The interaction between schistosomiasis and Salmonella is a particularly important issue in Africa, where dual infection by the parasite and the bacterium are likely common. In this review, the ways in which schistosomiasis affects human biology as it relates to Salmonella are described. Those who are infected by both organisms experience reduced immunological functioning, exhibit irreversible organ damage due to prolonged schistosomiasis infection, and become latent carriers of Salmonella enterica serotypes Typhi and Paratyphi and S. Typhimurium. The sequestration of the bacteria in the parasite leads to ineffective antibiotic treatment because the bacteria cannot be completely killed, and lingering infection may then lead to antimicrobial resistance. These manifestations are likely not just for those dually infected but also for those first infected with schistosomes and, later, Salmonella. More data are needed to better understand dual infection, particularly as it may impact treatment and prevention of schistosomiasis and Salmonella in sub-Saharan Africa.
Citation: Hsiao A, Toy T, Seo HJ, Marks F (2016) Interaction between Salmonella and Schistosomiasis: A Review. PLoS Pathog 12(12): e1005928. https://doi.org/10.1371/journal.ppat.1005928
Editor: James B. Lok, University of Pennsylvania, UNITED STATES
Published: December 1, 2016
Copyright: © 2016 Hsiao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This manuscript was supported by core funding to IVI provided by the governments of the Republic of Korea and Sweden. The funders had no role in study design, data collection and analysis, decision to public, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Substantial progress has been made in reducing the incidence of communicable diseases globally. In Africa, where the burden of communicable diseases is particularly high, the mortality rate of children under five years of age has dropped from 173 to 95 per 1,000 live births from 1990 to 2012 . This reduction in deaths among children under five are a result of a combination of efforts, including increased access to clean water, sanitation, and improved hygiene, as well as increased rates of immunizations for vaccine-preventable diseases.
There are, however, still a number of preventable diseases that are neglected, including Salmonella and schistosomiasis. While each of these diseases has a significant impact on morbidity and mortality in sub-Saharan Africa, coinfection has important implications for country control and prevention plans. The following review summarizes the current state of knowledge on the biological interactions between Sal. enterica subtypes Typhi and Paratyphi and invasive non-typhoidal S. Typhimurium (iNTS) and schistosomiasis, as well as the burden of each in the context of sub-Saharan Africa.
Burden of Salmonella in Africa
Typhoid and paratyphoid fever are major causes of morbidity and mortality globally, causing an estimated 21.7 million illnesses and 217,000 deaths annually [2, 3]. Invasive nontyphoidal salmonellosis also contributes significantly to the global burden of disease, with an estimated 3.4 million cases and a case fatality rate of 20% . Yet, data specific to the African region are limited. Salmonella serotypes Typhi (S. Typhi) and Paratyphi A, B, and C, are the best described serotypes that cause typhoid fever (TF) and paratyphoid fever, respectively . Both serotypes of the bacteria act by invading the small intestine and entering the bloodstream and other organs (e.g., the liver and spleen) where they multiply further before re-entering the bloodstream. Clinical presentation varies from mild-grade fever to abdominal discomfort and complications including intestinal perforations . Practically, the diagnostic gold standard generally involves performing a blood culture to isolate the bacteria. Once confirmed, treatment often consists of antibiotics . Relapses can occur in 5%–10% of patients .
Despite a clear understanding of disease mechanisms and treatment, lack of diagnostic capabilities and surveillance systems, among other factors, has made it difficult to accurately describe the burden of TF (and paratyphoid fever) and iNTS disease in Africa. In 2008, the World Health Organization (WHO) expressed the need for more epidemiological data to estimate the incidence of Salmonella in Africa . Few studies have characterized the burden in this region. Mogasale et al. estimates incidence from 123 per 100,000 in West Africa, to 195 per 100,000 in South Africa, to as high as 465 per 100,000 persons in East Africa . A systematic review of 25 studies found an incidence of 725 per 100,000 in sub-Saharan Africa . Other country-specific estimates in Africa are similarly within these ranges [10, 11].
African countries will be better able to plan for Salmonella control as new data emerge, though other challenges will arise. Namely, there is increasing concern in the international community that infection of parasites, sometimes multiple parasites, may compromise the body’s ability to deploy a protective response to other acute bacterial or viral infections. Consequently, immunological responses to control and prevention strategies such as vaccinations may be attenuated in the presence of other infections commonly found in developing countries [12–14].
