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Rapid deployment of SARS-CoV-2 testing: The CLIAHUB

  • Emily D. Crawford,

    Affiliations Chan Zuckerberg Biohub, San Francisco, California, United States of America, University of California San Francisco, Department of Microbiology and Immunology, San Francisco, California, United States of America

  • Irene Acosta,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Vida Ahyong,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Erika C. Anderson,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Shaun Arevalo,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Daniel Asarnow,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Shannon Axelrod,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Patrick Ayscue,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Camillia S. Azimi,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Caleigh M. Azumaya,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Stefanie Bachl,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Iris Bachmutsky,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Aparna Bhaduri,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Jeremy Bancroft Brown,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Joshua Batson,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Astrid Behnert,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Ryan M. Boileau,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Saumya R. Bollam,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Alain R. Bonny,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • David Booth,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • Michael Jerico B. Borja,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • David Brown,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Bryan Buie,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Cassandra E. Burnett,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Lauren E. Byrnes,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Katelyn A. Cabral,

    Affiliations University of California San Francisco, School of Medicine, San Francisco, California, United States of America, University of California San Francisco, Institute for Neurodegenerative Diseases, San Francisco, California, United States of America

  • Joana P. Cabrera,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Saharai Caldera,

    Affiliations Chan Zuckerberg Biohub, San Francisco, California, United States of America, University of California San Francisco, Division of Infectious Disease, San Francisco, California, United States of America

  • Gabriela Canales,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Gloria R. Castañeda,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Agnes Protacio Chan,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Christopher R. Chang,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Arthur Charles-Orszag,

    Affiliations University of California San Francisco, School of Medicine, San Francisco, California, United States of America, Howard Hughes Medical Institute, Chevy Chase, Maryland, United States of America

  • Carly Cheung,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Unseng Chio,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Eric D. Chow,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • Y. Rose Citron,

    Affiliation University of California, Berkeley, California, United States of America

  • Allison Cohen,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Lillian B. Cohn,

    Affiliations Chan Zuckerberg Biohub, San Francisco, California, United States of America, University of California San Francisco, Department of Experimental Medicine, San Francisco, California, United States of America

  • Charles Chiu,

    Affiliation University of California San Francisco, Department of Laboratory Medicine, San Francisco, California, United States of America

  • Mitchel A. Cole,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Daniel N. Conrad,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Angela Constantino,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Andrew Cote,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Tre’Jon Crayton-Hall,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Spyros Darmanis,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Angela M. Detweiler,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Rebekah L. Dial,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • Shen Dong,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Elias M. Duarte,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • David Dynerman,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Rebecca Egger,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Alison Fanton,

    Affiliation University of California, Berkeley, California, United States of America

  • Stacey M. Frumm,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Becky Xu Hua Fu,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Valentina E. Garcia,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • Julie Garcia,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Christina Gladkova,

    Affiliations University of California San Francisco, School of Medicine, San Francisco, California, United States of America, Howard Hughes Medical Institute, Chevy Chase, Maryland, United States of America

  • Miriam Goldman,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Rafael Gomez-Sjoberg,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • M. Grace Gordon,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • James C. R. Grove,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Shweta Gupta,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Alexis Haddjeri-Hopkins,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Pierce Hadley,

    Affiliations University of California San Francisco, School of Medicine, San Francisco, California, United States of America, University of California San Francisco, Institute for Neurodegenerative Diseases, San Francisco, California, United States of America

  • John Haliburton,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Samantha L. Hao,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • George Hartoularos,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Nadia Herrera,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Melissa Hilberg,

    Affiliation University of California San Francisco, Department of Laboratory Medicine, San Francisco, California, United States of America

