Experimental obstacles have impeded our ability to study prion transmission within and, more particularly, between species. Here, we used cervid prion protein expressed in brain extracts of transgenic mice, referred to as Tg(CerPrP), as a substrate for in vitro generation of chronic wasting disease (CWD) prions by protein misfolding cyclic amplification (PMCA). Characterization of this infectivity in Tg(CerPrP) mice demonstrated that serial PMCA resulted in the high fidelity amplification of CWD prions with apparently unaltered properties. Using similar methods to amplify mouse RML prions and characterize the resulting novel cervid prions, we show that serial PMCA abrogated a transmission barrier that required several hundred days of adaptation and subsequent stabilization in Tg(CerPrP) mice. While both approaches produced cervid prions with characteristics distinct from CWD, the subtly different properties of the resulting individual prion isolates indicated that adaptation of mouse RML prions generated multiple strains following inter-species transmission. Our studies demonstrate that combined transgenic mouse and PMCA approaches not only expedite intra- and inter-species prion transmission, but also provide a facile means of generating and characterizing novel prion strains.
Prions are unique pathogens that result from conversion of a normal host-encoded prion protein, PrPC, into a self-propagating, disease-associated conformation, referred to as PrPSc. An important aspect of prion diseases is their transmissibility, frequently as epidemics. The contagious transmission of chronic wasting disease (CWD) of deer and elk is of particular concern. The elements governing prion transmission between species, including the influence of agent strain properties, remain enigmatic, in large part because of considerable difficulties associated with experimental manipulation of prions. The aim of this study was to evaluate the feasibility of expediting studies of intra- and inter-species prion transmission. We made use of transgenic mice as a source of deer prion protein for the production of CWD prions by protein misfolding cyclic amplification (PMCA). Characterization of infectivity in the same transgenic mice demonstrated that PMCA results in the efficient amplification of CWD prions with unaltered strain characteristics. Also, whereas adaptation of mouse prions to form novel cervid prions required several hundred days and subsequent stabilization in transgenic mice, we show that PMCA rapidly abrogated this inter-species transmission barrier. Our results indicate that PMCA can be used to replace the process of prion strain adaptation and selection occurring in vivo.
Citation: Green KM, Castilla J, Seward TS, Napier DL, Jewell JE, Soto C, et al. (2008) Accelerated High Fidelity Prion Amplification Within and Across Prion Species Barriers. PLoS Pathog 4(8): e1000139. https://doi.org/10.1371/journal.ppat.1000139
Editor: Neil Mabbott, University of Edinburgh, United Kingdom
Received: February 19, 2008; Accepted: August 1, 2008; Published: August 29, 2008
Copyright: © 2008 Green et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grants from the US Public Health Service, namely 2RO1 NS040334-04 from the National Institute of Neurological Disorders and Stroke, and N01-AI-25491 from the National Institute of Allergy and Infectious Diseases. K.M.G. was supported in part by funds from the T32 AI49795 Training Program in Microbial Pathogenesis. None of the sponsors or funders played any role in the design and conduct of the study, in the collection, analysis, and interpretation of the data, or in the preparation, review, or approval of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Prion diseases are transmissible, fatal, and incurable neurodegenerative disorders of the central nervous system (CNS) that include bovine spongiform encephalopathy (BSE), ovine scrapie, chronic wasting disease (CWD) of cervids and human Creutzfeldt-Jakob disease (CJD). While inoculation of diseased brain material into individuals of the same species typically reproduces disease, studies of prion transmissions are complicated by prolonged, clinically silent incubation periods lasting months to years. Inter-species prion transmission is generally an even less efficient process, a phenomenon referred to as the species barrier .
While studies in transgenic (Tg) mice – and cell-free systems , demonstrated the influence of PrP primary structure on prion transmission, agent strain properties are an equally important determinant. Thus, the time between inoculation and onset of clinical signs, referred to as the incubation time, is a parameter that varies between strains. Different strains may also induce distinct clinical signs in inoculated animals. Neuropathologically, strains are distinguished by reproducible differences in the distribution of spongiform degeneration of the cerebral grey matter, and by the deposition of PrPSc, occasionally in the form of amyloid plaques. While the different strain properties of conventional pathogens are genomically encoded, it is less clear how multiple disease phenotypes can be accommodated in the context of a ‘protein only’ mechanism of pathogenesis where the infectious agent lacks nucleic acid. Numerous studies suggest that strain diversity is enciphered in the higher order structure of PrPSc –; accordingly, the biochemical properties of PrPSc have also been used as a means of typing prion isolates ,.
While prion strain properties are stably maintained upon passage within a particular species, inter-species prion transmission may result in the acquisition of new strain properties, the most profound of which may be host range alteration ,. Thus, the species tropism of novel prion strains currently cannot be predicted. A powerful demonstration of the unpredictable influence of prion strains on species barriers is highlighted in the case of BSE. Cattle feed derived from rendered meat and bone meal which was contaminated with prions, possibly originating from scrapie-infected sheep, is the suspected origin of BSE . BSE-related prion diseases were subsequently identified in domestic and captive wild cats , and exotic ungulates. The recognition that a variant of CJD (vCJD) is caused by the BSE prion strain – raised major public health concerns. Like BSE, the origin of transmissible mink encephalopathy (TME) of ranch-raised mink, is thought to be prion-contaminated feed .
Recent years have witnessed the emergence of additional novel mammalian prion strains. Atypical scrapie is a recently-recognized and surprisingly prevalent prion disease of sheep of unknown origin and host-range. First reported in Norwegian sheep in 2003 and referred to as Nor98 , atypical scrapie appears to be a single, unique scrapie strain , infecting sheep with PRNP genotypes usually associated with resistance to classical scrapie. The increasing geographic range, contagious transmission, uncertain strain prevalence, and environmental persistence of CWD are also of concern. Uncontrolled prion dissemination in wild cervid populations brings into question the risk of transmission to other species, for example via shared grazing of CWD-contaminated rangeland. Insights into the factors controlling prion transmission and host-range adaptation are clearly of paramount importance for containing further prion epidemics.