Burden of Schistosomiasis in Africa
Africa also suffers from the highest burden of neglected tropical diseases (NTDs), including schistosomiasis (also known as bilharzia), an infectious chronic disease caused by parasitic worms found in fresh water . There are five main species of the worm, but Schistosoma mansoni and S. haematobium are predominantly found in Africa and manifest in the form of intestinal and urogenital schistosomiasis, respectively . Schistosomiasis ranks second only to malaria amongst cases of parasite-associated mortality, killing an estimated 280,000 people annually in Africa alone . In 2013, more than 40 million people were treated for schistosomiasis, though at least 261 million people required preventive treatment, >90% of whom live in Africa . The few schistosomiasis seroprevalence studies that exist indicate that 90% of those living in high-risk areas will acquire an infection at some point in their lives, which often occurs before the age of ten. In low-risk areas, between 25% to 40% will be infected by the age of 35 [18, 19]. Fragile health systems coupled with lack of safe water, sanitation, and hygiene in many areas throughout Africa make schistosomiasis particularly challenging to control .
Clinical underdetection of schistosomiasis is common. The current gold standard diagnostic is microscopic examination of stool or urine for eggs , though it is not sensitive enough to detect mild infections where adult worms have not produced eggs in numbers sufficient for detection. Some studies have found that upwards of 20%–30% of active infections are missed in stool or urine samples, as eggs are not always passed into the specimen on the day of testing [22–24]. Thus, the reported prevalence of schistosomiasis is likely to be an underestimate. By some estimates, the burden of active cases of schistosomiasis in 2007 may have been between 391 to 587 million people worldwide .
Schistosomiasis can also cause long-term effects beyond the period of active infection. For example, infections have been found to be significantly associated with anemia, chronic pain, diarrhea, exercise intolerance, and malnutrition . The parasite causes acute granulomatous and fibrotic injury to bodily organs that can lead to chronic disability. Based on a meta-analysis of 135 randomized and observational studies of schistosomiasis, the parasitic infection is associated with a number of disability-linked morbidities. Repeated cycles of infection are common in endemic areas where infection prevention and control measures (including control of intermediate hosts) are lacking. If left untreated, the worms live within the host for an average of two to five years. Frequently, however, those at risk of multiple waves of infection will generally experience active infection for 15 or more years on average . Schistosomiasis can be treated with praziquantel, which kills the adult worms in the body . Treatment with praziquantel is generally safe, even in those living with decreased immune functioning, such as those with HIV . Thus, in high transmission areas, WHO recommends that treatment be repeated yearly for a number of years, and cases monitored to determine treatment effectiveness. The greatest limitation to schistosomiasis control, however, is availability of praziquantel . While efforts have been made to improve access, including the use of cheaper generics, WHO estimates that only 13.1% of people globally who required praziquantel treatment received it . Lai et al. estimate that 123 million doses of praziquantel are needed per year to treat school-aged children, and 247 million are needed for all affected populations . While access to treatment is important, control of schistosomiasis requires a multipronged strategy that involves interrupting the chain of transmission by ensuring that water is free of parasites and basic sanitation is adhered to, especially as drug resistance to praziquantel becomes more of an issue [30, 31].
Interaction between Salmonella and Schistosomiasis
There has been limited information to date of the interaction between Salmonella and schistosomiasis. However, evidence suggests that this interaction warrants more attention, especially as additional evidence emerges about the true burden of typhoid fever and iNTS disease in Africa . Based on available data, typhoid and schistosomiasis have similar geographic distributions, and the highest burden of both NTDs falls on children under 15 years of age [18, 19, 33]. While studies do exist that assess the immunological response to schistosomal and Salmonella coinfection, most have been conducted on small samples of patients in Egypt and Brazil in the late 1980s . Few studies to our knowledge have been conducted in Africa (see Table 1) [35–39].
Reduced immunological functioning
Both Salmonella and schistosomal infection can affect liver and kidney function, which can consequently affect the pharmacokinetics and pharmacodynamics of biological metabolites in the bloodstream [40–42]. Chronic infection of schistosome eggs trapped in the tissues (most commonly in the kidney and liver, though may affect other organs as well) leads to the secretion of proteolytic enzymes that provoke inflammatory and granulomatous reactions. Over time, these reactions inflame the epidermal tissue layer, producing fibrotic deposits that increase the risk of infection by any number of organisms not limited to Salmonella . What is unknown, however, is the in vivo nature of the interaction between Salmonella and schistosomiasis: Does damage to the liver and kidneys due to schistosomiasis aggravate and prolong Salmonella infection, or vice versa?
