Group B Streptococcus (GBS), a common commensal of the female genital tract, is the leading cause of invasive infections in neonates. Expression of major GBS virulence factors, such as the hemolysin operon cyl, is regulated directly at the transcriptional level by the CovSR two-component system. Using a random genetic approach, we identified a multi-spanning transmembrane protein, Abx1, essential for the production of the GBS hemolysin. Despite its similarity to eukaryotic CaaX proteases, the Abx1 function is not involved in a post-translational modification of the GBS hemolysin. Instead, we demonstrate that Abx1 regulates transcription of several virulence genes, including those comprising the hemolysin operon, by a CovSR-dependent mechanism. By combining genetic analyses, transcriptome profiling, and site-directed mutagenesis, we showed that Abx1 is a regulator of the histidine kinase CovS. Overexpression of Abx1 is sufficient to activate virulence gene expression through CovS, overcoming the need for an additional signal. Conversely, the absence of Abx1 has the opposite effect on virulence gene expression consistent with CovS locked in a kinase-competent state. Using a bacterial two-hybrid system, direct interaction between Abx1 and CovS was mapped specifically to CovS domains involved in signal processing. We demonstrate that the CovSR two-component system is the core of a signaling pathway integrating the regulation of CovS by Abx1 in addition to the regulation of CovR by the serine/threonine kinase Stk1. In conclusion, our study reports a regulatory function for Abx1, a member of a large protein family with a characteristic Abi-domain, which forms a signaling complex with the histidine kinase CovS in GBS.
The gram-positive Streptococcus genus includes three major human pathogens that are members of the normal microflora: Streptococcus pneumoniae (also known as the pneumococcus), Streptococcus pyogenes (Group A Streptococcus), and Streptococcus agalactiae (Group B Streptococcus). Their carriage in the population is highly dynamic and mostly asymptomatic. However, each of these species can cause a wide spectrum of diseases, from local infections to systemic and fatal infections including septicemia and meningitis. Expression of streptococcal virulence-associated genes is tightly regulated at the transcriptional level. However, the signal(s) and the precise molecular events controlling the switch from commensalism to virulence are not yet understood. In this study, we identified and characterized a bacterial protein essential for virulence gene expression in Group B Streptococcus, the main pathogen of neonates. We show that this transmembrane protein, named Abx1, interacts with the histidine kinase CovS to modulate the activity of the major regulator of virulence CovR. We define how a core set of four proteins, Abx1, CovS, CovR, and the serine/threonine kinase Stk1, interact to control the expression of virulence genes in S. agalactiae. We propose that Abx1-like proteins, that are widespread in bacteria, might be part of a conserved mechanism of two-component system regulation.
Citation: Firon A, Tazi A, Da Cunha V, Brinster S, Sauvage E, Dramsi S, et al. (2013) The Abi-domain Protein Abx1 Interacts with the CovS Histidine Kinase to Control Virulence Gene Expression in Group B Streptococcus. PLoS Pathog 9(2): e1003179. https://doi.org/10.1371/journal.ppat.1003179
Editor: Robin Charles May, University of Birmingham, United Kingdom
Received: September 6, 2012; Accepted: December 19, 2012; Published: February 21, 2013
Copyright: © 2013 Firon et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grants from the National Institute of Health (NIH R01 AI052455-06A1 to DTG and PTC), the Agence Nationale de la Recherche (ANR-10-BLAN-1321 FattyBact to CP and PTC, and Grant ANR-08-StrepRespire to CP), and by financial supports from the Pasteur Institute (to PTC), CNRS (to CP, PG, and PTC), INSERM and Université Paris Descartes (to CP). SB was the recipient of a postdoctoral fellowship from the Region Ile-de-France (DIM MalInf). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Some commensal microorganisms are also opportunistic pathogens. Harmless, and potentially beneficial, they may become causative agents of local or systemic infections , . To date, the signals dictating the switch from commensalism to virulence are mainly unknown. The complex set of genetic and environmental factors thought to be involved would affect the equilibrium between the host and the microbes. Deciphering the molecular events that govern the transition between commensalism and virulence will contribute to understanding and controlling infections due to opportunistic pathogens.
Streptococcus agalactiae (Group B Streptococcus, GBS) is a commensal bacterium of the adult gastro-intestinal tract and is present asymptomatically in the vaginal flora of 10–30% of healthy women . However, GBS is the leading cause of invasive infections in neonates (pneumonia, septicaemia, and meningitis) and a serious cause of mortality or morbidity in adults with underlying diseases –. As with most streptococci, the ability of GBS to cause infections is multifactorial . The main virulence-associated GBS proteins identified to date are secreted or surface components, including the ß-hemolysin/cytolysin and specific adhesins –.
Expression of several major GBS virulence genes is regulated at the transcriptional level by a two-component system (TCS) called CovSR (Control of virulence Sensor and Regulator; also known as CsrSR) –. TCS is the main signaling mechanism used by bacteria to respond to their changing environments . CovSR controls the GBS response to acid stress in vitro and is necessary in vivo at several steps of the infectious process, such as resistance to macrophage killing and penetration of the blood-brain barrier –. Depending on the model of infection, inactivation of the CovSR system leads to a decrease or an increase of virulence –, , suggesting that this system is tightly regulated in space and time to specifically adapt the bacterial virulence capacities to the infected compartments of the host. CovSR orthologs are present in several streptococcal species, including Streptococcus pyogenes (Group A Streptococcus, GAS), where their function as a master regulator of virulence gene expression is conserved –.
The CovSR system belongs to the EnvZ/OmpR family of TCS, where CovS is the membrane-bound histidine protein kinase (HK) and CovR is the cytosolic response regulator (RR). Typically, HK is the sensor of an environmental stimulus that induces its autophosphorylation and, subsequently, phosphorylation of its cognate RR . Phosphorylation of the RR alters its function, generally by modifying its binding affinity for target promoter regions and thereby changing expression of specific genes or operons. In contrast to the majority of TCS, the streptococcal CovSR regulatory pathway is a combination of two antagonistic negative regulators . Indeed, in both GBS and GAS, the phosphorylated CovR acts mainly as a transcriptional inhibitor by direct binding to target gene promoters , , –. It was therefore suggested, but not formally demonstrated in vitro, that CovS acts mainly as a phosphatase on CovR to de-repress virulence gene expression during infection , , . Currently, it is not known whether CovS is activated by general stresses ,  and/or is a direct sensor of specific ligands . The latter hypothesis is supported in GAS by the identification of extracellular Mg2+ and sub-inhibitory concentrations of LL-37, an antimicrobial peptide secreted by innate immune cells, as specific ligands that respectively activate and inhibit CovS activity , , .
Additional levels of TCS regulation exist that do not depend directly on environmental signal sensing by the HK sensor –. These cellular regulators target either the HK or the RR and were named “third components”, “auxiliary proteins”, “adaptors” or “TCS connectors”. While keeping the specific molecular links between an HK and its cognate RR, the additional cellular regulator allows to coordinate the cellular response and/or to integrate multiple signals . This is the case in GBS where CovR activity is modulated by a second signaling pathway mediated by the serine/threonine kinase Stk1 , , . Direct phosphorylation of the CovR T65 threonyl residue by Stk1 decreases CovR activity and interferes negatively with CovS-dependent phosphorylation of the D53 aspartyl residue . These CovS- and Stk1- signaling pathways converge on CovR and are both necessary for GBS virulence .
In this study, we identified and characterized a transmembrane protein, Abx1, belonging to a large family of bacterial proteins of unknown function that have a conserved domain called Abi , . We provide evidence that the putative protease activity of Abx1, inferred from its similarity to eukaryotic CaaX proteases , , is not necessary for its function. Instead, we demonstrate that Abx1 is an additional partner of the CovSR system that is necessary to regulate CovS activity by a protein-protein interaction. We show that the Abx1-CovS signaling complex and the Stk1-dependent pathway are both necessary to control CovR activity. In addition to defining the genetic network controlling virulence gene expression in GBS, we provide the first report of a cellular regulator of HK activity belonging to the large family of bacterial Abi-domain proteins.
Gbs1532/Abx1 is required for pigment production and hemolysis in GBS
The ß-hemolysin/cytolysin expressed by GBS is an important virulence factor encoded within a cluster of twelve genes forming the cyl operon , , . Hemolytic activity of GBS is always associated with the synthesis of an orange pigment. Among the 12 genes of the cyl operon (gbs0644-gbs0655 genes), the CylE protein (gbs0651) is the critical determinant for the dual hemolytic/pigmentation phenotypes , –. Inactivation of cylE abolishes hemolytic activity and pigmentation, and the mutant is less virulent in animal models of systemic infections –.
Screening of a collection of random Himar1 insertional mutants derived from the NEM316 wild-type (WT) strain allowed us to identify mutants that mapped in the cyl operon . We extended this approach with approx. 2,500 new Himar1 mutants screened for hemolytic activity on blood agar plates and for pigmentation on Granada agar, a specific medium that stabilizes the GBS pigment . We focused our analysis on four mutants displaying a strong decrease in pigmentation and hemolytic activity on Granada and Blood agar plates (see Figure 1A for two of these four phenotypically identical mutants). The four mutants have unique and independent Himar1 insertions in the uncharacterized gene gbs1532 as revealed by direct sequencing on chromosomal DNAs. This 921-bp gene encodes a 306 amino acid protein annotated as a hypothetical protein or putative protease (NCBI ref. seq. NP_735969). Transposon insertions of the four Himar1 mutants were in positions 85, 347, 625, and 632 in the DNA coding sequence, leading to truncated proteins at codons 29, 116, 209, and 211, respectively.
(A) ß-hemolytic and pigmentation phenotypes of NEM316 wild-type strain (WT) and of two independent insertional mutants (abx1::Him1 and abx1::Him2) obtained by random transposon mutagenesis. Strains contained the empty vector pTCV or the complementing vector pTCVΩabx1 where abx1 is transcribed from its own promoter. Pigment production was assayed on Granada agar plates and hemolytic activities on Columbia horse blood (5%) agar plates. The clear halos surrounding bacterial colonies (black dots) on blood plates correspond to lysed erythrocytes. Plates are supplemented with erythromycin (10 µg/ml) for plasmid maintenance. (B) Phenotypes of in-frame abx1 deletion mutant (Δabx1). Strains contained the same vectors as in (A). Overnight cultures in TH were diluted in fresh media and approximately 103 and 102 CFU were spotted on TH, Granada, and Columbia horse blood agar plates plus erythromycin. Pictures were taken after 24–36 h of growth at 37°C. (C) Hemolytic and pigmentation phenotypes associated with abx1 overexpression. WT strain with the empty vector pTCV, the complementing vector pTCVΩabx1, the overexpressing vectors pTCVΩPcyl+_abx1 or pTCVΩPtet_abx1, the negative control vectors pTCVΩPcyl+_gbs1037 or pTCVΩPcyl+_EGFP were spotted onto TH, Granada, and blood agar plates containing erythromycin. An overexpression mutant where the abx1 chromosomal promoter was replaced by the strong Pcyl+ promoter (ΔPabx1::Pcyl+) was similarly spotted on agar plates without antibiotic. TH and Granada plates were photographed after 24–36 h of growth, while blood agar plates were photographed after 16 h of growth at 37°C.
