Validation of SNP Allele Frequencies Determined by Pooled Next-Generation Sequencing in Natural Populations of a Non-Model Plant Species

Christian Rellstab; Stefan Zoller; Andrew Tedder; Felix Gugerli; Martin C. Fischer

doi:10.1371/journal.pone.0080422

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Open Access

Peer-reviewed

Research Article

Validation of SNP Allele Frequencies Determined by Pooled Next-Generation Sequencing in Natural Populations of a Non-Model Plant Species

Christian Rellstab,

Affiliation Biodiversity and Conservation Biology, Swiss Federal Research Institute WSL, Birmensdorf, Switzerland
⨯
Stefan Zoller,

Affiliation Genetic Diversity Centre, ETH Zürich, Zürich, Switzerland
⨯
Andrew Tedder,

Affiliation Institute of Evolutionary Biology and Environmental Studies and Institute of Plant Biology, University of Zürich, Zürich, Switzerland
⨯
Felix Gugerli,

Affiliation Biodiversity and Conservation Biology, Swiss Federal Research Institute WSL, Birmensdorf, Switzerland
⨯
Martin C. Fischer

* E-mail: martin.fischer@env.ethz.ch

Affiliation Institute of Integrative Biology, ETH Zürich, Zürich, Switzerland
⨯

Validation of SNP Allele Frequencies Determined by Pooled Next-Generation Sequencing in Natural Populations of a Non-Model Plant Species

Christian Rellstab,
Stefan Zoller,
Andrew Tedder,
Felix Gugerli,
Martin C. Fischer

Published: November 7, 2013
https://doi.org/10.1371/journal.pone.0080422

About the Authors

Christian Rellstab

Affiliation Biodiversity and Conservation Biology, Swiss Federal Research Institute WSL, Birmensdorf, Switzerland

Stefan Zoller

Affiliation Genetic Diversity Centre, ETH Zürich, Zürich, Switzerland

Andrew Tedder

Affiliation Institute of Evolutionary Biology and Environmental Studies and Institute of Plant Biology, University of Zürich, Zürich, Switzerland

Felix Gugerli

Affiliation Biodiversity and Conservation Biology, Swiss Federal Research Institute WSL, Birmensdorf, Switzerland

Martin C. Fischer

* E-mail: martin.fischer@env.ethz.ch

Affiliation Institute of Integrative Biology, ETH Zürich, Zürich, Switzerland

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: CR AT FG MCF. Performed the experiments: CR SZ MCF. Analyzed the data: CR SZ AT FG MCF. Contributed reagents/materials/analysis tools: CR SZ AT FG MCF. Wrote the manuscript: CR MCF.

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Single nucleotide polymorphisms
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Polymerase chain reaction
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Arabidopsis thaliana
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Plant genomics
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Genomics
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Linear regression analysis
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Alleles
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Next-generation sequencing
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