Previous research examined the extent to which liver infection may affect levels of circulating biochemical compounds in blood serum . In a case-control study of 55 patients (20 of whom were normal controls), El-Hawy et al. found that phagocytosis, a major mechanism within the body’s immune system for removing pathogens and micro-organisms, was affected depending on the patient’s condition. Patients with noncomplicated typhoid who were treated with chloramphenicol exhibited increased phagocytic behavior when compared to normal controls. After the chloramphenicol course, however, phagocytic function did not normalize, but instead was depressed below normal values, suggesting that the drug may affect phagocytosis. Conversely, patients with chronic salmonellosis and S. mansoni (treated with ampicillin and praziquantel, respectively), and patients with hepatosplenic schistosomiasis and active S. mansoni (treated with only praziquantel) had depressed levels of phagocytosis—an indication that schistosomiasis may have altered the body’s immune response. Chronic Salmonella infection was found to be more difficult to treat in the group with S. mansoni. Though the mechanism is not fully understood, this may be due to a combination of factors, including multiplication of the Salmonella organism hiding within the schistosome and/or altered immune responses that more easily allow the bacteria to multiply inside the reticuloendothelial system cells . More data are needed to better understand how differing antibiotic and antiparasitic treatment regimens affect coinfected patients.
There have also been few studies on coinfection of Salmonella and schistosomes that suggest that presence of both conditions further worsens renal function. One Brazilian study analyzed renal involvement of eight patients with hepatosplenic schistosomiasis and prolonged Salmonella infection compared to eight patients with schistosomal glomerulopathy (a reduction of the ability of the kidneys to flush out physiologic byproducts from the bloodstream). The authors found that prolonged Salmonella infection exacerbates pre-existing schistosomal glomerulopathy due to renal damage caused by the prolonged infection . At least one other study has corroborated this finding: patients with coinfection of both Salmonella and schistosomiasis had a greater risk of developing glomerulonephritis, primarily due to Salmonella antigens .
Schistosomiasis patients as latent carriers of Salmonella
Typhoid fever and iNTS disease have also been found to recur until schistosomiasis is treated, leading some researchers to hypothesize that Salmonella is able to persist in the body by attaching to the schistosome. Because of this purported relationship, the Salmonella are protected from antibacterial drugs [46–48].
In one of the earliest studies, Young et al. collected S. mansoni worms from patients with salmonellosis. The samples cultured from the tegument of the worms tested positive for S. Paratyphi A. The authors concluded that there exists a symbiotic relationship between Salmonella and schistosomes that make dual infection in humans particularly difficult to diagnose and treat .
Similar observations have also been made with other species of Schistosoma. Expanding on our understanding of this association, LoVerde et al. used some strains of Salmonella Typhimurium, a serotype that infects humans as well as some animals, that contained a mutated gene and thus did not express pili (the hair-like appendage found on the surface of bacteria) . Other strains did express pili. Hamsters were infected with various strains of S. mansoni, S. haematobium, and S. japonicum and then sacrificed to remove the adult schistosomes. A subset of the worms’ digestive openings was ligatured to test whether Salmonella was associated with the host’s gut and/or with the surface tegument of the worm. The experiment revealed that S. Typhimurium was associated with each of the Schistosoma tested with the presence of either the pili or the worm’s ligature, though a greater proportion of female worms tested positive for Salmonella than did male worms. The presence of both the pili and worm’s ligature increased the likelihood of adhesion between bacterium and parasite when compared to the presence of just one of the appendages. Nevertheless, the study concluded that the pili of the Salmonella bacterium, as well as the worm’s digestive tract opening, may play an important role in adhesion of Salmonella to the parasite.
This finding was supported by an in vitro experiment that demonstrated that pili expression of the S. Typhimurium bacterium is important for attaching to the surface tegument of S. mansoni. In samples without pili, Salmonella was found to only colonize the digestive tract, as opposed to colonizing both the surface tegument (specifically to the mannose-like receptors) and the digestive tract in samples with pili. The mannose-like receptors that bind to mannose, a sugar monomer, were suspected to be glycolipids on the surface of the worms that allow for the binding of the pili of Salmonella. This was further confirmed by treating S. Typhimurium with antipili antibodies prior to incubation with schistosomes. Because the pili were blocked by antibodies, no bacterial agglutination was observed on the surface of the schistosomes .
Tuazon et al. also studied the importance of pili in mice. Mice were infected initially with S. mansoni; a subset was then either challenged with piliated or nonpiliated S. Typhimurium. These mice were compared to another set that was administered praziquantel prior to the S. Typhimurium challenge. While mice challenged with nonpiliated bacterium compared to piliated had longer survival times, there was no significant difference in the mortality rates of the mice. However, the study demonstrated that Salmonella—regardless of pili presence—increased the mortality rate of mice infected with S. mansoni. Praziquantel administration prior to S. Typhimurium challenge prolonged survival significantly . More studies in humans are needed to better understand the relationship, as well as how the relationship may affect treatment options and detection of schistosomiasis and Salmonella.