The aim of this study was to evaluate the feasibility of expediting studies of intra-, and inter-species prion transmission by combining the resources of protein misfolding cyclic amplification (PMCA)  with Tg mouse models of prion disease. During PMCA, the normal form of PrP, referred to as PrPC, is converted into protease-resistant PrP using small amounts of infectious PrPSc. Continued recruitment and conversion of PrPC by PrPSc is accomplished by sonication in a process analogous to amplification of DNA by the polymerase chain reaction . We previously showed that Tg mice expressing PrP from mule deer, referred to as Tg(CerPrP) mice, are susceptible to prions from deer and elk dying of CWD –. Several other groups subsequently confirmed these observations using similar mouse models –. Here we used cervid PrPC (CerPrPC) expressed in the brains of Tg mice for the generation of CWD prions by PMCA. Using Tg(CerPrP) mice to characterize this in vitro-generated infectivity we demonstrate that PMCA results in the high fidelity amplification of CWD prions with apparently unaltered strain properties. In addition, while adaptation of mouse prions to form novel cervid prions required several hundred days in Tg(CerPrP) mice, we show that PMCA abrogated this barrier to prion transmission resulting in the rapid generation of novel cervid prions with similar properties.
Serial PMCA of CWD-seeded CerPrPC from Tg(CerPrP) mice results in high fidelity CWD prion replication
A PMCA reaction was established using a CWD prion seed in a 10% brain homogenate from diseased mule deer 04-22412, diluted 10-fold into 10% brain homogenate prepared from perfused Tg(CerPrP)1536+/− mice . Following a round of PMCA consisting of alternating periods of sonication and incubation for 36 cycles, the product, which contained amplified protease-resistant CerPrP (Fig. 1A), was diluted 10-fold into another reaction containing CerPrPC from Tg(CerPrP)1536+/− mouse brain homogenate for a further round of PMCA. This process of serial PMCA was repeated for 22 rounds. In accordance with previous studies using the experimentally-adapted hamster scrapie isolate 263K ,, and the experimentally-adapted Chandler scrapie isolate , protease-resistant CerPrP was amplified to high levels during each round of serial PMCA (Fig. 1A and C). In contrast, after 10 rounds of serial PMCA of six duplicated samples of a healthy Tg(CerPrP)1536+/− brain extract, no protease-resistant PrP was produced in the absence of prion seeds (data not shown).
A: Serial PMCA of 04-22412 CWD using Tg(CerPrP)1536+/− brain homogenate. CWD prions in a 10% brain homogenate of diseased mule deer 04-22412 were diluted 10-fold into 10% brain homogenate from perfused Tg(CerPrP)1536+/− mice. Following a round of PMCA, the sample, containing amplified protease-resistant CerPrP, was diluted 10-fold into 10% brain homogenate from perfused Tg(CerPrP)1536+/− mice for a further round of PMCA. This process of serial PMCA was repeated for 22 rounds. PK-treated samples from each of the first 10 rounds were analyzed by Western blotting. In the final lane, a sample from Tg brain homogenate without PK treatment was loaded. B: Serial PMCA of RML using Tg(CerPrP)1536+/− brain homogenate. Mouse RML prions in a 10% brain homogenate from a diseased wild type FVB mouse were diluted 10-fold into 10% brain homogenate from perfused Tg(CerPrP)1536+/− mice. Serial PMCA was repeated for 22 rounds. PK-treated samples from each of the first 7 rounds were analyzed by Western blotting. The unamplified RML seed that produced protease-resistant PrP following PMCA in round 1 was loaded in the first lane, while a sample from Tg brain homogenate without PK treatment was loaded in the final lane. C: Western blot quantification of protease-resistant PrP in inocula used to challenge Tg(CerPrP)1536+/− mice. Samples were PK-treated as indicated. Ratios indicate the fold dilution of the original preparation. In the final lane, a sample from CWD brain homogenate without PK treatment was loaded.
To ascertain whether this process resulted in the in vitro amplification of CWD prions, Tg(CerPrP)1536+/− mice were intracerebrally challenged with the product of 22 rounds of serial PMCA (Fig. 1C). A separate cohort was inoculated with a 1% brain homogenate of the CWD-infected 04-22412 mule deer isolate that was the seed for the initial round of PMCA. In both cases, Tg(CerPrP)1536+/− mice were inoculated with preparations containing similar amounts of protease-resistant CerPrP, as determined by Western blot analysis (Fig. 1C). Serial PMCA reactions initially seeded with 04-22412 CWD prions but using Prnp0/0 knockout instead of Tg(CerPrP)1536+/− mouse brain homogenate were also performed in parallel. A cohort of Tg(CerPrP)1536+/− mice inoculated with this material after 22 rounds of serial PMCA served as negative controls to show that the original CWD inoculum was not detectable.
All Tg(CerPrP)1536+/− mice (n = 6) inoculated with material derived from serial PMCA of 04-22412 CWD prions using CerPrPC from the brains of Tg(CerPrP)1536+/− mice developed disease with a mean incubation time of 263±28 (mean±standard error) days (d) (Fig. 2). Consistent with previous results –32, CWD prions from the brain of diseased 04-22412 mule deer also induced disease in Tg(CerPrP)1536+/− mice (n = 6) with an incubation time of 284±22 d. The clinical signs that accompanied prion disease were identical in both cases, and included truncal ataxia and slowed movement, increased tone of the tail, dorsal kyphosis, head bobbing or tilting, and roughened coat. Confirming that prion infectivity produced by 22 rounds of serial PMCA was unrelated to persistence of the initial 04-22412 CWD prion seed, no disease was registered in Tg(CerPrP)1536+/− mice inoculated with preparations from the negative control reaction in which 04-22412 CWD prions were seeded into Prnp0/0 brain homogenate followed by 22 rounds serial PMCA (Fig. 2). Detection of CerPrPSc in the brains of diseased Tg(CerPrP)1536+/− mice by Western blotting (Fig. 3A), histoblotting (Fig. 4), and immunohistochemical analysis (Fig. 5B–E) confirmed that the clinical signs following inoculation with CWD prions or the amplified samples were the consequence of prion disease. Collectively these results demonstrate that serial PMCA resulted in the efficient in vitro production of infectious CWD prions. We therefore refer to infectivity in the amplified samples as PMCA CWD prions.
