The Rvv two-component regulatory system regulates biofilm formation and colonization in Vibrio cholerae

The facultative human pathogen, Vibrio cholerae, employs two-component signal transduction systems (TCS) to sense and respond to environmental signals encountered during its infection cycle. TCSs consist of a sensor histidine kinase (HK) and a response regulator (RR); the V. cholerae genome encodes 43 HKs and 49 RRs, of which 25 are predicted to be cognate pairs. Using deletion mutants of each HK gene, we analyzed the transcription of vpsL, a biofilm gene required for Vibrio polysaccharide and biofilm formation. We found that a V. cholerae TCS that had not been studied before, now termed Rvv, controls biofilm gene transcription. The Rvv TCS is part of a three-gene operon that is present in 30% of Vibrionales species. The rvv operon encodes RvvA, the HK; RvvB, the cognate RR; and RvvC, a protein of unknown function. Deletion of rvvA increased transcription of biofilm genes and altered biofilm formation, while deletion of rvvB or rvvC lead to no changes in biofilm gene transcription. The phenotypes observed in ΔrvvA depend on RvvB. Mutating RvvB to mimic constitutively active and inactive versions of the RR only impacted phenotypes in the ΔrvvA genetic background. Mutating the conserved residue required for kinase activity in RvvA did not affect phenotypes, whereas mutation of the conserved residue required for phosphatase activity mimicked the phenotype of the rvvA mutant. Furthermore, ΔrvvA displayed a significant colonization defect which was dependent on RvvB and RvvB phosphorylation state, but not on VPS production. We found that RvvA’s phosphatase activity regulates biofilm gene transcription, biofilm formation, and colonization phenotypes. This is the first systematic analysis of the role of V. cholerae HKs in biofilm gene transcription and resulted in the identification of a new regulator of biofilm formation and virulence, advancing our understanding of the role TCSs play in regulating these critical cellular processes in V. cholerae.


Introduction
Two-component signal transduction systems (TCSs), composed typically of a sensor histidine kinase and a cognate response regulator, are one of the most prevalent regulatory mechanisms that allow microorganisms to detect, respond to, and adapt to changes in their extracellular or intracellular environments [1,2].In the most common type of two-component system, a cell membrane-associated sensor protein called a histidine kinase (HK) phosphorylates itself on a conserved histidine residue when it senses a change in the environment [3].A phosphoryl group is then transferred to a cytoplasmic response regulator (RR), and phosphorylation of the RR's conserved aspartate residue activates its output domain.While there are many kinds of RR output domains, most of them are DNA-binding domains that link phosphorylation to the transcriptional regulation of target genes.Many pathogenic microorganisms use TCSs to modulate gene transcription in response to changes in their environment and regulate diverse virulence-associated cellular processes [4].
In V. cholerae, TCSs also govern the formation and dispersal of biofilms [24].Biofilms enhance the pathogen's environmental survival, transmission, and infectivity to the human host.Biofilm formation is tightly regulated by diverse physical, chemical, and biological cues [25,26].V. cholerae biofilm formation requires production of the main matrix component, Vibrio polysaccharide (VPS) [27].The genes encoding the proteins for VPS production reside on two operons, vps-I, and vps-II [27].The vps operons are under the control of multiple regulators.Two orphan response regulators, VpsR and VpsT, activate the transcription of vps operons upon binding to the signaling molecule c-di-GMP [28][29][30][31].The quorum sensing (QS)
In earlier studies, we systematically evaluated the involvement of V. cholerae TCSs in intestinal colonization and biofilm formation by generating in-frame deletion mutants of each RR gene and studying the mutants for their in vivo colonization and biofilm gene transcription phenotypes [22,33].We wanted to determine how a similar study of HK genes would compare with this RR work.In-frame deletion strains for each of the 42 genes encoding HKs were generated.We performed phenotypic characterization of this deletion library by looking at how each V. cholerae HK affected the transcription of biofilm genes.We identified a previously uncharacterized HK, VCA0257, which we named RvvA (regulator of Vibrio vps), which regulates vps gene transcription and biofilm formation as well as in vivo colonization.We further show that the Rvv TCS operates by conventional phosphorylation-dependent signal transduction.

VCA0257/RvvA regulates vps gene transcription
There has been no systematic examination of the effect of HKs on V. cholerae biofilm gene transcription.To evaluate the role of the V. cholerae HKs in biofilm formation, we first generated in-frame deletion mutants for each of the 42 predicted HKs.We excluded VC1639 as we could not mutate this gene in the V. cholerae strain used here, A1552.Next, to identify the specific HKs that contribute to vps transcription, we measured the promoter activity of the vps-II operon genes under standard laboratory conditions as a proxy for biofilm gene transcription.We compared the transcriptional activity of the PvpsL-lux reporter in wild-type V. cholerae and the HK in-frame deletion strains.Most mutants did not exhibit any difference in vpsL transcription when compared to the wild type (Fig 1).Five, however, resulted in decreased vpsL transcription: VCA0565-(vxrA), VC1831-(cqsR), VCA0736-(luxQ), and VC1397-(cheA1).In contrast, deletion of nine increased vpsL transcription: VC1605, VC1156-7, VC0694, VC2748-(ntrB), VCA0522-(cqsS), VC1319-(carS), VC2136-(flrB), VCA0257, and VC2453-(varS).Only four of these have not been identified in previous work: VC1605, VC1156-7, VC0694, and VCA0257.In this study, we focused on VCA0257 (RvvA), because it was uncharacterized and its deletion resulted in the second highest increase in vpsL transcription (Fig 1).