The synergistic parasite–bacterium relationship in hamsters and mice has been shown in human hosts as well. A number of other studies in Egypt and in Africa have also characterized the relationship between schistosomal infections and urinary typhoid carriers. Because the schistosome eggs cause fibrotic lesions in the urinary tract, typhoid urinary tract infections are persistent and often lead to patients becoming chronic typhoid carriers [35, 53–56]. In one study, patients who were persistent urinary carriers of Salmonella did not initially respond to chloramphenicol treatment. Following treatment for bilharzia, however, a second course of the same antibiotic was administered and Salmonella was no longer found in urine cultures . Similarly, a study in Gabonese children found that relapse of Salmonella infection only occurred in children who were not first treated with niridazole or oltipraz for S. interaclatum (species that is localized in the colon and rectum). Whether antischistosomal treatment was administered appeared to affect the response to chloramphenicol or ampicillin treatment for Salmonella . It will also be important to understand whether protected Salmonella bacteria residing inside the schistosomes are shielded from the effects of antibacterial drugs, thereby avoiding the development of drug resistance.
Studies in Nigeria and Sudan have also found a strong, direct relationship between coinfection with Salmonella and either S. haematobium or S. mansoni [36–39]. One of the studies in Sudan found that the incidence of Salmonella was higher among children than adults with schistosomiasis, and that the incidence even increased if an individual (regardless of age) had schistosomiasis for more than a year . As a result of these findings, the authors of these four African studies conclude that patients with recurrent enteric fever living in schistosomiasis endemic regions should also be routinely screened for asymptomatic schistosomiasis. Modebe et al. went further to recommend that chemotherapy for Salmonella be administered prior to antischistosomal treatment, as the schistosome creates a place for the Salmonella bacterium to multiply before re-entering the bloodstream .
The present review suggests an interaction between Salmonella and schistosomiasis that will be important to explore further. In spite of the limited number of studies, infection with schistosomes may lead to irreversible damage and residual fibrotic deposits in organs that make one more susceptible to Salmonella infections. Further, parasitic infection reduces immunological functioning that makes it more difficult for the body to combat other harmful pathogens, including Salmonella. Proper treatment for schistosomes would be advisable prior to further treatment for Salmonella both to ensure the effectiveness of medications in eliminating the infection, as well as to prevent further drug resistance. Though, based on the limited evidence, it is not yet clear what the optimal time interval is between infection, treatment, and recovery from either pathogen. For example, a patient is receiving antibiotics for typhoid fever and is infected with schistosomiasis before the course of antibiotics has been completed. Is there a point at which the bacterial load is low enough that the two pathogens do not interact?
These findings have several implications for patients who are coinfected with typhoid and schistosomiasis. Firstly, if a schistosomiasis patient is diagnosed and treated for typhoid using antibiotics, treatment may not be complete—and may inadvertently lead to further resistance in strains of Salmonella. Treatment using first-line antibiotics for many S. Typhi strains has been decreasing in effectiveness, and many options are no longer available [57–60]. Secondly, incomplete treatment for Salmonella due to the schistosome’s shielding effect may give rise to a host of other bacteria control issues and health complications. As the study in Gabonese children discovered, the hidden bacteria residing in the schistosome may be shed, leading to a relapse of Salmonella. Consequently, a drug-resistant Salmonella strain may proliferate by spreading resistant genes to drug-sensitive bacteria over a prolonged period, resulting in an infection that is much more difficult to treat. Along similar lines, if an individual is coinfected, but unaware of coinfection, and received praziquantel as part of a periodic mass drug administration campaign, it is plausible that killing the schistosomes could lead to a systemic release of hidden Salmonella, giving rise to typhoid illness.
As part of its position paper in 2008, WHO recommended that typhoid fever vaccination programs be implemented in the context of other efforts to control the disease . These decisions should be made based on knowledge of the local epidemiology of typhoid—and based on the present review, should also include an understanding of the impact of the interactions between typhoid and other possible pathogens such as schistosomiasis. Despite the availability of licensed typhoid vaccines that offer protection against S. Typhi and some Paratyphi, one of which is prequalified by WHO, the number of countries that have incorporated typhoid vaccines into their vaccination programs remains low due to the short efficacy of the vaccines (three years)  and the fact that those polysaccharide vaccines cannot be administered to children over two years of age. There is currently no vaccine against the NTS subtypes. Based on the current body of knowledge, it is difficult to conclude whether the effectiveness of a typhoid vaccine would be diminished if adequate treatment and prevention of parasitic infections are not first provided . However, there have been studies that have shown evidence of the reduced protective immune response from vaccines (e.g., tuberculosis, tetanus), in the presence of schistosomiasis [62–64]. There is also no research to our knowledge on whether typhoid vaccination can reduce or stop shedding of Salmonella in existing typhoid patients—though typhoid vaccine manufacturers recommend against vaccinating chronic typhoid carriers and individuals with acute infection . Theoretically, vaccinating a typhoid carrier where the Salmonella bacterium’s pili is attached to the digestive tract of the worm would most likely not reduce shedding, as it is hidden from the antibodies produced. Consequently, if the vaccine is used in populations with a high burden of typhoid, the continual shedding of the bacterium in spite of vaccination may make the vaccine appear less effective. Understanding the incidence of coinfection between typhoid and schistosomiasis may help in better targeting the typhoid vaccine by prioritizing populations whose immune response would not be compromised by a concurrent schistosomiasis infection.
