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Multi-functional mechanisms of immune evasion by the streptococcal complement inhibitor C5a peptidase

  • Nicola N. Lynskey ,

    Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Supervision, Writing – original draft, Writing – review & editing

    Affiliation Faculty of Medicine, Imperial College London, London, United Kingdom

  • Mark Reglinski,

    Roles Investigation, Methodology, Writing – review & editing

    Affiliation Faculty of Medicine, Imperial College London, London, United Kingdom

  • Damien Calay,

    Roles Resources

    Affiliation Faculty of Medicine, Imperial College London, London, United Kingdom

  • Matthew K. Siggins,

    Roles Investigation

    Affiliation Faculty of Medicine, Imperial College London, London, United Kingdom

  • Justin C. Mason,

    Roles Resources, Writing – review & editing

    Affiliation Faculty of Medicine, Imperial College London, London, United Kingdom

  • Marina Botto,

    Roles Resources, Writing – review & editing

    Affiliation Faculty of Medicine, Imperial College London, London, United Kingdom

  • Shiranee Sriskandan

    Roles Conceptualization, Funding acquisition, Methodology, Supervision, Writing – original draft, Writing – review & editing

    Affiliation Faculty of Medicine, Imperial College London, London, United Kingdom

Multi-functional mechanisms of immune evasion by the streptococcal complement inhibitor C5a peptidase

  • Nicola N. Lynskey, 
  • Mark Reglinski, 
  • Damien Calay, 
  • Matthew K. Siggins, 
  • Justin C. Mason, 
  • Marina Botto, 
  • Shiranee Sriskandan


The complement cascade is crucial for clearance and control of invading pathogens, and as such is a key target for pathogen mediated host modulation. C3 is the central molecule of the complement cascade, and plays a vital role in opsonization of bacteria and recruitment of neutrophils to the site of infection. Streptococcal species have evolved multiple mechanisms to disrupt complement-mediated innate immunity, among which ScpA (C5a peptidase), a C5a inactivating enzyme, is widely conserved. Here we demonstrate for the first time that pyogenic streptococcal species are capable of cleaving C3, and identify C3 and C3a as novel substrates for the streptococcal ScpA, which are functionally inactivated as a result of cleavage 7 amino acids upstream of the natural C3 convertase. Cleavage of C3a by ScpA resulted in disruption of human neutrophil activation, phagocytosis and chemotaxis, while cleavage of C3 generated abnormally-sized C3a and C3b moieties with impaired function, in particular reducing C3 deposition on the bacterial surface. Despite clear effects on human complement, expression of ScpA reduced clearance of group A streptococci in vivo in wildtype and C5 deficient mice, and promoted systemic bacterial dissemination in mice that lacked both C3 and C5, suggesting an additional complement-independent role for ScpA in streptococcal pathogenesis. ScpA was shown to mediate streptococcal adhesion to both human epithelial and endothelial cells, consistent with a role in promoting bacterial invasion within the host. Taken together, these data show that ScpA is a multi-functional virulence factor with both complement-dependent and independent roles in streptococcal pathogenesis.

Author summary

The complement pathway is critical in the innate immune response to bacterial pathogens. It consists of a self-perpetuating proteolytic cascade initiated via three distinct pathways that converge at the central complement protein, C3. Pathogens must evade complement-mediated immunity to cause disease, and inactivation of the C3 protein can dampen all effectors of this pathway. Streptococcal species are the causative agents of an array of infections ranging from the benign to lethal. Using the human pathogen Group A Streptococcus as a representative species, we show that the enzyme ScpA, which is conserved amongst the pyogenic streptococci, cleaves human C3a and also C3, releasing abnormally sized and functionally-impaired fragments. As a result, invading streptococci were less well opsonized and host immune cells not properly activated, reducing bacterial phagocytosis and clearance. Despite manifest in vitro activity against complement factors and human neutrophils, ScpA was still able to contribute to systemic bacterial spread in mice lacking C3 and C5. ScpA was also demonstrated to mediate streptococcal adhesion to both epithelial and endothelial cells, which may enhance bacterial systemic spread. Our study highlights the likely importance of both complement-independent and complement-dependent roles for ScpA in streptococcal pathogenesis.


The complement cascade is crucial for clearance of invading pathogens and thus represents a key target for disruption by such organisms. Individual bacterial species use multiple strategies to escape the complement system, highlighting the importance of this pathway in bacterial immunity [17]. The human pathogen Group A Streptococcus (GAS), the causative agent of over half a million infections globally each year which range from benign to life-threatening, is no exception [8]. The ability of GAS to successfully colonize the host and resist clearance is mediated by an array of virulence factors, a number of which interfere with and inactivate the complement cascade [2,4,911].

The complement pathway comprises a tightly regulated, self-perpetuating proteolytic cascade that results in clearance of pathogens by a combination of opsonization, anaphylatoxin release and formation of the lytic membrane attack complex (MAC). Activation occurs via three routes; classical, alternative and lectin pathways, all of which converge at the central complement component C3 [12], a 186 kDa member of the α-macroglobulin family [13].

C3 is comprised of an α (111 kDa) and β (75 kDa) chain which are linked by multiple disulfide bonds. C3 function is modulated by a sequence of proteolytic events, the first of which is mediated by the C3 convertase, and results in the release of the 9 kDa anaphylatoxin C3a from the N-terminus of the C3 α-chain. Activity of C3a is dependent on the carboxy-terminus of the protein, and is quenched following cleavage of the C-terminal arginine residue by the carboxypeptidase B enzyme [13]. The remaining 177 kDa protein, C3b, comprises the residual 102 kDa α–chain and 75 kDa β–chain, and is the activated form of C3. Conformational changes following cleavage result in exposure of a reactive thioester residue permitting covalent deposition on the bacterial surface. Bound C3b interacts with complement receptors expressed by circulating phagocytes, mediating bacterial uptake and killing.

C3b also binds to the pro-enzyme Factor B, cleavage of which by Factor D results in formation of the enzyme complex C3bBb. C3bBb catalyzes cleavage of C3 to C3b and C3a thus amplifying the complement response. [12] This activity in turn induces formation of the C3b2Bb complex which cleaves the complement component C5. C5 is also a member of the α-macroglobulin family and as such shares a similar structure to C3. C5 is cleaved by C3b2Bb which results in the release of the 10.4 kDa anaphylatoxin C5a from the N-terminus similar to the release of C3a from C3. The remaining, larger C5b protein is necessary for downstream complement activation [13].

While deposition of antibody on the surface of GAS is important in promoting opsonophagocytosis [14], complement deposition plays a pivotal role in the control of GAS infection. A strong selective pressure to resist complement immunity has resulted in the evolution of numerous evasion strategies in GAS [2, 4, 911]. These include expression of virulence factors that bind to and inactivate key components of the complement cascade and/or sequester negative regulators of complement at the bacterial surface. GAS also produces enzymes that cleave and inactivate complement components. These include the promiscuous secreted protease SpeB, which degrades numerous host factors including C3b [4,11], and the C5a-inactivating serine protease ScpA, that is common to many pathogenic streptococci, which specifically cleaves C5a [10,15,16,17].

ScpA is a member of the subtilisin-like serine protease family, containing a highly conserved catalytic triad motif (Asp130, His193, Ser512) that is critical for enzymatic activity [16]. Additionally ScpA has an LPXTG motif at the C-terminus which permits anchoring of the protein to the bacterial cell wall [15]. ScpA is processed from a pre- to pro-peptide by autocatalytic cleavage of the N-terminal 31 amino acids, resulting in a catalytically active protein [10]. The reported substrate for ScpA is the human anaphylatoxin C5a, which plays a key role in neutrophil activation and recruitment to the site of infection. ScpA cleaves C5a at the His67 residue, releasing the C-terminus and rendering the protein inactive [10]. Thus, ScpA activity significantly impedes neutrophil activation and recruitment to the site of infection [17], and has been shown to promote bacterial persistence and dissemination in murine models of infection [1719]. C5a is the only reported substrate for ScpA, which is surprising given the specificity of other members of this enzyme family [20].

Although GAS have been reported to bind to [2, 6, 7, 9] or inactivate [4, 11] numerous effectors of the complement cascade, they have not been reported to cleave the central molecule C3, inactivation of which would dampen all anti-bacterial effectors of this pathway. In a previous proteomic screen we identified a putative uncharacterized protein annotated as a “C3-degrading protease (CppA)” [21] which is conserved amongst several streptococal species [21]. This prompted us to question whether GAS and other pyogenic streptococci could actually specifically cleave the central complement component C3. Here, we report that Groups A, C and G streptococci are able to cleave C3, but that the phenotype was not mediated by CppA. Using GAS as a model organism, we went on to demonstrate that the complement-cleaving activity was mediated by ScpA. As such, we identified C3a and C3 as novel substrates for ScpA, cleavage of which is associated with reduced human neutrophil activation and chemotaxis, and subsequent reduced bacterial opsonophagocytosis and killing.

Expression of ScpA has previously been implicated in streptococcal pathogenesis in murine infection models [1719]. Here, we demonstrate that ScpA confers resistance to bacterial clearance in a streptococcal soft-tissue infection model that was manifest even in mice lacking both C3 and C5. Thus, notwithstanding any inactivation of the human complement cascade, ScpA confers virulence to GAS in mice independently of C3 or C5 cleavage, a phenomenon that, we suggest, is likely to be dependent on ScpA-mediated attachment of GAS to endothelial and epithelial cells, as demonstrated in vitro. Overall, our study characterizes ScpA as a multi-functional, complement inactivating protein, with properties that confer pathogenicity at multiple stages of infection.