rvv TCS regulates vps gene transcription
Analysis of the V. cholerae rvvA genomic context showed that VCA0257 (rvvA) is the first gene of a predicted three-gene operon composed of VCA0257, VCA0256 (rvvB), and VCA0255 (rvvC) (Fig 2A).RvvA is a HisKA family protein; it is 484 amino acids in length and it is predicted to have an N-terminal DUF3404 domain , a transmembrane region (249-271), and a C-terminal histidine kinase domain (272-484).The DUF3404 domain is functionally uncharacterized and is found associated with proteins containing Pfam domains PF02518 (HATPase_c) and PF00512 (HisKA).The RvvB protein is 223 amino acids in length with an N-terminal REC domain (10-121) and a C-terminal winged helix-turn-helix DNAbinding domain (126-220) (OmpR/PhoB-type-DNA-binding domain).The RvvC protein is 276 amino acids in length; it is predicted to be a periplasmic protein containing a DUF2861 domain .The DUF2861 domain is functionally uncharacterized and proteins containing this domain have no known function.
We next determined the role of the rvv loci in vps gene transcription.We found that vpsL promoter activity, measured using PvpsL-lux in the exponentially grown ΔrvvA strain, increased 5-fold compared to the wild type.This phenotype is complemented by introducing the wild-type copy of rvvA under the control of its native promoter in the Tn7 site (Fig 2B).These results demonstrate that RvvA negatively regulates vpsL gene transcription under the conditions tested.To determine whether the products of rvvB or rvvC are involved in this regulation, we generated in-frame deletion mutants of rvvB or rvvC and analyzed vpsL transcription in these strains.We observed that, in contrast to ΔrvvA, ΔrvvB and ΔrvvC strains showed no changes in vpsL transcription.We asked if the elevated vpsL transcription in ΔrvvA depended on RvvB or RvvC; we determined that while the ΔrvvAB strain has vpsL transcription similar to wild type, ΔrvvAC displayed increased vpsL transcription similar to ΔrvvA (Fig 2C).This finding suggests that, under the conditions tested, the increase in vpsL transcription seen in ΔrvvA requires RvvB but does not require RvvC.
We then compared biofilm formation between wild-type and ΔrvvA strains.Biofilms were grown under static conditions and quantified using BiofilmQ to calculate biofilm volume and roughness [40] (Fig 2D -2E).Overall, under the conditions tested, the total biomass was similar between wild type and ΔrvvA, but biofilm roughness in ΔrvvA was higher than that of the wild type.Our results show that RvvA alters the structural properties of biofilms, albeit slightly.We also compared the growth of ΔrvvA, a rvvA complemented strain, and the wild type (Fig 2F ).

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While the overall growth patterns of the strains were similar, we found that the generation time of the ΔrvvA (67.4min ± 1.7) was slightly increased compared to the wild type (63.6min ± 0.8).Biofilm formation and motility are inversely regulated, and cellular levels of the signaling molecule cyclic-di-GMP govern both processes.To see if Rvv influenced motility and cellular c-di-GMP levels, we measured swimming motility diameter and cellular c-di-GMP levels in rvv loci deletion strains using a c-di-GMP reporter.In contrast to vpsL promoter activity, the Δrvv strains showed no differences from the wild type in cellular c-di-GMP levels or motility (Fig 2G and 2H).Taken together, these results indicate that RvvA regulates vps gene transcription independently of c-di-GMP signaling.

RvvA acts upstream of the major biofilm regulators, VpsR and VpsT
V. cholerae core biofilm regulatory circuitry has two positive regulators, VpsR and VpsT, and a negative regulator, HapR.To gain further insight into the mechanism by which RvvA contributes to vpsL transcription, we investigated whether the absence of RvvA affects the transcription of vpsR and vpsT, which directly regulate vps gene transcription.We measured the promoter activity of the vpsR and vpsT genes using the transcriptional reporters PvpsR-lux and PvpsT-lux in wild-type, ΔrvvA, ΔrvvB, and ΔrvvAB strains.We observed that ΔrvvA has a 2-fold increase in vpsR and a 6-fold increase in vpsT transcription compared to the wild type (Fig 3A and 3B).In contrast, there was no statistically significant change in vpsR and vpsT transcription in either the ΔrvvB or ΔrvvAB strain.These findings suggest that RvvA regulates the transcription of vps structural and regulatory genes and that this regulation requires RvvB.
We conducted genetic interaction studies to determine how RvvA contributes to biofilm formation.To this end, we first generated ΔrvvAΔvpsR and ΔrvvAΔvpsT double mutants.We next measured PvpsL-lux promoter activity in ΔrvvAΔvpsR and ΔrvvAΔvpsT, ΔrvvA, ΔvpsR, and ΔvpsT strains (Fig 3C).In ΔvpsRΔrvvA, the vpsL transcription levels were similar to that of the ΔvpsR, suggesting RvvA requires VpsR for vpsL regulation.In ΔrvvAΔvpsT, vpsL ) ) ) Finally, we wanted to know if RvvA's effect on vpsL transcription depended on HapR, the main negative regulator of biofilm formation (Fig 3D).Deletion of hapR led to a 23-fold increase in vpsL transcription compared to the wild type.In contrast, a ΔhapRΔrvvA strain had a 51-fold increase in vpsL transcription compared to the wild type.The increase in vpsL transcription in ΔhapRΔrvvA strains was greater than in ΔhapR or ΔrvvA strains, indicating that HapR and RvvA modulate vpsL transcription via parallel regulatory pathways.