Each symbol represents an individual mouse. Closed symbols indicate diseased mice and open symbols indicate asymptomatic mice. Green symbols indicate prions originating from CWD; blue symbols indicate prions originating from RML; circles indicate in vivo-derived prions; squares indicate PMCA-derived prions. The blue circle surrounded by the red square signifies mouse #4827 that was the origin of Cer/RML-4827 prions, while the green and blue squares signify mice #5302 and #4825 respectively, the brains of which were analyzed by histoblotting. Incubation times are expressed as the mean±standard error of the mean; listed in parenthesis is number of diseased mice/ number of mice inoculated.
A: Western blot showing accumulation of CerPrPSc in the brains of diseased Tg(CerPrP)1536+/− mice inoculated with CWD or PMCA CWD prions. B: Western blot comparison of PrPSc in the brains of diseased FVB and Tg(CerPrP)1536+/− mice (Tg1536) inoculated with mouse RML prions. C. Western blot showing CerPrPSc accumulation in Tg(CerPrP)1536+/− mice infected with mouse RML prions, Cer/RML-4827, and PMCA Cer/RML prions. D: Ratio of three protease-resistant PrP glycoforms produced in the brains of diseased Tg(CerPrP)1536+/− mice or FVB mice. Data points represent the mean relative proportions of di-, mono-, and un-glycosylated PrP as a percentage derived from densitometric quantification of PrPSc in brains of three individual diseased mice in each case. Error bars indicate the standard error of the mean which, in some cases, was smaller than the symbols used. Samples for Western blot analysis were either untreated (−) or treated (+) with PK and 50 µg and 100 µg of total protein was loaded for untreated and treated samples respectively. The positions of protein molecular mass markers at 37, 25 and 20 kDa (from top to bottom) are shown.
PK-treated (+) or untreated (−) histoblotted coronal sections, as indicated, of terminally sick Tg(CerPrP)1536+/− mice inoculated with A, naturally occurring CWD prions from mule deer isolate 04-22412 or B, PMCA-derived CWD prions. Histoblots were stained with Hum-P anti-PrP recombinant Fab followed by alkaline phosphatase-conjugated goat anti-human secondary antibody.
A, B, and D are sections through the hippocampus of non-diseased or diseased Tg(CerPrP)1536+/− mice; section C is from the cerebral cortex. A, absence of spongiform pathology and immunohistochemically-reactive PrP in the hippocampus of an asymptomatic PBS-inoculated Tg(CerPrP)1536+/− mouse; B, accumulation of plaques in the hippocampus of diseased Tg(CerPrP)1536+/− mouse inoculated with naturally occurring 04-22412 CWD prions; C, accumulation of plaques in the cerebral cortex of a diseased Tg(CerPrP)1536+/− mouse inoculated with naturally occurring 04-22412 CWD prions; D, accumulation of plaques in the hippocampus of diseased Tg(CerPrP)1536+/− mouse inoculated with PMCA CWD prions; E, high magnification of a large plaque aggregate rimmed by vacuoles; F, absence of spongiform pathology and immunohistochemically reactive PrP in the medulla of asymptomatic PBS-inoculated Tg(CerPrP) 1536+/− mouse; G, diffuse PrP accumulation in the medulla of diseased Tg(CerPrP)1536+/− mouse #5297 inoculated with mouse RML prions; H, high magnification of a section thought the hippocampus of diseased Tg(CerPrP)1536+/− mouse #5300 showing PrP accumulation in small plaques; I, diffuse PrP accumulation in the medulla of a diseased Tg(CerPrP)1536+/− mouse inoculated with PMCA Cer/RML prions. Hematoxylin was used as counterstain. Bar = 100 µm in A–D; Bar = 50 µm in E–I.
Concordant strain properties of PMCA-derived and naturally occurring CWD prions
In order to fully evaluate the biochemical and neuropathological characteristics of disease induced by both inocula, we performed detailed comparative studies of the brains of mice infected with naturally occurring and PMCA CWD prions. The similar incubation times of naturally occurring and PMCA CWD prions (Fig. 2) raised the possibility that the strain properties of the 04-22412 CWD prion isolate were maintained during serial PMCA. Consistent with this notion, the electrophoretic mobilities (Fig. 3A) and glycosylation profiles of CerPrPSc produced in the brains of Tg(CerPrP)1536+/− mice inoculated with both preparations were similar (Fig. 3D).
Assessment of the neuroanatomical distribution of PrPSc by histoblotting is another parameter that has been used to characterize prion strains ,,32,41,42. The appearance and distribution of CerPrPSc throughout histoblotted brain sections of diseased Tg(CerPrP)1536+/− mice infected with 04-22412 CWD (Fig. 4A) or PMCA CWD prions (Fig. 4B) were similar (n = 1 in each group). Markedly punctate accumulations of CerPrPSc were present in histoblotted brain sections of mice infected with both naturally occurring and PMCA CWD prions, either prior to, or following treatment with proteinase K (PK). CerPrPSc-containing aggregates often coalesced into larger immunoreactive structures. Similar aggregation and distribution of CerPrPSc has been reported in Tg(CerPrP)1536+/− mice infected with various naturally occurring deer and elk CWD isolates ,.