Streptococcal species cleave human C3 in a conserved manner

In a previous study we identified a putative “C3-degrading protease” CppA [21], for which homologues exist in other streptococcal species [22], and showed that it is upregulated in GAS lacking a functional version of the regulator RocA [21]. In an effort to determine whether streptococcal species could cleave C3, as has been demonstrated for S. pneumoniae [22], whole cell suspensions of streptococcal species representing Lancefield groups A, C and G were co-incubated with human C3. Human C3 is a 186 kDa protein comprised of a 111 kDa α chain and a 75 kDa β chain [13]. These fragments are linked by multiple disulphide bonds and as such, under reducing conditions, C3 migrates as two separate bands of corresponding size. Incubation of different streptococcal species with C3 generated an additional 100 kDa α-chain (C3αscpA) absent from controls incubated with buffer alone. This demonstrated that the experimental setting did not induce physiological activation of C3 to iC3b, which would result in release of a smaller α-chain, consistent with streptococcal-mediated cleavage of the alpha chain of C3 (Fig 1A).

Fig 1. Streptococcal species cleave human C3.

A) Cleavage of human C3 by representative strains of Groups A (GAS-M1), C (S. zoo and S. equi) and G (GGS) streptococci. Bacterial pellets (4x106 cfu) were incubated with human C3 for 16 hours, 37°C. Specific cleavage of the C3α chain was visualized following SDS-PAGE and resulted in release of a 100 kDa product, C3αscpA (white arrow). No cleavage of the β chain was observed. B) The impact of a panel of protease inhibitors on C3 cleavage by GAS-M1 cell pellets was assessed. GAS-M1 pellets (4x106 cfu) were incubated with human C3 for 16 hours at 37°C in the presence of individual protease inhibitors and compared for ability to generate cleavage fragment C3αscpA (white arrow). Concentration of each protease inhibitor used is indicated. The serine protease Pefabloc was sufficient to inhibit streptococcal cleavage of C3.

Initially we carried out experiments to determine whether the CppA protein, identified in our previous study, was capable of cleaving C3. We were, however, unable to demonstrate any role for CppA in the observed cleavage of human C3, since recombinant CppA did not reproduce the activity of whole streptococcal preparations (S1 and S2A Figs). Furthermore, there was no evidence that the streptococcal cysteine protease SpeB, previously reported to degrade C3b [11], was responsible for cleavage of full-length C3. Whole bacterial cells from isogenic strains that differed only in production of active SpeB (GAS-M49 and GAS-M49ΔscpA) were both equally able to cleave C3, while supernatant fractions of the same strains were unable to cleave full-length C3 at all (S2B Fig).

In order to broadly characterize the type of enzyme mediating C3 cleavage, we tested a panel of 10 protease inhibitors to determine if any were sufficient to impair cleavage. Only the serine protease inhibitor Pefabloc was sufficient to inhibit cleavage of C3 by GAS-M1 (Fig 1B). GAS produce two serine proteases known to interact with the host immune response, the CXC-chemokine-cleaving enzyme SpyCEP [23] and ScpA. Isogenic strains that differed only in production of SpyCEP (GAS-M81 and GAS-M81ΔspyCEP), previously described by our laboratory [24] were equally able to cleave C3, ruling out SpyCEP as the C3-ase (S2C Fig). Thus in order to ascertain whether cleavage of C3 was mediated by ScpA, ScpA-negative isogenic strains were generated using two major GAS serotypes, M1 and M89, and extensively screened to ensure no polar effects on expression of adjacent Mga-regulated, or CovR/S regulated genes had been introduced (S3A–S3F Fig).

ScpA cleaves human C3

Wildtype strains GAS-M1 and GAS-M89 and the corresponding isogenic ScpA deletion mutants were investigated for C3 cleavage activity. As expected, 16 hour incubation of either wildtype strain with C3 yielded the additional 100 kDa C3αscpA fragment consistent with cleavage; importantly, this band was absent following incubation with each of the mutants (Fig 2A). Cleavage of C3 was restored in GAS-M89ΔscpA following complementation with plasmid pOriscpA (Fig 2A). To verify that ScpA alone was mediating cleavage, the activity of recombinantly expressed ScpA (rScpA) (S1 Fig) was also evaluated. Similar to wildtype GAS, incubation of C3 with rScpA resulted in the release of a 100 kDa C3αscpA fragment, confirming that C3 is a novel substrate for ScpA (Fig 2A).

Fig 2. ScpA is necessary and sufficient for cleavage of human C3.

A-C) Cleavage of human complement factors A) C3, B) C3a and C) C5a by whole cell pellets (4x106 cfu) of GAS-M1 vs GAS-M1ΔscpA, GAS-M89 vs GAS-M89ΔscpA and rScpA (100 ng). Only bacterial strains expressing ScpA were able to cleave these complement factors. Cleaved moieties (C3αscpA, C3ascpA, C5ascpA) are indicated by a white arrow in each panel. D) C3αscpA was subjected to N-terminal sequencing. ScpA was demonstrated to cleave C3 between Ala741 and Ser742, 7aa N-terminal to the physiological C3 convertase cleavage site. E) Schematic representation of C3 α and β chains and the internal ScpA cleavage site. C3 is cleaved by C3 convertase to release C3b (grey bar) and C3a (white bar). The vertical black line between them shows the physiological C3 convertase cleavage site. ScpA cleaves C3 at an alternative site (red dotted line, black arrow) to release unique cleavage products, C3ascpA and C3αscpA (red text).

The structural and functional homology shared between C3a and C5a are well reported in the literature [25]. It therefore seemed plausible that the C3a moiety may represent an additional substrate for ScpA, such that cleavage of both the inert N-terminus of full-length C3, comprising C3a, and the activated C3a fragment (released by host C3 convertase from C3) could occur. Incubation of wildtype GAS-M1 and GAS-M89 with human C3a resulted in a clear reduction in size when visualized by SDS-PAGE (Fig 2B), consistent with the size difference observed between the C3α and the C3αscpA fragment of C3 (Fig 2A), and similar to the cleavage pattern observed for C5a (Fig 2C). Disruption of the scpA gene and complementation with plasmid pOriscpA demonstrated that ScpA can cleave the C3a moiety, a finding that was confirmed by use of purified rScpA (Fig 2B). The cleavage of C3a we observed was not fully reversed by disruption of the scpA locus, suggesting that an additional as yet unknown GAS virulence factor may contribute to this effect (Fig 2B). Intriguingly, the ScpA-independent cleavage appeared more pronounced following incubation with GAS-M89ΔscpA compared with GAS-M1ΔscpA, suggesting serotype variation in expression levels.

The pattern of cleavage provided evidence that the C3α-chain was being cleaved at the N terminus within C3a, and thus we carried out N-terminal sequencing of the C3αscpA moiety following 16 hour incubation with rScpA to identify the ScpA cleavage site. The sequence obtained (742-SHLGL-746) indicated that ScpA cleaved C3α seven amino acids N-terminal to the site of physiological C3 convertase (Fig 2D and 2E). The ScpA cleavage site was two amino acids N-terminal to the cleavage site of NalP (S4 Fig), a C3-ase of Neisseria meningitidis that impairs complement-mediated immunity [26]. The disparity in size of ScpA-cleaved C3a (C3ascpA) and C3α (C3αscpA) compared with physiological counterparts C3a and C3b was greater than that reported for NalP, and thus pointed to a likely functional role for ScpA-mediated cleavage on C3 activity. Importantly other complement factors, C1q, C2 and the structurally similar C4, were not cleaved by GAS-M1 under the same conditions (S5 Fig), demonstrating the restricted substrate specificity of this enzyme.

To determine the functional relevance of C3-ase activity of ScpA, cleavage of human complement factors C3, C3a and C5a by rScpA protein were investigated over a time course (Fig 3). Cleavage of C3 by rScpA was tested at a 1:1 molar ratio over four hours, and release of C3αscpA fragment was visualized by SDS-PAGE (Fig 3). Although cleavage of C3 required 30 minutes incubation with rScpA (Fig 3A), complete cleavage of C3a occurred within 30 seconds (Fig 3B). Cleavage of C3a occurred more rapidly than C5a (Fig 3C), suggesting that C3a inactivation may contribute to the phenotype previously attributed to C5a inactivation alone.

Fig 3. Rapid cleavage of complement factors by streptococcal ScpA.

Rate of rScpA cleavage of complement components was assessed at 37°C and visualized by SDS-PAGE and Coomassie staining. A) Cleavage of C3 by rScpA at 1:1 molar ratio. C3αscpA cleavage product (white arrow) was detectable after incubation with rScpA for 30 minutes, and became more pronounced by 60 minutes. B) Cleavage of C3a by rScpA at 10:1 molar ratio. C3a appeared to be completely cleaved to C3ascpA (white arrow) following 0.5 minutes incubation with rScpA. C) Cleavage of C5a by rScpA at 10:1 molar ratio. C5ascpA cleavage product (white arrow) was visible following 0.5 minutes incubation with rScpA. Complete cleavage was observed within 60 minutes.