Blast similarity searches, domain analyses, and topology prediction (Figure S1) revealed that Gbs1532 belongs to a conserved superfamily of putative membrane-bound metalloproteases related to eukaryotic CaaX proteases , . The characteristic domain of this superfamily of more than 5,500 proteins is the Abi-domain (Pfam02517). To our knowledge, none of the bacterial Abi-domain proteins are functionally characterized to date . We therefore named the translated gbs1532 gene product Abx1, for Abi-domain protein related to CaaX protease, and renamed the corresponding gene accordingly (i.e., abx1). This abx1 gene belongs to the core genome of all sequenced GBS strains ,  and 6 other genes coding for Abi-domain proteins similar to Abx1 are present in their genomes (Figure S1).
To confirm that abx1 is required for pigmentation and hemolysis, we constructed in-frame deletion mutant (Δabx1) in the NEM316 WT strain. The Δabx1 mutant displayed the same phenotypes as the Himar1 mutants (Figure 1B). Furthermore, pigment production and hemolysis were restored upon ectopic expression of abx1 transcribed from its own promoter (Figure 1A and 1B: pTCVΩabx1 complementing vector) confirming that abx1 was responsible for the observed phenotypes.
Overexpression of Abx1 increases hemolysis and pigment production
Wild-type or Δabx1 mutant strains expressing Abx1 from the complementing plasmid pTCVΩabx1 were more pigmented and more hemolytic than the control strains (Figure 1B). This suggests that the expression level of Abx1 might be limiting for pigment production and hemolysis. To test this hypothesis, we cloned abx1 downstream of two constitutive promoters with different strength (Ptet and Pcyl+) to generate the overexpressing vectors pTCVΩPtet_abx1, and pTCVΩPcyl+_abx1, respectively. Furthermore, we engineered the WT strain to replace the chromosomal Pabx1 promoter by the Pcyl+ promoter (ΔPabx1::Pcyl+). All recombinant strains carrying the different overexpression forms of abx1 displayed hyper-hemolysis on blood agar and hyper-pigmentation on Granada agar (Figure 1C). Determination of the hemolytic titer of the different mutants and quantification of the corresponding abx1 transcription levels further confirmed the link between hemolysis and abx1 expression (Table 1). Remarkably, the pigmentation level also correlated with the strength of the abx1 upstream promoter (Ptet>Pcyl+>Pabx1) on TH agar, a medium where pigmentation is usually not observed (Figure 1C). Overexpression of Gbs1037, the closest Abx1 homolog in GBS (40% identity over 222 residues, E-value = e-11; Figure S1) and of the unrelated EFGP (Enhanced Green Fluorescence Protein) did not modify pigmentation and hemolysis of NEM316, underlining the specific activity of Abx1 (Figure 1C). The activity of Abx1 is a conserved feature within the GBS species since abx1 deletion or overexpression in several clinical strains (serotypes Ia, 515; Ib, H36B; II, 18RS21; III, BM110; and V, 2603V/R; ) has a strong effect on their hemolytic activities and pigmentation (Figure S2).
Induction of hemolysis by Abx1 is not achieved by prenylation- or protease-activity
In eukaryotes, Abi-domain-containing proteins (Pfam02517) are CaaX prenyl proteases involved in protein prenylation , , , . These transmembrane proteases remove the carboxy-terminal aaX tripeptide of a protein after the addition of an isoprenyl group on the cysteyl residue of the CaaX motif. One of the main features of the Abi-domain is the presence of four predicted core transmembrane helical segments (labeled TMH1–4) containing conserved active-site residues (Figure S1D). The overall topology and the critical residues for protease activity are conserved in Abx1 (Figure 2A and S1). To date, prenylation was never described in prokaryotic cells but, intriguingly, the CylE hemolysin contains a cysteyl residue at the fourth position from its carboxylic end (i.e., the critical residue of the CaaX motif ).
(A) Abx1 is a member of a large family of proteins related to eukaryotic CaaX proteases , . The three most conserved motifs of the Abi-domain (Pfam02517) are depicted for Abx1 and Gbs1037 from S. agalactiae, Spr1119 from S. pneumoniae, SagE from S. pyogenes and for two eukaryotic CaaX proteases, the budding yeast ScRCE1 and the human HsBRCA2. Conserved amino acids essential for CaaX protease activity are in red with the putative catalytic glutamic acid (E) residue shown by a plus (+). Figure S1 provides additional information on the Abi-domain family. (B) The CylE hemolysin is essential for Abx1 function on hemolysis and pigmentation. GBS hemolysis and pigmentation were observed on TH, Granada (Gr) and Blood agar (Bl). Strains are: (a) in-frame cylE deletion mutant (ΔcylE) with the empty vector pTCV or the abx1 overexpression vector pTCVΩPcyl+_abx1; and (b) WT, abx1 deletion mutant (Δabx1) and a mutant with a cysteyl to alanyl substitution in the CaaX motif of CylE (CylE C664A), i.e., the putative prenylation site of CylE. (C) Conserved amino acids critical for CaaX protease activity are not required for Abx1 activity. The non-hemolytic and apigmented Δabx1 mutant were complemented with pTCVΩabx1 vectors encoding a wild-type allele (Abx1) or several alanine substitution alleles in the predicted critical glutamic acid (E) or histidine (H) residues.
We tested whether CylE could be post-translationally modified by a prenylation-like mechanism involving Abx1. Overexpression of Abx1 using pTCVΩPcyl+_abx1 in the non-hemolytic and non-pigmented ΔcylE mutant did not increase hemolysis or pigmentation (Figure 2B), confirming that Abx1 function is strictly dependent on the presence of a functional CylE. We then substituted the cysteyl residue of CylE located at the fourth position from its carboxy-terminal end by an alanyl (CylE C664A). This mutation did not alter pigmentation and hemolysis significantly (Figure 2B), which suggests that CylE is not prenylated on this cysteyl, and consequently that Abx1 might not be a CaaX protease. To further test whether Abx1 has protease activity, we replaced the conserved residues (glutamyl at positions 164 and 165 and histidyl at positions 197 and 235) described as critical for this enzymatic activity by alanyl residues (Figure 2A; ). All alleles obtained for Abx1 (E164A, E165A, EE164/165AA, H197A, H235A) restored hemolysis and pigment production when expressed in a Δabx1 mutant strain (Figure 2C). Hence, the putative protease activity of Abx1 is not necessary for the CylE dependent phenotypes.
Abx1 and CovR, the major regulator of virulence gene expression, are functionally linked
Abx1 activity is dependent of CylE but appears to be independent of a CylE post-translational modification. To decipher the relationship between Abx1 and cyl-dependent hemolysis and pigment production, we took advantage of the previously described ΔCBSCyl mutant where the CovR binding sites (CBS) in the promoter of the cyl operon were deleted . Since CovR acts as a transcriptional repressor of the cyl operon, deletion of CBSCyl leads to strong constitutive expression of the cyl operon. Consequently, the ΔCBSCyl mutant is hyperhemolytic and hyperpigmented in all tested conditions (Figure 3A). Whereas abx1 deletion in a WT background abolishes pigmentation and hemolysis, deletion of abx1 in a ΔCBSCyl background (Δabx1ΔCBSCyl) did not affect the hyper-hemolytic and hyper-pigmented phenotypes of the parental ΔCBSCyl strain (Figure 3A). This result suggests that Abx1 acts upstream of CovR-mediated transcriptional inhibition of the cyl operon.
(A) Control of hemolysin and pigment production by Abx1 is dependent on the two-component system CovSR. Phenotypic comparison of WT and abx1 mutant without or with mutations in CovR (ΔcovR), CovSR (ΔcovSR), and in the CovR binding sites of the cyl operon (ΔCBScyl), as assayed on TH, Granada (Gr) and Blood agar (Bl) plates. (B) CovR-regulated genes are similarly expressed in abx1-overexpressing and covR-deletion mutants. Immunoblots of the surface-exposed adhesin BibA (Gbs2018) and secreted CAMP factor (Gbs2000) in cell wall extracts and concentrated supernatants, respectively. The Bsp protein was used as a loading control. Specificities of BibA and CAMP factor antibodies were confirmed with extracts from deletion mutants of the corresponding genes (negative controls).
To confirm this epistatic interaction, we inactivated covR (gbs1672) and the complete covSR two-component system (gbs1671–1672) in the WT and Δabx1 backgrounds. As expected, inactivation of covR or covSR in the WT strain led to hyper-pigmentation and hyper-hemolysis (Figure 3A). Similarly, inactivation of CovR or CovSR in the Δabx1 background restored pigmentation and hemolysis to levels similar to those observed in the ΔcovR and ΔcovSR mutants (Figure 3A). This suggests that Abx1 is an inhibitor of CovR activity. Consistently, Western blot analysis of the adhesin BibA and CAMP factor whose expression are negatively and positively regulated by CovR , , respectively, revealed similar expression patterns in the ΔcovR and abx1 overexpression mutants: inactivation of CovR or overexpression of abx1 dramatically increased BibA production and abolished secretion of the CAMP factor (Figure 3B). These results demonstrate that Abx1 activity is not restricted to pigmentation and hemolysin production, and suggest an antagonist function on CovR activity.
Abx1 inhibits CovSR signaling by antagonizing CovS
To decipher the relationship between Abx1 and the CovSR two-component system, we compared the transcriptomes of ΔcovS, ΔcovR and ΔcovSR mutants with the Δabx1 deletion mutant and two abx1 overexpression mutants (v_Oe_abx1 = vector based abx1 overexpression with the pTCVΩPcyl+_abx1 plasmid; and K_Oe_abx1 = chromosomal abx1 overexpressing strain ΔPabx1::Pcyl+). Pairwise comparisons of Log2 expression ratios (Table S1, N = 1,905 genes) revealed highly similar expression changes between the strain carrying the chromosomal overexpression of abx1 (K_Oe_abx1) and the ΔcovS and ΔcovR deletion mutants (Figure 4A: Pearson correlation = 0.704 and 0.727, respectively). Strikingly, the transcriptome profiles of the abx1 overexpression and ΔcovS deletion mutants form a distinct cluster as revealed by hierarchical clustering analysis (Figure 4B and S3A).
