October 2022

Saglin is a salivary gland-specific protein that has already been shown to affect transmission of the malaria parasite Plasmodium. The protein is expressed only in the median lobe of the female salivary gland, particularly in the cells of the base, which comprise only about one-third of the lobe. To study the saglin promoter, its coding sequence was replaced with the 3xP3 promoter fused to GFP. The activity of the 3xP3 promoter is usually observed in the larval nervous system, but in some individuals leads to strong GFP expression in the adult ommatidia. Klug et al 2022

Image Credit: Dennis Klug

Opinion

Pestilence and famine: Continuing down the vicious cycle with COVID-19

Sudipta Hyder, Rethy K. Chhem, Filip Claes, Erik Albert Karlsson

PLOS Pathogens: published October 6, 2022 | https://doi.org/10.1371/journal.ppat.1010810

Pearls

Into the wild: How exposure to wild or domesticated fungi shapes immune responses in mice

Ying-Han Chen, Ken Cadwell

PLOS Pathogens: published October 13, 2022 | https://doi.org/10.1371/journal.ppat.1010841

An introduction to the Marburg virus vaccine consortium, MARVAC

Robert W. Cross, Ira M. Longini, Stephan Becker, Karin Bok, David Boucher, Miles W. Carroll, Janet V. Díaz, William E. Dowling, Ruxandra Draghia-Akli, James T. Duworko, John M. Dye, Michael A. Egan, Patricia Fast, Amy Finan, Courtney Finch, Thomas R. Fleming, Joan Fusco, Thomas W. Geisbert, Anthony Griffiths, Stephan Günther, Lisa E. Hensley, Anna Honko, Ruth Hunegnaw, Jocelyn Jakubik, Julie Ledgerwood, Kerstin Luhn, Demetrius Matassov, Jeffrey Meshulam, Emily V. Nelson, Christopher L. Parks, Roxana Rustomjee, David Safronetz, Lauren M. Schwartz, Dean Smith, Paul Smock, Ydrissa Sow, Christina F. Spiropoulou, Nancy J. Sullivan, Kelly L. Warfield, Daniel Wolfe, Courtney Woolsey, Roland Zahn, Ana María Henao-Restrepo, César Muñoz-Fontela, Andrea Marzi

PLOS Pathogens: published October 13, 2022 | https://doi.org/10.1371/journal.ppat.1010805

Current treatments against mucormycosis and future directions

Courtney Smith, Soo Chan Lee

PLOS Pathogens: published October 13, 2022 | https://doi.org/10.1371/journal.ppat.1010858

What bacterial cell death teaches us about life

Alex G. Johnson, Philip J. Kranzusch

PLOS Pathogens: published October 27, 2022 | https://doi.org/10.1371/journal.ppat.1010879

An innate ability: How do basal invertebrates manage their chronic exposure to microbes?

Leah M. Williams, Thomas D. Gilmore

PLOS Pathogens: published October 31, 2022 | https://doi.org/10.1371/journal.ppat.1010897

Review

Not your Mother’s MAPKs: Apicomplexan MAPK function in daughter cell budding

William J. O’Shaughnessy, Pravin S. Dewangan, E. Ariana Paiz, Michael L. Reese

PLOS Pathogens: published October 13, 2022 | https://doi.org/10.1371/journal.ppat.1010849

Research Articles

Type 1 and Type 2 Epstein-Barr viruses induce proliferation, and inhibit differentiation, in infected telomerase-immortalized normal oral keratinocytes

Deo R. Singh, Scott E. Nelson, Abigail S. Pawelski, Juan A. Cantres-Velez, Alisha S. Kansra, Nicholas P. Pauly, Jillian A. Bristol, Mitchell Hayes, Makoto Ohashi, Alejandro Casco, Denis Lee, Stuart A. Fogarty, Paul F. Lambert, Eric C. Johannsen, Shannon C. Kenney

PLOS Pathogens: published October 3, 2022 | https://doi.org/10.1371/journal.ppat.1010868

Human neutrophil IL1β directs intestinal epithelial cell extrusion during Salmonella infection