The accumulation of CerPrPSc in plaques was confirmed by immunohistochemical analyses of brains from diseased Tg(CerPrP)1536+/− mice infected with naturally occurring CWD prions (Fig. 5B and C) and PMCA CWD prions (Fig. 5D and E). The distribution of immunoreactive plaques and accompanying spongiform degeneration was similar in both cases (Fig. 5B–E), with plaques often coalescing into larger structures frequently bordered by vacuoles (Fig. 5E).
Previous studies showed the unfolding characteristics of PrPSc to be a sensitive and quantitative means of assessing strain-dependent differences in PrPSc conformation ,,,. We therefore determined the relative stabilities of CerPrPSc in the brains of Tg(CerPrP)1536+/− mice infected with PMCA-generated or in vivo-derived CWD prions. Brian extracts were treated with increasing concentrations of guanidine hydrochloride (GdnHCl), followed by PK digestion and analysis of residual CerPrPSc by Western blotting. When plotted, the mean amounts of PK-resistant PrP in the brains of three diseased Tg(CerPrP)1536+/− mice at each concentration of denaturant, formed sigmoidal curves. The transition point at the concentration where half the CerPrPSc in the samples was denatured is referred to as the mean GdnHCl1/2 value. Similar denaturation properties and mean GdnHCl1/2 values indicated comparable CerPrPSc stabilities following infection with 04-22412 CWD and PMCA CWD prions (Fig. 6A and B).
In A and C, densitometric analysis of immunoblots shows the percentage of protease-resistant CerPrPSc as a function of GdnHCl concentration. The sigmoidal dose-response was plotted using a four-parameter algorithm and non-linear least-square fit. Each point shown is the mean value derived from densitometric quantification of PK-resistant PrP in three diseased Tg(CerPrP)1536+/− mouse brain extracts in each study group. Error bars indicate the standard error of the mean which, in some cases, was smaller than the size of the symbols used to indicate the mean. A, Tg(CerPrP)1536+/− mice inoculated with 04-22412 CWD prions (green filled circles), or PMCA-derived CWD prions (green filled squares); C, Tg(CerPrP)1536+/− mice inoculated with Cer/RML-4827 prions (blue filled circles), or PMCA Cer/RML prions (blue filled squares). For comparison, the conformational stability of MoPrPSc in the brains of wild type FVB mice infected with RML prions is shown (black diamonds). In B and D, representative immunoblots of protease-resistant PrP following PK treatment are shown. The mean GdnHCl1/2 value, representing the concentration at which half the PrPSc in each series was denatured, is also shown.
Collectively, the concordant clinical and histological profiles of Tg(CerPrP)1536+/− mice infected with naturally occurring and PMCA-derived CWD prions, as well as the similar biochemical properties of the resulting CerPrPSc, indicate that the characteristics of 04-22412 CWD prions were maintained during serial PMCA.
Interspecies transmission of mouse RML prions in Tg(CerPrP) mice results in variable CNS disease
While deer and elk CWD prions propagated efficiently in Tg(CerPrP)1536+/− mice, with 100% rates of transmission and mean incubation times ranging from ∼225 to 270 d, our previous studies showed that Tg(CerPrP)1536+/− mice remained free of prion disease for >1 year after infection with mouse RML prions . To fully characterize the extent of this transmission barrier, we challenged additional Tg(CerPrP)1536+/− mice with mouse RML prions and extended our observations beyond one year. While all RML-inoculated Tg(CerPrP)1536+/− mice (n = 9) eventually developed clinical signs, the time to disease onset was protracted and highly variable (mean incubation time, 489±22 d; range of disease onset ∼400 to 590 d) (Fig. 2). In contrast to the predominantly monoglycosylated profile of mouse PrPSc in the brains of RML infected wild type FVB mice (Figs. 1C, 3B and 3D), CerPrPSc produced in the brains of RML infected Tg(CerPrP)1536+/− mice was predominantly diglycosylated (Fig. 3B, C and D). This suggested that adaptation of mouse RML prions occurred following transit across a species barrier in mice expressing CerPrPC.
Histoblot analysis revealed variable distribution and aggregation of CerPrPSc in the CNS of two diseased Tg(CerPrP)1536+/− mice infected with mouse RML prions (Fig. 7). The deposition of CerPrPSc in the brain of Tg(CerPrP)1536+/− mouse #4825, which developed disease 512 d after infection, was widespread and diffuse. In contrast, CerPrPSc in the brain of Tg(CerPrP)1536+/− mouse #5302, which developed disease after 488 d, accumulated in small, discrete plaques. The punctate staining observed in mouse #5302 differed from the CerPrPSc aggregates in Tg(CerPrP)1536+/− mice infected with naturally occurring or PMCA CWD prions (Fig. 4), which were deposited in different brain regions, and frequently coalesced into larger immunoreactive structures. Immunohistochemical analysis of additional RML-infected Tg(CerPrP)1536+/− mouse brains confirmed that CerPrPSc deposition and aggregation varied between animals. While diffuse CerPrPSc staining characterized the CNS of animal #5297 (Fig. 5G), CerPrPSc accumulated in small plaques in the CNS of animal #5300 (Fig. 5H).
PK-treated histoblotted coronal sections though, from top to bottom, the region of the septum, hippocampus, anterior midbrain, posterior midbrain, pons, and oblongata from diseased Tg(CerPrP)1536+/− mice inoculated with mouse RML prions, Cer/RML-4827, or PMCA Cer/RML prions. Sections were prepared from two different Tg(CerPrP)1536+/− mice inoculated with mouse RML prions (#4825 and #5302) and two different Tg(CerPrP)1536+/− mice inoculated with PMCA Cer/RML prions (#5294 and #5295). Lateral areas of the posterior midbrain section of mouse #4825 were lost during tissue processing. Histoblots of sections through the anterior and posterior midbrain from an asymptomatic age-matched Tg(CerPrP)1536+/− mouse inoculated with PBS, shown to the left, demonstrate the specificity of immunostaining with Hum-P anti-PrP recombinant Fab.