  • Kit Ying E. Ho,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Nicholas Hoppe,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Shayan Hosseinzadeh,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Conor J. Howard,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Jeffrey A. Hussmann,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Elizabeth Hwang,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Danielle Ingebrigtsen,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Julia R. Jackson,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Ziad M. Jowhar,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Danielle Kain,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • James Y. S. Kim,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Amy Kistler,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Oriana Kreutzfeld,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Jessie Kulsuptrakul,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Andrew F. Kung,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Charles Langelier,

    Affiliations Chan Zuckerberg Biohub, San Francisco, California, United States of America, University of California San Francisco, Division of Infectious Disease, San Francisco, California, United States of America

  • Matthew T. Laurie,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • Lena Lee,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Kun Leng,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Kristoffer E. Leon,

    Affiliation Gladstone Institute, San Francisco, California, United States of America

  • Manuel D. Leonetti,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Sophia R. Levan,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Sam Li,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • Aileen W. Li,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Jamin Liu,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • Heidi S. Lubin,

    Affiliation eSix Development, Oakland, California, United States of America

  • Amy Lyden,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Jennifer Mann,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Sabrina Mann,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Gorica Margulis,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Diana M. Marquez,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Bryan P. Marsh,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Calla Martyn,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Elizabeth E. McCarthy,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Aaron McGeever,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Alexander F. Merriman,

    Affiliation Gladstone Institute, San Francisco, California, United States of America

  • Lauren K. Meyer,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Steve Miller,

    Affiliation University of California San Francisco, Department of Laboratory Medicine, San Francisco, California, United States of America

  • Megan K. Moore,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Cody T. Mowery,

    Affiliation Gladstone Institute, San Francisco, California, United States of America

  • Tanzila Mukhtar,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Lusajo L. Mwakibete,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Noelle Narez,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Norma F. Neff,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Lindsay A. Osso,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Diter Oviedo,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Suping Peng,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Maira Phelps,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Kiet Phong,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Peter Picard,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Lindsey M. Pieper,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Neha Pincha,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Angela Oliveira Pisco,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Angela Pogson,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Sergei Pourmal,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • Robert R. Puccinelli,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Andreas S. Puschnik,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Elze Rackaityte,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • Preethi Raghavan,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Madhura Raghavan,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • James Reese,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Joseph M. Replogle,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Hanna Retallack,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • Helen Reyes,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Donald Rose,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Marci F. Rosenberg,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Estella Sanchez-Guerrero,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Sydney M. Sattler,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Laura Savy,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Stephanie K. See,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Kristin K. Sellers,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Paula Hayakawa Serpa,

    Affiliations Chan Zuckerberg Biohub, San Francisco, California, United States of America, University of California San Francisco, Division of Infectious Disease, San Francisco, California, United States of America

  • Maureen Sheehy,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Jonathan Sheu,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Sukrit Silas,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Jessica A. Streithorst,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Jack Strickland,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Doug Stryke,

    Affiliation University of California San Francisco, Department of Laboratory Medicine, San Francisco, California, United States of America

  • Sara Sunshine,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • Peter Suslow,

    Affiliation University of California San Francisco, Department of Laboratory Medicine, San Francisco, California, United States of America

  • Renaldo Sutanto,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Serena Tamura,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Michelle Tan,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Jiongyi Tan,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Alice Tang,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Cristina M. Tato,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Jack C. Taylor,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Iliana Tenvooren,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Erin M. Thompson,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Edward C. Thornborrow,

    Affiliation University of California San Francisco, Department of Laboratory Medicine, San Francisco, California, United States of America

  • Eric Tse,

    Affiliation Joint Bioengineering Graduate Program, University of California, Berkeley, California, United States of America

  • Tony Tung,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Marc L. Turner,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Victoria S. Turner,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Rigney E. Turnham,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Mary J. Turocy,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Trisha V. Vaidyanathan,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Ilia D. Vainchtein,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Manu Vanaerschot,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Sara E. Vazquez,

    Affiliation University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

  • Anica M. Wandler,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Anne Wapniarski,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • James T. Webber,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Zara Y. Weinberg,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Alexandra Westbrook,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Allison W. Wong,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Emily Wong,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Gajus Worthington,