The staphylococcal cysteine protease Aureolysin (Aur) has previously been reported to cleave C3 when purified from S. aureus supernatants, with a recognized impact on complement function [12]. In order to directly compare rScpA protein function with Aur, we amplified aur from S. aureus strain N315 in order to express and purify rAur (S1 Fig). As described previously, rAur was only able to cleave C3 in HEPES++ reaction buffer [12], so comparative experiments with rScpA were carried out under these conditions rather than THB, as for earlier rScpA cleavage analyses. The rate of C3 cleavage was comparable for rScpA and rAur, and was visible from 30 minutes incubation (S6A Fig).

ScpA cleavage is associated with impaired C3 opsonization of GAS

Cleavage of C3α-chain by ScpA results in the release of a longer C3b-like molecule (C3αscpA) compared with that produced by physiological C3 convertase (Fig 2). We hypothesized that this would impact on protein function, specifically C3 deposition on the bacterial surface, and thus went on to explore this in an experimental setting. Following incubation of live GAS with fresh human serum as a source of complement, the level of C3 deposition on the bacterial surface was quantified by flow cytometry. Initially, experiments were carried out using GAS-M1 and the isogenic GAS-M1ΔscpA strain at a range of serum concentrations. Incubation of GAS with serum at a concentration of 25% or more resulted in the detection of significantly less C3 deposition on the surface of GAS-M1 compared with GAS-M1ΔscpA (Fig 4A). Deposition of C3 fragments on GAS required active complement and was barely detectable when cognate heat-inactivated serum samples were used (Fig 4A), despite similar amounts of C3 being present in the heat-inactivated serum (S7 Fig). Serotype M1 GAS are known to inhibit complement deposition via recruitment of host fibrinogen to the bacterial surface by M1 protein [9]. In order to determine if our findings could be reproduced in GAS serotypes where M protein does not bind fibrinogen [27], we carried out C3 deposition experiments in isogenic M89 strains GAS-M89, GAS-M89ΔscpA, GAS-M89ΔscpApC and GAS-M89ΔpCscpA (Fig 4B). As observed for GAS-M1, loss of expression of ScpA resulted in enhanced C3 deposition on the bacterial surface, which could be reversed following complementation of GAS-M89ΔscpA with ScpA expressed in trans (Fig 4B). These data suggest that ScpA contributes to inhibition of C3 deposition by multiple GAS serotypes and may allow escape from complement-mediated immunity.

Fig 4. ScpA activity impairs C3 deposition on the GAS surface.

A) C3 deposition on GAS-M1 and GAS-M1ΔscpA surface was compared following incubation with 0% to 50% human serum. Deposition of C3 on the bacterial surface was quantified using FITC-conjugated anti-C3. Data represent mean +/- SD of 3 serum donors. C3 deposition was not seen when 50% heat-inactivated (HI) serum was used. B) GAS-M89 and isogenic ΔscpA strains were incubated with 50% human serum for 30 minutes, at 37°C. Deposition of C3 on the bacterial surface was quantified using FITC-conjugated anti-C3. Data represent mean +/- SD of 3–4 technical replicates produced from 3 serum donors. (One-tailed T-test * = p<0.05). In order to combine the percentage of C3-positive bacteria and the binding intensity, C3 deposition is presented as fluorescence index (FI), calculated as the proportion of positive bacteria expressed as a percentage multiplied by the gMFI [52]. C) Visualization of serum-mediated degradation of C3α chain. Degradation of physiological C3bα (right hand panel) and equivalent streptococcal ScpA cleavage product C3αscpA (left hand panel) in serum was assessed over 60 minutes. Purified human C3 or C3b were incubated with rScpA for 16 hours, at 37°C. The relative degradation of resulting C3bα and C3αscpA moieties by 1% C3-depleted serum was visualized by SDS-PAGE. Degradation of both C3αscpA and C3bα was observed. D) The rate of degradation of physiological C3bα and the equivalent streptococcal cleavage product C3αscpA was compared following quantification by densitometry and visualized as a line graph.

The physiological relevance of the reduction in C3 deposition on the bacterial surface as a result of rScpA activity was further assessed by comparison with rAur. Both proteins led to comparable reduction in C3 deposition on the surface of GAS-M1ΔscpA (S6B Fig). To better understand the mechanism by which C3 cleavage by GAS might contribute to a reduction in C3 deposition, we investigated the stability of the abnormally long C3αscpA moiety, compared with regular C3bα-chain. C3αscpA generated by rScpA cleavage was subject to rapid degradation by serum host factors when compared with physiological C3bα chain, indicating reduced stability (Fig 4C and 4D). Together, these data demonstrated that the C3αscpA molecule generated by ScpA cleavage is functionally impaired in its ability to resist degradation by host serum factors and opsonize invading bacteria.

Cleavage of C3 or C3a by ScpA significantly impairs host neutrophil function

We hypothesized that the observed effects of C3 and C3a cleavage would affect bacterial clearance, as cleaved C3a might lack activity and C3bscpA fragments would be less likely to promote enduring phagocytic uptake and killing by the host immune response. ScpA has previously been reported to promote streptococcal resistance to neutrophil-mediated opsonophagocytosis and survival in whole human blood, however the mechanism underlying this was not characterized [17]. While this phenotype could be attributed to loss of neutrophil activation by C5a, we also considered the possibility that the reduction in opsonization of GAS by C3 following cleavage by ScpA might impact on bacterial uptake and clearance as well. Consistent with a central role of ScpA in interference with phagocytic killing, bacterial survival in whole human blood, quantified by Lancefield Assay, was significantly reduced for isogenic mutants no longer expressing ScpA compared with wildtype parent M1 strains (Fig 5A). Importantly the same phenotype was also observed following blockade of CD88 (C5aR1) with the chemical inhibitor PMX205 (Fig 5B) [28], suggesting at least a partial C5-independent role for ScpA in inhibition of bacterial killing and growth in whole human blood. To confirm that neutrophil uptake was affected by ScpA, we compared the ability of purified human neutrophils to phagocytoze GAS-M1 and GAS-M1ΔscpA following opsonization with human serum. Expression of ScpA enhanced bacterial resistance to neutrophil uptake (Fig 5C) and, while we believe the reduction in function of C3αscpA compared with physiological C3bα contributes to this phenotype, we hypothesized that inactivation of C3a and C5a would also impact on neutrophil function.

Fig 5. ScpA activity impairs the host neutrophil response to GAS.

A) Survival of GAS-M1 and GAS-M1ΔscpA in whole human blood was quantified by the classical Lancefield assay to determine resistance to neutrophil-mediated killing. Data represent six individual blood donors (each data point is mean of 4 technical replicates), line denotes median value (Mann Whitney U, ** = p<0.001). B) The role of C5a signaling on GAS survival in whole human blood was quantified by Lancefield assay. Whole blood was pre-treated with C5aR1 inhibitor PMX205 (1 μM) [28] prior to incubation with either GAS-M1 or GAS-M1ΔscpA. Each graph represents an independent donor (mean+/- SD 4 technical replicates, Mann Whitney U, * = p<0.05). C) Neutrophil-mediated uptake of fluorescent GAS-M1 and GAS-M1ΔscpA was quantified following incubation with purified human neutrophils. Each graph represents percentage of neutrophils bearing fluorescent GAS from an independent donor (mean+/- SD 4 technical replicates, Mann Whitney U, * = p<0.05). D) Neutrophil activation was assessed by quantification of surface expression of CD11b on purified neutrophils following incubation with GAS-M1 or GAS-M1ΔscpA. Each graph represents an independent neutrophil donor (mean+/- SD 4 technical replicates, Mann Whitney U, * = p<0.05). E) Quantification of neutrophil migration along a chemokine gradient. Absolute number of purified human neutrophils migrating towards C3a or C5a +/- pre-treatment with rScpA was quantified following 30 minute incubation at 37°C. Each graph represents an independent neutrophil donor (4 technical replicates (mean +/- SD), Mann Whitney U, * = p<0.05). F-G) Neutrophil activation by C3a and C5a +/- pre-treatment with rScpA was quantified in a calcium mobilization assay. Calcium transients in fluo-4-AM labelled neutrophils were elicited by C3a or C5a, and calcium derived MFI was recorded on a FACSCalibur from 0–120 seconds. F) Overall MFI of all neutrophils at 120 seconds were compared. Each graph represents an independent neutrophil donor (4 technical replicates (mean +/- SD), Mann Whitney U, * = p<0.05). G) Calcium flux over 120 seconds was visualized following kinetic analysis. Data presented as a histogram of one donor, representative of 3 donors.

C3a and C5a each display anaphylatoxin and chemoattractive properties, and as such play a key role in the activation and recruitment of neutrophils to the site of infection [25]. Using surface expression of CD11b (CR3) as a marker of activation we observed a significant reduction in neutrophil activation in the presence of ScpA following comparison of wildtype GAS-M1 with isogenic GAS-M1ΔscpA (Fig 5D). This dampening of neutrophil activation suggested a significant loss of function of cleaved C3a and C5a, with potential far-reaching effects on neutrophil-mediated immunity. We hypothesized that neutrophil recruitment via C5a and C3a chemotactic gradients, and activation by calcium flux, would also be impaired. ScpA peptidase activity resulted in a significant reduction in neutrophil migration along both C3a and C5a gradients (Fig 5E). In line with these findings, the cytosolic calcium flux mediated by cleaved C3ascpA and C5ascpA fragments was significantly impaired compared with full-length molecules (Fig 5F and 5G). Importantly, exposure to recombinant ScpA alone did not induce a detectable increase in neutrophil intracellular calcium levels (Fig 5F and 5G). In conclusion, ScpA peptidase activity destroyed the complement chemotactic gradients required for migration of human neutrophils at the local site of infection and significantly impaired functional activation.