Mutations in the RvvA-HisKA and RvvB-REC domains impact vps gene transcription
The phosphorylation state of a RR determines its activity; we identified the aspartate residue that is predicted to be phosphorylated in the REC domain of RvvB using an amino acid sequence alignment of the V. cholerae OmpR family RRs (Figs 4A and S5A).We then substituted the aspartate residue in the REC domain of RvvB to mimic constitutively active (D57E) and inactive (D57A) versions and replaced the wild-type gene in the chromosome with the mutated versions of rvvB in both ΔrvvA and wild-type genetic backgrounds.We determined the impact of RvvB D57E and RvvB D57A substitutions on RvvAB regulation of vpsL (Fig 4C).In strains with RvvB D57E and RvvB D57A substitutions, vpsL transcription did not change compared to the wild type.In contrast, in the ΔrvvA strain, RvvB D57E increased vpsL transcription 5-fold, while in RvvB D57A , vpsL transcription phenocopied that of the wild type.This finding suggests that the increased transcription of vpsL in ΔrvvA requires an active RvvB.
RvvB D57E displayed increased vpsL transcription only in strains lacking RvvA, suggesting that an active Rvv TCS may upregulate vpsL but, under the conditions tested, RvvA keeps the system inactive.HKs mediate phosphorylation and subsequent dephosphorylation of their cognate RR [41,42]; we wondered if RvvA acts primarily as a phosphatase on RvvB under the conditions tested, preventing RvvB from modulating vpsL transcription.We thus aligned amino acid sequences of the HisKA Dhp domain of classic-type HKs in V. cholerae (Figs 4B and S5B) to determine the conserved histidine (H289) and glutamate (E290) of RvvA, predicted to be important for kinase activity, as well as the conserved threonine (T293), predicted to be important for phosphatase activity [41][42][43].We independently substituted each of these residues with alanine (H289A, E290A, T293A) and replaced the wild-type gene in the chromosome with the mutated versions of rvvA.In contrast to our prediction, we observed that in strains with rvvA substitutions predicted to impair kinase or phosphatase activity, PvpsL-lux activity was similar to the wild type (Fig 4D).

Mutations in the RvvA-HisKA and RvvB-REC domains impact rvv gene transcription
In the Rvv system, changes to biofilm gene transcription are evident only in the ΔrvvA genetic background.Therefore, we reasoned that the rvv operon's transcription is autoregulated by RvvB, and increased transcription of the rvv operon is needed for the observed phenotypes.To test this hypothesis, we generated a Prvv-lux transcriptional fusion and analyzed promoter activity in rvv single and double deletion strains (ΔrvvA, ΔrvvB, ΔrvvC, ΔrvvAB, ΔrvvAC, ΔrvvA::rvvA) (Fig 4E).Compared to the wild type, ΔrvvA, ΔrvvC, and ΔrvvAC showed 256-fold, 12-fold, and 248-fold increases in Prvv transcription during exponential growth, respectively.ΔrvvB strains resulted in very low Prvv transcription, either singly or in combination with rvvA.These findings suggest that the rvv operon is autoregulated and that RvvB is ) )

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required for this regulation.While the role of the predicted periplasmic protein RvvC in the modulation of RvvAB TCS signaling is unknown, the relatively small but reproducible rvv upregulation in ΔrvvC suggests that RvvC may be important for rvv activation.We next measured Prvv-lux activity in strains harboring the mutated versions of RvvA and RvvB.In strains with rvvA substitutions predicted to impair kinase activity (RvvA H289A and RvvA E290A ), Prvv-lux activity was similar to wild-type.In contrast, in strains with rvvA substitutions predicted to impair phosphatase function, RvvA T293A Prvv-lux activity was markedly increased, with a 130-fold increase in rvv transcription compared to the wild type (Fig 4E).
We additionally observed that in the ΔrvvArvvB D57E strain, Prvv-lux promoter activity increased 225-fold compared to the wild type; this transcriptional activation was abolished in the ΔrvvArvvB D57A strain.These findings suggest that, under the conditions tested, RvvA functions as a phosphatase keeping RvvB in a dephosphorylated state and resulting in basal rvv operon transcription.In contrast, in rvvA deletion and RvvA-phosphatase deficient strains, as well as in RvvB constitutively active strains, rvv operon transcription is increased, leading to activation of the Rvv TCS and associated phenotypes.