Transcriptome analysis of deletion (Δabx1, ΔcovS, ΔcovR, and ΔcovRS) and overexpressing (Oe_abx1) mutants. The latter mutants were obtained either by chromosomal replacement of the abx1 WT promoter by the strong constitutive promoter Pcyl+ (K_Oe_abx1) or by using the overexpression vector pTCVΩPcyl+_abx1 (v_Oe_abx1). The WT strain with the empty vector (NEM_ery) was included to take into account the potential effect of erythromycin (10 µg/ml) used to maintain the abx1 overexpression vector. (A) Pairwise comparisons of Log2 expression ratios for 1,905 genes between abx1 overexpressing mutant (K_Oe_abx1) and ΔcovR (blue dots) or ΔcovS (red dots) deletion mutants. Pearson's correlations (r2) were calculated to estimate similarities in gene expression change. (B) Heatmap of the genes (N = 147) with an absolute log2 ratio >2 in at least one strain. Gene expression changes were color-coded (blue = down; yellow = up). Hierarchical clustering (uncentered; average linkage) was done for genes (upper tree) and strains (right tree). Genes of the CovSR regulon conserved in 3 sequenced GBS strains (NEM316, A909, and 2603V/R) are highlighted below the heatmap (blue line = down-conserved; yellow line = up-conserved). (C) Highlight of the gene cluster in (B) containing the conserved negatively regulated genes of the CovSR regulon (in dark red letters). The gene tree is shown on the left of the heatmap and the corresponding gene identifiers and protein annotations are indicated on the right. Genes marked with a star were selected for confirmation of gene expression by qRT-PCR. Enlarge version of (B) and (C) heatmaps at a lower threshold ( = absolute log2 ratio >1 in at least one strain) are presented in supplementary figure S3. (D) qRT-PCR expression analysis of selected genes in abx and cov mutants. The relative expression level of abx1, of 5 negatively-regulated CovR genes (cylE, cylJ, bibA, gbs0791 and gbs1037), and of one positively-regulated CovR gene (cfb coding the CAMP factor) were measured in Δabx1, ΔcovS, and ΔcovR deletion mutants, in the WT and Δabx1 mutant containing the abx1 complementing vector (+ abx1; plasmid pTCVΩabx1), and in two abx1 overexpressing mutants (K_Oe_abx1 = chromosomal abx1 overexpression; v_Oe_abx1 = vector-based abx1 overexpression with the pTCVΩPcyl+_abx1 plasmid). Results are the relative expression level between mutant and WT strains expressed as the means value (+/− SEM) from three independent cultures in triplicates.
Detailed analyses revealed a cluster of genes containing the direct and conserved targets that are negatively regulated by CovR (Figure 4C and S3B: N = 29 and 40 genes with expression change log2>2 and >1 in at least one strain, respectively). Expression of nearly all of these genes, including the cyl operon (gbs0644–gbs0655) and the bibA gene (gbs2018), were dependent on the expression level of abx1. Moderate overexpression of abx1 in the chromosome (ΔPabx1::Pcyl+; 3.7 fold change by microarrays) induced a transcriptome response highly similar to that of the ΔcovS mutant (Figure 4C). Greater overexpression of abx1 (pTCVΩPcyl+_abx1; 9.1 fold change by microarrays) further increased expression of the direct-CovR regulated gene set to a level close to that observed in a ΔcovR mutant (Figure 4C). In contrast, downregulation below the significant threshold is observed in the Δabx1 mutant (Figure 4C). qRT-PCR analysis on a set of 6 genes regulated by the CovSR system confirmed gene expression changes identified by microarray analysis (Figure 4D). Moreover, bibA and CAMP factor transcription levels in the ΔcovR deletion and abx1 overexpression mutants are in accordance with the corresponding protein expression levels as observed by Western analysis (Figure 3B). Taken together, the similarities between abx1 overexpression mutants and cov deletion mutants suggest that Abx1 exerts a specific effect on the CovSR two-component system, most likely by acting as a CovS antagonist.
Abx1 interacts directly with the histidine kinase CovS
Our results suggest an inhibitory function of Abx1 on CovS activity or that Abx1 protects or sequesters CovR, impeding its phosphorylation by CovS either directly or indirectly. To discriminate between these possibilities, we used a bacterial two-hybrid system  to test the physical interactions of Abx1 with different putative partners. This showed that Abx1 is able to form homodimers and that it interacts indeed with CovS but not with CovR (Figure 5A and 5B). The Abx1-CovS interaction is specific, as Abx1 did not interact with two other GBS HKs that are similar to CovS (Figure 5A: Gbs2082 and Gbs0430). To define the regions involved in the Abx1-CovS interaction, we tested the interaction of Abx1 with different domains of CovS (Figure 5C). These experiments showed that Abx1 interacts specifically with the amino-terminal part of CovS containing its extracytoplasmic and transmembrane domains (Figure 5D). Deletion of the extracytoplasmic loop of CovS did not impede interaction with Abx1, suggesting that the loop is not directly required for Abx1-CovS interaction (Figure 5D: CovS form VI). No interaction was detected either with the carboxy-terminal part of CovS containing the catalytic domains or with CovS truncated forms containing only one transmembrane domain (Figure 5D). These results suggest that Abx1 interacts with the two CovS transmembrane domains involved in signal processing.
The physical interactions between proteins were assayed using a two-hybrid system in E. coli. Fusion proteins were constructed with the T18 or the T25 adenylate cyclase fragments. The activity of the ß-galactosidase cAMP-dependent reporter gene, expressed in arbitrary units (AU), was determined as described in Materials and Methods. Means and standard deviations are calculated from at least three independent cultures. (A) Interactions between Abx1 and the transcriptional regulator CovR, the histidine kinase CovS, and two others GBS histidine kinase (Gbs2082 and Gbs0430). The T18 and the T25 tags are located at the C-terminal end of tested proteins. Interactions of Abx1-T18 (dark blue) were measured against the indicated T25 tag protein. Reciprocally, interactions of Abx1-T25 (light blue) were measured against the indicated T18 tag protein. (B) Homo-dimerizations were measured by the interaction between the T18 and T25 fusions of the same proteins and are show with light grey bars. The specific interaction between the HK CovS and its RR CovR is shown with dark grey bars. (C) Schematic representation of the CovS histidine kinase domains and of the truncated alleles used in (D). The amino-terminal part of CovS (residues 1–211) is made of two transmembrane (TM) domains flanking an extracellular loop. The cytoplasmic carboxy-terminal part of CovS (residues 211–501) contains the typical HAMP, HisKA and HATP regulatory and catalytic domains. Truncated alleles (I to VI) were tag with either T18 or T25 at the C-terminal end (I to III) or at the N-terminal end (IV to VI). A full length CovS allele tagged at the N-terminal end (VII) was added as a control. (D) Interactions between Abx1 and truncated alleles of CovS. The CovS alleles with a C-terminal tag were tested against the corresponding Abx1 tagged at its C-terminal end (blue). Similarly, the CovS alleles with a N-terminal tag were tested against the corresponding Abx1 tagged at its N-terminal end (red).
Abx1 function is dependent on CovS histidine kinase/phosphatase activities
In agreement with the proposed inhibitory function of Abx1 on CovS, deletion of covS in a Δabx1 background (Δabx1ΔcovS) restores pigment production and hemolysis of the non-pigmented and non-hemolytic Δabx1 mutant to levels similar to those of the ΔcovS mutant (Figure 6A). However, as seen previously by transcriptome analysis, the ΔcovS mutant is not identical to the ΔcovR mutant. In particular, the ΔcovR mutant is hyper-pigmented and hyper-hemolytic (see Figure 3A) while the ΔcovS mutant is only slightly affected in pigmentation and hemolysis compared to the WT strain (Figure 6A).
(A) CovS is epistatic to Abx1. Phenotypes of the WT strain, Δabx1, Δabx1ΔcovS, and ΔcovS deletion mutants. (B) CovS kinase/phosphatase activities are essential for CovR regulation. Phenotypes of ΔcovS deletion mutant were compared with those of critical point mutants of CovS (H278A and T282A) or CovR (D53A). The histidine residue number 278 (H278) is the conserved phospho-accepting residue essential for the autophosphorylation of CovS. The threonine number 282 (T282) is a conserved residue necessary for the phosphatase activity of several HKs. The aspartate number 53 (D53) is the conserved residue of CovR phosphorylated by CovS. (C) Abx1 is dependent of CovS activities. Phenotypes of WT, ΔcovS, and CovS H278A or CovS T282A alanine substitution mutants overexpressing abx1 (+Pcyl+_abx1 = plasmid pTCVΩPcyl+_abx1). The hyper-pigmentation and hyper-hemolysis associated to abx1 over-expression in WT strain are suppressed in ΔcovS and CovS H278A mutants. Reversion of CovS H278A and T282A substitution back to the WT CovS sequence (indicated by _WT) restores the abx1 over-expression associated-phenotypes. All assays were performed on TH, Granada (Gr) and blood agar (BI) plates. (D) abx1 overexpression effects are dependent of CovS enzymatic activities. Relative expression levels of selected genes were measured by qRT-PCR in logarithmic growing cells in TH media. Relative expression levels of abx1, of five negatively regulated CovR genes (cylE, cylJ, bibA, gbs0791 and gbs1037), and of one positively regulated CovR gene (cfb coding the CAMP factor) were measured in CovS and CovR alanyl substitution mutant, and in WT and CovS alanyl substitution mutants overexpressing abx1 (+Pcyl+_abx1 = plasmid pTCVΩPcyl+_abx1). Results are means +/− sem from three independent cultures in triplicates.
To explain these observations, we must consider the three common activities of histidine kinases (HK): auto-phosphorylation of the kinase on a histidyl residue, phospho-transfer on an aspartyl residue of the cognate regulator, and phosphatase activity of the HK on its cognate regulator . Deletion of covS abolishes the three activities. In this case, cross-phosphorylation of the regulator by small metabolites or by a non-cognate HK can be observed due to the absence of the phosphatase activity of the cognate HK , . Thus, in the ΔcovS mutant, cross-phosphorylation of CovR might enable its partial activation. To gain experimental evidence, we constructed CovS and CovR alanyl substitution mutants in conserved residues critical for each of the three activities associated with HK . First, we targeted the phospho-acceptor histidyl residue H278 to obtain an auto-phosphorylation deficient allele of CovS. Second, we replaced the aspartyl residue D53 of CovR normally phosphorylated by CovS to abolish phospho-transfer between CovS and CovR , . Third, we replaced the CovS threonyl residue T282 that was proposed to be specifically involved in HK phosphatase activity , . We then used pigmentation and hemolysis production as reporters of CovSR activity (Figure 6B).
