Vero cells expressing canine SLAM were co-cultured with white blood cells of ferrets. The animals had been inoculated via different routes with recombinant canine distemper viruses expressing the fluorescent proteins Venus, dTom or TagBFP. Systemic virus dissemination in vivo resulted in circulation of single, double- and triple-infected lymphocytes. Morbillivirus isolation in vitro induced formation of multinucleated syncytia of which the cytoplasm was flooded with different combinations of fluorescent reporter proteins. This virus competition model will facilitate identification of factors associated with viral fitness and transmission. Image taken by confocal laser scanning microscopy. de Swart et al.
Image Credit: Rory de Vries, Paul Duprex and Rik de Swart
Pearls
Leishmania enriettii (Muniz & Medina, 1948): A highly diverse parasite is here to stay
PLOS Pathogens: published May 25, 2017 | https://doi.org/10.1371/journal.ppat.1006303
Targeting pathogen sterols: Defence and counterdefence?
PLOS Pathogens: published May 18, 2017 | https://doi.org/10.1371/journal.ppat.1006297
Candida auris: A rapidly emerging cause of hospital-acquired multidrug-resistant fungal infections globally
PLOS Pathogens: published May 18, 2017 | https://doi.org/10.1371/journal.ppat.1006290
New opportunities and challenges to engineer disease resistance in cassava, a staple food of African small-holder farmers
PLOS Pathogens: published May 11, 2017 | https://doi.org/10.1371/journal.ppat.1006287
Drosophila as a model for homeostatic, antibacterial, and antiviral mechanisms in the gut
PLOS Pathogens: published May 4, 2017 | https://doi.org/10.1371/journal.ppat.1006277
Hijacked: Co-option of host behavior by entomophthoralean fungi
PLOS Pathogens: published May 4, 2017 | https://doi.org/10.1371/journal.ppat.1006274
Research Articles
Structural and functional dissection reveals distinct roles of Ca2+-binding sites in the giant adhesin SiiE of Salmonella enterica
PLOS Pathogens: published May 30, 2017 | https://doi.org/10.1371/journal.ppat.1006418
Hepatitis E virus persists in the presence of a type III interferon response
PLOS Pathogens: published May 30, 2017 | https://doi.org/10.1371/journal.ppat.1006417
MicroRNA 26a (miR-26a)/KLF4 and CREB-C/EBPβ regulate innate immune signaling, the polarization of macrophages and the trafficking of Mycobacterium tuberculosis to lysosomes during infection
PLOS Pathogens: published May 30, 2017 | https://doi.org/10.1371/journal.ppat.1006410
A unique profilin-actin interface is important for malaria parasite motility
PLOS Pathogens: published May 26, 2017 | https://doi.org/10.1371/journal.ppat.1006412
A conformational switch high-throughput screening assay and allosteric inhibition of the flavivirus NS2B-NS3 protease
PLOS Pathogens: published May 25, 2017 | https://doi.org/10.1371/journal.ppat.1006411
Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus
PLOS Pathogens: published May 25, 2017 | https://doi.org/10.1371/journal.ppat.1006387
The murine cytomegalovirus M35 protein antagonizes type I IFN induction downstream of pattern recognition receptors by targeting NF-κB mediated transcription
PLOS Pathogens: published May 25, 2017 | https://doi.org/10.1371/journal.ppat.1006382
Highly efficient maternal-fetal Zika virus transmission in pregnant rhesus macaques
PLOS Pathogens: published May 25, 2017 | https://doi.org/10.1371/journal.ppat.1006378
A spatio-temporal assessment of simian/human immunodeficiency virus (SHIV) evolution reveals a highly dynamic process within the host
PLOS Pathogens: published May 25, 2017 | https://doi.org/10.1371/journal.ppat.1006358
Varicella zoster virus glycoprotein C increases chemokine-mediated leukocyte migration
PLOS Pathogens: published May 25, 2017 | https://doi.org/10.1371/journal.ppat.1006346
Elevated catalase expression in a fungal pathogen is a double-edged sword of iron