RvvA contributes to intestinal colonization
The involvement of V. cholerae TCSs in colonization and adaptability to host conditions is poorly understood.We evaluated the ability of RvvA to colonize the small intestine in an in vivo competition assay where the in vivo fitness of a mutant strain was compared to that of a wild-type strain using the infant mouse infection model.We found that ΔrvvA had a ~10-fold defect in in vivo colonization (Fig 5A).The wild-type copy of rvvA under the control of its native promoter in the Tn7 site (Fig 5A ) complements the colonization defect, further confirming that RvvA regulates in vivo colonization.We next tested colonization phenotypes of ΔrvvB and ΔrvvAΔrvvB; we observed that while ΔrvvB colonization is similar to that of the wildtype, ΔrvvAΔrvvB strain phenocopies the ΔrvvB strain.We next performed an in vivo competition assay between wild type and strains harboring different phoshomimetic versions of RvvB in the ΔrvvA genetic background (ΔrvvArvvB D57A and ΔrvvArvvB D57E ) (Fig 5B).The competitive index of ΔrvvArvvB D57A phenocopied wild type, while a ΔrvvArvvB D57E strain showed a ~10-fold defect in in vivo colonization.These results suggest that the colonization defect observed for ΔrvvA depends on the presence and phosphorylation state of RvvB.The absence of RvvA leads to increased vps transcription and a defect in in vivo colonization.To determine if the presence of vps contributes to the colonization defect seen in ΔrvvA, we performed in vivo competition assays with ΔrvvA, ΔvpsI-II, and ΔrvvA ΔvpsI-II and wild-type strains (Fig 5C).In this experiment, ΔrvvA and ΔrvvA ΔvpsI-II showed a ~6-fold defect compared to the wild type.We did not observe any difference in colonization between ΔrvvA and ΔrvvA ΔvpsI-II.In contrast, ΔrvvA and ΔrvvA ΔvpsI-II showed a statistically significant defect in colonization compared to WT or ΔvpsI-II.These results suggest that the RvvA colonization defect is independent of increased vps transcription.
Based on the above results, we hypothesized that ΔrvvA might downregulate the transcription of some virulence factors, leading to the observed defect in colonization.Therefore, we activity of the transcriptional fusion PvpsL-lux was measured from cells grown to exponential phase in the indicated strains.Individual data points (circles-RvvA present; triangles-RvvA deleted) of Relative Luminescent Units (RLU) are plotted with crossbars representing mean and standard deviation.Statistical significance was determined using a One-Way ANOVA and post-hoc Tukey's multiple comparisons test.Means from individual biological replicates (n � 6) were compared to that of wild type, and differences with an adjusted P value of � 0.01 were deemed significant.**, P � 0.001; ***, P � 0.0001.(E) Promoter activity of the transcriptional fusion Prvv-lux was measured from cells grown to exponential phase in the indicated strains.Individual data points (circles-RvvA present; triangles-RvvA deleted) of Relative Luminescent Units (RLU) are displayed on top of a boxplot for each strain.
https://doi.org/10.1371/journal.ppat.1011415.g004examined the transcription of core virulence factors in rvv mutants grown under virulenceinducing conditions.We analyzed ctx, (cholera toxin), and tcp, (toxin co-regulated pilus) promoter activity in wild-type, ΔrvvA, ΔrvvB, and ΔrvvAB strains using Pctx-lux and Ptcp-lux transcriptional fusions.(Fig 5D and 5E).Surprisingly, we found a small but consistent increase in ctx and tcp transcription in ΔrvvA.This observation suggests that changes in ctx and tcp transcription are not the source of ΔrvvA's defect during in vivo colonization.

Characterization of ΔrvvA's regulon
To better understand how the Rvv TCS contributes to V. cholerae infection, we compared the transcriptional profiles of wild type and ΔrvvA grown under virulence-inducing conditions using RNA-seq.Differentially expressed transcripts were defined as those having an adjusted p-value � 0.05, and a log 2 (fold-change) � ±1.This provided 55 upregulated and 19 downregulated transcripts that were differentially expressed in ΔrvvA compared to wild type under these conditions.
We next examined the Rvv regulon in the context of GO terms (biological process), where we used a cut-off of at least two differentially expressed genes being present for a given GO pathway.We found increased message abundance in ΔrvvA compared to wild type for genes predicted to be involved in regulation of DNA-templated transcription, carbohydrate metabolic process, overall metabolic process, proton transmembrane transport, cation transport, and protein transport.Conversely, we found decreased message abundance in ΔrvvA compared to wild type for genes predicted to be involved in transmembrane transport, proteolysis, phosphorelay signal transduction system, aromatic amino acid metabolic process, and signal transduction.
The transcripts that had the highest increase in abundance in ΔrvvA compared to wild type were those encoding genes located in the genomic region surrounding rvvA (VCA0257)