Abrogation of the transmission barrier to mouse prions in Tg(CerPrP) mice produces novel cervid-adapted prions with properties distinct from CWD
The brain of Tg(CerPrP)1536+/− mouse #4827 that developed disease 394 d after infection with mouse RML prions was prepared for serial transmission studies. Infectivity in the brain of this mouse, referred to as Cer/RML-4827 prions, induced disease in Tg(CerPrP)1536+/− mice (n = 8) with a mean incubation time of 148±5 d (Fig. 2). This substantial reduction in time to onset of disease, as well as the narrow range of incubation times on second passage, is characteristic of prion adaptation following transit across a species barrier. The diglycosylated CerPrPSc pattern that characterized infection of Tg(CerPrP)1536+/− mice with mouse RML prions was maintained upon passage of Cer/RML-4827 prions to Tg(CerPrP)1536+/− mice (Fig. 3C and D). Histoblot analysis of a recipient Tg(CerPrP)1536+/− mouse brain #7263 showed that infection with Cer/RML-4827 prions was characterized by diffuse rather than punctate CerPrPSc distribution (Fig. 7).
The denaturation profile of CerPrPSc in the brains of Tg(CerPrP)1536+/− mice infected with Cer/RML-4827 prions differed from mouse PrPSc in the brains of RML-infected FVB mice (mean GdnHCl1/2 values of 1.27 M and 1.57 M respectively, Fig. 6C and D). This conformational difference is consistent with adaptation of mouse RML prions following replication in Tg(CerPrP)1536+/− mice. Moreover, the denaturation profile of CerPrPSc produced in response to infection with Cer/RML-4827 prions was considerably different from the profiles of CerPrPSc produced following infection with naturally occurring or PMCA-derived CWD prions (mean GdnHCl1/2 values of 1.27 M and 2.7–2.8 M respectively, Fig. 6A and B). This indicated that adaptation of mouse RML prions in Tg(CerPrP)1536+/− mice resulted in the formation of novel cervid prions with a conformation distinct from CWD.
Formation and characterization of PMCA-derived cervid adapted RML prions
We investigated whether PMCA could abrogate the barrier to mouse RML prion transmission that was ultimately breached following adaptation in Tg(CerPrP)1536+/− mice. Brain homogenates from uninfected Tg(CerPrP)1536+/− mice were seeded with mouse RML prions and 22 rounds of serial PMCA were performed as before. Protease-resistant CerPrP was amplified to high levels during each round of serial PMCA, culminating in 22 rounds (Fig. 1B and C). The change in PrPSc glycoform pattern that occurred following passage of mouse RML prions from wild type to Tg(CerPrP)1536+/− mice (Fig. 3B and D) also appeared to be a feature of RML adaptation during serial PMCA, with protease-resistant CerPrP becoming predominantly diglycosylated at round 2 and thereafter (Fig. 1B). No protease-resistant PrP was produced in the absence of prion seeds after 10 rounds of serial PMCA of six duplicated samples of a healthy Tg(CerPrP)1536+/− brain extract (data not shown).
Remarkably, inoculation of Tg(CerPrP)1536+/− mice with this PMCA-adapted material, rapidly induced disease in all inoculated Tg(CerPrP)1536+/− mice (n = 7). The 143±6 d mean incubation time was strikingly similar to the ∼150 d mean incubation time of Cer/RML-4827 prions that resulted from adaptation of RML prions following replication in Tg(CerPrP)1536+/− mice (Fig. 2). This indicated that the barrier to inter-species transmission of mouse RML prions, which requires several hundred days of adaptation in Tg(CerPrP) mice and stabilization on serial passage, can be directly bypassed by serial PMCA of RML using CerPrPC from Tg mouse brain. We therefore refer to infectivity in this amplified sample as PMCA Cer/RML prions.
Using Western blotting, conformational stability assays, and histoblotting we characterized the properties of CerPrPSc produced in the brains of diseased Tg(CerPrP)1536+/− mice infected with PMCA Cer/RML prions. The diglycosylated profile of CerPrPSc in the PMCA Cer/RML inoculum (Fig. 1C) was maintained in the brains of diseased Tg(CerPrP)1536+/− mice (Fig. 3C and D). The denaturation profile and mean GdnHCl1/2 value of CerPrPSc in the brains of Tg(CerPrP)1536+/− mice infected with PMCA Cer/RML prions was equivalent to Cer/RML-4827, but different from RML in wild type FVB mice or from naturally occurring or PMCA-derived CWD prions in Tg(CerPrP)1536+/− mice. This indicated that, similar to the adaptation of RML in Tg(CerPrP)1536+/− mice, serial PMCA resulted in adaptation of mouse RML prions to produce novel cervid prions with a CerPrPSc conformation distinct CWD prions. Histoblotting revealed a consistent pattern of CerPrPSc distribution in the brains of two Tg(CerPrP)1536+/− mice infected with PMCA Cer/RML prions (Fig. 7). The diffuse CerPrPSc deposition in the brains of two such mice, referred to as #5294 and #5295, was distinct from the small punctate staining pattern in the CNS of Tg(CerPrP)1536+/− #5302 mouse infected with RML, or the large CerPrPSc deposits in the CNS of Tg(CerPrP)1536+/− mice infected with CWD prions (Fig. 4). Comparison of the histoblot patterns in #5294 and #5295 mice with Tg(CerPrP)1536+/− mouse #7263 that was infected with Cer/RML-4827 prions also revealed subtle differences in the regional distribution of CerPrPSc with, for example, relative sparing of the corpus callosum in the #5294 and #5295 mice infected with PMCA Cer/RML prions (Fig. 7). These differences in CerPrPSc distribution suggest that subtle strain differences distinguish Cer/RML-4827 and PMCA Cer/RML prions.