C5a independent impact of ScpA in vivo

The structure of complement components is strongly conserved between species so we predicted that the peptidase activity of ScpA would be sufficient to mediate functional cleavage of murine (m) C3, C3a and C5a. Incubation of wildtype GAS-M1 or recombinant ScpA with mC3a and mC5a over 16 hours resulted in a clear reduction in size of both proteins when visualized by SDS-PAGE (S8A and S8B Fig respectively), similar to ScpA-mediated cleavage of human C3a and C5a (Fig 2). Genetic deletion of scpA in isogenic strain GAS-M1ΔscpA removed the ability of GAS to cleave either murine C3a or C5a (S8A and S8B Fig). Importantly however, the rate of cleavage of C3a and C5a was significantly slower than observed for human counterparts (S8C and S8D Fig). A small reduction in C3 deposition on the surface of wildtype GAS-M1 compared with GAS-M1ΔscpA was observed following incubation with fresh murine serum (Fig 6A), demonstrating that ScpA peptidase activity can influence murine C3-opsonization of GAS. We thus went on to determine whether ScpA might play a C5a-independent role in GAS disease outcome in a mouse model.

Fig 6. ScpA enhances streptococcal pathogenesis in wild type and complement deficient mice.

A) C3 deposition on GAS-M1 and GAS-M1ΔscpA surface was compared following incubation with 50% murine serum. Deposition of C3 on the bacterial surface was quantified using FITC-conjugated anti-mouse C3. Data are presented as fluorescence index (FI), calculated as the proportion of positive bacteria expressed as a percentage multiplied by the gMFI (line depicts median of four technical replicates Mann Whitney U, * = p<0.05). B-D) Characterization of GAS-M1 and GAS-M1ΔscpA dissemination from the site of infection (thigh) to the draining inguinal lymph node (LN) in a murine model of soft tissue infection. Comparison of dissemination in B) wildtype C57BL/6 mice (n = 8/group), C) C5-/- mice (C57BL/6 background) (n = 9/group) and D) C3-/-/C5-/- mice (C57BL/6 background) (n = 8/group). Line depicts median value. (Mann Whitney U, No difference (ND) = p>0.05, * = p<0.05). E) Systemic spread of GAS-M1 and GAS-M1ΔscpA in C3-/-/C5-/-mice. Quantitative culture of bacteria recovered from spleen, liver and blood of infected mice.

Soft tissue infections represent an important clinical manifestation of GAS, and can be readily modelled in mice to study the initiation and early events in clearance of infection that were previously implicated in ScpA activity [19]. Accordingly, we utilized a previously characterized model of soft tissue infection to ascertain the role of ScpA in the early stages of dissemination during infection [29], comparing pathogenesis of isogenic GAS-M1 and GAS-M1ΔscpA in wildtype mice. Importantly we could detect ScpA expression at the site of infection in mice infected with GAS-M1, demonstrating the pathophysiological relevance of this virulence factor (S9 Fig). Local spread of GAS-M1 from the site of infection was significantly reduced following genetic deletion of scpA (Fig 6B). Three hours after intra-muscular infection, the bacterial burden at the site of infection was comparable between mice infected with either GAS-M1 strain, however dissemination to the locally draining inguinal lymph node was significantly reduced in mice infected with GAS-M1ΔscpA (Fig 6B). At this early timepoint, systemic spread of GAS-M1 was not detectable. By 24 hours however, any difference between strains was no longer detectable, despite systemic spread to spleen, liver and blood (S10 Fig). To investigate these early pathogenic events, histological sections of thigh were examined from C57Bl/6 mice infected with GAS-M1 or GAS-M1ΔscpA (n = 4/group). At this early, 3 hour time-point, bacteria could only be detected in some sections (GAS-M1: 3/4, M1ΔscpA: 1/4) (S11 Fig); any neutrophil infiltrate was scant and, under high magnification, appeared necrotic in mice infected by either GAS-M1 strain (S11B Fig). Taken together the quantitative data suggested that the early clearance and local containment of infecting GAS was significantly impaired by ScpA, however, we were unable to detect a specific effect on neutrophil recruitment at this time point histologically, despite our results with human cells.

In order to ascertain whether the observed effects of ScpA were independent of any effect on C5 or C5a cleavage, the same experiment was carried out in age and weight-matched C5-/- mice (Fig 6C). Following infection via the intra-muscular route, dissemination of GAS-M1 and GAS-M1ΔscpA was determined by quantitative culture. While no difference was observed at the site of infection, we again found that considerably fewer bacteria were recovered from the draining inguinal lymph node of mice infected with GAS-M1ΔscpA. This suggested that ScpA might be affecting local GAS spread to lymph nodes in a C5a independent manner.

To determine whether these results were indeed due to C3-ase activity of ScpA, we carried out the same experiment in age and weight-matched C3-/-/C5-/- knockout mice that are essentially complement deficient (Fig 6D and 6E). No difference was observed between GAS-M1 and GAS-M1ΔscpA infected groups in clearance from the site of infection to the locally draining lymph node (Fig 6D), suggesting that C3-mediated clearance may be more important than C5 in the lymphatic system. However, surprisingly, expression of ScpA significantly enhanced GAS systemic dissemination to blood, spleen and liver (Fig 6E). These unexpected results pointed to a complement-independent role for ScpA in systemic bacterial clearance in settings where there is no C3 and C5.

ScpA has been implicated as an adhesin for both GAS and GBS, however an adhesin effect in live bacteria interacting with live host cells has not been directly demonstrated [30, 31, 32]. We hypothesized that the enhanced systemic dissemination of GAS-M1 compared with GAS-M1ΔScpA in the absence of complement might be the result of adhesion to and subsequent invasion through endothelial tissue. However, in natural infection, interactions with epithelial cells may be important in initial colonization events. In order to test this hypothesis we went on to characterize the ability of the A549 human lung epithelial cell line and primary human umbilical vein endothelial cells (HUVEC) to support attachment of GAS-M1 and GAS-M89 (Fig 7A+B and 7C+D respectively). After demonstrating that both GAS serotypes could adhere to these cell monolayers, we went on to compare and quantify adherence of isogenic ScpA positive and negative strains. Expression of ScpA enhanced attachment of GAS-M1 and GAS-M89 to A549 (Fig 7A and 7B) and HUVEC (Fig 7C and 7D), but had no effect on passive internalization at this 30 minute time-point (S12 Fig). rScpA protein was also able to bind to fixed HUVEC in a dose-dependent manner (Fig 7E). Interestingly, fixed HUVEC monolayers were able to support greater binding of rScpA in the presence of serum (10% FCS) (Fig 7E), an effect which was also seen for attachment of GAS-M1, but not GAS-M1ΔScpA to live HUVEC monolayers (Fig 7C). Expression of ScpA was also sufficient to promote GAS-M1 adhesion to murine cardiac endothelial cells (MCEC-1) (Fig 7F), suggesting that this phenotype could explain the enhanced virulence displayed by GAS-M1 in the murine model (Fig 6). These data support a role for ScpA as an adhesin for attachment of live GAS to epithelial and endothelial cells, which may contribute to the invasive potential of this bacterium in vivo.

Fig 7. ScpA requires serum factors to mediate GAS adhesion to epithelial and endothelial cells.

A+B) Adhesion of A) GAS-M1 and B) GAS-M89 and isogenic ΔscpA strains to A549 epithelial cells was compared by quantitative culture (30 min incubation). Data represent mean+/-SD of 3 experimental replicates (T-test * < 0.05). C+D) Adhesion of C) GAS-M1 and D) GAS-M89 and isogenic ΔscpA strains to primary human HUVEC was compared by quantitative culture (30 min incubation). Data represent mean+/-SD of 3 experimental replicates (T-test * < 0.05, ** < 0.001). E) Adherence of rScpA protein to paraformaldehyde-fixed human HUVEC. Bound rScpA was quantified following incubation with anti-ScpA mouse serum and goat-anti mouse HRP antibody and detection with TMB. Adhesion was compared in the presence and absence of 10% FCS. Data represent mean+/-SD of 3 experimental replicates (T-test * < 0.05). F) Adhesion of GAS-M1 and GAS-M1ΔscpA to the murine endothelial cell line MCEC-1 was compared by quantitative culture (30 min incubation). Data represent mean+/-SD of 3 experimental replicates (T-test * < 0.05).

In order to better define the role of ScpA during infection, we generated strain GAS-M89ΔpCcat2 which over-expresses enzymatically inactive ScpA due to a mutation in the second amino acid of the catalytic triad His193 to alanine [16]. The catalytically inactive ScpA included an additional SNP not predicted to affect structure or expression. We went on to confirm that this mutant was no longer able to cleave C3a (Fig 8A) or C5a (Fig 8B), in contrast to plasmid-transformed GAS-M89ΔpCscpA that over-expressed intact ScpA, consistent with previously reported ScpA structure function studies [16]. To ascertain whether the observed ScpA-enhanced dissemination of GAS (Fig 6) was dependent on catalytic activity, we compared the virulence of strains GAS-M89ΔpC, GAS-M89ΔpCscpA and GAS-M89ΔpCcat2 during soft-tissue infections in age and weight-matched C57BL/6 mice.

