Amp-PCR: Combining a Random Unbiased Phi29-Amplification with a Specific Real-Time PCR, Performed in One Tube to Increase PCR Sensitivity

Lena Erlandsson; Lars Peter Nielsen; Anders Fomsgaard

doi:10.1371/journal.pone.0015719

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Open Access

Peer-reviewed

Research Article

Amp-PCR: Combining a Random Unbiased Phi29-Amplification with a Specific Real-Time PCR, Performed in One Tube to Increase PCR Sensitivity

Lena Erlandsson ,

* E-mail: lao@ssi.dk

Affiliation Virus Research and Development, Department of Virology, Statens Serum Institut, Copenhagen, Denmark
⨯
Lars Peter Nielsen,

Affiliation Influenza Laboratory, Department of Virology, Statens Serum Institut, Copenhagen, Denmark
⨯
Anders Fomsgaard

Affiliation Virus Research and Development, Department of Virology, Statens Serum Institut, Copenhagen, Denmark
⨯

Amp-PCR: Combining a Random Unbiased Phi29-Amplification with a Specific Real-Time PCR, Performed in One Tube to Increase PCR Sensitivity

Lena Erlandsson,
Lars Peter Nielsen,
Anders Fomsgaard

Published: December 31, 2010
https://doi.org/10.1371/journal.pone.0015719

About the Authors

Lena Erlandsson

* E-mail: lao@ssi.dk

Affiliation Virus Research and Development, Department of Virology, Statens Serum Institut, Copenhagen, Denmark

Lars Peter Nielsen

Affiliation Influenza Laboratory, Department of Virology, Statens Serum Institut, Copenhagen, Denmark

Anders Fomsgaard

Affiliation Virus Research and Development, Department of Virology, Statens Serum Institut, Copenhagen, Denmark

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: LE LPN AF. Performed the experiments: LE. Analyzed the data: LE. Contributed reagents/materials/analysis tools: LE AF. Wrote the paper: LE LPN AF.

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Polymerase chain reaction
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Signal amplification
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DNA polymerase
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Medical risk factors
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HPV-16
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Waxes
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Circular DNA
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Polymerases
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