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(Fig 6A and 6C).The transcript abundance for VCA0258 and VCA0254, the upstream and downstream genes adjacent to the rvvABC operon, encoding genes of unknown function, were up-regulated 324-fold and 2044-fold, respectively.We now term these genes rvvE (VCA0258) and rvvD (VCA0254).We analyzed the promoter activity of PrvvA-lux, PrvvD-lux, and PrvvE-lux transcriptional fusions from cells grown in nutrient broth (Fig 6D).Consistent with our RNA-seq results, these transcriptional fusions also displayed increased promoter activity in ΔrvvA compared to wild type.The transcripts encoding the Rvv TCS system, rvvB (VCA0256), and rvvC (VCA0255) were also increased 178-fold, and 125-fold in ΔrvvA, respectively.
Of the V. cholerae virulence regulon, we found that only transcripts encoding toxR and toxS were differentially increased in ΔrvvA, further supporting our observation that ΔrvvA's in vivo fitness defect is not due to decreased transcription of core virulence factors.
Since the transcript abundance of genes surrounding the rvv loci was highest in ΔrvvA, we next analyzed the contribution of each rvv gene to the in vivo fitness defect.We deleted rvvB through rvvE in WT and ΔrvvA genetic backgrounds, and then performed in vivo competition assays (Fig 6E).In the WT background, deletion of rvvB, rvvC, rvvD, and rvvE did not impact in vivo fitness.In the ΔrvvA background, only deletion of rvvE abrogated the rvvA fitness defect by 4-fold.Taken together, these results suggest that increased transcription of rvv contributes to the in vivo fitness defect of ΔrvvA by a yet to be determined mechanism.

Comparative genomic analysis of the rvv loci
To further contextualize the role and possible function of the rvv locus, we performed a comprehensive reciprocal BLAST analysis of the structural and sequence conservation of rvvABC loci across Vibrionales representative genomes, superimposing the findings on a RecA phylogeny ( Fig 7).Our results show that the structure of the rvvABC locus is conserved in all the Vibrionales species in which homologs to any of its constituent genes are detected, suggesting that it operates as a functional operon across this clade.The rvvABC locus presents a markedly uneven distribution across the Vibrionales, and it is detected in only 55 (32%) of the 171 representative genome assemblies analyzed.The rvvABC operon is consistently detected in several large clades within the Photobacterium and Salinivibrio genera, as well as in different groups of marine Vibrio associated with mollusks echinoderms and other marine invertebrates (e.g.Vibrio neptunius, Vibrio echinoideorum, Vibrio atlanticus).Outside these clades, the rvvABC locus presents a scattered distribution that is strongly suggestive of lateral gene transfer.The genomic context of the rvvABC locus is highly variable (S4C Fig) , and shows evidence in several Vibrionales species (e.g.Vibrio tapetis) of association with chromosomally-encoded mobile genetic elements.The rvvABC locus is consistently detected in several close relatives of V. cholerae (Vibrio metoecus, Vibrio navarrensis, Vibrio vulnificus) suggesting that it was acquired by a recent ancestor of this group of human pathogens.The distribution of the rvvABC locus is in stark contrast with the one observed for the vxrABCDE operon, whose HK (vxrA) is the closest homolog of rvvA in the Vibrionales.The vxrABCDE operon structure and genomic context are highly conserved across the Vibrio genus (S2 and S4C Figs), suggesting an ancestral role for this operon in Vibrio biology [22].This suggests that the rvvABC locus implements a specialized signal transduction system that has been acquired or retained by different Vibrionales species.

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Competitive Index Vib rio fur nis sii GC F 00 63 64 Conservation of rvvABC in Vibrionales.The conservation of rvvABC genes across the Vibrionales order was assessed and structural similarity scores and individual gene percent identities were annotated on a RecA reference phylogeny using the iTOL web service [62].Amino acid % similarity of Rvv homologs is shown for each protein encoded in the rvv loci.% similarity is shown as a gradient from grey to blue, with blue representing the highest similarity.On the outermost ring, structural similarity of the rvv genomic region is visualized as a gradient from white to purple, with purple representing the highest structural similarity.https://doi.org/10.1371/journal.ppat.1011415.g007