Anna-Lisa E. Lawrence, Ryan P. Berger, David R. Hill, Sha Huang, Veda K. Yadagiri, Brooke Bons, Courtney Fields, Gautam J. Sule, Jason S. Knight, Christiane E. Wobus, Jason R. Spence, Vincent B. Young, Mary X. O’Riordan, Basel H. Abuaita

PLOS Pathogens: published October 3, 2022 | https://doi.org/10.1371/journal.ppat.1010855

Revisiting Leishmania GP63 host cell targets reveals a limited spectrum of substrates

Marie-Michèle Guay-Vincent, Christine Matte, Anne-Marie Berthiaume, Martin Olivier, Maritza Jaramillo, Albert Descoteaux

PLOS Pathogens: published October 3, 2022 | https://doi.org/10.1371/journal.ppat.1010640

The effect of Omicron breakthrough infection and extended BNT162b2 booster dosing on neutralization breadth against SARS-CoV-2 variants of concern

Carl Graham, Thomas Lechmere, Aisha Rehman, Jeffrey Seow, Ashwini Kurshan, Isabella Huettner, Thomas J. A. Maguire, Jerry C. H. Tam, Daniel Cox, Christopher Ward, Mariusz Racz, Anele Waters, Christine Mant, Michael H. Malim, Julie Fox, Katie J. Doores

PLOS Pathogens: published October 3, 2022 | https://doi.org/10.1371/journal.ppat.1010882

Requirement of a functional ion channel for Sindbis virus glycoprotein transport, CPV-II formation, and efficient virus budding

Zeinab Elmasri, Vashi Negi, Richard J. Kuhn, Joyce Jose

PLOS Pathogens: published October 3, 2022 | https://doi.org/10.1371/journal.ppat.1010892

Modulation of the extracellular matrix by Streptococcus gallolyticus subsp. gallolyticus and importance in cell proliferation

Ritesh Kumar, John Culver Taylor, Antrix Jain, Sung Yun Jung, Victor Garza, Yi Xu

PLOS Pathogens: published October 3, 2022 | https://doi.org/10.1371/journal.ppat.1010894

Enhanced immune responses following heterologous vaccination with self-amplifying RNA and mRNA COVID-19 vaccines

Tamara Elliott, Hannah M. Cheeseman, Abbey B. Evans, Suzanne Day, Leon R. McFarlane, Jessica O’Hara, Mohini Kalyan, Fahimah Amini, Tom Cole, Alan Winston, Sarah Fidler, Katrina M. Pollock, James A. Harker, Robin J. Shattock

PLOS Pathogens: published October 4, 2022 | https://doi.org/10.1371/journal.ppat.1010885

Glycans are not necessary to maintain the pathobiological features of bovine spongiform encephalopathy

Alicia Otero, Tomás Barrio, Hasier Eraña, Jorge M. Charco, Marina Betancor, Carlos M. Díaz-Domínguez, Belén Marín, Olivier Andréoletti, Juan M. Torres, Qingzhong Kong, Juan J. Badiola, Rosa Bolea, Joaquín Castilla

PLOS Pathogens: published October 7, 2022 | https://doi.org/10.1371/journal.ppat.1010900

Immunization with inactivated whole virus particle influenza virus vaccines improves the humoral response landscape in cynomolgus macaques

Brendon Y. Chua, Toshiki Sekiya, Marios Koutsakos, Naoki Nomura, Louise C. Rowntree, Thi H. O. Nguyen, Hayley A. McQuilten, Marumi Ohno, Yuki Ohara, Tomohiro Nishimura, Masafumi Endo, Yasushi Itoh, Jennifer R. Habel, Kevin J. Selva, Adam K. Wheatley, Bruce D. Wines, P. Mark Hogarth, Stephen J. Kent, Amy W. Chung, David C. Jackson, Lorena E. Brown, Masashi Shingai, Katherine Kedzierska, Hiroshi Kida

PLOS Pathogens: published October 7, 2022 | https://doi.org/10.1371/journal.ppat.1010891

miRNA let-7 family regulated by NEAT1 and ARID3A/NF-κB inhibits PRRSV-2 replication in vitro and in vivo