Fig 8. Catalytic activity of ScpA is not required for pathogenesis in vivo.

A+B) Cleavage of human complement factors A) C3a and B) C5a by whole cell pellets (4x106 cfu) of GAS-M89ΔpC, GAS-M89ΔpCscpA and GAS-M89ΔpCcat2. Only strain GAS-M89ΔpCscpA, expressing catalytically active ScpA was able to cleave either complement factor. Cleaved moieties (C3ascpA and C5ascpA) are indicated by a white arrow in each panel. C+D) Characterization of GAS-M89ΔpC, GAS-M89ΔpCscpA and GAS-M89ΔpCcat2 dissemination in a murine model of soft tissue infection in wildtype CD57/BL6 mice (n = 5/group). Quantitative culture of tissue homogenates (C) from the site of infection (thigh) to (D) the draining inguinal lymph node (LN). Line depicts median value. (Mann Whitney U, No difference (ns) = p>0.05, * = p<0.05).

Following infection via the intra-muscular route, bacterial dissemination was determined by quantitative culture. As we found previously for GAS-M1 and GAS-M1ΔScpA (Fig 6B), no difference was observed in bacterial counts recovered from the site of infection (Fig 8C). Intriguingly, greater numbers of GAS were recovered from the draining inguinal lymph node of mice infected with GAS expressing ScpA, regardless of whether the ScpA was catalytically active (Fig 8D). This demonstrated that the properties of ScpA that were critical for pathogenesis in the mouse model were independent of any enzymatic activity, pointing to a potentially important role for adhesion in disease progression through interactions with endothelial or epithelial cells.

It was not possible to deduce the relative contribution of ScpA adhesion to pathogenesis in a human system, albeit that ScpA conferred adhesion to human and murine cells. The discrepancy in rates of ScpA-mediated cleavage of human and murine complement factors (Fig 3, S8A and S8B Fig) and the smaller reduction in murine C3 (Fig 6A) compared with human C3 (Fig 4A) deposited on the GAS-M1 surface raised the possibility that, in human disease, the impact of complement cleavage may be of greater importance than adhesion. Our study highlights the multi-functional mechanisms by which ScpA is able to promote bacterial invasion, through a combination of factors including adhesion and active inactivation of the innate immune response.


Streptococcal C5a peptidase is expressed by a variety of streptococcal species. It thus is believed to play a pivotal role in the success of the streptococci, however to date its role in immune evasion has largely been attributed to its ability to cleave C5a. In this work we have identified multifunctional effects of ScpA which include rapid cleavage and inactivation of the anaphylatoxin C3a as well as cleavage of the central complement protein C3. In addition, we have demonstrated complement-independent activity of ScpA as an adhesin, that may contribute to systemic spread during infection in vivo. The complement cascade plays a crucial role in the innate immune response to invading bacteria and as such represents a key target for pathogen immune evasion [1,3,4,10,22,33,34]. The inactivation of both anaphylatoxins C3a and C5a as well as the central complement component C3 we report here has the potential not only to reduce inflammation and cellular activation and recruitment at the site of infection, but also to dampen amplification of the complement system in response to infection, a phenomenon that has not previously been reported for the Group A Streptococci.

ScpA is expressed by all GAS serotypes, and homologous enzymes are encoded by multiple other Lancefield groups [3538]. Conservation of enzyme function both within and between species points to a clear functional evolutionary pressure to systematically disrupt neutrophil responses [36], despite ScpA being a primary target for opsonizing or neutralizing antibodies produced during infection [3843]. ScpA is a member of the subtilisin-like family of serine proteases which are reported to cleave multiple enzymatic substrates, and, while the streptococcal protease SpyCEP cleaves numerous ELR+ CXC chemokines [24], C5a was hitherto the only reported substrate for ScpA.

Our investigation into C3 cleavage by streptococci began following the identification of a putative “C3 degrading protease” (CppA) [21] although no such activity could be ascribed to CppA. Initial analyses established that groups A, C and G streptococci were, however, capable of specifically cleaving full-length C3, a function that has not previously been reported for these species. Furthermore, cleavage activity could be inhibited by the serine protease inhibitor Pefabloc, which led us to hypothesize that ScpA could be mediating C3 cleavage, plausible given the structural similarity between C5a and C3a [25]. The cleavage of C3 that we observe begins within 30 minutes incubation with rScpA protein. Whilst we believe this to be physiologically relevant during infection, it may be that large numbers of bacteria, such as at the site of infection, are required for ScpA C3-ase activity to play a significant role in pathogenesis.

The C3 molecule is an obvious target for pathogen immune evasion, and, as such, other bacterial species also express inactivating enzymes. Cleavage of C3 generating a longer C3bscpA and a shorter C3ascpA molecule by GAS reproduces the mechanism described for the N. meningitidis C3-ase NalP [26]. The reported C3-cleaving enzymes of other Gram positive pathogens, Staphylococcus aureus (aureolysin) [12] and Enterococcus faecalis (GelE) [44], which both cleave 2 amino acids upstream of the physiological C3 convertase site, generate a smaller C3b and longer C3a moiety, in contrast to our findings for ScpA. Cleavage of C3a is thus a function uniquely shared by ScpA and NalP [26], however any effect of such cleavage on C3a activity has not previously been reported.

The GAS cysteine protease SpeB has previously been reported to degrade C3b in human serum, where ScpA could not [11]. In our study, we examined full-length C3, which we demonstrate could be cleaved by ScpA, but not SpeB. This suggests that the various C3-ase functions of these enzymes are distinct, yet may act synergistically to overcome the host innate immune response. C3 is present in human serum at approximately 1 mg/ml, therefore multiple strategies for effective inactivation may be necessary to overcome this highly abundant molecule. Although ScpA is known to be regulated by Mga, our preliminary experiments using available isogenic strains deficient in functional CovR/S or RocA (Regulator of Cov), showed that ScpA was also a member of the CovR regulon (S13 Fig). Both SpeB and ScpA are regulated by CovR/S, however they display reciprocal expression profiles. It is therefore possible that each protein acts at a different phase of infection to modulate the host immune response. SpeB expression is significantly reduced in naturally occurring CovR/S mutants, which are frequently associated with invasive infection. As such, ScpA, which is expressed by invasive and non-invasive disease isolates, may represent a common mechanism of action shared amongst GAS isolates.

The significance of ScpA as a standalone virulence factor was assessed by the generation of isogenic mutants in two GAS serotypes. Loss of function had a significant effect on C3 deposition and stability, and on neutrophil activation by the anaphylatoxins C3a and C5a. Indeed, cleavage of these two proteins reduced neutrophil recruitment along chemotactic gradients and impaired activation, specifically upregulation of CD11b and induction of intracellular calcium release which normally play an important role in pathogen phagocytosis. Fibrinogen binding by the M1 protein is known to have a major influence on GAS-M1 clearance and may render other virulence factors redundant [9]. Nonetheless, ScpA had a measurable effect on C3 deposition on GAS-M89 (where fibrinogen binding is not thought to be dominant) [27] and also in whole blood assays that contain human fibrinogen, both in the presence and absence of a C5aR1 antagonist, suggesting that the C5-independent activities we describe are detectable even in the presence of fibrinogen.

In order to determine the functional relevance of the novel C3 and C3a cleavage activity, in vivo experiments were initially performed in both wildtype and C5-/-mice. Interestingly, similar results were obtained in both genetic backgrounds, suggesting that ScpA plays a role in streptococcal pathogenesis that is independent of C5a cleavage. Importantly, cleavage of C3 not only impairs C3a and C3b function, but also reduces generation of C3b and C5a. Generation of C3b via the alternative pathway is amplified by activity of the C3 convertase, which is comprised of multiple subunits including C3b. Reduction in activity of the alternative pathway C3 convertase will subsequently reduce activation of terminal complement, one output of which is functional C5a. Moreover, C3b is essential for formation of the C5 convertase, and, as such, inactivation of C3 will significantly impair C5a generation and reduce the activity of the entire complement cascade [13]. Thus, in combination with other pan-streptococcal cell envelope serine proteases such as SpyCEP, which cleaves the potent chemoattractant IL-8/CXCL8 and other ELR+ CXC chemokines [23], GAS is capable of systematic inhibition of neutrophil migration and activation, possibly explaining the dearth of functional neutrophils recruited to the site of infection during severe clinical invasive disease.

To validate a clear role for the C3-ase activity of ScpA in vivo, utilizing the same soft-tissue infection model, we compared the pathogenesis of GAS-M1 and GAS-M1ΔscpA in complement deficient (C3-/-/C5-/-) mice. The difference in clearance of bacteria from the site of infection to the locally draining inguinal lymph node observed in both wildtype and C5 deficient mice was diminished in C3-/-/C5-/- mice, suggesting that cleavage of C3 may be responsible for this phenotype. However, systemic dissemination via the blood was significantly impaired in the absence of ScpA, suggesting an additional complement-independent role for this protein in augmenting systemic spread.