The CovR D53A mutant displayed a hyper-hemolytic and hyper-pigmented phenotype like the ΔcovR mutant (Figure 6B), as also observed previously in another GBS strain , supporting the model of CovR as a transcriptional repressor under its D53P phosphorylated form. Analyses of the CovS H278A mutant strain showed phenotypes similar to the ΔcovS mutant (Figure 6B), suggesting that the H278 residue is essential for all three activities, as assumed by a mechanistic model of HK activity , . We identified in CovS the conserved threonyl residues specifically required for the phosphatase reaction of several HK , . Interestingly, the CovS T282A mutant is non-hemolytic and non-pigmented (Figure 6B) indicating that CovR is locked in an active form that fully inhibits the expression of the cyl operon. Overexpression of abx1 in the ΔcovS, CovS H278A or CovS T282A mutants did not modify their phenotypes, indicating that Abx1 activity is dependent on CovS kinase/phosphatase activities (Figure 6C). The fact that overexpression of abx1 in the WT context led to hyper-pigmented and hyper-hemolytic phenotypes, as seen for the ΔcovR and CovR D53A mutants (Figure 6), suggests that Abx1 actively stimulates CovS-dependent dephosphorylation of CovR. Expression level measurements of selected CovR-regulated genes (cylE, cylJ, bibA, gbs0791, gbs1037, and cfb coding the CAMP factor) in CovS and CovR alanyl substitution mutants confirm that the broad effect of abx1 overexpression depends upon a functional CovS histidine kinase (Figure 6D). Overall, our results support a model where Abx1 inhibits the kinase-competent form and/or stabilizes the phosphatase-competent form of CovS.
Abx1-CovS and Stk1 converge to regulate CovR activity
An additional regulator of the CovSR system is the eukaryotic-like serine/threonine kinase Stk1. It has been shown in vitro that Stk1 regulates CovR negatively by direct phosphorylation of the threonyl residue at position 65 (T65) , . The CovR T65 phosphorylation by Stk1 has been proposed to be mutually exclusive of CovR D53 phosphorylation by CovS . Consistently, the Δstk1 (Δgbs0307) mutant is strongly and negatively affected in pigment production and hemolytic activity (Figure 7), in agreement with an inhibitory role of Stk1 on CovR activity.
Phenotypes associated to the inactivation of the serine/threonine kinase Stk1 in a WT background (Δstk1) and in combination with CovS inactivation (Δstk1ΔcovS). All strains were transformed with the empty vector pTCV or derivatives. To complement the Δstk1 mutant, the two-gene operon containing stk1 was cloned with its promoter into the pTCV vector (pTCVΩstp-stk). To compare the effect of Abx1 on CovR-dependent phenotypes, the Abx1 complementing vector (pTCVΩabx1) or the Abx1 over-expression vector (pTCVΩPcyl+_abx1) were introduced into relevant strains. Increased pigmentation and hemolysis due to the presence of the abx1 vectors are indicated with a plus sign (+), absence of effect of the abx1 vectors are indicated by a minus sign (−), and condition-dependent toxicity of abx1 overexpression specifically on Granada medium is indicated by a mark (#). All assays were performed on TH, Granada (Gr) and blood agar (BI) plates.
To learn whether Abx1 and Stk1 function together, we tested if Abx1 and Stk1 interact using the bacterial two-hybrid system. No interaction was observed (data not shown). Furthermore, pigmentation and hemolysis were restored in the non-pigmented and non-hemolytic Δstk1 strain upon increasing abx1 copy number with plasmid pTCVΩabx1 (Figure 7). This indicates that Abx1 is able to bypass CovR activation observed in the absence of Stk1. However, pigmentation and hemolysis levels of the Δstk1 strain carrying the pTCVΩabx1 vector are below those of the WT strain (Figure 7). This result suggests that abx1 overexpression needs a functional Stk1 to fully inhibit CovR activity.
Finally, a condition-dependent toxicity was observed when the overexpressing pTCVΩPcyl+_abx1 vector was introduced into the Δstk1 mutant. Specifically, strong overexpression of abx1 in Δstk1 is toxic on Granada agar (Figure 7). This phenotype is CovS-dependent as toxicity is alleviated in a Δstk1ΔcovS double mutant, which is unresponsive to abx1 overexpression (Figure 7). This result reinforces the link between Abx1 and a functional CovS. In addition, the Δstk1ΔcovS mutant is locked in an intermediate phenotype (Figure 7), revealing a partial inhibition of CovR in the absence of CovS. Thus, CovS appears necessary for complete activation of CovR in the absence of Stk1, most likely by an active CovR D53 phosphorylation. As shown in the model depicted Figure 8, we propose that the regulatory function of Abx1 is absolutely dependent on CovS but also depends on the serine/threonine kinase Stk1 to control CovR activity by a convergent signaling pathway.
(A) Simplified wiring diagram of the regulatory network controlling GBS hemolysin and pigment production. In a WT context, the Abi-domain protein Abx1 maintains the equilibrium between the kinase (green arrow) and the phosphatase activity (red lines) of the HK CovS. Activation of the RR CovR by CovS-dependent phosphorylation increases its inhibitory activity on cyl operon transcription. Inhibition of CovR is dependent on its dephosphorylation by CovS and its phosphorylation by the Stk1 serine/threonine kinase. In this condition, the cyl operon is expressed at a level defining the WT β-hemolytic and pigmentation phenotype. (B) In the absence of Abx1, the HK CovS is locked in its kinase-competent form that activates CovR, thus inhibiting the expression of the cyl operon. Phosphorylation of CovR by CovS precludes the Stk1-dependent CovR phosphorylation, leading to minimal CovR inhibition by Stk1. In this condition, GBS are non-hemolytic and non-pigmented. (C) In the presence of an excess of Abx1, the kinase-competent form of CovS is inhibited and/or the phosphatase-competent form is stabilized. The kinase Stk1 is necessary to fully inactive CovR, thereby allowing a strong expression of the cyl operon. In this condition, GBS are hyper-hemolytic and hyper-pigmented. (D) An integrated model for CovSR signaling with modular schematization of CovS (blue), Abx1 (green), Stk1 (red) and CovR (purple). The CovR activity reflects the equilibrium between the mutually exclusive ATP-dependent phosphorylation of two critical residues: threonyl 65 (T65) by Stk1 and aspartyl 53 (D53) by CovS, as proposed in , . This equilibrium is dependent on Abx1, which is essential for CovS-dependent inactivation of CovR (red arrow). Modulation of CovR affinities for DNA by phosphorylation regulates the expression of target genes, among which those necessary for stress resistance, host adhesion and damages characteristic of invasive bacterial forms. Potential connections on the core of the signaling network are in grey (other Stk1 targets, putative Stp phosphatase activity, putative interaction of CovS-Abx1 with other HKs, putative CovR phosphorylation by small phosphate donor as acetyl-phosphate).
abx1 expression level is critical for virulence
The CovSR two-component system is a master regulator of GBS virulence gene expression. In order to assess the role of Abx1 in GBS virulence, neonatal rat pups were infected by intraperitoneal injection of 5×106 bacteria of either the Δabx1 deletion or abx1 overexpressing mutants. In this model, both mutants exhibited reduced virulence compared to the parental WT strain (Figure 9). It is noteworthy that the abx1 overexpression mutant is totally avirulent (100% survival) and that neonates did not display any sign of illness during the course of two independent experiments (data not shown). These results show that abx1 expression at an appropriate level is required to develop GBS infection.
Mortality curves in neonate rats infected with the WT strain (black circle), the Δabx1 deletion mutant (blue square), and the K_Oe_abx1 overexpressing mutant (red triangle). Two days rat pups were inoculated by intraperitoneal injection of 5×106 bacteria. The results shown are the mean of two independent experiments (2×10 rats per group).
The CovSR two-component system (TCS) is the major regulator of virulence gene expression in GBS. In this study, we identified the Abx1 transmembrane protein as essential and limiting for CovSR activity due to its interaction with the CovS histidine kinase (HK). Regulation of HK activity by an interacting protein is increasingly recognized when studying TCS signaling in a cellular context –. Instead of being a linear signaling pathway, our data show that the CovSR TCS is embedded in a regulatory network involving several regulatory proteins, including the Abi-domain protein Abx1.
Abx1 as a histidine kinase regulator
We first observed that Abx1 is required for hemolysin and pigment production in GBS and that abx1 overexpression in a WT background increases hemolysin and pigment production in a dose-dependent manner. These abx1 effects depend upon a functional CovSR pathway, which directly controls expression of the cyl operon. In agreement with a regulatory activity of Abx1 on the CovSR system, modulation of abx1 expression affected not only hemolysin/pigment production but also production of the adhesin BibA and the CAMP factor, two direct targets of CovR , . Indeed, genome-wide transcriptome profiling suggested a specific and antagonistic activity of Abx1 on the CovSR system.
By combining genetic analysis and protein-protein interactions, we show that Abx1 acts through the HK CovS to control the activity of CovR. The transmembrane protein CovS is the cognate HK of CovR. However, in contrast to the majority of TCS, CovR is mainly a transcriptional inhibitor when phosphorylated on the conserved D53 residue and CovS is assumed to be primarily a phosphatase necessary to relieve repression of virulence genes during infection –, . Our data support this regulatory logic (i.e., the inactivation by CovS of the CovR inhibitor) but show in addition that Abx1 exerts a cellular control on the dual kinase/phosphatase activity of CovS (Figure 8). By using the cyl operon as reporter of CovR activity, we observed constitutive CovR activation with a CovS allele (CovS T282A) mutated in the conserved threonyl residue specifically involved in the phosphatase activity of several other HKs ,  or by deletion of Abx1 (Figure 8B). Conversely, CovR can be inactivated by a D53A substitution, mimicking a non-phosphorylated allele, or by overexpression of Abx1 (Figure 8C). Furthermore, all of these Abx1 effects are dependent on a functional CovS. Taken together, these results suggest that the level of Abx1 is critical to control the equilibrium between the kinase and phosphatase activities of CovS.
It is noteworthy that inactivation of both CovS kinase and phosphatase activities, either with a CovS H278A allele or by deleting covS, led to intermediate phenotypes compared to those obtained by CovR inactivation. This pattern has been observed previously in GBS  and GAS for the CovSR ortholog , , . In this condition, it is likely that CovR is partially activated by cross-phosphorylation, either by small metabolites like acetyl-phosphate or by a non-cognate HK. The specific phosphatase activity of CovS avoids inappropriate activation of CovR by other pathways, a general mechanism to insulate TCS signaling pathways .
Importantly, HK phosphatase activity is not simply the reverse reaction of the kinase activity , . The two activities involve different, but potentially overlapping, catalytic residues  and are mutually exclusive since they depend on different conformations of HK homodimers . Both Abx1 and CovS are transmembrane proteins. We found that Abx1 interacts directly with CovS input domains (i.e., the extracellular and transmembrane domain), and ompacts CovS output domains (i.e., the catalytic cytoplasmic domain). Current models of HK activity regulation are based on different conformational states of HK homodimers . It is therefore most likely that Abx1 interferes with the dynamic process of CovS conformational changes propagated along the whole proteins to control the kinase/phosphatase switch . In the absence of Abx1, CovS appears to be locked into a kinase-competent conformation while overexpression of Abx1 will result in stabilization of the phosphatase-competent conformation (or destabilization of the kinase-competent form). Of note, the CovS extracellular loop is not necessary for the physical interaction with Abx1 and it is therefore likely that the main domains of CovS interacting with Abx1 are the transmembrane domains flanking this loop. These HK transmembrane domains control the conformational rotation of the cytoplasmic subunits of HK homodimer by a rotation or a piston mechanism , . Thus, intra- and inter-molecular interactions interfering with this dynamic process will impact the catalytic activity by blocking HKs in a kinase or a phosphatase form , .