Collectively, the similar rapid incubation times of PMCA Cer/RML and Cer/RML-4827 prions, and the distinctive properties of the resulting CerPrPSc, demonstrate that both processes produced novel cervid prions with biological properties distinct from CWD. Our findings therefore indicate that serial PMCA substituted for the long-term process of RML prion adaptation in Tg(CerPrP)1536+/− mice. Nonetheless, our histoblotting and immunohistochemical analyses show that adaptation of RML prions in Tg(CerPrP)1536+/− mice resulted in the formation of at least two distinct isolates, and that PMCA adaptation likely resulted in a third. These observations indicate that multiple isolates with different strain properties may be produced during the process of prion adaptation following inter-species transmission.
Accelerated high fidelity intra-species prion amplification
The studies reported here are significant in showing that PrPSc and CWD prion infectivity from diseased deer brain are faithfully reproduced in vitro by PMCA using CerPrPC from the brains of Tg(CerPrP)1536+/− mice as the substrate for amplification. They extend previous reports using Tg(CerPrP)1536+/− mice  by showing that PMCA-derived CWD prions induce disease and the production of CerPrPSc in Tg(CerPrP) mice as efficiently as prions isolated from the CNS of deer with CWD.
Tg mice represent a convenient, controlled source of PrPC for PMCA with significant advantages over PrPC from animals or humans. Any form of transgene-derived PrP, either mutated or PrPC from different species, can be readily overexpressed on a Prnp0/0 background, and the brains of Tg mice can be appropriately prepared for use in PMCA. Underscoring this concept, brain homogenate from Tg mice expressing human PrP was recently used to amplify PrPSc from the brains of variant CJD patients by PMCA . As we show here, Tg mice also provide a crucial additional resource in which to fully characterize the biological properties of PMCA-derived prions. These studies raise the prospect of using PMCA and Tg mice expressing mutant and wild type PrP, and polymorphic variants thereof, from cervids, humans, cattle, sheep, rodents, horses, and other mammals, to characterize the strain and host-range properties of naturally occurring prion strains.
Our studies not only reassuringly support previous demonstrations that serial PMCA reproduces experimentally-adapted scrapie 263K prions ,, but also demonstrate (to our knowledge for the first time) cell-free amplification of naturally occurring prion infectivity. Previous serial PMCA of the 263K isolate resulted in the generation of prions with apparently lower specific infectivity than brain-derived infectious material ,. Here we show that the mean incubation times of naturally occurring 04-22412 CWD and PMCA-derived CWD prion preparations in Tg(CerPrP)1536+/− mice were comparable. Since each inoculum comprised similar amounts of CerPrPSc, this suggests that equivalent levels of CWD prion infectivity were present in each case. While the reason for the discrepant behavior of 263K and 04-22412 CWD prions is unknown, the suggestion that PMCA may have generated a different prion strain after repeated in vitro amplification of 263K prions  appears unlikely in the case of PMCA of CWD prions.
To analyze and compare the strain properties of PMCA CWD and naturally occurring CWD prions we analyzed several independent criteria previously used to characterize prion strains. These included the induction of clinical signs in mice, the electrophoretic migration and glycoprofiles of CerPrPSc by Western blotting, PrPSc deposition by histoblot, cerebral vacuolization and PrPSc deposition by immunohistochemistry, and the denaturation characteristics of PrPSc. Based on these criteria, it appears that PMCA CWD prions retain the biological and biochemical properties of the originating CWD prions. However, we realize that each approach is limited in its ability to unequivocally define strain variation. For example, subtle differences in the pattern of PrPSc deposition in histoblots of individual mice may result from slight variances in the locations of coronal sections between mice, or from the times at which mice were sacrificed. The sensitive and specific paraffin-embedded tissue (PET) blot technique  may provide finer resolution for future comparative analyses. Furthermore, while the indistinguishable denaturation profiles and GdnHCl1/2 values of CerPrPSc in the brains of Tg mice dying from infection with naturally occurring or PMCA-derived CWD prions suggests comparable CerPrPSc structures, equivalent conformational stability does not necessarily indicate invariant conformations at all structural levels. Other approaches may reveal evidence of PrPSc structural differences. For example, infrared-spectroscopy distinguished the secondary structures of protease-resistant PrP from two hamster scrapie strains when immunobiochemical typing failed to detect differences . Fourier transform infrared-spectroscopy was also used to compare secondary structures of PMCA-generated and brain-derived protease-resistant PrP  as well as protease-resistant PrP products from seeded polymerization of recombinant PrP (rPrP-PMCA) .
Production of novel strains following abrogation of prion transmission barriers
Strain adaptation experiments, traditionally performed in vivo, often require years to generate prions with stable biological properties. While investigating the susceptibility of Tg mice to prions from other species provides a feasible approach to address the potential for inter-species prion transmission, the studies reported here demonstrate that abrogating the barrier to mouse RML prion transmission in Tg(CerPrP) mice required several hundred days followed by strain stabilization after serial passage. Our previous studies showed that Tg(CerPrP)1536+/− are also susceptible to sheep SSBP/1 scrapie prions, but with apparently less of a transmission barrier than mouse RML prions .
