

* E-mail: delphine.debarre@ujf-grenoble.fr (DD); emmanuel.beaurepaire@polytechnique.edu (EB)

Affiliation Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS UMR 7645, and INSERM U696, Palaiseau, France
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Drosophila melanogaster
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Embryos
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Absorption
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Soil perturbation
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Light
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Fluorescence imaging
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Lasers
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Light pulses
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