Positive and negative regulation of HK activity by physical interactions with transmembrane regulators has been described in both gram-negative and gram-positive bacteria , . Members of this category are mainly small hydrophobic peptides, like the PhoQ-B1500/SafA , PhoQ-MgrB  and EnvZ-MzrA  pairs in enteric bacteria, or proteins with a single transmembrane domain, like the YycH and YycI proteins regulating the essential HK YyfG in Bacillus subtilis , . In particular, structural modeling suggests that the YyfG HK is inactivated by its interactions with YyfH and YyfI in the membrane environment . In analogy, this suggests a mechanism of CovS inactivation by its interaction with the eight predicted transmembrane spanning domains of Abx1 (Figure S1).
The CovS-Abx1 signaling module
Abx1 controls GBS virulence gene expression by regulating CovS activity, via a mechanism independent of the direct sensing of an environmental signal by CovS. This cellular regulation of TCS by a “third” component raises the question of how this additional regulator is itself regulated , . The HK regulator may set up a feedback loop when located in the same operon as the HK, as for B. subtilis YycHI , , or it may act as a TCS connector when its transcription is controlled directly by another TCS  as for E. coli B1500/SafA, MgrB, and MzrA peptides mentioned above –. We did not find evidence that abx1 transcription is directly controlled by CovR (data not shown) but cannot exclude transcriptional regulation by an as yet unidentified RR.
An alternative hypothesis would be an allosteric regulation and/or a scaffolding function of Abx1 . Regulation of HK activity by signal(s) not directly recognized by the HK but sensed and/or transmitted by its interactor(s) might be a common mechanism, as already described for antimicrobial cationic peptide resistance modules in Firmicutes ,  and for HK-HK complex formation in Pseudomonas aeruginosa . Until now, it is not known how CovS activity is regulated in GBS. In GAS, the CovS ortholog (displaying 50.3% identity with the GBS CovS) is thought to be a direct sensor of magnesium and of the human antimicrobial peptide LL-37 , , . However, only two Abi-domain proteins are present in GAS genomes and neither is an Abx1 ortholog (Figure S1). Although the biological function of the CovS orthologs in virulence is conserved , , GAS and GBS inhabit different ecological niches (nasopharynx and skin for GAS; gut and vagina for GBS), suggesting a species-specific response by CovS. The identification of conditions bypassing the Abx1 effects in GBS will be valuable for characterizing the putative signal(s) directly sensed by CovS.
We found that activity of the Abx1-CovS module depends upon a second signaling pathway mediated by the serine/threonine kinase Stk1 , . The two pathways converge on CovR to regulate virulence gene expression (Figure 8). In vitro, direct phosphorylation of the CovR T65 threonyl residue by Stk1 interferes negatively with phosphorylation of the conserved D53 aspartyl residue and decreases CovR affinity for its target promoters , . By genetic analysis, we found that the Abx1-CovS and the Stk1 signaling pathways are dependent on each other to fulfill complete activation or inhibition of CovR. CovR phosphorylation on T65 and D53 are mutually exclusive , strongly suggesting that CovR has three possible states (Figure 8D: D53P T65 = active; D53 T65 = inactive I dephosphorylated; D53 T65P = inactive II). It should be noted that Stk1 has additional direct targets beyond CovR and that its activity depends upon its associated phosphatase Stp1 –. Interestingly, a Δstk1 mutant is affected in cell cycle progression ( and data not shown), as observed in S. pneumoniae and other bacteria , , and Stk1 might respond to the release of cell wall components into the extracellular environment by growing bacteria . Therefore, it appears that CovR integrates at least two types of signals to coordinate virulence gene expression (via Abx1-CovS) with the control of bacterial division (through Stk1).
This integrated system might be necessary to accurately control GBS lifestyle from commensalism to invasive infection . The attenuated virulence of both deletion and overexpression abx1 mutants supports this dynamic view of the infectious process. The survival of animals challenged intraperitoneally with the overexpressing abx1 mutant is reminiscent of the decreased virulence of ΔcovR mutants previously described –. Several virulence genes controlled by CovR are involved at several steps of the infectious process, ranging from stress resistance to adherence to host cell , , , , , . Among the genes directly regulated by CovR, the hemolysin/cytotoxin CylE has key functions . At sub-lytic concentrations, CylE induces an anti-inflammatory response , while high CylE expression induces a pro-inflammatory response . The inappropriate activation of a strong pro-inflammatory response during initial steps of the infectious process might explain, at least in part, the loss of virulence of mutants overexpressing abx1 or inactivated for covR. In contrario, at a latter stage of infection, these mutants can be more virulent due to the cytotoxic effect of CylE on blood cells, as seen with a ΔcovR mutant injected intravenously in mice . Overall, these results indicate that the Abx1-CovS-CovR-Stk1 regulatory network controls the fine-tuning of virulence gene expression which is critical for GBS disease progression.
The bacterial Abi-domain protein family
The demonstration that Abx1 is a cellular regulator of CovS represents a new function for a bacterial Abi-domain protein. The Abi-domain (Pfam02517) of Abx1 is the characteristic domain of a large transmembrane protein family (>5,000 sequences) with up to 15 members per bacterial species , . This family is mostly uncharacterized to date in prokaryotes but includes the eukaryotic type II CaaX proteases involved in protein prenylation , . However, prenylation appears restricted to eukaryotes and the two examples of prenylated bacterial proteins, SifA of Salmonella typhimurium and AnkB of Legionella pneumophila, are bacterial effectors modified by the eukaryotic enzymes after their injection into the host cell –. Our characterization of targeted mutations (Figure 2) did not sustain our initial hypothesis of a prenylation-like modification of the CylE hemolysin. Moreover, the putative catalytic residues inferred from similarities with CaaX proteases are not necessary for Abx1 activity. Given the high conservation of these residues (Figure S1), we cannot rule out that Abx proteins have protease activity. However, the main function of Abx1 as a regulator of the CovS histidine kinase does not require this putative protease activity.. Strikingly, the absence of a protease activity was already suggested for a Staphylococcus aureus Abi-protein involved in lysostaphin resistance . Interestingly, three of the four S. aureus Abi-domain genes were identified recently in a genome-wide screen for altered targeting of cell-surface proteins . These defects are mainly due to decreased transcription of the corresponding genes in the absence of the Abi-domain proteins , suggesting a conserved mechanism in gene transcriptional regulation.
Finally, it should be mentioned that about one-fifth (>1,000) of bacterial Abi-domain proteins are annotated as “abortive infection protein” due to a historical miss-annotation  leading to confusion between Abi phenotypes  and Abi-domain proteins . It was also suggested that Abi proteins are involved in self-immunity against bacteriocins . However, only a small subset of Abi-proteins (less than 1%) is localized in putative bacteriocin-encoding loci  or in operons encoding small toxins , . In particular, the gene encoding the Abi-domain protein, SagE is located in the operon directing synthesis of the S. pyogenes ß-hemolysin known as streptolysin S or SLS , . However, genetic analysis of this locus did not provide any conclusive clues about the SagE function –. Abi-domain proteins localized in toxin operons remain to be experimentally characterized for their involvement in toxin production and/or regulation.
Studies of TCS signaling in cellular context have identified a great variety of additional TCS partners, targeting either the HK or the RR , . In this study, we demonstrated that the Abi-domain protein Abx1 is a regulator of the HK CovS in GBS. The CovSR system was presumed to be a linear signaling pathway. We show here that the CovSR TCS is the core of a signaling network and propose a new regulatory model, depicted in Figure 8, which includes Abx1-dependent CovS regulation and Stk1-dependent CovR regulation , . Deciphering the additional CovSR input/output associated with these cellular regulators might prove useful for understanding the GBS transition from commensalism to virulence. Strikingly, Abx1 belongs to a very large family of multi-spanning transmembrane proteins that remain mostly uncharacterized to date ,  and are highly conserved at the species level. It is therefore tempting to extend our findings to other members of this family. The characterization of other Abx-like proteins in GBS (Figure S1) and in other bacteria will reveal whether they belong to a functionally conserved family of HK regulators.
Materials and Methods
Animal experiments were performed at the Institut Pasteur (Paris, France) animal husbandries in accordance with the policies of the European Union guidelines for the handling of laboratory animals (http://ec.europa.eu/environment/chemicals/lab_animals/home_en.htm) and were approved by the Institut Pasteur animal care and use committee (N°04.118).
Bacterial strains and growth conditions
The reference WT GBS strain used in this study is NEM316, a ST-23 and serotype III clinical isolate responsible for a fatal case of septicaemia, whose sequenced genome  is accessible (NCBI RefSeq NC_004368.1). The relevant characteristics of the bacterial strains and plasmids used in this study are summarized in Table S2. GBS was cultured in Todd Hewitt (TH) broth (Difco Laboratories) at 37°C without agitation. Escherichia coli DH5α (Invitrogen) and XL1 Blue (Stratagene) were grown in Luriani Broth (LB) medium. When specified, antibiotics were used at the following concentrations: for E. coli: ticarcillin, 100 µg/ml; erythromycin, 150 µg/ml; kanamycin, 25 µg/ml; for GBS: erythromycin, 10 µg/ml; kanamycin, 1,000 µg/ml.
Characterization of pigmentation and hemolytic activity
Pigmentation and ß-hemolytic activity were detected on Granada agar and Columbia agar supplemented with 5% horse blood, respectively (BioMérieux, France). Overnight GBS cultures in TH were washed, adjusted to 108 CFU/ml, serially diluted (dilution factor 10) in microplates and spotted on appropriate agar plates. Horse blood plates were incubated at 37°C for 16–24 h, followed by an additional 16–24 h incubation at room temperature or 4°C depending on the hyper- or hypo-hemolytic mutants being tested. To highlight the halo of lysis due to the ß-hemolysin, photographic acquisitions of blood agar plates were taken with light from below. Whole images were further converted to gray scale and processed (Photoshop CS4, Adobe, US) to adjust contrast and brightness. Granada plates were incubated 20–36 h at 37°C in anaerobic condition (AnaeroGen, Oxoid, UK). Photographic acquisitions of TH and Granada plates were done with side lighting from above and whole images were processed for contrast and brightness.