Discussion
TCSs are commonly used by bacteria to sense and respond to their environment.Prototypical TCSs have a membrane-embedded histidine kinase that recognizes an external input and phosphorylates a response regulator that regulates gene transcription.In both the aquatic environment and the human host, V. cholerae encounters multiple changing inputs from the extracellular environment [44].TCSs allow the bacterium to integrate these signals and, in response, adjust various cellular processes.Biofilm formation is one critical cellular process contributing to the V. cholerae infection cycle; however, the repertoire of TCSs governing biofilm formation has not been fully evaluated.In this study, we systematically analyzed the impact of HKs on biofilm gene transcription in V. cholerae.We noticed that most HKs lacked either statistically significant deficiencies in biofilm gene transcription or had modest defects.These TCSs may have a role in biofilm formation; however, they are either not expressed, or their cognate signal is absent under the experimental conditions utilized in this study.
In our earlier work, we analyzed vpsL transcription in RR deletion mutants in V. cholerae under the same conditions used in our HK deletion screen [33].We found that 7 RR deletion mutants displayed significant changes in vpsL transcription.In ΔvpsR, ΔvpsT, ΔluxO, and ΔvxrB strains, vpsL transcription decreased compared to wild type.VpsR and VpsT are orphan response regulators.While VpsT activity does not depend on phosphorylation, VpsR activity is dependent upon the conserved aspartate residue predicted as a phosphorylation target [30,45,46].Cognate histidine kinase(s) of VpsR remain unknown.LuxO is phosphorylated by a phospho-relay involving LuxU, an Hpt, through histidine kinases LuxQ (VCA0736), VpsS (VC1445), CqsR (VC1831), and CqsS (VCA0522) [8,9,24].In this study, we found that vpsL transcription was decreased in ΔluxQ and ΔcqsR, increased in ΔcqsS, and unchanged in ΔvpsS.In ΔvxrA, a strain lacking the cognate histidine kinase of VxrB, vpsL transcription was decreased.In ΔcarR and ΔntrC strains, vpsL transcription increased compared to wild type [21,36].Consistent with this finding, we also observed that ΔcarS and ΔntrB displayed increased vpsL transcription.In ΔvarS, vpsL transcription was markedly increased, which is consistent with the known role of the VarSA system in regulating vps transcription and biofilm formation [6].Additionally, we observed that deletion of four previously uncharacterized HKs VC1156-7, VC0694, VC1605, and VCA0257 (RvvA) resulted in increased vpsL transcription.
We mainly focused on RvvABC, a novel regulator of vps and biofilm formation and pathogenicity.We showed that the sensor histidine kinase RvvA represses biofilm gene transcription and promotes mouse colonization.Somewhat surprisingly, given the large increase in vpsL, biofilms formed in ΔrvvA had modestly altered structural properties compared to the wild type.This finding correlated well with the observation that there were no changes in c-di-GMP levels or motility in ΔrvvA.This outcome suggests that RvvA is not a global c-di-GMP regulator, but instead has a more focused effect on vpsL transcription.RvvA repressed transcription of VpsR and VpsT, and epistasis analysis found that the increase in vpsL transcription seen in ΔrvvA was dependent on VpsR and VpsT, but not HapR.The increases in biofilm gene transcription in ΔrvvA were not the source of ΔrvvA's survival defect during in vivo colonization, as ΔrvvA ΔvpsI-II displayed the same competitive fitness as ΔrvvA.ctxA and tcpA transcription was increased in ΔrvvA compared to wild type, suggesting a change in virulence gene transcription was also not the cause of the in vivo fitness defect.Comparison of transcriptional profiles of wild type and ΔrvvA under virulence-inducing conditions supported the idea that differential transcription of core virulence factors is not driving the in vivo fitness defect of ΔrvvA.The rvv regulon comprises many genes of unknown function.Further investigation is needed to determine the mechanism by which the Rvv regulon modulates different functions in V. cholerae.
The phenotypes in ΔrvvA depend on the presence of RvvB, the predicted cognate response regulator of RvvA.In canonical two-component systems, the sensor histidine kinase activates the response regulator upon detecting an input signal, and the response regulator elicits cellular output.HKs are often bifunctional and can dephosphorylate their cognate RRs, and signals can stimulate either the kinase or phosphatase activity of the HK [41,42].We reason that, under the experimental conditions utilized in this study, RvvA's HK activating signal is not present, and therefore RvvA functions as a phosphatase.RvvA's input signal may modulate RvvA's kinase/phosphatase activity, shifting RvvA from predominantly phosphatase to predominantly kinase when a signal is present and coordinate biofilm formation through activation of RvvB (Fig 8).Two-component system genes must be transcribed at a basal level to produce enough sensor HK and regulators to detect and respond to specific signals.Numerous two-component systems contain both a constitutive and an autoregulated promoter, which is necessary to produce high levels of regulators to transduce and respond to signals, thereby allowing environmental adaptation [47].We determined that the rvv TCS is autoregulated.This feedback regulation would be energetically beneficial, particularly for facultative pathogens such as V. cholerae, because this strategy would prevent unnecessary activation of cellular processes.In support of this hypothesis, the mutation of a conserved residue implicated in phosphatase activity (RvvA T293A ), elevated rvv transcription similarly to the increase in transcription observed in ΔrvvA.
RvvB may be phosphorylated and active in ΔrvvA.In this case, a small-molecule donor, such as acetyl-phosphate, may phosphorylate RvvB in RvvA's absence, as seen for other RRs [48,49] (Fig 8).Alternatively, cross-talk may occur between RvvB and the HK of a different TCS [50] ( Fig 8).Phosphorylation of RvvB by another HK may not be detected if RvvA simultaneously dephosphorylates it.Only in the absence of RvvA could a phosphorylated version of RvvB persist in the cell and elicit a response.Further study is needed to determine the mechanisms that control Rvv TCS activation.
The RvvABC structural and sequence conservation analysis revealed that the RvvABC system shares similar domains with another V. cholerae TCS, VxrABC [22].The RvvA domain organization is similar to that the VxrABC HK, VxrA, with both possessing a periplasmic sensing region (SD) and a cytoplasmic DUF3404 domain (Pfam PF11884).The VxrA periplasmic domain has been crystallized, and revealed that it has a unique structural fold that forms an uncommon hairpin-swapped dimer.VxrA lacks a cytoplasmic linker region between the second transmembrane helix and the dimerization and histidine phosphotransfer (DHp) domain [51].Structural studies revealed that the conformational change brought about by the relative rotation of the two monomers in a VxrA-SD dimer might alter the connection of transmembrane helices and, consequently, the pairing of cytoplasmic DHp domains, thereby transferring the ligand-binding signal from the periplasmic SD to the cytoplasmic kinase domain [51].It is possible that VxrA and RvvA share similar activation mechanisms as many of the residues important for function in VxrA's sensing domain are conserved in RvvA.RRs of both the Rvv and Vxr systems, RvvB and VxrB, share an N-terminal REC domain and a Cterminal winged helix-turn-helix DNA-binding domain with RR of the OmpR family.Finally, RvvC and VxrC share ~20% sequence identity, are both predicted to be periplasmic, and contain a DUF2861 domain whose function remains unknown.
RvvA/VxrA and Rvvc/VxrC represent the first examples in which the phenotypic consequences of mutating proteins with DUF3404 or DUF2861 domains are explored.In previously published work, VxrA and VxrB positively control vpsL transcription and biofilm development.However, VxrC acts as a biofilm repressor, and this phenotype depends on the presence of VxrB.VxrC interaction with the periplasmic domain of VxrA appears to inhibit VxrA and VxRB activation, though the molecular details are unknown.In the Rvv system, under the conditions used in this study, RvvA and RvvC, albeit slightly, negatively control vpsL transcription; this phenotype depends on the presence of RvvB.Thus, in both systems, the predicted periplasmic component plays a role in signal transduction.The cognate signal(s) governing the activity of RvvA and VxrA systems is unknown and, similarly, the molecular details of the signal transduction mechanism and the role the periplasmic proteins RvvC and VxrC play in this process are yet to be determined.
Since the Rvv and Vxr systems share overall operon structure and domain organization, we undertook comparative genomics analysis to determine the phylogenetic distribution of these systems.Comparative genomics analysis of the rvvABC locus revealed that this locus is present in multiple Vibrionales species and maintains a conserved operon structure but presents an overall scattered distribution across this phylogenetic order.Additionally, when the rvvABC locus is found, there is no evidence of genomic context conservation.The phylogenetic heterogeneity of the rvvABC operon and its lack of genomic context conservation contrast with those observed in the vxrABCDE operon.The vxrA-encoded histidine kinase is highly conserved and displays substantial genomic context conservation across the Vibrio genus, indicative of an ancestral role in the Vibrio genome.The phylogenetic and genomic context evidence, therefore, makes a strong case for multiple instances of lateral gene transfer of the rvvABC locus within the Vibrionales order.This suggests that the rvvABC operon codes for a specialized signal transduction pathway that has been acquired or selectively kept by a diverse group of Vibrionales species with very different habitats, including the group of human pathogens exemplified by V. cholerae.Our findings indicate that the Rvv TCS is an important regulator of biofilm gene transcription and in vivo colonization in V. cholerae.Further work characterizing the purpose of this TCS in V. cholerae's life cycle may shed light on novel regulatory mechanisms connecting biofilm formation and virulence.