These key implications must be better understood and assessed. Further coordinated action is needed to improve typhoid surveillance, regulating and promoting appropriate use of antibiotics, and strengthening infection control and prevention plans. While typhoid conjugate vaccines are on the horizon that elicit longer-term protection and can be given to children over two years of age, the most promising vaccine in the pipeline is, at best, two years away from WHO prequalification and widespread use . If we are to have realistic plans for deploying future typhoid vaccines to high-risk populations, we must first understand the true burden of the co-occurrence of Salmonella and schistosomiasis and how the interaction between them impacts transmission dynamics.
By using existing surveillance infrastructures in sub-Saharan Africa, we have the opportunity now to study these coinfections in more detail . This is necessary for governments to prioritize interventions—such as vaccine programs and treatment options—that reduce morbidity and mortality. Particularly for children that suffer the highest burdens of both pathogens, breaking the cycle of chronic infection and reinfection with schistosomiasis in order to treat Salmonella ensures that children can be healthy enough to go to school and develop physically and mentally in this early stage of life. Schistosomiasis in children has been documented to lead to disabilities including anemia , which leads to stunted growth, and poorer short-term memory and mental abilities [68, 69]. Furthermore, because children with schistosomiasis are also likely to live in regions with other endemic helminths and infectious diseases (e.g., malaria), expanding surveillance of other endemic infections in the African region would provide additional opportunities to study the epidemiology of dual- and multipathogen infections. Indeed, some of the studies reviewed found that children and young adults were more likely to be Salmonella carriers than older adults [37, 56]. Though beyond the scope of the present review, the interaction between Salmonella and schistosomiasis reviewed herein suggests that parasitisms other than schistosomiasis (e.g., lymphatic filariasis, onchocerciasis, loa loa, and other soil-transmitted helminth infections) may have similarly unexplored interactions with Salmonella. Because treatment for schistosomiasis and other parasites often overlap, careful consideration should be given to the timing of all antihelmintic treatments.
By studying the combined impact of schistosomiasis and typhoid in Africa, policymakers will be better equipped to understand the nature of the interaction. While this is only one component of comprehensive schistosomiasis and typhoid prevention and control programs, additional data will help ministries of health better target interventions, such as improvements to water, sanitation, and hygiene to cut parasitic and bacterial transmission, praziquantel treatment programs, and targeted vaccinations (for both NTDs). Further, comprehensive strategies that consider the developmental impacts of chronic schistosomiasis will prevent needless prolonged suffering from salmonellosis.
- 1. World Health Organization, African Health Observatory. Atlas of African Health Statistics 2014. In: Africa ROf, editor. 2014.
- 2. Crump JA, Luby SP, Mintz ED. The global burden of typhoid fever. Bulletin of the World Health Organization. 2004;82(5):346–53. pmid:15298225
- 3. World Health Organization. Typhoid vaccines: WHO position paper. Releve epidemiologique hebdomadaire / Section d'hygiene du Secretariat de la Societe des Nations = Weekly epidemiological record / Health Section of the Secretariat of the League of Nations. 2008;83(6):49–59.
- 4. Trong TA, Nicholas AF, Melita AG, Karen HK, Frederick JA, John AC. Global Burden of Invasive Nontyphoidal Salmonella Disease, 2010. Emerging Infectious Disease journal. 2015;21(6):941.
- 5. World Health Organization. Salmonella (non-typhoidal), Fact sheet N°139 2013 [Available from: http://www.who.int/mediacentre/factsheets/fs139/en/.
- 6. World Health Organization, Department of Vaccines and BIologicals. Background document: The diagnosis, treatment and prevention of typhoid fever. 2003.
- 7. Parry CM, Hien TT, Dougan G, White NJ, Farrar JJ. Typhoid fever. (1533–4406 (Electronic)).
- 8. Mogasale V, Maskery B, Ochiai RL, Lee JS, Mogasale VV, Ramani E, et al. Burden of typhoid fever in low-income and middle-income countries: a systematic, literature-based update with risk-factor adjustment. The Lancet Global health. 2014;2(10):e570–80. pmid:25304633
- 9. Buckle GC, Walker CL, Black RE. Typhoid fever and paratyphoid fever: Systematic review to estimate global morbidity and mortality for 2010. (2047–2986 (Electronic)).