    Affiliation Chan Zuckerberg Biohub, San Francisco, California, United States of America

  • Fang Xie,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Albert Xu,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Terrina Yamamoto,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Ying Yang,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Fauna Yarza,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Yefim Zaltsman,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  • Tina Zheng,

    Affiliation University of California San Francisco, School of Medicine, San Francisco, California, United States of America

  •  [ ... ],
  • Joseph L. DeRisi

    joe@derisilab.ucsf.edu

    Affiliations Chan Zuckerberg Biohub, San Francisco, California, United States of America, University of California San Francisco, Department of Biochemistry and Biophysics, San Francisco, California, United States of America

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Rapid deployment of SARS-CoV-2 testing: The CLIAHUB

  • Emily D. Crawford, 
  • Irene Acosta, 
  • Vida Ahyong, 
  • Erika C. Anderson, 
  • Shaun Arevalo, 
  • Daniel Asarnow, 
  • Shannon Axelrod, 
  • Patrick Ayscue, 
  • Camillia S. Azimi, 
  • Caleigh M. Azumaya
PLOS
x

Accurate diagnostic testing is at the center of controlling infectious disease outbreaks. It allows clinicians and public health practitioners to identify and treat infected individuals as well as inform larger public health interventions (such as quarantine) to prevent new infections. The emergence of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)/Coronavirus Disease 2019 (COVID-19) pandemic in the human population in November 2019 has spotlighted the importance of diagnostic testing as a (foundational) bedrock to understand how infections occur and spread. Remarkably, multiple studies now show that asymptomatic and presymptomatic individuals are drivers of community transmission [1]. Thus, broad testing capability is necessary to quell resurgence of outbreaks and reopen the global economy.

Laboratory barriers to testing include the limited availability of space, high-capital infrastructure (robotics and information systems), material resources with uninterrupted supply chains, and trained personnel qualified to conduct assays in Clinical Laboratory Improvement Amendment (CLIA)-approved laboratories. However, sizable untapped capacity exists throughout the United States in a multitude of university research laboratories. Theoretically, this capacity could be directed to rapidly expand emergency response capabilities; however, real barriers exist to their entry into clinical diagnostic testing. Research laboratories—while working with the same suite of methods and from the same biological principles as clinical diagnostic laboratories—have substantively different workflows, expectations of quality control and validation, information management, documentation, traceability, reporting, and regulatory compliance. For research scientists, this burden may appear extremely formidable.

To address the dearth of testing availability in the San Francisco Bay Area, the Chan Zuckerberg Biohub, in partnership with the University of California, San Francisco (UCSF) Clinical Microbiology Lab, rapidly built and deployed an emergency COVID-19 viral testing facility, staffed by a combination of Biohub employees and UCSF graduate students, postdocs, and faculty. These efforts were facilitated by guidance from the Food and Drug Administration (FDA) on February 29, 2020, “Policy for Coronavirus Disease-2019 Tests During the Public Health Emergency” [2], and an Executive Order (March 12, 2020) from California State Governor Gavin Newsom modifying certain California-specific regulatory personnel requirements [3]. The goal of this facility, called the “CLIAHUB,” was to provide free, rapid, high-capacity testing services to local hospitals, Department of Public Health offices, and community-based clinical screening efforts in the San Francisco Bay Area, with a particular emphasis on underserved populations. In this perspective piece, and in the accompanying document [4], we describe in detail the experiences of the Biohub, including our organizational structure, recruitment and training of highly skilled volunteers, automation, information management, engineering, protocol development, and clinical validation. We share the lessons we learned and offer guidance to other research laboratories pursuing rapid expansion of diagnostic capabilities in the context of emergency response for COVID-19 and future pandemic responses.