PLOS Pathogens: published May 22, 2017 | https://doi.org/10.1371/journal.ppat.1006405
Adaptation of Candida albicans to environmental pH induces cell wall remodelling and enhances innate immune recognition
PLOS Pathogens: published May 22, 2017 | https://doi.org/10.1371/journal.ppat.1006403
Suppression of IL-12p70 formation by IL-2 or following macrophage depletion causes T-cell autoreactivity leading to CNS demyelination in HSV-1-infected mice
PLOS Pathogens: published May 22, 2017 | https://doi.org/10.1371/journal.ppat.1006401
FAS-associated factor-1 positively regulates type I interferon response to RNA virus infection by targeting NLRX1
PLOS Pathogens: published May 22, 2017 | https://doi.org/10.1371/journal.ppat.1006398
Related Articles
Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection
PLOS Pathogens: published May 22, 2017 | https://doi.org/10.1371/journal.ppat.1006397
Mycobacterium tuberculosis arrests host cycle at the G1/S transition to establish long term infection
PLOS Pathogens: published May 22, 2017 | https://doi.org/10.1371/journal.ppat.1006389
Related Articles
Down regulation of macrophage IFNGR1 exacerbates systemic L. monocytogenes infection
PLOS Pathogens: published May 22, 2017 | https://doi.org/10.1371/journal.ppat.1006388
Antifungal Tc17 cells are durable and stable, persisting as long-lasting vaccine memory without plasticity towards IFNγ cells
PLOS Pathogens: published May 22, 2017 | https://doi.org/10.1371/journal.ppat.1006356
Seminal plasma induces inflammation and enhances HIV-1 replication in human cervical tissue explants
PLOS Pathogens: published May 19, 2017 | https://doi.org/10.1371/journal.ppat.1006402
Related Articles
Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue
PLOS Pathogens: published May 19, 2017 | https://doi.org/10.1371/journal.ppat.1006385
Structural basis of nectin-1 recognition by pseudorabies virus glycoprotein D
PLOS Pathogens: published May 19, 2017 | https://doi.org/10.1371/journal.ppat.1006314
Potent and selective inhibition of pathogenic viruses by engineered ubiquitin variants
PLOS Pathogens: published May 18, 2017 | https://doi.org/10.1371/journal.ppat.1006372
Promiscuous signaling by a regulatory system unique to the pandemic PMEN1 pneumococcal lineage
PLOS Pathogens: published May 18, 2017 | https://doi.org/10.1371/journal.ppat.1006339
PknG senses amino acid availability to control metabolism and virulence of Mycobacterium tuberculosis
PLOS Pathogens: published May 17, 2017 | https://doi.org/10.1371/journal.ppat.1006399
Microbiota-induced peritrophic matrix regulates midgut homeostasis and prevents systemic infection of malaria vector mosquitoes
PLOS Pathogens: published May 17, 2017 | https://doi.org/10.1371/journal.ppat.1006391
UGGT1 enhances enterovirus 71 pathogenicity by promoting viral RNA synthesis and viral replication
PLOS Pathogens: published May 17, 2017 | https://doi.org/10.1371/journal.ppat.1006375
Proteomic analysis of Biomphalaria glabrata plasma proteins with binding affinity to those expressed by early developing larval Schistosoma mansoni
PLOS Pathogens: published May 16, 2017 | https://doi.org/10.1371/journal.ppat.1006081
MyD88 signaling in dendritic cells and the intestinal epithelium controls immunity against intestinal infection with C. rodentium
PLOS Pathogens: published May 16, 2017 | https://doi.org/10.1371/journal.ppat.1006357
Structural insights into reptarenavirus cap-snatching machinery
PLOS Pathogens: published May 15, 2017 | https://doi.org/10.1371/journal.ppat.1006400
Compartment-specific distribution of human intestinal innate lymphoid cells is altered in HIV patients under effective therapy
PLOS Pathogens: published May 15, 2017 | https://doi.org/10.1371/journal.ppat.1006373
Systematic, multiparametric analysis of Mycobacterium tuberculosis intracellular infection offers insight into coordinated virulence
PLOS Pathogens: published May 15, 2017 | https://doi.org/10.1371/journal.ppat.1006363