An important sequel to inter-species prion transmission is frequently the acquisition of new strain properties. The different patterns of CerPrPSc deposition in diseased Tg(CerPrP)1536+/− mice following RML infection indicates that abrogation of this transmission barrier most likely results in the formation and propagation of different prion isolates in individual mice. In this case we observed two general patterns by histoblotting and immunohistochemistry: diffuse CerPrPSc deposition in mice #4825 and #5297; and small plaque deposits in the case of mice #5302 and #5300. Materials from the histoblotted #4825 and #5302 mice were not available for serial transmission studies. At the time of writing, serial transmissions of prions from the brains of mice #5297 and #5300 are ongoing. The brain of mouse #4827 was used for serial transmission and full strain characterization in Tg(CerPrP)1536+/− mice. The substantial reduction and consistent time to onset of disease following serial passage of Cer/RML-4827 prions is characteristic of prion adaptation following transit across a species barrier. The ∼150 d mean incubation period of Cer/RML-4827 prions is ∼100 days shorter than either CWD or PMCA-generated CWD prions (Fig. 2), indicating adaptation of RML in Tg(CerPrP)1536+/− mice and the production of novel cervid prions with biological properties distinct from CWD. Additional detailed comparisons with Tg(CerPrP) mice infected with either naturally occurring or PMCA CWD prions were consistent with the notion that the biological properties of Cer/RML-4827 prions were distinct from CWD. In accordance with previous studies of experimentally-adapted hamster prion isolates in Tg mice expressing artificial chimeric PrP genes, which indicated that a change in the conformation of PrPSc accompanied the emergence of a new prion strain , the conformational stability of PrPSc changed following passage of mouse RML prions from wild type mice and subsequent adaptation to form Cer/RML-4827 prions in Tg(CerPrP)1536+/− mice (Fig. 6). Also in accordance with the process of prion adaptation, the profile of RML PrPSc glycosylation changed following transmission to Tg(CerPrP)1536+/− mice (Fig. 3), and there were distinct differences in the morphology and neuroanatomical distribution of PrPSc in Tg(CerPrP)1536+/− mice infected with Cer/RML-4827 and CWD prions.
We show that the adaptation of mouse RML prions, which required two passages in Tg(CerPrP) mice, can be accomplished by in vitro amplification of CerPrPC with heterologous RML prions to create cervid adapted RML prions in a matter of weeks. Accompanying this adaptation, the RML glycopattern changed from predominantly mono- to diglycosylated PrPSc, which is the form of CerPrPSc propagated in Tg(CerPrP) mice infected with RML, Cer/RML-4827 prions, and PMCA Cer/RML prions. Whereas 22 rounds were used to ensure the elimination of residual prion seed in the initial round of PMCA, it seems likely that PMCA-mediated inter-species transmissions can be accomplished with many fewer rounds of serial PMCA. Whether PMCA-mediated adaptation occurs with structural intermediates similar to the process in vivo is currently not known; however, since the properties of the initiating and resulting prions are, in most cases, likely to be distinct, it should be possible to determine the kinetics of prion adaption at each PMCA round. Should it be possible to reproducibly manipulate the extent of prion adaptation by varying the number of rounds of serial PMCA, then mechanistic studies of prion adaptation following inter-species transmission are likely to be considerably expedited by this approach.
Our studies convincingly show that PMCA of murine RML prions using Tg(CerPrP)1536+/− brain homogenate generates a novel strain of cervid-adapted prions with properties distinct from either naturally occurring or PMCA-generated CWD prions. It currently is less clear whether the PrPSc structures and strain properties of amplified and in vivo derived prions are equivalent. Direct comparisons of the strain properties of PMCA Cer/RML cervid prions and in vivo-adapted strains are complicated by our observations that in vivo adaptation gives rise to individual isolates with different strain-related properties, at least as judged by histoblot and immunohistochemical profiles of PrPSc. While certain strain-related attributes, including comparably rapid prion incubation times, and similar denaturation profiles of CerPrPSc after infection, suggest shared biological properties between Cer/RML-4827 and PMCA Cer/RML prions, other differences, including targeting of cerebral PrPSc deposition of Tg(CerPrP)1536+/− mice infected with Cer/RML-4827 and PMCA Cer/RML prions, point to divergent strain properties and thus would rather argue for different strains. Whether inter-species PMCA-mediated prion adaptation also results in the generation of multiple and distinct prion strains remains to be determined, but our limited analyses consisting of uniform histoblot profiles, reproducible onsets of disease, and similar conformation stabilities of CerPrPSc in individual infected mice may indicate that PMCA selectively and stably propagates distinct strains following abrogation of a species barrier.
Finally, we note that under certain conditions, PMCA may result in the spontaneous formation of PK-resistant PrP species  and de novo generated infectivity under conditions that do not involve seeding with infectious prions . While it would be of considerable interest to determine the biological properties of spontaneously-produced cervid prions by PMCA of CerPrPC, we feel that the possibility of spontaneous generation of infectivity in the context of the current studies is remote. The experiments of Deleault and co-workers using purified PrPC plus poly(A) RNA, indicated that spontaneous generation of PrPSc was a stochastic and relatively infrequent event, estimated at <1 conversion event per 6×1011 input PrPC molecules per PMCA round. Consequently, amplification of preexisting PrPSc molecules was considered an unlikely origin for PrPSc formation under these conditions. In contrast, in the studies reported here where PMCA reactions were seeded with either CWD or RML prions, high levels of protease-resistant PrP were amplified at each round of serial PMCA and remained consistently so during both intra- and inter-species PMCA-mediated prion amplification (Fig. 1A and B). In control experiments using unseeded healthy Tg(CerPrP)1536+/− brain extract, 10 passages of serial PMCA failed to generate protease-resistant PrP (data not shown). While we have also spontaneously generated PrPSc without the addition of prion seeds (Soto and co-workers, unpublished results; Castilla and co-workers, unpublished results), the PMCA conditions required to accomplish this required modification from the standard PMCA conditions used in the current and previous studies. Standard serial PMCA conditions in which healthy hamster brain homogenate was serially diluted into itself in the absence of prion seed failed to produce protease-resistant PrP following the same number of PMCA cycles which resulted in amplification of hamster 263K prions . In a larger experiment, samples of healthy brain homogenate from 10 different mice and hamsters were subjected to serial rounds of PMCA amplification in the absence of PrPSc seed using the PMCA conditions used in study. Following 20 rounds of serial PMCA, we did not observe de novo formation of PrPSc, nor did these materials, when inoculated into wild-type animals, induce disease after >400 d (Soto and co-workers, unpublished observations). For these reasons we feel that the generation of PrPSc and cervid prions reported in the present study, when normal brain homogenate from Tg(CerPrP)1536+/− mice was mixed with prion seeds, is unlikely to be influenced by spontaneous, de novo generated infectivity.