GBS hemolytic titers were determined by a semi-quantitative method as described previously, with slight modifications . Briefly, culture adjusted to 2.108 CFU/ml in Phosphate Buffer Saline (PBS) solution supplemented with 0.2% glucose were serially diluted (dilution factor = 2) in microplates. One volume (100 µl) of 1% defibrinated horse blood (Oxoid, UK) in 0.2% glucose PBS was added in each well and plates were incubated 1 h at 37°C. After gentle centrifugation (5 min. at 1000 rpm) to pellet unlysed cells, the amount of hemoglobin in 100 µl of supernatants was quantified by optical absorbance at 420 nm. Hemolytic activity of each strain was defined as the minimum dilution that lysed at least 10% of red blood cells. Hemolytic titers were defined by the ratio between hemolytic activities of each strain against the hemolytic activity of the WT strain (titer X = activity X/activity WT). Negative and positive controls were PBS ( = 0% lysed cells) and 0.1% SDS ( = 100% lysed cells) instead of bacterial cells, respectively. All assays were performed in triplicates with independent cultures.
DNA manipulation and mutant construction
Purification of GBS genomic DNA and E. coli plasmids DNA was done on columns following manufacturer instructions (DNeasy Blood and Tissue kit and Quiaprep Spin Minipreps kit, respectively, Qiagen). Oligonucleotides (MWG and Sigma) used in this study are listed in Table S3. Analytical PCR used standard Taq polymerase (Invitrogen, Life technologies) and preparative PCR for cloning and sequencing was carried out with a high fidelity polymerase (Phusion, Finnzymes). Sanger sequencing was done at the Institut Pasteur sequencing core facility (Paris, France) using the ABI PRISM 3.1 dye terminator cycle sequencing kit (Applied Biosystems) or outsourced (Beckman Coulter Genomic, UK).
Plasmids for overexpression and complementation (pTCV backbone, Table S2), deletion (pG+host5 backbone, Table S2) and for bacterial double hybrid (pUT18 and pKNT25 backbones, Table S2) were constructed by standard cloning procedures and all inserts were fully sequenced. Primer pairs, DNA matrix and restriction enzymes used for each vector are detailed in Table S4. For several constructs, we used a splicing by overlap-extension method  to generate in-frame deletion cassettes, site-directed mutagenesis inserts or translational fusions (Table S4). For instance, deletion cassettes for chromosomal in-frame deletions were generated by combining two 500 bp PCR products, corresponding to genomic sequences flanking the region to be deleted, that were designed to have 25–50 bp of homology with each other at one end . The 1 kb amplification products obtained with external primers were subsequently cloned into the thermosensitive shuttle plasmid pG+host5 backbone and sequenced (Table S4).
Plasmids were introduced in GBS by electroporation. For chromosomal gene inactivation/modification, allelic exchanges were selected as described , . Deletions and site-directed substitutions were confirmed by sequencing PCR products obtained with primers designed outside the genomic region used for the construction of the corresponding cassette (Table S3 and S4).
Detection of cell wall and secreted proteins
Secreted proteins were purified from 40 ml TH broth cultures collected at mid-exponential phase (DO600 nm = 0.5). Supernatants were filter sterilized and concentrated 50-fold using Vivaspin 20 columns (Sartorius). Cell-wall proteins were prepared from overnight cultures at 37°C in TH by mutanolysin (Sigma-Aldrich) digestion in osmo-protective buffer as described , . Following SDS-PAGE electrophoresis, proteins were transferred onto a nitrocellulose membrane (GE Healthcare) and detected using rabbit specific polyclonal antibodies  and horseradish peroxidase (HRP)-coupled anti-rabbit secondary antibodies. Signals were detected by chemiluminescence (ECL, GE Healthcare).
RNA isolation and analysis
Total RNAs were extracted from exponentially growing cells (OD600 = 0.4–0.5) in TH at 37°C with a phenol/Trizol-based purification method as previously described . Reverse transcription was done with Superscript indirect cDNA kit (Invitrogen, Life technologies) and qPCR was carried out with SYBR Green PCR kits (Applied Biosystems, Life technologies). Relative quantification of specific gene expression was calculated with the 2−ΔΔCt method, with gyrA as the housekeeping reference, and normalized against the NEM316 wild-type. Each assay was performed at least in triplicate on three independent cultures.
For microarray analysis, identical culture conditions and RNA purification procedures were used. The three independent cDNA preparations of each strain were labeled with Cy5 or Cy3 (Amersham Biosciences) for dye swap hybridizations against NEM316 wild-type strain RNA prepared in parallel. The 15K custom microarray (Agilent Technologies) contains 8,691 60-mer oligonucleotides specific for the 2134 predicted genes and long intergenic region of the NEM316 strain . Arrays were scanned in an Axon 4000B dual laser scanner and treated as described . Raw data have been submitted to the ArrayExpress database under the Accession number E-MEXP-3703 (http://www.ebi.ac.uk/arrayexpress/). Hierarchical clustering were done with Cluster 3.0 and visualized with Java TreeView.
Bacterial two-hybrid (BATCH) system
For BACTH assays , chimeric GBS proteins fused to either T18 or T25 fragments of adenylate cyclase (CyaA) were constructed by conventional cloning in pUT18 and pKNT25 vectors for T18/25 C-terminal tagging or in pUT18C and pKT25 for T18/25 N-terminal tagging (Table S2 and S4). Physical interactions were assayed in E. coli DHT1 cells (Table S2) co-transformed with recombinant pKNT25/pKT25 and pUT18/pUT18C vectors. Interaction efficiencies between hybrid proteins were quantified by measuring ß-galactosidase activity in 96-well plates assays. Bacteria were grown overnight at 30°C in 1 ml LB broth in the presence of 0.5 mM IPTG and appropriate antibiotics in 2.2 ml 96-deepwell plates (Thermo Scientific). To permeabilize cells, 100 µl of bacterial cultures in 96 deepwell polypropylene plates were treated with 500 µl of buffer containing ß-mercaptoethanol (50 mM), SDS (0.2% v/v) and chloroform (3% v/v) and vortex two times 1 min (Mixmate, Eppendorf). For the enzymatic reaction, 40 µl of ONPG (4 mg/ml) was added to 120 µl of the permeabilized cells solution in a new microplate. Reaction kinetics at 28°C were followed by recording the OD420 every 4 min for 60–90 min in a microplate reader (Synergy, BioTek). Slopes (min−1) with a correlation coefficient r2≥0.98 were used to calculated enzymatic activities relative to internal negative (pUT18 and pKNT25 empty vectors; activity = O arbitrary units) and positive controls (pUT18-zip and pKNT25-zip vectors, ; activity = 1,000 arbitrary units) incorporated in each microplate. Slopes420 was divided by the OD600 of each culture to normalize for initial cell density. At least four independent cultures were done for each plasmid combination. All recombinant pKNT25/pKT25 and pUT18/pUT18C vectors were tested against the corresponding empty vector and gave background level of ß-galactosidase activity (not depicted in Figure 5).
In vivo virulence assay
Two-days old neonatal Sprague-Dawley rat pups (Janvier, Le Genest Saint Isle, France) were used for mortality curve experiments. Randomized groups of 10 neonatal rat pups were infected by intraperitoneal (i.p.) injection with 5×106 bacteria in 100 µl PBS. Survivals were monitored for five days after injection and two independent experiments were carried out.
Abx1 is a multi-spanning transmembrane protein belonging to a large family related to CaaX prenyl proteases. (A) Prediction of transmembrane domains in the Abx1 sequence by the TMHMM v.2.0 software (Center for Biological Sequence Analysis, Technical University of Denmark). The eight predicted transmembrane domains with a probability greater than 0.5 are illustrated above with red boxes. The four transmembrane domains containing the conserved Abi-domain signature are highlighted with a yellow box. (B) Identification of Abi-domain proteins in the genome of S. agalactiae, S. pneumoniae, and S. pyogenes. Proteins containing the Abi-domain (Pfam domain PF02517, Wellcome Trust Sanger Institute, UK) were retrieved from a representative genome of each species. Protein length, Abi-domain start and end residues used for the alignment, and Abi-domain score and probabilities are given for each protein of this family. For comparaison, two eukaryotic CaaX proteases from S. cerevisiae (ScRCE1) and H. sapiens (HsBRCA2) are included. (C) Cladogram of selected streptococcal Abi-domain proteins. Multiple alignments of full protein sequences and unrooted tree were done with ClustalX 2.1 (University College Dublin, Ireland). Tree branches with a bootstrap value above >0.9 (1,000 repetitions) are indicated with a red diamond. The dark-red diamond represents a bootstrap value above >0.9 when analysing only the Abi-domain of each proteins as represent in (D). S. agalactiae, S. pneumoniae, and S. pyogenes proteins are in blue, red and green letters, respectively. (D) Multiple sequence alignments of streptococcal Abi-domain adapted with the representation of Pei J., Grishin N.V. and co-workers , . The conserved topology included the four predicted transmembrane domains (TMH1 to TMH4) that are mainly made of uncharged residues (yellow background) and each containing a typical motif (motif 1 to motif 4). Highly conserved residues of motifs 1 (EExxxR), 2 (FxxxH) and 4 (HxxxN) are shown in white letters on a black background, while substitutions at these sites are on gray background. Putative cation-binding residues (1 glutamyl, E; 2 histidyl, H) and the predicted catalytic site (1 glutamyl) are marked below the two eukaryotic CaaX prenyl proteases with an asterisk and a plus sign, respectively. Seryl (S) and threonyl (T) residues in THM2 are highlighted with a green background. The more variable motif 3 (sxxxs, where s = small residues) is highlighted with a violet background. This variable motif is useful to classify Abi-domain proteins when related protein super-families (not depicted here) are considered .
Conservation of the Abx1 function at the species level. Deletion (A) and overexpression (B) of abx1 were carried out in the WT strains BM110 (serotype III, ST17), 2603 V/R (serotype V, ST110), 515 (Serotype Ia, ST23), H36B (Serotype Ib, ST6) and 18RS21 (Serotype II, ST19). The abx1 gene is conserved among the core genome of WT GBS isolates. It is 100% identical in the NEM316, 2603 V/R, H36B, and 18RS21 genomes; and differs by 1 SNP in the 515 genome (resulting in one amino acid change T107I); and by 5 SNPs in the BM110 genome (all being silent mutation at the protein level). Deletion mutant (Δabx1) and return to the abx1 WT allele (WTback) were selected after chromosomal integration of the abx1 deletion vector in each strain and the subsequent chromosomal excision of this vector. For abx1 overexpression, each strain was transformed with the pTCV empty vector and the pTCVΩPcyl+_abx1 overexpressing vector (abbreviated Pcyl+_abx1). Serial dilutions (10 fold factor) of cultures were spotted on TH, Granada and Columbia supplemented with 5% horse blood agar plates. Erythromycine (10 µg/ml) was added when necessary for plasmids maintenance. Plates were photographed after 16–36 h of growth.