Bacterial strains and growth conditions
The strains and plasmids used in this study are listed in S1 Table .E. coli CC118-λpir and DH5α-λpir strains were used for DNA manipulation, and E. coli S17-1λpir strains were used for conjugation with V. cholerae.V. cholerae and E. coli strains were grown aerobically at 30˚C and 37˚C, respectively, unless stated otherwise.Luria-Bertani Miller (LB) broth contained 1% tryptone, 0.5% yeast extract, 1% NaCl [pH 7.5].LB agar medium included granulated agar (BD Difco, Franklin Lakes, NJ) at 1.5% (wt/vol); except for motility agar which was 0.3% (wt/vol).When AKI medium was used, media components and growth conditions were followed as previously described [52].Antibiotics and inducers were used, when necessary, at the following concentrations: ampicillin (Ap), 100 μg/mL; rifampicin (Rif), 100 μg/mL; gentamicin (Gm), 15 μg/mL; chloramphenicol (Cm), 20 μg/mL for E. coli and 5 μg/mL or 2.5 μg/mL for V. cholerae.Unless specified otherwise, overnight cultures for experiments were prepared as follows: strains were struck from frozen glycerol stock onto an LB-agar plate and grown at 30˚C overnight, 5 colonies were then inoculated into 5 mL LB media and grown overnight at 30˚C with aeration (200 rpm).

