DNA Extraction from Dry Museum Beetles without Conferring External Morphological Damage

M. Thomas P. Gilbert; Wendy Moore; Linea Melchior; Michael Worobey

doi:10.1371/journal.pone.0000272

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Open Access

Peer-reviewed

Research Article

DNA Extraction from Dry Museum Beetles without Conferring External Morphological Damage

M. Thomas P. Gilbert ,

*To whom correspondence should be addressed. E-mail: mtpgilbert@gmail.com

Affiliations Department of Ecology and Evolutionary Biology, The University of Arizona, Tucson, Arizona, United States of America, Ancient DNA and Evolution, The Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark
⨯
Wendy Moore,

Affiliation Department of Entomology, California Academy of Sciences, San Francisco, California, United States of America
⨯
Linea Melchior,

Affiliations Department of Ecology and Evolutionary Biology, The University of Arizona, Tucson, Arizona, United States of America, Research Laboratory, Institute of Forensic Medicine, The University of Copenhagen, Copenhagen, Denmark
⨯
Michael Worobey

Affiliation Department of Ecology and Evolutionary Biology, The University of Arizona, Tucson, Arizona, United States of America
⨯

DNA Extraction from Dry Museum Beetles without Conferring External Morphological Damage

M. Thomas P. Gilbert,
Wendy Moore,
Linea Melchior,
Michael Worobey

Published: March 7, 2007
https://doi.org/10.1371/journal.pone.0000272

About the Authors

M. Thomas P. Gilbert

*To whom correspondence should be addressed. E-mail: mtpgilbert@gmail.com

Affiliations Department of Ecology and Evolutionary Biology, The University of Arizona, Tucson, Arizona, United States of America, Ancient DNA and Evolution, The Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark

Wendy Moore

Affiliation Department of Entomology, California Academy of Sciences, San Francisco, California, United States of America

Linea Melchior

Affiliations Department of Ecology and Evolutionary Biology, The University of Arizona, Tucson, Arizona, United States of America, Research Laboratory, Institute of Forensic Medicine, The University of Copenhagen, Copenhagen, Denmark

Michael Worobey

Affiliation Department of Ecology and Evolutionary Biology, The University of Arizona, Tucson, Arizona, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MG WM. Performed the experiments: MW MG WM LM. Analyzed the data: MW MG WM LM. Wrote the paper: MW MG WM LM.

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DNA extraction
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Beetles
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Polymerase chain reaction
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Insects
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Mitochondrial DNA
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DNA annealing
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DNA damage
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Mitochondria
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