Xiangbin You, Min Liu, Qian Liu, Huijuan Li, Yilin Qu, Xiaoxiao Gao, Chengyu Huang, Gan Luo, Gang Cao, Dequan Xu

PLOS Pathogens: published October 10, 2022 | https://doi.org/10.1371/journal.ppat.1010820

Knockout of signal peptide peptidase in the eye reduces HSV-1 replication and eye disease in ocularly infected mice

Shaohui Wang, Ujjaldeep Jaggi, Homayon Ghiasi

PLOS Pathogens: published October 10, 2022 | https://doi.org/10.1371/journal.ppat.1010898

Inhibition of vaccinia virus L1 N-myristoylation by the host N-myristoyltransferase inhibitor IMP-1088 generates non-infectious virions defective in cell entry

Lalita Priyamvada, Wouter W. Kallemeijn, Monica Faronato, Kimberly Wilkins, Cynthia S. Goldsmith, Catherine A. Cotter, Suany Ojeda, Roberto Solari, Bernard Moss, Edward W. Tate, Panayampalli Subbian Satheshkumar

PLOS Pathogens: published October 10, 2022 | https://doi.org/10.1371/journal.ppat.1010662

Characterization of a multipurpose NS3 surface patch coordinating HCV replicase assembly and virion morphogenesis

Olaf Isken, Minh Tu Pham, Hella Schwanke, Felicia Schlotthauer, Ralf Bartenschlager, Norbert Tautz

PLOS Pathogens: published October 10, 2022 | https://doi.org/10.1371/journal.ppat.1010895

Karyotype variation, spontaneous genome rearrangements affecting chemical insensitivity, and expression level polymorphisms in the plant pathogen Phytophthora infestans revealed using its first chromosome-scale assembly

Michael E. H. Matson, Qihua Liang, Stefano Lonardi, Howard S. Judelson

PLOS Pathogens: published October 10, 2022 | https://doi.org/10.1371/journal.ppat.1010869

A toolbox of engineered mosquito lines to study salivary gland biology and malaria transmission

Dennis Klug, Katharina Arnold, Raquel Mela-Lopez, Eric Marois, Stéphanie A. Blandin

PLOS Pathogens: published October 12, 2022 | https://doi.org/10.1371/journal.ppat.1010881

SERINC5 restricts influenza virus infectivity

Fei Zhao, Fengwen Xu, Xiaoman Liu, Yamei Hu, Liang Wei, Zhangling Fan, Liming Wang, Yu Huang, Shan Mei, Li Guo, Long Yang, Shan Cen, Jianwei Wang, Chen Liang, Fei Guo

PLOS Pathogens: published October 12, 2022 | https://doi.org/10.1371/journal.ppat.1010907

Inhibitors of ApiAP2 protein DNA binding exhibit multistage activity against Plasmodium parasites

Timothy James Russell, Erandi K. De Silva, Valerie M. Crowley, Kathryn Shaw-Saliba, Namita Dube, Gabrielle Josling, Charisse Flerida A. Pasaje, Irene Kouskoumvekaki, Gianni Panagiotou, Jacquin C. Niles, Marcelo Jacobs-Lorena, C. Denise Okafor, Francisco-Javier Gamo, Manuel Llinás

PLOS Pathogens: published October 12, 2022 | https://doi.org/10.1371/journal.ppat.1010887

Glaesserella parasuis serotype 5 breaches the porcine respiratory epithelial barrier by inducing autophagy and blocking the cell membrane Claudin-1 replenishment

Mingxing Liu, Qing Wang, Wenda Wu, Min Chen, Pengyun Zhang, Mengru Guo, Huixing Lin, Zhe Ma, Hong Zhou, Hongjie Fan

PLOS Pathogens: published October 13, 2022 | https://doi.org/10.1371/journal.ppat.1010912

Destruction of the vascular viral receptor in infectious salmon anaemia provides in vivo evidence of homologous attachment interference