Transcriptomes profiling of abx1 and covS/R mutants. (A) Heatmap of the genes (N = 688) with an absolute log2 ratio >1 in at leat one strain. Deletion (Δ) for abx1, covS, covR and the double covRS mutants were compared to the abx1 over-expression (Oe) mutants obtained by chromosomal (K_) substitution of the endogenous promoter or with the over-expression vector (V_; plasmid pTCVΩPcyl+_abx1). The WT strain with the empty vector (NEM_ery) was added to take into account the effect of the selection pressure necessary for plasmid stability (erythromycin 10 µg/ml). Hierarchical clustering (uncentered; average linkage) was applied for genes (upper tree) and for arrays/strains (right tree). Gene expression changes were color-coded (blue = down; yellow = up). Main genes clusters are highlighted with red stars on gene tree and red boxes below the heatmaps. A short description of the main characteristic of each cluster (number 1 to 8) is given. (B) Highlight of the gene cluster 1 in (A) containing the genes negatively regulated by CovR conserved in 4 different GBS serotypes, as defined by independent groups , . Gene tree is shown on the left of the heatmap and the corresponding systematic names and short annotations are on the right. The conserved CovR-regulated genes are in dark red letters.
Transcriptome analyses of abx1 and cov mutants. (A) Normalized expression ratio for 1905 genes. (B) Number of genes differentially regulated. (C) Definition of the CovR regulon in NEM316 and A909 WT starins. (D) List of 229 genes excluded from the analysis.
We would like to thank Carmen Buchrieser, Cécile Wandersman and Alexandra Gruss for their critical reading of the manuscript; Daniel Ladant and Gouzel Karimova for plasmids and advice on double hybrid system; Cosmin Saveanu and Thomas Rolland for their expertise and assistance with bio-informatics; and Marcia Dinis for assistance during in vivo experiments.
Conceived and designed the experiments: AF PTC. Performed the experiments: AF AT VDC SB ES. Analyzed the data: AF SD DTG PG CP PTC. Wrote the paper: AF PTC.
- 1. Blaser MJ, Falkow S (2009) What are the consequences of the disappearing human microbiota? Nat Rev Microbiol 7: 887–894.
- 2. Henriques-Normark B, Normark S (2010) Commensal pathogens, with a focus on Streptococcus pneumoniae, and interactions with the human host. Exp Cell Res 316: 1408–1414.
- 3. Verani JR, McGee L, Schrag SJ (2010) Prevention of perinatal group B streptococcal disease–revised guidelines from CDC, 2010. MMWR Recomm Rep 59: 1–36.
- 4. Thigpen MC, Whitney CG, Messonnier NE, Zell ER, Lynfield R, et al. (2011) Bacterial meningitis in the United States, 1998–2007. N Engl J Med 364: 2016–2025.
- 5. Phares CR, Lynfield R, Farley MM, Mohle-Boetani J, Harrison LH, et al. (2008) Epidemiology of invasive group B streptococcal disease in the United States, 1999–2005. JAMA 299: 2056–2065.
- 6. Edmond KM, Kortsalioudaki C, Scott S, Schrag SJ, Zaidi AK, et al. (2012) Group B streptococcal disease in infants aged younger than 3 months: systematic review and meta-analysis. Lancet 379: 547–556.
- 7. Mitchell TJ (2003) The pathogenesis of Streptococcal infections: from tooth decay to meningitis. Nat Rev Microbiol 1: 219–230.
- 8. Nizet V (2002) Streptococcal beta-hemolysins: genetics and role in disease pathogenesis. Trends Microbiol 10: 575–580.
- 9. Lindahl G, Stalhammar-Carlemalm M, Areschoug T (2005) Surface proteins of Streptococcus agalactiae and related proteins in other bacterial pathogens. Clin Microbiol Rev 18: 102–127.
- 10. Nobbs AH, Lamont RJ, Jenkinson HF (2009) Streptococcus adherence and colonization. Microbiol Mol Biol Rev 73: 407–450.
- 11. Lamy MC, Zouine M, Fert J, Vergassola M, Couve E, et al. (2004) CovS/CovR of group B streptococcus: a two-component global regulatory system involved in virulence. Mol Microbiol 54: 1250–1268.
- 12. Jiang SM, Cieslewicz MJ, Kasper DL, Wessels MR (2005) Regulation of virulence by a two-component system in group B Streptococcus. J Bacteriol 187: 1105–1113.
- 13. Jiang SM, Ishmael N, Hotopp JD, Puliti M, Tissi L, et al. (2008) Variation in the Group B Streptococcus CsrRS regulon and effects on pathogenicity. J Bacteriol 190: 1956–1965.
- 14. Gao R, Stock AM (2009) Biological insights from structures of two-component proteins. Annu Rev Microbiol 63: 133–154.
- 15. Lembo A, Gurney MA, Burnside K, Banerjee A, de los Reyes M, et al. (2010) Regulation of CovR expression in Group B Streptococcus impacts blood-brain barrier penetration. Mol Microbiol 77: 431–443.
- 16. Santi I, Grifantini R, Jiang SM, Brettoni C, Grandi G, et al. (2009) CsrRS regulates group B Streptococcus virulence gene expression in response to environmental pH: a new perspective on vaccine development. J Bacteriol 191: 5387–5397.
- 17. Cumley NJ, Smith LM, Anthony M, May RC (2012) The CovS/CovR acid response regulator is required for intracellular survival of group B Streptococcus in macrophages. Infect Immun 80: 1650–1661.
- 18. Park SE, Jiang S, Wessels MR (2012) CsrRS and Environmental pH Regulate Group B Streptococcus Adherence to Human Epithelial Cells and Extracellular Matrix. Infect Immun 80: 3975–3984.
- 19. Graham MR, Smoot LM, Migliaccio CA, Virtaneva K, Sturdevant DE, et al. (2002) Virulence control in Group A Streptococcus by a two-component gene regulatory system: global expression profiling and in vivo infection modeling. Proc Natl Acad Sci U S A 99: 13855–13860.
- 20. Sumby P, Whitney AR, Graviss EA, DeLeo FR, Musser JM (2006) Genome-wide analysis of Group A Streptococci reveals a mutation that modulates global phenotype and disease specificity. PLoS Pathog 2: e5.
- 21. Kansal RG, Datta V, Aziz RK, Abdeltawab NF, Rowe S, et al. (2010) Dissection of the molecular basis for hypervirulence of an in vivo-selected phenotype of the widely disseminated M1T1 strain of Group A Streptococcus bacteria. J Infect Dis 201: 855–865.
- 22. Tran-Winkler HJ, Love JF, Gryllos I, Wessels MR (2011) Signal Transduction through CsrRS Confers an Invasive Phenotype in Group A Streptococcus. PLoS Pathog 7: e1002361.
- 23. Cole JN, Barnett TC, Nizet V, Walker MJ (2011) Molecular insight into invasive group A streptococcal disease. Nat Rev Microbiol 9: 724–736.
- 24. Churchward G (2007) The two faces of Janus: virulence gene regulation by CovR/S in group A Streptococci. Mol Microbiol 64: 34–41.
- 25. Lin WJ, Walthers D, Connelly JE, Burnside K, Jewell KA, et al. (2009) Threonine phosphorylation prevents promoter DNA binding of the Group B Streptococcus response regulator CovR. Mol Microbiol 71: 1477–1495.
- 26. Gusa AA, Gao J, Stringer V, Churchward G, Scott JR (2006) Phosphorylation of the group A Streptococcal CovR response regulator causes dimerization and promoter-specific recruitment by RNA polymerase. J Bacteriol 188: 4620–4626.
- 27. Horstmann N, Sahasrabhojane P, Suber B, Kumaraswami M, Olsen RJ, et al. (2011) Distinct single amino acid replacements in the control of virulence regulator protein differentially impact streptococcal pathogenesis. PLoS Pathog 7: e1002311.
- 28. Dalton TL, Scott JR (2004) CovS inactivates CovR and is required for growth under conditions of general stress in Streptococcus pyogenes. J Bacteriol 186: 3928–3937.
- 29. Gryllos I, Grifantini R, Colaprico A, Jiang S, Deforce E, et al. (2007) Mg(2+) signalling defines the group A streptococcal CsrRS (CovRS) regulon. Mol Microbiol 65: 671–683.
- 30. Gryllos I, Tran-Winkler HJ, Cheng MF, Chung H, Bolcome R 3rd, et al. (2008) Induction of group A Streptococcus virulence by a human antimicrobial peptide. Proc Natl Acad Sci U S A 105: 16755–16760.
- 31. Mitrophanov AY, Groisman EA (2008) Signal integration in bacterial two-component regulatory systems. Genes Dev 22: 2601–2611.
- 32. Buelow DR, Raivio TL (2010) Three (and more) component regulatory systems - auxiliary regulators of bacterial histidine kinases. Mol Microbiol 75: 547–566.
- 33. Jung K, Fried L, Behr S, Heermann R (2012) Histidine kinases and response regulators in networks. Curr Opin Microbiol 15: 118–124.
- 34. Rajagopal L, Vo A, Silvestroni A, Rubens CE (2006) Regulation of cytotoxin expression by converging eukaryotic-type and two-component signalling mechanisms in Streptococcus agalactiae. Mol Microbiol 62: 941–957.
- 35. Pei J, Grishin NV (2001) Type II CAAX prenyl endopeptidases belong to a novel superfamily of putative membrane-bound metalloproteases. Trends Biochem Sci 26: 275–277.
- 36. Pei J, Mitchell DA, Dixon JE, Grishin NV (2011) Expansion of type II CAAX proteases reveals evolutionary origin of gamma-secretase subunit APH-1. J Mol Biol 410: 18–26.
- 37. Spellerberg B, Pohl B, Haase G, Martin S, Weber-Heynemann J, et al. (1999) Identification of genetic determinants for the hemolytic activity of Streptococcus agalactiae by ISS1 transposition. J Bacteriol 181: 3212–3219.
- 38. Pritzlaff CA, Chang JC, Kuo SP, Tamura GS, Rubens CE, et al. (2001) Genetic basis for the beta-haemolytic/cytolytic activity of group B Streptococcus. Mol Microbiol 39: 236–247.
- 39. Liu GY, Doran KS, Lawrence T, Turkson N, Puliti M, et al. (2004) Sword and shield: linked group B streptococcal beta-hemolysin/cytolysin and carotenoid pigment function to subvert host phagocyte defense. Proc Natl Acad Sci U S A 101: 14491–14496.
- 40. Forquin MP, Tazi A, Rosa-Fraile M, Poyart C, Trieu-Cuot P, et al. (2007) The putative glycosyltransferase-encoding gene cylJ and the Group B Streptococcus (GBS)-specific gene cylK modulate hemolysin production and virulence of GBS. Infect Immun 75: 2063–2066.
- 41. Costa A, Gupta R, Signorino G, Malara A, Cardile F, et al. (2012) Activation of the NLRP3 inflammasome by group B Streptococci. J Immunol 188: 1953–1960.