- 10. Breiman RF, Cosmas L, Njuguna H, Audi A, Olack B, Ochieng JB, et al. Population-Based Incidence of Typhoid Fever in an Urban Informal Settlement and a Rural Area in Kenya: Implications for Typhoid Vaccine Use in Africa. PloS one. 2012;7(1):e29119. pmid:22276105
- 11. Marks F, Adu-Sarkodie Y, Hunger F, Sarpong N, Ekuban S, Agyekum A, Nkrumah B, et al. Typhoid fever among children, Ghana. (1080–6059 (Electronic)).
- 12. King CH. Parasites and poverty: the case of schistosomiasis. Acta tropica. 2010;113(2):95–104. pmid:19962954
- 13. Labeaud AD, Malhotra I, King MJ, King CL, King CH. Do antenatal parasite infections devalue childhood vaccination? PLoS neglected tropical diseases. 2009;3(5):e442. pmid:19478847
- 14. Malhotra I, Mungai P, Wamachi A, Kioko J, Ouma JH, Kazura JW, et al. Helminth- and Bacillus Calmette-Guerin-induced immunity in children sensitized in utero to filariasis and schistosomiasis. Journal of immunology (Baltimore, Md: 1950). 1999;162(11):6843–8.
- 15. World Health Organization. 2013 Schistosomiasis: Status of schistosomiasis endemic countries [Available from: http://apps.who.int/neglected_diseases/ntddata/sch/sch.html.
- 16. World Health Organization. Schistosomiasis May 2015 [Available from: Available at http://www.who.int/mediacentre/factsheets/fs115/en/.
- 17. US Centers for Disease Control and Prevention. The Burden of Schistosomiasis (Schisto, Bilharzia, Snail Fever) 2011 [Available from: http://www.cdc.gov/globalhealth/ntd/diseases/schisto_burden.html.
- 18. Alarcon de Noya B, Ruiz R, Losada S, Colmenares C, Contreras R, Cesari IM, et al. Detection of schistosomiasis cases in low-transmission areas based on coprologic and serologic criteria The Venezuelan experience. Acta tropica. 2007;103(1):41–9. pmid:17606217
- 19. Stothard JR, Gabrielli AF. Schistosomiasis in African infants and preschool children: to treat or not to treat? Trends in parasitology. 2007;23(3):83–6. pmid:17241815
- 20. Xu J, Yu Q, Tchuente LA, Bergquist R, Sacko M, Utzinger J, et al. Enhancing collaboration between China and African countries for schistosomiasis control. (1474–4457 (Electronic)).
- 21. Gray DJ, Ross AG, Li YS, McManus DP. Diagnosis and management of schistosomiasis. BMJ (Clinical research ed). 2011;342:d2651.
- 22. Savioli L, Hatz C, Dixon H, Kisumku UM, Mott KE. Control of morbidity due to Schistosoma haematobium on Pemba Island: egg excretion and hematuria as indicators of infection. The American journal of tropical medicine and hygiene. 1990;43(3):289–95. pmid:2121056
- 23. De Vlas SJ, Engels D, Rabello AL, Oostburg BF, Van Lieshout L, Polderman AM, et al. Validation of a chart to estimate true Schistosoma mansoni prevalences from simple egg counts. Parasitology. 1997;114 (Pt 2):113–21.
- 24. Carabin H, Marshall CM, Joseph L, Riley S, Olveda R, McGarvey ST. Estimating the intensity of infection with Schistosoma japonicum in villagers of leyte, Philippines. Part I: a Bayesian cumulative logit model. The schistosomiasis transmission and ecology project (STEP). The American journal of tropical medicine and hygiene. 2005;72(6):745–53. pmid:15967758
- 25. King CH, Dickman K, Tisch DJ. Reassessment of the cost of chronic helmintic infection: a meta-analysis of disability-related outcomes in endemic schistosomiasis. Lancet (London, England). 2005;365(9470):1561–9.
- 26. King CH. Schistosomiasis: Challenges and Opportunities. Washington (DC): National Academies Press (US) Forum on Microbial Threats; 2011.
- 27. World Health Organization. Schistosomiasis: Strategy n.d. [Available from: http://www.who.int/schistosomiasis/strategy/en/.
- 28. Means AR, Burns P, Sinclair D, Walson JL. Antihelminthics in helminth-endemic areas: effects on HIV disease progression. (1469-493X (Electronic)).
- 29. Lai YS, Biedermann P, Ekpo UF, Garba A, Mathieu E, Midzi N, et al. Spatial distribution of schistosomiasis and treatment needs in sub-Saharan Africa: a systematic review and geostatistical analysis. (1474–4457 (Electronic)).
- 30. Savioli L, Fenwick A, Rollinson D, Albonico M, Ame SM. An achievable goal: control and elimination of schistosomiasis. (1474-547X (Electronic)).