Maria Aamelfot, Johanna Hol Fosse, Hildegunn Viljugrein, Frieda Betty Ploss, Sylvie L. Benestad, Alastair McBeath, Debes Hammershaimb Christiansen, Kyle Garver, Knut Falk

PLOS Pathogens: published October 14, 2022 | https://doi.org/10.1371/journal.ppat.1010905

Genetic diversity and evolutionary convergence of cryptic SARS- CoV-2 lineages detected via wastewater sequencing

Devon A. Gregory, Monica Trujillo, Clayton Rushford, Anna Flury, Sherin Kannoly, Kaung Myat San, Dustin T. Lyfoung, Roger W. Wiseman, Karen Bromert, Ming-Yi Zhou, Ellen Kesler, Nathan J. Bivens, Jay Hoskins, Chung-Ho Lin, David H. O’Connor, Chris Wieberg, Jeff Wenzel, Rose S. Kantor, John J. Dennehy, Marc C. Johnson

PLOS Pathogens: published October 14, 2022 | https://doi.org/10.1371/journal.ppat.1010636

TLR2 axis on peripheral blood mononuclear cells regulates inflammatory responses to non-infectious immature dengue virus particles

José Alberto Aguilar Briseño, Lennon Ramos Pereira, Marleen van der Laan, Mindaugas Pauzuolis, Bram M. ter Ellen, Vinit Upasani, Jill Moser, Luís Carlos de Souza Ferreira, Jolanda M. Smit, Izabela A. Rodenhuis-Zybert

PLOS Pathogens: published October 14, 2022 | https://doi.org/10.1371/journal.ppat.1010499

Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome

Liping Wang, Huang Tan, Laura Medina-Puche, Mengshi Wu, Borja Garnelo Gomez, Man Gao, Chaonan Shi, Tamara Jimenez-Gongora, Pengfei Fan, Xue Ding, Dan Zhang, Yi Ding, Tábata Rosas-Díaz, Yujing Liu, Emmanuel Aguilar, Xing Fu, Rosa Lozano-Durán

PLOS Pathogens: published October 18, 2022 | https://doi.org/10.1371/journal.ppat.1010909

Reductions in bacterial viability stimulate the production of Extra-intestinal Pathogenic Escherichia coli (ExPEC) cytoplasm-carrying Extracellular Vesicles (EVs)

Min Jiang, Zhongxing Wang, Fufang Xia, Zhe Wen, Rui Chen, Dongyu Zhu, Min Wang, Xiangkai Zhuge, Jianjun Dai

PLOS Pathogens: published October 19, 2022 | https://doi.org/10.1371/journal.ppat.1010908

Multi-site fungicides suppress banana Panama disease, caused by Fusarium oxysporum f. sp. cubense Tropical Race 4

Stuart Cannon, William Kay, Sreedhar Kilaru, Martin Schuster, Sarah Jane Gurr, Gero Steinberg

PLOS Pathogens: published October 20, 2022 | https://doi.org/10.1371/journal.ppat.1010860

The PD-1/PD-L1 pathway is induced during Borrelia burgdorferi infection and inhibits T cell joint infiltration without compromising bacterial clearance

Jennifer D. Helble, Julie E. McCarthy, Machlan Sawden, Michael N. Starnbach, Linden T. Hu

PLOS Pathogens: published October 20, 2022 | https://doi.org/10.1371/journal.ppat.1010903

A positive feedback loop mediates crosstalk between calcium, cyclic nucleotide and lipid signalling in calcium-induced Toxoplasma gondii egress

Stephanie D. Nofal, Caia Dominicus, Malgorzata Broncel, Nicholas J. Katris, Helen R. Flynn, Gustavo Arrizabalaga, Cyrille Y. Botté, Brandon M. Invergo, Moritz Treeck

PLOS Pathogens: published October 20, 2022 | https://doi.org/10.1371/journal.ppat.1010901

Pathobiology and dysbiosis of the respiratory and intestinal microbiota in 14 months old Golden Syrian hamsters infected with SARS-CoV-2