- 42. de la Rosa M, Perez M, Carazo C, Pareja L, Peis JI, et al. (1992) New Granada Medium for detection and identification of Group B Streptococci. J Clin Microbiol 30: 1019–1021.
- 43. Glaser P, Rusniok C, Buchrieser C, Chevalier F, Frangeul L, et al. (2002) Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease. Mol Microbiol 45: 1499–1513.
- 44. Tettelin H, Masignani V, Cieslewicz MJ, Donati C, Medini D, et al. (2005) Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial “pan-genome”. Proc Natl Acad Sci U S A 102: 13950–13955.
- 45. Winter-Vann AM, Casey PJ (2005) Post-prenylation-processing enzymes as new targets in oncogenesis. Nat Rev Cancer 5: 405–412.
- 46. Maurer-Stroh S, Eisenhaber F (2005) Refinement and prediction of protein prenylation motifs. Genome Biol 6: R55.
- 47. Plummer LJ, Hildebrandt ER, Porter SB, Rogers VA, McCracken J, et al. (2006) Mutational analysis of the ras converting enzyme reveals a requirement for glutamate and histidine residues. J Biol Chem 281: 4596–4605.
- 48. Karimova G, Pidoux J, Ullmann A, Ladant D (1998) A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proc Natl Acad Sci U S A 95: 5752–5756.
- 49. Laub MT, Goulian M (2007) Specificity in two-component signal transduction pathways. Annu Rev Genet 41: 121–145.
- 50. Huynh TN, Stewart V (2011) Negative control in two-component signal transduction by transmitter phosphatase activity. Mol Microbiol 82: 275–286.
- 51. Huynh TN, Noriega CE, Stewart V (2010) Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX. Proc Natl Acad Sci U S A 107: 21140–21145.
- 52. Trevino J, Perez N, Ramirez-Pena E, Liu Z, Shelburne SA 3rd, et al. (2009) CovS simultaneously activates and inhibits the CovR-mediated repression of distinct subsets of group A Streptococcus virulence factor-encoding genes. Infect Immun 77: 3141–3149.
- 53. Sugareva V, Arlt R, Fiedler T, Riani C, Podbielski A, et al. (2010) Serotype- and strain- dependent contribution of the sensor kinase CovS of the CovRS two-component system to Streptococcus pyogenes pathogenesis. BMC Microbiol 10: 34.
- 54. Matthews EE, Zoonens M, Engelman DM (2006) Dynamic helix interactions in transmembrane signaling. Cell 127: 447–450.
- 55. Hulko M, Berndt F, Gruber M, Linder JU, Truffault V, et al. (2006) The HAMP domain structure implies helix rotation in transmembrane signaling. Cell 126: 929–940.
- 56. Szurmant H, White RA, Hoch JA (2007) Sensor complexes regulating two-component signal transduction. Curr Opin Struct Biol 17: 706–715.
- 57. Goldberg SD, Clinthorne GD, Goulian M, DeGrado WF (2010) Transmembrane polar interactions are required for signaling in the Escherichia coli sensor kinase PhoQ. Proc Natl Acad Sci U S A 107: 8141–8146.
- 58. Eguchi Y, Itou J, Yamane M, Demizu R, Yamato F, et al. (2007) B1500, a small membrane protein, connects the two-component systems EvgS/EvgA and PhoQ/PhoP in Escherichia coli. Proc Natl Acad Sci U S A 104: 18712–18717.
- 59. Lippa AM, Goulian M (2009) Feedback inhibition in the PhoQ/PhoP signaling system by a membrane peptide. PLoS Genet 5: e1000788.
- 60. Gerken H, Charlson ES, Cicirelli EM, Kenney LJ, Misra R (2009) MzrA: a novel modulator of the EnvZ/OmpR two-component regulon. Mol Microbiol 72: 1408–1422.
- 61. Szurmant H, Mohan MA, Imus PM, Hoch JA (2007) YycH and YycI interact to regulate the essential YycFG two-component system in Bacillus subtilis. J Bacteriol 189: 3280–3289.
- 62. Szurmant H, Bu L, Brooks CL 3rd, Hoch JA (2008) An essential sensor histidine kinase controlled by transmembrane helix interactions with its auxiliary proteins. Proc Natl Acad Sci U S A 105: 5891–5896.
- 63. Dintner S, Staron A, Berchtold E, Petri T, Mascher T, et al. (2011) Coevolution of ABC Transporters and Two-Component Regulatory Systems as Resistance Modules against Antimicrobial Peptides in Firmicutes Bacteria. J Bacteriol 193: 3851–3862.
- 64. Mascher T (2006) Intramembrane-sensing histidine kinases: a new family of cell envelope stress sensors in Firmicutes bacteria. FEMS Microbiol Lett 264: 133–144.
- 65. Goodman AL, Merighi M, Hyodo M, Ventre I, Filloux A, et al. (2009) Direct interaction between sensor kinase proteins mediates acute and chronic disease phenotypes in a bacterial pathogen. Genes Dev 23: 249–259.
- 66. Rajagopal L, Clancy A, Rubens CE (2003) A eukaryotic type serine/threonine kinase and phosphatase in Streptococcus agalactiae reversibly phosphorylate an inorganic pyrophosphatase and affect growth, cell segregation, and virulence. J Biol Chem 278: 14429–14441.
- 67. Rajagopal L, Vo A, Silvestroni A, Rubens CE (2005) Regulation of purine biosynthesis by a eukaryotic-type kinase in Streptococcus agalactiae. Mol Microbiol 56: 1329–1346.
- 68. Silvestroni A, Jewell KA, Lin WJ, Connelly JE, Ivancic MM, et al. (2009) Identification of serine/threonine kinase substrates in the human pathogen group B Streptococcus. J Proteome Res 8: 2563–2574.
- 69. Burnside K, Lembo A, Harrell MI, Gurney M, Xue L, et al. (2011) Serine/threonine phosphatase Stp1 mediates post-transcriptional regulation of hemolysin, autolysis, and virulence of group B Streptococcus. J Biol Chem 286: 44197–44210.
- 70. Beilharz K, Novakova L, Fadda D, Branny P, Massidda O, et al. (2012) Control of cell division in Streptococcus pneumoniae by the conserved Ser/Thr protein kinase StkP. Proc Natl Acad Sci U S A 109: E905–913.
- 71. Pereira SF, Goss L, Dworkin J (2011) Eukaryote-like serine/threonine kinases and phosphatases in bacteria. Microbiol Mol Biol Rev 75: 192–212.
- 72. Shah IM, Laaberki MH, Popham DL, Dworkin J (2008) A eukaryotic-like Ser/Thr kinase signals bacteria to exit dormancy in response to peptidoglycan fragments. Cell 135: 486–496.
- 73. Tazi A, Disson O, Bellais S, Bouaboud A, Dmytruk N, et al. (2010) The surface protein HvgA mediates group B streptococcus hypervirulence and meningeal tropism in neonates. J Exp Med 207: 2313–2322.
- 74. Bebien M, Hensler ME, Davanture S, Hsu LC, Karin M, et al. (2012) The Pore-Forming Toxin beta hemolysin/cytolysin Triggers p38 MAPK-Dependent IL-10 Production in Macrophages and Inhibits Innate Immunity. PLoS Pathog 8: e1002812.
- 75. Price CT, Al-Quadan T, Santic M, Jones SC, Abu Kwaik Y (2010) Exploitation of conserved eukaryotic host cell farnesylation machinery by an F-box effector of Legionella pneumophila. J Exp Med 207: 1713–1726.
- 76. Reinicke AT, Hutchinson JL, Magee AI, Mastroeni P, Trowsdale J, et al. (2005) A Salmonella typhimurium effector protein SifA is modified by host cell prenylation and S-acylation machinery. J Biol Chem 280: 14620–14627.
- 77. Al-Quadan T, Price CT, London N, Schueler-Furman O, AbuKwaik Y (2011) Anchoring of bacterial effectors to host membranes through host-mediated lipidation by prenylation: a common paradigm. Trends Microbiol 19: 573–579.
- 78. Grundling A, Missiakas DM, Schneewind O (2006) Staphylococcus aureus mutants with increased lysostaphin resistance. J Bacteriol 188: 6286–6297.
- 79. Frankel MB, Wojcik BM, DeDent AC, Missiakas DM, Schneewind O (2010) ABI domain-containing proteins contribute to surface protein display and cell division in Staphylococcus aureus. Mol Microbiol 78: 238–252.
- 80. Chopin MC, Chopin A, Bidnenko E (2005) Phage abortive infection in lactococci: variations on a theme. Curr Opin Microbiol 8: 473–479.
- 81. Kjos M, Snipen L, Salehian Z, Nes IF, Diep DB (2010) The Abi proteins and their involvement in bacteriocin self-immunity. J Bacteriol 192: 2068–2076.
- 82. Gonzalez-Pastor JE, Hobbs EC, Losick R (2003) Cannibalism by sporulating bacteria. Science 301: 510–513.
- 83. Lee SW, Mitchell DA, Markley AL, Hensler ME, Gonzalez D, et al. (2008) Discovery of a widely distributed toxin biosynthetic gene cluster. Proc Natl Acad Sci U S A 105: 5879–5884.
- 84. Molloy EM, Cotter PD, Hill C, Mitchell DA, Ross RP (2011) Streptolysin S-like virulence factors: the continuing sagA. Nat Rev Microbiol 9: 670–681.
- 85. Nizet V, Beall B, Bast DJ, Datta V, Kilburn L, et al. (2000) Genetic locus for streptolysin S production by Group A Streptococcus. Infect Immun 68: 4245–4254.
- 86. Datta V, Myskowski SM, Kwinn LA, Chiem DN, Varki N, et al. (2005) Mutational analysis of the group A streptococcal operon encoding streptolysin S and its virulence role in invasive infection. Mol Microbiol 56: 681–695.
- 87. Heckman KL, Pease LR (2007) Gene splicing and mutagenesis by PCR-driven overlap extension. Nat Protoc 2: 924–932.
- 88. Biswas I, Gruss A, Ehrlich SD, Maguin E (1993) High-efficiency gene inactivation and replacement system for gram-positive bacteria. J Bacteriol 175: 3628–3635.
- 89. Lalioui L, Pellegrini E, Dramsi S, Baptista M, Bourgeois N, et al. (2005) The SrtA sortase of Streptococcus agalactiae is required for cell wall anchoring of proteins containing the LPXTG motif, for adhesion to epithelial cells, and for colonization of the mouse intestine. Infect Immun 73: 3342–3350.
- 90. Dramsi S, Dubrac S, Konto-Ghiorghi Y, Da Cunha V, Couve E, et al. (2012) Rga, a RofA-Like Regulator, Is the Major Transcriptional Activator of the PI-2a Pilus in Streptococcus agalactiae. Microb Drug Resist 18: 286–297.