Using isogenic GAS strains and recombinant protein, we were able to show that ScpA is a potent adhesin, important for bacterial attachment to both human epithelial and endothelial cells. We went on to demonstrate that ScpA could also mediate attachment of GAS-M1 to murine endothelial cells, providing support for the hypothesis that adhesion was playing a key role in GAS pathogenesis in our in vivo model. Using bacteria expressing ScpA constructs that differed only in catalytic activity, we found that the enzymatic activity of ScpA was not necessary for pathogenicity in a murine model of soft tissue infection, noting the presence of an additional mutation in the enzymatically inactive construct that is not predicted to affect structure or expression. While extrapolating the relevance of our findings to human infection is beyond the scope of this study, the data strongly implicate complement-independent functions of ScpA in streptococcal virulence and disease progression in human infection. We speculate that the failure to control GAS infection in humans can be attributed in part to the complement-independent ability of ScpA to act as an adhesin for endothelial and other cell types, which may play a key role in infection where complement is absent.

Here we have identified complement factors C3 and C3a as two novel substrates of the streptococcal enzyme C5a peptidase and demonstrated that the cleavage of these factors has significant functional effects on the host immune system, most explicitly on human neutrophil activation and function. We have also characterized the role of ScpA as an adhesin, important for attachment to epithelial and endothelial cells. The multiple functions of ScpA described demonstrate its fundamental role in streptococcal disease, and underline the importance of assigning novel functions to known virulence factors as a means to provide new insight into disease progression and pathogenesis.


Ethics statement

Human blood from anonymized consenting healthy donors was obtained from an approved subcollection of the Imperial College NHS Trust Tissue Bank (ICHTB reference R12023). In vivo experiments were performed in accordance with the Animals (Scientific Procedures) Act 1986, and were approved by the Imperial College Ethical Review Process (ERP) panel and the UK Home Office (Project Licence 70/7379).

Bacterial strains

The strains used for molecular manipulation were invasive disease isolates GAS-M1 (H598) [14] and GAS-M89 (H293) [45]. All strains used are listed in Table 1. GAS were cultured on Columbia horse blood agar plates (OXOID) Todd-Hewitt (TH) agar or in TH broth (OXOID) at 37°C, 5% CO2 for 16 hours. E. coli XL-10 gold (Stratagene), TOP10 (ThermoFisher Scientific) BL21(DE3) (ThermoFisher Scientific) were grown in LB broth. Growth media were supplemented with antibiotics where appropriate at the following concentrations; E. coli erythromycin 500 μg/ml, kanamycin 50 μg/ml, ampicillin 100 μg/ml; GAS erythromycin 1 μg/ml, kanamycin 400 μg/ml.

Polymerase chain reaction and DNA sequencing

Genomic DNA was extracted from GAS cultures grown to late logarithmic growth phase (OD600 0.7–0.9) as described previously [21]. PCR was carried out using a MyCycler (Bio-Rad) thermal cycler with Bio-X-Act proof reading Taq (Bioline). Automated-fluorescent sequencing of PCR products and plasmids was performed by the MRC CSC Core Genomics Laboratory, Hammersmith Hospital.

Construction of scpA disruption mutant

Deletion of scpA in GAS-M1 from nucleotide 333–1777 and GAS-M89 from nucleotide 333–2017, resulting in removal of the characterized catalytic domain (S3 Fig). A 500 bp fragment of the scpA gene was amplified (GAS-M1: forward primer: 5’- GGAATTCGCTTTAATAATCGTCCATGG -3’, reverse primer: 5’- GGGGTACCCCTCAAGCAAGGTTCACCTG -3’; GAS-M89: forward primer: 5’- GGAATTCTTCGATATCCTCTATGTTTTC -3’, reverse primer: 5’- GGGGTACCGAGTTGTATTACCAAGCAAC -3’) incorporating EcoRI and KpnI restriction sites into the 5’ and 3’ ends respectively, and cloned into the suicide vector pUCMUT to produce pUCMUTGAS-M1 and pUCMUTGAS-M89. A 3’ 500 bp fragment of the scpA gene was amplified (GAS-M1: forward primer: 5’- ACGCGTCGACGACCTGAGAAGGGTCGTTC -3’, reverse primer: 5’- AACTGCAGCTGTATCATATGCAAATAACC -3’; GAS-M89: forward primer: 5’- ACGCGTCGACCTGAGAAGGGTCGTTCAAATC -3’, reverse primer: 5’- AACTGCAGCTGTATCATATGCAAATAACC -3’) incorporating PstI and SalI restriction sites into the 5’ and 3’ ends respectively, and cloned into PstI/SalI digested pUCMUTGAS-M1 and pUCMUTGAS-M89 respectively. The constructs were introduced into GAS-M1 and GAS-M89 by electroporation and incorporated chromosomally by homologous recombination as described previously [21]. PCR analysis demonstrated that only a single recombination event had occurred. For complementation scpA was cloned into replicative vector pOri23 (scpApOri F: 5’- CCGGATCCCATCAGGAAAGGACGACACATTGC-3’, scpApOri R: 5’- CCGGATCCGATCAGTTGTACTAATCTTCAGTGC-3’) incorporating in BamHI sites at both 5’ and 3’ termini. pOriscpA was propagated in One Shot® TOP10 Chemically Competent E. coli (Invitrogen) and transformed into electrocompetent GAS as described previously [21]. For generation of a complementation vector expressing catalytically inactive ScpA, the second amino acid of the catalytic triad His193, was mutated to alanine in vector pOriScpA by site directed mutagenesis (QuikChange XL-II Site-Directed Mutagenesis Kit, Stratagene) (forward primer: 5′- GCTGTCGATCAAGAGGCCGGCACACACGTGTC -3′, reverse primer: 5′- GACACGTGTGTGCCGGCCTCTTGATCGACAGC -3′) to produce vector pOriscpAcat2. Vector pOriscpAcat2 was sequenced and the desired mutation at residue 193 (His-Ala) confirmed. An additional non-synonymous SNP at position 243 (Ala to Thr) was not deemed to be relevant as it was not within any domains predicted to mediate adhesion. Full-length ScpA protein of size ~120 kDa was confirmed by western blotting and equivalent to that produced by pOriscpA. The vector was transformed into electrocompetent GAS-M89ΔscpA as described previously [21].

Recombinant protein expression

Recombinant proteins were produced using the following primer sequences (CppA: cppA_pET-F: 5’- CCGGATCCAATGACTTTAATGGAAAATATTAC -3’ cppA_pET-R: 5’ CCGGATCCTTCATTTCGTAAACCATACTTC 3’, HVR: emm_pET-F: 5’- CCGGATCCAGGTTTTGCGAATCAAACAGAGGTTAAG -3’, emm_pET-R: 5’- CCGGATCCTAGCTCTCTTAAAATCTCTTCCTGCAACTTCC -3’, Aur: aur_pET-F: 5’- CGGGATCCGATTGATTCAAAAAATAAACC -3’ aur_pET-R: 5’- CGGGATCCTTACTCCACGCCTACTTCATTC) and the pET-19b overexpression system as previously described [49]. rCppA, rEmm 1.0 hypervariable region (HVR), rAur and the previously described rScpA fragment [49], truncated at amino acid 720 to prevent pro-peptide removal, were purified using the Ni-NTA purification system (Invitrogen) according to the manufacturer's instructions. Purified proteins were buffer-exchanged into PBS. Full-length rScpA was expressed following amplification from gDNA (scpA_pET-F: 5’- CCGGATCCAACCAAAACCCCACAAACTC-3’, scpA_pET-R: 5’- CCGGATCCTAGAGTGGCCCTCCAATAGC-3’), and purified from BL21 cell lysates by size exclusion chromatography using a 1 × 50 cm Econo-Column® (Bio-Rad) packed with Sephadex G-100 (Pharmacia). PBS fractions containing ScpA were identified by SDS-PAGE, pooled and concentrated using a 30,000 MWCO spin column (Vivaspin, GE Healthcare).

Cleavage of complement factors and visualization by SDS-PAGE

16 hour timepoint: Recombinant ScpA (100 ng) or stationary phase GAS pellets (4x106 cfu) washed twice in sterile PBS and concentrated 4x, were incubated with C3 (1 μg) (MD Millipore), C3a (0.5 μg) (R&D) or C5a (0.5 μg) (R&D) in THB in a final volume 10 μl. Protease inhibitor kit (Roche) was used, and each inhibitor used at concentrations described previously [23]. All samples were incubated for 16 hours, 37°C, 5% CO2. Pellets were centrifuged and resulting supernatants analyzed. Timecourse: 0.5 μM rScpA or rAur was incubated with C3 (0.5 μM) (MD Millipore), C3a (5 mM) (R&D) or C5a (5mM) (R&D) in THB or HEPES++ buffer (20 mM HEPES, 140 mM NaCl, 5 mM CaCl2, 2.5 mM MgCl2). Samples were fractionated on 3–8% Tris-acetate gels (C3 and C3b) or 4–12% Bis-Tris plus gels (C3a and C5a) (ThermoFisher Scientific) and stained with Instant Blue (Expedeon).

N-terminal sequencing

To determine the ScpA cleavage site, 8 μg purified C3 (EMD Millipore) were cleaved with recombinant ScpA in THB for 16 hours, 37°C. Following cleavage, fragments were subjected to SDS-PAGE and transferred onto Hybond-LFP (Amersham). Membranes were stained with Amido Black (Sigma) and destained (25% v/v isopropanol, 10% v/v acetic acid). Cleaved C3αscpA fragment was excised and subjected to sequencing by Edman degradation (AltaBioscience, Birmingham UK).


