Figures
Human herpesvirus-7 (HHV-7) U21 causes redistribution of NK-activating ligands to lysosomes.
Herpesviruses have evolved a stunning array of mechanisms to escape immune detection, both by CTLs and NK cells. The HHV-7 U21 open reading frame encodes a protein that can bind to and reroute both class I MHC molecules and the NK-activating ligand ULBP1 to the lysosomal compartment. This deconvolved widefield epifluorescence micrograph shows co-localization of the NK-activating ligand ULBP1 with lamp2, a lysosomal membrane protein, in U21-expressing astrocytoma cells. Alexafluor-488- and -598-conjugated secondary antibodies were used to label antibodies directed against lamp2 (green) and HHV-7 U21 (magenta). Light green-white indicates co-localization (see Schneider and Hudson, doi:10.1371/journal.ppat.1002362).
Image Credit: Amy W. Hudson and Christine L. Schneider, Department of Microbiology and Molecular Genetics, Medical College of Wisconsin
Citation: (2011) PLoS Pathogens Issue Image | Vol. 7(11) November 2011. PLoS Pathog 7(11): ev07.i11. https://doi.org/10.1371/image.ppat.v07.i11
Published: November 17, 2011
Copyright: © 2011 Hudson, Schneider. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Herpesviruses have evolved a stunning array of mechanisms to escape immune detection, both by CTLs and NK cells. The HHV-7 U21 open reading frame encodes a protein that can bind to and reroute both class I MHC molecules and the NK-activating ligand ULBP1 to the lysosomal compartment. This deconvolved widefield epifluorescence micrograph shows co-localization of the NK-activating ligand ULBP1 with lamp2, a lysosomal membrane protein, in U21-expressing astrocytoma cells. Alexafluor-488- and -598-conjugated secondary antibodies were used to label antibodies directed against lamp2 (green) and HHV-7 U21 (magenta). Light green-white indicates co-localization (see Schneider and Hudson, doi:10.1371/journal.ppat.1002362).
Image Credit: Amy W. Hudson and Christine L. Schneider, Department of Microbiology and Molecular Genetics, Medical College of Wisconsin