A-B, BJAB cells were transfected with pcDNA3.1-flag-LANA or pcDNA3.1-flag vector. 48 hours later, cells were harvested and fixed for immunofluorescence. C-D, BC-3 shCr and LANA knocked down BC-3 cells were harvested and fixed for immunofluorescence. Cells were stained with anti-Sgo1, centromere and LANA antibodies. The columns at right represent colocalization between Sgo1 and Centromere. E-F, To detect interaction between phosphorylated H2AT120 and Sgo1, phosphorylated H2AT120 or Sgo1 antibody were used for IP. western blot was done with indicated antibodies. G-L, BJAB transfected with empty vector plasmid, BJAB cells transfected with LANA, or LANA plus NNLS; BC-3 shCr, BC-3 shLANA and BC-3 shCr transfected with NNLS were immunostained with anti-centromere antibody (red) and LANA (green). DNA was stained with DAPI (blue). Average distances between paired anti-centromere antibody signals in each cell line are shown in the right panel. The mean scores were examined by using Student’s t-test. The p-value for G-H-I and J-K-L are as following. G-H: p = 5.18731E-06, G-I: p = 0.000881027, H-I: p = 0.021685667, J-K: p = 0.00012466, J-L: p = 0.016699138, K-L:p = 0.00456501.
- 1. Lang F, Sun Z, Pei Y, Singh RK, Jha HC, Robertson ES (2018) Shugoshin 1 is dislocated by KSHV-encoded LANA inducing aneuploidy. PLoS Pathog 14(9): e1007253. https://doi.org/10.1371/journal.ppat.1007253 pmid:30212568
Citation: Lang F, Sun Z, Pei Y, Singh RK, Jha HC, Robertson ES (2019) Correction: Shugoshin 1 is dislocated by KSHV-encoded LANA inducing aneuploidy. PLoS Pathog 15(4): e1007732. https://doi.org/10.1371/journal.ppat.1007732
Published: April 9, 2019
Copyright: © 2019 Lang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.