Primary antibodies: For western blot, chicken anti-C3 (Abcam ab14232) (1:10,000), goat anti-his (Invitrogen) (1:50,000), rabbit anti-SIC [14] and rabbit anti-SpyCEP [24] serum (1:1000). Mouse antisera against Emm 1.0 HVR and the truncated ScpA molecule were raised by vaccination as described previously [49] and used at 1:1000 dilution. Secondary antibodies used were HRP-conjugated goat anti-chicken IgY, anti-mouse IgG and anti-rabbit IgG (Abcam) (all used at 1:80,000). For C3 deposition, FITC conjugated goat anti-human C3 and goat anti-mouse C3 antibodies (MP Biomedicals) were used (1:300).

Preparation of streptococcal cell wall extracts/supernatants and immunoblotting

Cell wall extracts and bacterial supernatants were prepared as described previously [50] with minor modifications. Briefly, stationary phase cultures were pelleted and supernatants were 0.2 μm filtered. Cell pellets were incubated in cell wall extraction buffer (30% raffinose, 1 μg/ml lysozyme, 10 mM Tris-HCl (pH 8)) at 37°C, 3 hours and cell wall fraction was isolated by centrifugation. Raffinose was removed following dialysis into PBS using Slide-A-lyzer cassettes (ThermoFisher Scientific), and concentrated to 100 μg/ml. 1 μg cell wall extract was loaded for each sample to allow direct comparison between strains. Samples were separated on 4–12% Bis-Tris plus gels (ThermoFisher Scientific) and transferred onto Hybond-LFP membrane (Amersham). Membranes were blocked (5% skimmed milk powder (Sigma), 0.05% Tween (Sigma) and probed and detected with relevant primary and secondary antibodies. Membranes were developed using ECL Advance western blotting detection system (GE Healthcare).

Degradation of C3bαscpA fragment

Following cleavage of C3 with recombinant ScpA, the resulting fragments, or purified C3b (MD Millipore) were incubated with 1% C3-depleted human serum (MD Millpore) for 60 minutes. Samples were taken at 5, 10, 15, 30 and 60 minute timepoints. The reaction was stopped following addition of LDS sample buffer (ThermoFisher Scientific) and 0.1mM DTT, and degradation visualized by western blot using chicken anti-C3 (Abcam).

Capsule quantification

GAS strains were cultured in THB and capsule quantified as described previously [21] using the hyaluronan DuoSet (R&D).

C3 deposition assay

Stationary phase cultures of GAS (1x107 cfu) were washed and resuspended in fresh human serum (0–50%) or mouse serum (50%) and incubated for 30 minutes, 37°C with end-over-end rotation. Control samples were incubated with fresh heat-inactivated human serum and human or mouse C3-depleted serum (EMD Millipore). Cells were stained with FITC-conjugated anti-C3 antibody (MP Biomedicals) for 30 minutes on ice [51] and analyzed using a FACSCalibur cell analyzer (BD Biosciences) and FlowJo Software (Treestar, OR). In order to combine the percentage of C3-positive bacteria and the C3-binding intensity, the results are presented as fluorescence index (FI), which is calculated as the proportion of positive bacteria expressed as a percentage multiplied by the gMFI) [52].

Human whole blood phagocytosis assay

Lancefield assays were performed to assess GAS resistance to human phagocytic killing. GAS were cultured to OD600 0.15 in THB, and diluted in sterile PBS. Approximately 50 GAS cfu were inoculated into heparinized whole human blood obtained from healthy volunteers, and incubated for 3 hours at 37°C with end-over-end rotation. Quantitative culture at this time point was carried out to determine the number of surviving bacteria. To ascertain the role of ScpA-mediated C5a cleavage, C5a receptors were blocked by pre-treatment of whole blood with 1 μM PMX205 [28], 37°C, 30 minutes, end-over-end rotation. Bacterial survival was quantified as multiplication factor of number of surviving colonies relative to the starting inoculum. Each strain was cultured in blood from at least three donors and tested in triplicate.

Neutrophil purification

Neutrophils were purified from fresh human blood using the MACSxpress® Neutrophil Isolation Kit (Miltenyi Biotec) according to manufacturer’s guidelines. Red cells were water-lysed and neutrophils used immediately. All cytometry was carried out on a FACSCalibur cell analyzer (BD Biosciences) and analyzed using FlowJo Software (Treestar, OR).

Neutrophil phagocytosis assays

For neutrophil uptake studies, GAS were pre-labelled with FITC-conjugated group A carbohydrate antibody (Abcam) and uptake by purified human neutrophils was assayed as described previously [29]. Breifly, GAS were opsonized with fresh human serum (+/- pre-treatment with 0.5 μM rScpA or rAur for 30 minutes at 37°C with end-over-end rotation). Opsonophagocytosis was carried out following incubation of 5x105 neutrophils with opsonized bacteria for 30 minutes at 37°C with end-over-end rotation. The reaction was stopped by addition of stop solution (0.02% EDTA in 0.9% saline) and phagocytosis was quantified as percentage of FITC-positive neutrophils, indicative of bacterial uptake. For surface staining, 5x105 fresh neutrophils were incubated with unlabeled GAS for 30 minutes, 37°C with end-to-end rotation and blocked with Human TruStain FcXTM (Biolegend) for 10 minutes on ice. Surface staining for CD11b was carried out using AF488-conjugated anti-CD11-b (Biolegend 301318) or isotype control antibody (Biolegend 400129) to assess non-specific binding (S1 Table) for 20 minutes on ice, and gMFI ascertained for all samples.

Neutrophil chemotaxis assay

24-well transwell plates (Corning 3421) were blocked with RPMI (10% FCS) for 30 minutes prior to addition of rScpA-cleaved and uncleaved C3a or C5a (R&D) (+/- pre-treatment with 100 ng rScpA, 37°C, 16 hours). 1x106 purified neutrophils were added to the inner chamber, and incubated for 30 minutes, 37°C, 5% CO2. The number of neutrophils which had migrated into the lower chamber were quantified using CountBright™ Absolute Counting Beads (ThermoFisher Scientific).

Calcium mobilization assay

A flow cytometric method for calcium mobilization was carried out as described previously [53] with minor modifications. Briefly, neutrophils (1x107/ml) were labeled with fluo-4-AM (0.9 μM) in assay buffer (103 mM NaCl, 4.6 mM KCl, 1 mM CaCl2, 5 mM glucose, 20 mM HEPES, pH7.4). rScpA-cleaved (100 ng) and uncleaved C3a or C5a (100 ng/ml) (R&D) were added directly to neutrophils, and change in mean fluorescence intensity was measured immediately and recorded over 120 seconds.

Murine intra-muscular infection

6–8 week old C57BL/6 (Charles River, Margate, UK), and age and weight-matched C57BL/6 C5-/- and C57BL/6 C3-/-/C5-/- female mice were challenged intra-muscularly with 1×108 GAS, and quantitative endpoints compared at 3 hours post infection. Mice were euthanized, blood taken by cardiac puncture and infected thigh muscle, spleen, liver, and draining inguinal lymph nodes dissected. All organs were plated to quantify bacterial cfu and systemic dissemination. Additional C57BL/6 mice were challenged intra-muscularly with 1×108 GAS. Whole thighs were formalin-fixed, and paraffin-embedded for histopathology imaging as described previously [24], or homogenized and prepared for western blotting as described above. No animals were excluded from the study and randomization was not necessary as mice were genetically identical and age, weight and sex matched. No blinding was carried out as mice were caged separately to prevent contamination with different GAS strains.

Cell culture

The human lung epithelial cell line A549 was cultured in RPMI supplemented with 10% FCS, L-glutamine (2 mM), 37°C, 5% CO2. Primary human umbilical vein endothelial cells (HUVEC) were obtained from fresh human umbilical cords by collagenase treatment as previously reported [54]. Monolayers were cultured in Medium 199 supplemented with 20% FCS, Heparin (10 U/ml), endothelial cell growth supplement (30 μg/ml, Millipore), L-glutamine (2 mM), Pen/strep (1X) 37°C, 5% CO2. Murine cardiac endothelial cell line (MCEC-1) [55] was cultured in DMEM supplemented with 20% FCS, Heparin (10 U/ml), endothelial cell growth supplement (30 μg/ml, Millipore), L-glutamine (2 mM), Pen/strep (1X) at 37°C with 5% CO2. Human and murine endothelial cells were cultured in flasks or plates coated with 1% gelatin (Sigma).

GAS adhesion assays

A549 and HUVEC cells were seeded into 24-well plates and cultured to confluence. GAS were cultured in THB and washed and resuspended in PBS before being inoculated into each well at an MOI of 10. Adhesion assays were carried out following incubation for 30 minutes at 37°C, 5% CO2. Cells were washed 3 times with 1x HBSS (Gibco) to remove non-adherent bacteria and cell-associated bacteria were released following lysis of the cell monolayer in sterile water with trypsin-EDTA (Gibco) and quantified by serial dilution and plating onto CBA. To ensure any differences observed in bacterial recovery were not due to variation in cellular internalization, replica experimental wells were set up. Instead of cell lysis, monolayers were treated with gentamicin (100 μg/ml) in RPMI+FCS+L-glut for 2 hours, 37°C, 5% CO2. Cells were subsequently washed and lysed as described above and internalized bacteria were quantified as for adhesion assays.

rScpA binding to HUVEC

HUVEC were cultured to confluence in 96-well plates and fixed in paraformaldehyde (2%), 15 minutes, room temperature. Cells were washed 3 times in PBS and blocked in 1% BSA, 1 hour, room temperature. rScpA was added to cells in PBS or PBS-FCS (10%), in doubling dilutions from 250 ng. Bound protein was detected following incubation with mouse anti-ScpA serum (1:1000) for 2 hours at room temperature and goat-anti mouse-HRP (1:1000) (Abcam) for 1 hour, room temperature. Samples were incubated with 3, 3', 5, 5'-Tetramethylbenzidine (TMB) substrate (Sigma) for 20 minutes, room temperature and reaction stopped following addition of 1 M H2S04. Relative levels of rScpA binding were quantified as A450 values.