The Chan Zuckerberg Biohub is a nonprofit research institute that employs approximately 110 people organized into multiple smaller research groups, with nearly half of the staff focused on infectious disease research. The organization’s strong focus on collaborative work, communication, and open science allowed frictionless redistribution of personnel priorities into COVID-19 work. On March 12, 2020, the Biohub’s COVID-19 response staff was organized into 13 work teams (staff members from various research groups and seniority levels were appointed as Team Leads and Backup Team Leads according to their skills and experience). To ensure continuity in the event of an outbreak, laboratory infection control procedures were developed, and all job functions and critical knowledge were held by at least 2 individuals. Communications were facilitated by daily all-hands meetings and dedicated Slack channels. Once clinical testing was underway, volunteers from UCSF were trained to run the assay and organized into 4 nonoverlapping squads to reduce viral transmission risk.

Clinical laboratories are required to meet all applicable federal, state, and local regulations in order to perform patient testing and have to be certified with their state and the Center for Medicare and Medicaid Services (CMS) under CLIA. These regulations specify the laboratory organizational structure and minimum operational standards, with major areas covering the laboratory environment, staffing roles and requirements, information tracking and management, testing validation and procedures, quality assurance, and proficiency testing. Each of these areas had to be addressed with compliance documentation as the CLIAHUB was formed. Ensuring regulatory compliance required close collaboration between the CLIA laboratory director, microbiology section director, and clinical quality assurance and Biohub laboratory lead staff. Since the CLIA laboratory director is ultimately responsible for the operations and quality of test results reported by the laboratory, the importance of this close partnership between the UCSF clinical laboratory and the Biohub cannot be overstated.

The complete CLIAHUB workflow is detailed in Fig 1B and the linked document. Briefly, the CLIAHUB workflow begins with medical courier or shipping company delivered patient samples (nasopharyngeal (NP) or oropharyngeal (OP) swabs). In order to preserve RNA integrity and maximize sensitivity, DNA/RNA Shield was chosen for the transport media. After receipt, patient information was recorded (“accessioned”), and tubes were barcoded, de-swabbed, and queued for transfer into 96 well plates. Transfer from tubes to plates was achieved using both do-it-yourself (“DYI”) engineered and commercial automated solutions. Eight stations using high-speed liquid handlers were used to extract viral RNA, after which reverse transcription polymerase chain reaction (RT-PCR) was run using probes for both the N and E gene of SARS-CoV-2. All reagents and samples were tracked using a custom serverless laboratory information system (LIS). After assay optimization, limit of detection (LOD) was established by serial dilution of a quantified high-titer patient sample, and validation of the assay for the Emergency Use Authorization (EUA) application was achieved by retesting samples previously identified as positive or negative based on a previously approved assay at the UCSF clinical lab.

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Fig 1.

(A) Counties served by CLIAHUB and the growth of testing volume over the first 120 days of operation. The most frequent users of testing at CLIAHUB were the surrounding Bay Area counties; however, outlying areas, such as Placer and Lassen, were also frequent users despite the distance. (B) CLIAHUB testing workflow and approximate timing are shown on normalized to a single sample, for preanalytical (blue), analytical (red), and postanalytical (green/yellow). The time to process a batch of 84 samples was approximately 6.7 h, including preanalytical and postanalytical steps. Median TAT was 24 h, highlighting the variability in extrinsic factors not under laboratory control, such as medical courier delivery times. qPCR, quantitative polymerase chain reaction; TAT, turnaround time.