- 31. Wang W, Wang L, Liang YS. Susceptibility or resistance of praziquantel in human schistosomiasis: a review. Parasitology research. 2012;111(5):1871–7. pmid:23052781
- 32. Marks F, von Kalckreuth V, Aaby P, Adu-Sarkodie Y, El Tayeb MA, Ali M, et al. The incidence of invasive Salmonella disease in sub-Saharan Africa: a multicenter population-based surveillance study. (Under Review).
- 33. Baker S, Hombach J, Marks F. What Have We Learned From the Typhoid Fever Surveillance in Africa Program? Clinical infectious diseases: an official publication of the Infectious Diseases Society of America. 2016;62 Suppl 1:S1–3.
- 34. el-Hawy AM, Anter A, Hegazi MM, Massoud A, Abou Tal S. Polymorphonuclear leucocyte phagocytosis in bilharzial and non bilharzial patients with enteric fevers. Journal of the Egyptian Society of Parasitology. 1985;15(1):343–52. pmid:3932530
- 35. Gendrel D, Richard-Lenoble D, Kombila M, Engohan E, Nardou M, Moussavou A, et al. Schistosoma intercalatum and relapses of Salmonella infection in children. The American journal of tropical medicine and hygiene. 1984;33(6):1166–9. pmid:6439063
- 36. Igwe NN E. INCIDENCEOFCO- INFECTION OF ENTERIC SALMONELLA WITH SCHISTOSOMA IN KACHIA LOCAL GOVERNMENT AREA OF KADUNA STATE, NIGERIA. Int J Trop Med Public Health. 2014;3(1):12–7.
- 37. Mohager M, Mohager S, Kaddam L. The Association between Schistosomiasis and Enteric Fever in a Single Schitosoma Endemic Area in Sudan. International Journal of Pharmaceutical Sciences and Research. 2014;5(6):2181–4.
- 38. Modebe AA, Nnachi AU, Ukaegbu CO, Tata N, Agah MV, Udu-Ibiam OE, et al. Dual infections of Enteric Salmonella species with Schistosoma mansoni among Patients from Two Hospitals in Jos, Nigeria. Journal of Applied & Environmental Microbiology. 2014;2(4):198–202.
- 39. Salem A, Bilal N, Ibrahim M. Frequent carriage of invasive Salmonellae amongst patients infected with schistosomiasis in Sudan. African Journal of Microbiology Research. 2015;9(8):543–8.
- 40. Lambertucci JR, Godoy P, Neves J, Bambirra EA, Ferreira MD. Glomerulonephritis in Salmonella-Schistosoma mansoni association. The American journal of tropical medicine and hygiene. 1988;38(1):97–102. pmid:2963552
- 41. Carvalho EM, Andrews BS, Martinelli R, Dutra M, Rocha H. Circulating immune complexes and rheumatoid factor in schistosomiasis and visceral leishmaniasis. The American journal of tropical medicine and hygiene. 1983;32(1):61–8. pmid:6824129
- 42. Higashi GI, Farid Z, Bassily S, Miner WF. Nephrotic syndrome in Schistosomiasis mansoni complicated by chronic salmonellosis. The American journal of tropical medicine and hygiene. 1975;24(4):713–4. pmid:239605
- 43. Gryseels B, Polman K, Clerinx J, Kestens L. Human schistosomiasis. Lancet (London, England). 2006;368(9541):1106–18.
- 44. Martinelli R, Pereira LJ, Brito E, Rocha H. Renal involvement in prolonged Salmonella bacteremia: the role of schistosomal glomerulopathy. Revista do Instituto de Medicina Tropical de Sao Paulo. 1992;34(3):193–8. pmid:1342069
- 45. Abdul-Fattah MM, Yossef SM, Ebraheem ME, Nasr ME, Hassan MA, Abdul Wahab SE. Schistosomal glomerulopathy: a putative role for commonly associated Salmonella infection. Journal of the Egyptian Society of Parasitology. 1995;25(1):165–73. pmid:7602159
- 46. Ross AG, Sleigh AC, Li Y, Davis GM, Williams GM, Jiang Z, et al. Schistosomiasis in the People's Republic of China: prospects and challenges for the 21st century. Clinical microbiology reviews. 2001;14(2):270–95. pmid:11292639
- 47. Muniz-Junqueira MI, Tosta CE, Prata A. [Schistosoma-associated chronic septicemic salmonellosis: evolution of knowledge and immunopathogenic mechanisms]. Revista da Sociedade Brasileira de Medicina Tropical. 2009;42(4):436–45. pmid:19802482
- 48. Barnhill AE, Novozhilova E, Day TA, Carlson SA. Schistosoma-associated Salmonella resist antibiotics via specific fimbrial attachments to the flatworm. Parasites & vectors. 2011;4:123.