Structure of Ca2+-binding protein-6 from Entamoeba histolytica and its involvement in trophozoite proliferation regulation
PLOS Pathogens: published May 15, 2017 | https://doi.org/10.1371/journal.ppat.1006332
Paradoxical myeloid-derived suppressor cell reduction in the bone marrow of SIV chronically infected macaques
PLOS Pathogens: published May 12, 2017 | https://doi.org/10.1371/journal.ppat.1006395
Characterization of miR-122-independent propagation of HCV
PLOS Pathogens: published May 11, 2017 | https://doi.org/10.1371/journal.ppat.1006374
The host ubiquitin-dependent segregase VCP/p97 is required for the onset of human cytomegalovirus replication
PLOS Pathogens: published May 11, 2017 | https://doi.org/10.1371/journal.ppat.1006329
Mucosal fluid glycoprotein DMBT1 suppresses twitching motility and virulence of the opportunistic pathogen Pseudomonas aeruginosa
PLOS Pathogens: published May 10, 2017 | https://doi.org/10.1371/journal.ppat.1006392
Related Articles
Generational distribution of a Candida glabrata population: Resilient old cells prevail, while younger cells dominate in the vulnerable host
PLOS Pathogens: published May 10, 2017 | https://doi.org/10.1371/journal.ppat.1006355
Delineating morbillivirus entry, dissemination and airborne transmission by studying in vivo competition of multicolor canine distemper viruses in ferrets
PLOS Pathogens: published May 8, 2017 | https://doi.org/10.1371/journal.ppat.1006371
MHC Ib molecule Qa-1 presents Mycobacterium tuberculosis peptide antigens to CD8+ T cells and contributes to protection against infection
PLOS Pathogens: published May 5, 2017 | https://doi.org/10.1371/journal.ppat.1006384
Calmodulin-like proteins localized to the conoid regulate motility and cell invasion by Toxoplasma gondii
PLOS Pathogens: published May 5, 2017 | https://doi.org/10.1371/journal.ppat.1006379
Protein-protein interactions in the RPS4/RRS1 immune receptor complex
PLOS Pathogens: published May 5, 2017 | https://doi.org/10.1371/journal.ppat.1006376
Parallel ClickSeq and Nanopore sequencing elucidates the rapid evolution of defective-interfering RNAs in Flock House virus
PLOS Pathogens: published May 5, 2017 | https://doi.org/10.1371/journal.ppat.1006365
HIV-1 competition experiments in humanized mice show that APOBEC3H imposes selective pressure and promotes virus adaptation
PLOS Pathogens: published May 5, 2017 | https://doi.org/10.1371/journal.ppat.1006348
Related Articles
Genetically-barcoded SIV facilitates enumeration of rebound variants and estimation of reactivation rates in nonhuman primates following interruption of suppressive antiretroviral therapy
PLOS Pathogens: published May 4, 2017 | https://doi.org/10.1371/journal.ppat.1006359
Predicting HIV-1 transmission and antibody neutralization efficacy in vivo from stoichiometric parameters
PLOS Pathogens: published May 4, 2017 | https://doi.org/10.1371/journal.ppat.1006313
A central region in the minor capsid protein of papillomaviruses facilitates viral genome tethering and membrane penetration for mitotic nuclear entry
PLOS Pathogens: published May 2, 2017 | https://doi.org/10.1371/journal.ppat.1006308
Related Articles
Translocation of the papillomavirus L2/vDNA complex across the limiting membrane requires the onset of mitosis
PLOS Pathogens: published May 2, 2017 | https://doi.org/10.1371/journal.ppat.1006200
Related Articles
Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complex
PLOS Pathogens: published May 1, 2017 | https://doi.org/10.1371/journal.ppat.1006370
Controlling transferrin receptor trafficking with GPI-valence in bloodstream stage African trypanosomes
PLOS Pathogens: published May 1, 2017 | https://doi.org/10.1371/journal.ppat.1006366
NLRX1 negatively modulates type I IFN to facilitate KSHV reactivation from latency
PLOS Pathogens: published May 1, 2017 | https://doi.org/10.1371/journal.ppat.1006350