Brittany Seibert, C. Joaquín Cáceres, Silvia Carnaccini, Stivalis Cardenas-Garcia, L. Claire Gay, Lucia Ortiz, Ginger Geiger, Daniela S. Rajao, Elizabeth Ottesen, Daniel R. Perez

PLOS Pathogens: published October 24, 2022 | https://doi.org/10.1371/journal.ppat.1010734

CD169-mediated restrictive SARS-CoV-2 infection of macrophages induces pro-inflammatory responses

Sallieu Jalloh, Judith Olejnik, Jacob Berrigan, Annuurun Nisa, Ellen L. Suder, Hisashi Akiyama, Maohua Lei, Sita Ramaswamy, Sanjay Tyagi, Yuri Bushkin, Elke Mühlberger, Suryaram Gummuluru

PLOS Pathogens: published October 24, 2022 | https://doi.org/10.1371/journal.ppat.1010479

IL-2–mTORC1 signaling coordinates the STAT1/T-bet axis to ensure Th1 cell differentiation and anti-bacterial immune response in fish

Kete Ai, Kang Li, Xinying Jiao, Yu Zhang, Jiaqi Li, Qian Zhang, Xiumei Wei, Jialong Yang

PLOS Pathogens: published October 25, 2022 | https://doi.org/10.1371/journal.ppat.1010913

Regressive evolution of an effector following a host jump in the Irish potato famine pathogen lineage

Erin K. Zess, Yasin F. Dagdas, Esme Peers, Abbas Maqbool, Mark J. Banfield, Tolga O. Bozkurt, Sophien Kamoun

PLOS Pathogens: published October 27, 2022 | https://doi.org/10.1371/journal.ppat.1010918

A Proximity biotinylation assay with a host protein bait reveals multiple factors modulating enterovirus replication

Seyedehmahsa Moghimi, Ekaterina G. Viktorova, Samuel Gabaglio, Anna Zimina, Bogdan Budnik, Bridge G. Wynn, Elizabeth Sztul, George A. Belov

PLOS Pathogens: published October 28, 2022 | https://doi.org/10.1371/journal.ppat.1010906

Piperaquine-resistant PfCRT mutations differentially impact drug transport, hemoglobin catabolism and parasite physiology in Plasmodium falciparum asexual blood stages

John Okombo, Sachel Mok, Tarrick Qahash, Tomas Yeo, Jade Bath, Lindsey M. Orchard, Edward Owens, Imhoi Koo, Istvan Albert, Manuel Llinás, David A. Fidock

PLOS Pathogens: published October 28, 2022 | https://doi.org/10.1371/journal.ppat.1010926

Dual role of an essential HtrA2/Omi protease in the human malaria parasite: Maintenance of mitochondrial homeostasis and induction of apoptosis-like cell death under cellular stress

Shweta Singh, Gaurav Datta, Shaifali Jain, Vandana Thakur, Priya Arora, Azhar Muneer, Mohd Asad, Shakir Ali, Asif Mohmmed

PLOS Pathogens: published October 28, 2022 | https://doi.org/10.1371/journal.ppat.1010932

Free ISG15 inhibits Pseudorabies virus infection by positively regulating type I IFN signaling

Huimin Liu, Chen Li, Wenfeng He, Jing Chen, Guoqing Yang, Lu Chen, Hongtao Chang

PLOS Pathogens: published October 31, 2022 | https://doi.org/10.1371/journal.ppat.1010921

Correction

Correction: Epstein–Barr virus oncoprotein–driven B cell metabolism remodeling

Eric M. Burton, Benjamin E. Gewurz

PLOS Pathogens: published October 20, 2022 | https://doi.org/10.1371/journal.ppat.1010920

Retraction

Retraction: BRCA1 Regulates IFI16 Mediated Nuclear Innate Sensing of Herpes Viral DNA and Subsequent Induction of the Innate Inflammasome and Interferon-β Responses

The PLOS Pathogens Editors

PLOS Pathogens: published October 19, 2022 | https://doi.org/10.1371/journal.ppat.1010904

Cover

Female sag(-)KI mosquito displaying 3xP3-specific GFP expression in the ommatidia of the compound eyes