All statistical analyses were performed with GraphPad Prism 5.0. Comparison of two datasets was carried out using unpaired Mann-Whitney U or T-test. A p-value of ≤0.05 was considered significant.

Supporting information

S1 Fig. Expression of recombinant proteins CppA, ScpA and Aur.

SDS-PAGE of recombinantly expressed proteins CppA, ScpA, and Aur, demonstrating the purity of preparations. All proteins were eluted or buffer-exchanged into PBS. For CppA and ScpA gels, molecular weight marker lane has been spliced together with relevant recombinant protein lanes as marked.


S2 Fig. Specific cleavage of human C3 is not mediated by streptococcal proteins CppA, SPEB, or SpyCEP.

Attempted cleavage of human C3 by A) rCppA protein, B) Cell pellets (4x106 cfu) and supernatants from isogenic strains GAS-M49 and GAS-M49ΔspeB, and C) Cell pellets (4x106 cfu) of isogenic GAS-M81 and GAS-M81ΔspyCEP, following co-incubation for 16 hours, 37°C. For panel B, horizontal line visible in right panel (cell pellets) is lined paper in background.


S3 Fig. Generation of GAS M1 and M89 isogenic ScpA knock-out strains.

A) Genetic inactivation of ScpA was achieved by allelic exchange mutagenesis using suicide vector pUCMUT. Double recombination events between R1 and R2 resulted in replacement of a 1500 bp region of scpA containing the putative peptidase domain, including catalytic triad, with kanamycin resistance gene aphA3 (1500 bp). The cloning strategy was designed such that the size of the resulting mga operon transcript would be the same as for the wildtype strain to reduce the risk of polar effects on mga. The orientation of aphA3 and its promoter is opposite to that of the mga operon to prevent alteration of transcript levels of adjacent genes. B-F) Characterization of ScpA allelic exchange mutants; Comparative growth curves, quantified as optical density at 600 nm, for B) GAS-M1 and GAS-M1ΔscpA and C) GAS-M89, GAS-M89ΔscpA, and GAS-M89ΔscpA subsequently complemented with either empty replicative vector, pOri (GAS-M89ΔpC) or with replicative vector pOri expressing ScpA (GAS-M89ΔpCscpA). Error bars represent mean+/- SD of 3 biological replicates. D) Visualization of ScpA protein expression by GAS-M89, GAS-M89ΔscpA, GAS-M89ΔpC and GAS-M89ΔpCscpA by western blot. ScpA protein in 1 μg bacterial cell wall extract was compared between strains following detection with anti-ScpA mouse serum. E) Assessment of mutagenesis-induced polar effects on the mga operon. Comparison between GAS-M1 and GAS-M1ΔscpA for effects on expression of fba by qPCR (data represent mean +/- SD fold change calculated by ΔΔct method) and ScpA, SIC, and M protein by western blot. F) Assessment of mutagenesis-induced effects on global regulatory system CovR/S. To rule out polar effects on the CovR/S regulon, expression of two virulence factors directly regulated by CovR, SpyCEP and the hyaluronic acid capsule, were compared by western blot of cell wall extract and ELISA respectively. (Capsule: mean +/- SD of 4 experimental replicates, ns = p > 0.05).


S4 Fig. Physiological and bacterial C3 cleavage sites.

Schematic representation of C3 cleavage site by physiological C3 convertase (top panel), and by bacterial C3-ases NalP (Neisseria meningitidis) (second panel), Aureolysin (Staphylococcus aureus) (third panel), and ScpA (GAS) (bottom panel).


S5 Fig. C1q, C2 and C4 were not cleaved by ScpA.

GAS-M1 cell pellets (4x106 cfu) or buffer alone (THB) were incubated with each complement factor at 37°C for 16 hours. SDS-PAGE of supernatant following centrifugation was assessed and demonstrated that only complement factor C3 was cleaved by GAS-M1 (cleavage product indicated by black box). Protein chains observed were as expected for all complement factors under denaturing conditions.


S6 Fig. Cleavage of C3 by rScpA occurs at a comparable rate to rAur.

A) Rate of cleavage of C3 protein by rScpA and rAur over a 4 hour period following incubation of proteins at 1:1 molar ratio in HEPES++ buffer. C3αscpA and C3αaur cleavage products (white arrows) both became detectable after 30 minutes incubation, and became more pronounced after 60 minutes. Duration of incubation in hours is indicated at top of each lane. B) C3 deposition on GAS-M1ΔscpA was compared following incubation with fresh human serum which had been pre-incubated with 0.5 μM of either rScpA or rAur. Data are presented as fluorescence index (FI), calculated as the proportion of positive bacteria expressed as a percentage multiplied by the gMFI. Data represent mean +/- SD of 4 technical replicates, (one-tailed T-test * = p < 0.05).


S7 Fig. C3 protein is not degraded in human serum following heat-inactivation.

Fresh human serum was heat-inactivated (HI) by incubation at 56°C for 30 minutes. Degradation of C3 in 1% heat-inactivated serum was compared with 1% fresh serum from the same donor, and C3-depleted serum, and assessed by western blot analysis. Expected C3 α and β chains were observed for fresh and heat-inactivated serum under denaturing conditions, demonstrating C3 was intact following heat-inactivation.


S8 Fig. ScpA cleaves murine complement factors.

A-B) Cleavage of murine complement factors A) mC3a and B) mC5a by whole cell pellets of GAS-M1 vs GAS-M1ΔscpA and rScpA (100 ng). Only GAS-M1 expressing ScpA was able to cleave these complement factors. Cleaved moieties (C3ascpA, C5ascpA) are indicated by a white arrow in each panel. C-D) Rate of rScpA cleavage of complement components was assessed over 60 minutes at 37°C and visualized by SDS-PAGE and Coomassie staining. C) Cleavage of mC3a by rScpA at 10:1 molar ratio. D) Cleavage of mC5a by rScpA at 10:1 molar ratio. Duration of incubation in minutes is indicated above each lane.


S9 Fig. ScpA is expressed during murine intra-muscular infection.

Expression analysis of ScpA in vivo. C57BL/6 mice were infected intra-muscularly with GAS-M1 or GAS-M1ΔscpA (n = 4/group). ScpA expression in 10 μg thigh tissue homogenate was compared between individual mice following immunoblot with anti-ScpA mouse serum. The band unique to GAS-M1 infected mice at 110 kDa is ScpA.


S10 Fig. Streptococcal dissemination in vivo at 24 hours.

Characterization of GAS-M1 and GAS-M1ΔscpA dissemination from the site of infection in a murine model of soft tissue infection in wildtype C57BL/6 mice (n = 8/group) at 24 hour timepoint. Mice were infected intra-muscularly in the thigh. A) Bacteria at the site of infection, B) local spread to the draining inguinal lymph node, and subsequent systemic dissemination to C) spleen, D) liver and E) blood were assessed by quantitative culture. Each data point indicates one mouse and line depicts median value. (Mann Whitney U, * = p<0.05).


S11 Fig. Early pathological changes at the site of infection in a murine model.

Haemotoxylin and eosin stained tissue sections of whole thigh obtained from C57BL/6 mice 3 hours after intra-muscular infection with GAS-M1 or GAS-M1ΔscpA (n = 4/group). A) Magnification x10. White arrows indicate regions with detectable GAS. Contiguous sections were Gram stained to aid detection of bacteria. B) Higher power (40x) images of sections with detectable bacteria from mice infected with GAS-M1 (n = 3/4) and GAS-M1ΔscpA (n = 1/4). White arrows indicate regions with detectable GAS. Note very limited but necrotic leukocyte infiltrate.


S12 Fig. ScpA does not mediate internalization of GAS by epithelial or endothelial cells.

A+B) Internalization of A) GAS-M1 and B) GAS-M89 and isogenic ΔscpA strains by A549 epithelial cells was compared by quantitative culture (30 min incubation). Data represent mean+/-SD of 3 experimental replicates. C+D) Internalization of C) GAS-M1 and D) GAS-M89 and isogenic ΔscpA strains by primary human HUVEC was compared by quantitative culture (30 min incubation). Data represent mean+/-SD of 3 experimental replicates.


S13 Fig. ScpA is a member of the CovR and RocA regulon.

Western blot comparing expression of ScpA between pairs of isogenic strains expressing active or inactive CovR or RocA (strains listed in Table 1). ScpA expression in 1 μg bacterial cell wall extract was compared between isogenic strains following detection with anti-ScpA mouse serum. Functional CovR and RocA repressed expression of ScpA.


S1 Table. Flow cytometry CD11b antibody specificity (4 technical replicates, representative of 3 donors).



The authors would like to thank Professor Andreas Podbielski for providing streptococcal strains GAS-M49 and GAS-M49ΔSpeB, and Professor Andrew Waller for providing S. equi and S. zooepidemicus isolates. We also thank Marokh Nohadani for undertaking histopathological preparation of infected tissues. The authors also acknowledge the Imperial College NIHR BRC Tissue Bank.


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