Materials and Methods
Preparation of tissue homogenates for PMCA
Healthy Tg(CerPrP)1536+/− mice were perfused with phosphate-buffered saline (PBS) plus 5 mM EDTA immediately prior to harvesting the tissue. Ten % brain homogenates (w/v) were prepared in conversion buffer which consisted of PBS containing NaCl 150 mM, 1.0% Triton X-100, and the complete™ cocktail of protease inhibitors (Roche, Mannheim, Germany). The samples were clarified by a brief, low-speed centrifugation (1500 rpm for 30 s) using an Eppendorf centrifuge (Hamburg, Germany).
Serial replication of prions in vitro by PMCA
A 1∶10 dilution of 10% brain homogenate from clinically sick 04-22412 infected mule deer or RML infected mice was diluted into a 10% brain homogenate from Tg(CerPrP)1536+/− mice. Samples in 0.2 ml PCR tubes were positioned on an adaptor placed on the plate holder of a microsonicator (Misonix Model 3000, Farmingdale, NY). Each PMCA cycle consisted of 30 min incubation at 37°C followed by a 20 s pulse of sonication set at potency of 7. Samples were incubated without shaking immersed in the water of the sonicator bath. After a round of 36 cycles, a 10 µl aliquot of the amplified material was diluted into 90 µl of additional Tg(CerPrP)1536+/− mouse brain homogenate and a new round of 36 PMCA cycles was performed. This procedure was repeated for 22 rounds. The detailed protocol for PMCA, including reagents, solutions and troubleshooting, has been published elsewhere , –.
Production and characterization of transgenic mice, and sources and preparation of inocula
Tg mice expressing deer PrP, referred to as Tg(CerPrP)1536 have been described previously . While we showed that CWD prion incubation times are more rapid in Tg(CerPrP)1536 homozygous for the transgene array than hemizygous Tg(CerPrP)1536 mice , because of difficulties associated with breeding homozygous Tg(CerPrP)1536 mice we have maintained this line in the hemizygous state by breeding with Prnp0/0 mice. Such mice are therefore referred to as Tg(CerPrP)1536+/− mice.
CWD prions were derived from a diseased female mule deer, referred to as 04-22412 UWVS ESW/JEJ, but abbreviated here as 04-22412. The animal was homozygous for the polymorphic codon 225, encoding serine at this location. The RML isolate was originally a kind gift from Byron Caughey (Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, Hamilton, MT) and was passaged by intracerebral inoculation of inbred FVB/N mice at the University of Kentucky.
Ten % (w/v) homogenates, in phosphate buffered saline (PBS) lacking calcium and magnesium ions, of cervid and mouse brains were prepared by repeated extrusion through an 18 gauge followed by a 21 gauge syringe needle.
Determination of Incubation Periods
Groups of anesthetized mice were inoculated intracerebrally with 30 µl of 1% (w/v) brain extracts prepared and diluted in PBS, or 1% v/v of the final PMCA product diluted in PBS. Inoculated mice were diagnosed with prion disease following the progressive development of at least three signs including truncal ataxia, ‘plastic’ tail, loss of extensor reflex, difficulty righting, and slowed movement. The time from inoculation to the onset of definitive and subsequently progressive clinical signs is referred to as the incubation time.
Analysis of PrP in CNS
For PrP analysis in brain extracts, total protein content from 10% brain homogenates prepared in PBS was determined by bicinchoninic acid (BCA) assay (Pierce Biotechnology Inc., Rockford, IL). Brain extracts were either untreated or treated with 40 µg/ml PK for one hour at 37°C in the presence of 2% sarkosyl. Protease digestion was terminated with 4 mM phenyl methyl sulfonyl fluoride (PMSF). Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins thus resolved were electrophoretically transferred to PVDF-FL membranes (Millipore, Billerica, MA). Membranes were probed with mAb 6H4 , or the Hum-P anti-PrP recombinant Fab  followed by horse radish peroxidase-conjugated sheep anti-mouse IgG or goat anti-human secondary antibody respectively. Signal was developed using ECL-plus detection (Amersham), and analyzed using a FLA-5000 scanner (Fuji).
Histoblots of 10 µm thick cryostat sections were generated and transferred to nitrocellulose as previously described . Histoblots were immunostained with the Hum-P anti-PrP recombinant Fab followed by alkaline phosphatase-conjugated goat anti-human secondary antibody. Images were captured using a Nikon SM21000 microscope with Photometrics Coolsnap CF digital imager and processed using MetaMorph software
The unfolding characteristics of PrPSc in brain homogenates of terminally sick mice were analyzed using a Western blot-based conformational stability assay ,, which is a modification of the original ELISA based protocol .
Analysis of PrP in the brains of Tg mice by immunohistochemistry was performed as previously described  using anti-PrP mAb 6H4  as primary antibody and IgG1 biotinylated goat anti-mouse secondary antibody (Southern Biotech). Digitized images for figures were obtained by light microscopy using a Nikon Eclipse E600 microscope equipped with a Nikon DMX 1200F digital camera.
We thank Dr. Anthony Williamson, Scripps Research Institute, La Jolla, and our colleagues at Prionics AG, Schlieren, Switzerland, for the kind gifts of Hum-P anti-PrP recombinant Fab, and mAb 6H4 respectively. We gratefully acknowledge the excellent technical assistance of Chu Chun Weng. We also thank Drs. Chongsuk Ryou and Alan Kaplan and members of the Telling lab for critical assessment of the manuscript and helpful suggestions.
Conceived and designed the experiments: KMG JC CS GCT. Performed the experiments: KMG JC TSS DLN. Analyzed the data: KMG JC CS GCT. Contributed reagents/materials/analysis tools: JEJ. Wrote the paper: KMG JC GCT.
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