https://doi.org/10.1371/journal.ppat.1008966.g001

On March 20, 2020, 8 days after starting this project, the CLIAHUB returned its first clinical result. As of this writing (October 17, 2020), the CLIAHUB has returned 151,795 clinical results. A total of 36 California county Department of Public Health (“DPH”), safety net hospitals, jails, skilled nursing facilities, and public clinics have utilized the CLIAHUB for SARS-CoV-2 testing (Fig 1), in addition to community-based studies. In the period from May 8 to June 8, 2020, the CLIAHUB processed over 1,300 samples on average per day. The maximum number of samples processed in a single day was 2,688. Median turnaround time (TAT) was 24 h. For genomic epidemiology, positive samples were routed for full SARS-CoV-2 genome sequencing [1]. While the samples processed by CLIAHUB were heterogeneous and not representative of the general population, several important characteristics of the disease and the test were notable. A broad range of cycle threshold (Ct) values have been observed (from 7 to 45), indicative of a tremendous range of viral loads within this disease. Samples were considered positive if the Ct for both the N and E gene probes were less than 40 for the original standard operating procedure (SOP). If a sample was positive for 1 of the 2 probes, the result was considered indeterminant. In subsequent iterations of the protocol, samples were considered positive if either of the 2 probes were below a Ct of 40. While there was an excellent agreement between both viral probes, in 9% of cases, only 1 of the 2 probes were detected, suggesting that assays that rely on detection of a single gene alone may have a significant false negative rate (Fig 2).

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Fig 2. Concordance between RT-PCR Ct values for the E and N gene probes.

For 91% of samples, agreement between viral gene probe sets were within 5 Ct cycles, with the majority within 2 cycles. For 9% of samples, only 1 gene was detected (5% E gene only, 4% N gene only), leading to an indeterminate result. In all such cases, the sample extraction and RT-PCR was repeated; points plotted represent the retest result. Ct, cycle threshold; ND not detected; RT-PCR, reverse transcription polymerase chain reaction.

https://doi.org/10.1371/journal.ppat.1008966.g002

The largest users of CLIAHUB testing capacity have been Bay Area and outlying county DPHs, including San Mateo, Marin, Alameda, Placer, Tehama, Santa Clara, and Sonoma counties. Feedback from individual counties indicated that access to free and fast testing allowed insight into at-risk populations, which, in turn, led to deployment of medical personnel. In the case of congregate care facilities, intervention and stabilization avoided evacuation of residents to local hospitals. The CLIAHUB also directly enabled community-based studies in the town of Bolinas, the Mission District, and Bayview Hunters Point neighborhoods of San Francisco, and a study of unsheltered persons [1]. These studies provided both clinically actionable results for individuals and communities, in addition to valuable insight about the socioeconomics and demographics of populations that were disproportionately affected. Altogether, the experience of CLIAHUB demonstrates that research facilities can indeed be converted to clinical testing laboratories during a pandemic emergency declaration that are capable of returning high-quality and timely clinical results in order to meet the challenge of a growing pandemic. We hope that the accompanying “How to” document, protocols, and engineering details (dx.doi.org/10.17504/protocols.io.bfi2jkge) will serve as a guide for others in the midst of this or any other future pandemic.

References

  1. 1. Chamie G, Marquez C, Crawford E, Peng J, Petersen M, Schwab D, et al. SARS-CoV-2 Community Transmission disproportionately affects Latinx population during Shelter-in-Place in San Francisco. Clin Infect Dis. 2020. pmid:32821935
  2. 2. Coronavirus (COVID-19) Update: FDA Issues New Policy to Help Expedite Availability of Diagnostics. U.S. Food & Drug Administration; 2020. https://www.fda.gov/news-events/press-announcements/coronavirus-covid-19-update-fda-issues-new-policy-help-expedite-availability-diagnostics
  3. 3. State of California ED. Executive Order N-25-20. https://www.gov.ca.gov/wp-content/uploads/2020/03/3.12.20-EO-N-25-20-COVID-19.pdf
  4. 4. Lyden A, Crawford E, Ahyong V, Vanaerschot M, Hayakawa Serpa P, Raghavan P, et al. CLIAHUB Automated RNA Extraction & RT-PCR Protocol V2. https://www.protocols.io/view/cliahub-automated-rna-extraction-amp-rt-pcr-protoc-bfi2jkge/abstract