- 49. Young SW, Higashi G, Kamel R, el-Abdin AZ, Mikhail IA. Interaction of salmonellae and schistosomes in host-parasite relations. Transactions of the Royal Society of Tropical Medicine and Hygiene. 1973;67(6):797–802. pmid:4596373
- 50. LoVerde PT, Amento C, Higashi GI. Parasite-parasite interaction of Salmonella typhimurium and Schistosoma. The Journal of infectious diseases. 1980;141(2):177–85. pmid:6988520
- 51. Melhem RF, LoVerde PT. Mechanism of interaction of Salmonella and Schistosoma species. Infection and immunity. 1984;44(2):274–81. pmid:6143728
- 52. Tuazon CU, Nash T, Cheever A, Neva F, Lininger L. Influence of salmonella bacteremia on the survival of mice infected with Schistosoma mansoni. The Journal of infectious diseases. 1985;151(6):1166–7. pmid:3998513
- 53. Ali OF, El Shabrawy M, Sabry FH, Fayad S. Relation of schistosomiasis to urinary typhoid carriers in Egypt. The Journal of the Egyptian Public Health Association. 1978;53(1–2):12–6. pmid:739205
- 54. Franco MM, Gelfand M. The relationship between bilharziasis and typhoid fever employing the Widal test as a screening procedure. The Central African journal of medicine. 1976;22(3):47–50. pmid:1268942
- 55. Halawani A, Abdalla A, Badran A. The relation between schistosomiasis and the urinary Salmonella carrier state. The American journal of tropical medicine and hygiene. 1960;9:371–3. pmid:14398939
- 56. Hathout Se-D. Relation of schistosomiasis to typhoid fever. The Journal of the Egyptian Public Health Association. 1970;45(1):145–56. pmid:4991891
- 57. Cooke FJ, Wain J. The emergence of antibiotic resistance in typhoid fever. Travel medicine and infectious disease.2(2):67–74. pmid:17291961
- 58. Hassing RJ, Goessens WH, Mevius DJ, van Pelt W, Mouton JW, Verbon A, et al. Decreased ciprofloxacin susceptibility in Salmonella Typhi and Paratyphi infections in ill-returned travellers: the impact on clinical outcome and future treatment options. European journal of clinical microbiology & infectious diseases: official publication of the European Society of Clinical Microbiology. 2013;32(10):1295–301.
- 59. Tatavarthy A, Luna VA, Amuso PT. How multidrug resistance in typhoid fever affects treatment options. Annals of the New York Academy of Sciences. 2014;1323:76–90. pmid:25069595
- 60. World Health Organization. Antibicrobial resistance April 2015 [Available from: http://www.who.int/mediacentre/factsheets/fs194/en/.
- 61. Sanofi Pasteur. Product Monograph: TYPHIM Vi. November, 2013.
- 62. Elias D, Akuffo H, Pawlowski A, Haile M, Schon T, Britton S. Schistosoma mansoni infection reduces the protective efficacy of BCG vaccination against virulent Mycobacterium tuberculosis. Vaccine. 2005;23(11):1326–34. pmid:15661380
- 63. Elias D, Wolday D, Akuffo H, Petros B, Bronner U, Britton S. Effect of deworming on human T cell responses to mycobacterial antigens in helminth-exposed individuals before and after bacille Calmette-Guerin (BCG) vaccination. Clinical and experimental immunology. 2001;123(2):219–25. pmid:11207651
- 64. Sabin EA, Araujo MI, Carvalho EM, Pearce EJ. Impairment of tetanus toxoid-specific Th1-like immune responses in humans infected with Schistosoma mansoni. The Journal of infectious diseases. 1996;173(1):269–72. pmid:8537675
- 65. Drugs.com. Typhoid Vaccine 2015 [Available from: http://www.drugs.com/monograph/typhoid-vaccine.html#r103.
- 66. Sahastrabuddhe S. Burden of Typhoid and Paratyphoid Fever and Strategies for Control. 14th World Congress on Public Health; February 13; Kolkata, India2015.
- 67. von Kalckreuth V, Konings F, Aaby P, Adu-Sarkodie Y, Ali M, Aseffa A, et al. The Typhoid Fever Surveillance in Africa Program (TSAP): Clinical, Diagnostic, and Epidemiological Methodologies. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America. 2016;62 Suppl 1:S9–s16.
- 68. Bell RM, Daly J, Kanengoni E, Jones JJ. The effects of endemic schistosomiasis and of hycanthone on the mental ability of African school children. (0035–9203 (Print)).
- 69. Jukes MC, Nokes CA, Alcock KJ, Lambo JK, Kihamia C, Ngorosho N, Mbise A, et al. Heavy schistosomiasis associated with poor short-term memory and slower reaction times in Tanzanian schoolchildren. (1360–2276 (Print)).