Strain and plasmid construction
Plasmids were constructed using standard molecular cloning techniques or the Gibson Assembly recombinant DNA technique (New England BioLabs, Ipswich, MA).In-frame gene deletions were generated through allelic exchange of native open reading frame (ORF) with the truncated ORF, as previously described [53].

Luminescence assay
V. cholerae strains harboring transcriptional reporters were grown overnight with aeration in LB broth supplemented with chloramphenicol 5 μg/mL.Cultures were diluted 1:200 into fresh LB containing chloramphenicol 2.5 μg/mL.The freshly inoculated cultures were grown aerobically at 30˚C to exponential phase (OD 600 of 0.3 to 0.4) and then luminescence was measured using a PerkinElmer Victor3 multilabel counter (PerkinElmer, Waltham, MA).For the HK single deletion PvpsL-lux screen, cells were diluted 1:200 from 5ml ON cultures into 200μl LB and grown in 96-well plates statically, but all conditions kept identical otherwise.For non-Pvps reporters, to identify how gene transcription changes over time, cells were grown in a 96-well plate in a heated plate reader for at least 15 hours, with OD 600 and luminescence measurements taken every 30 minutes.A representative measurement with exponential phase OD 600 was used for visualization and comparison.Luminescence activity is reported as relative luminescence units (RLU; counts min−1 ml−1/OD 600 unit).Assays were repeated for a minimum of three independent biological replicates, with three technical replicates measured for all assays.

c-di-GMP dual fluorescent reporter assay
Intracellular c-di-GMP levels were evaluated using a fluorescent reporter as previously described [54,55].V. cholerae strains harboring a c-di-GMP dual-fluorescent biosensor (pFY_4535) were grown overnight with aeration in LB broth supplemented with Gentamicin.Cultures were diluted 1:200 into fresh LB.The freshly inoculated cultures were grown aerobically at 30˚C to exponential phase (OD 600 of 0.3 to 0.4) and then fluorescence was measured in Corning 96-well, clear-bottom, black, polystyrene microplates using a PerkinElmer Victor3 multilabel counter (PerkinElmer, Waltham, MA).460/480 nm and 550/580 nm excitation/ emission filters were used to measure fluorescence intensity for Amcyan (normalizer) and TurboRFP, respectively.c-di-GMP levels are reported as relative fluorescence intensity (RFI).RFI was calculated from the ratio of fluorescence intensity of TurboRFP to Amcyan.Assays were repeated for a minimum of three independent biological replicates, with three technical replicates measured for all assays.

Swimming motility assay
Motility assays were performed in LB media containing 0.3% (wt/vol) agar.100 ml of LB agar media was dried at room temperature in 150mm diameter petri dishes.After 22h drying, single colonies of V. cholerae strains were stabbed into the motility plate.Strains were positioned with equidistant spacing from center and edges of the petri dish, as well as equal spacing from other strains.After 16h, the diameter of the swimming area surrounding the inoculum position was measured and compared.Assays were repeated for a minimum of four independent biological replicates; each motility plate was treated as an independent biological replicate.

Static biofilms and CLSM
Diluted overnight cultures of gfp-tagged V. cholerae strains were used for inoculation of static biofilms.1 milliliter of 1:200 diluted cells (OD 600 of 0.02) was introduced to a static biofilm chamber (Ibidi #80281).Post-inoculation, static biofilm chambers were incubated at 30˚C in LB with no aeration or movement.After 6-hours, static biofilms were gently washed twice in PBS.Biofilms were kept in PBS throughout imaging, and images were taken promptly after washing.Images of biofilms were captured with an LSM 880 (Zeiss, Jena, Germany), using an excitation wavelength of 488 nm and an emission wavelength of 543 nm.Three-dimensional (3D) images of the biofilms were processed using Imaris software (Bitplane, Zurich, Switzerland).All image processing parameters in Imaris (opacity, min, max, etc.) were kept identical between images for accurate comparison.BiofilmQ was used for quantitative analysis of biofilm parameters [40].

Growth curves
V. cholerae strains were grown aerobically overnight in LB.Strains were diluted 1:200 into one well of a 96-well plate in 200uL.96-well plates were grown in a plate reader at 30˚C.OD 600 measurements were taken hourly after brief shaking to stir the cells for the measurement.At least 3 biological replicates (independent overnight cultures) and 3 technical replicates (same overnight culture in different wells) were used for comparison of growth between different strains.

Intestinal colonization assay
In vivo competition assays for intestinal colonization determination were performed as described previously [56].In brief, cultures of relevant strains were grown to stationary phase PLOS PATHOGENS at 30˚C with aeration in LB broth.Each lacZ + V. cholerae mutant strain was mixed with the wild-type reference strain (lacZ -, otherwise wild-type) in a 1:1 ratio in 1x PBS.The inoculum was plated on LB agar plates containing 5-bromo-4-chloro-3-indoyl-β-D-galactopyranoside (X-gal) to differentiate colonies of the reference strain from mutants and determine input ratios.10 6 -10 7 cfu of the input inoculum mixture was oral gavage administered to groups of 5-7 anesthetized 5-day old CD-1 mice (Charles River Laboratories, Hollister, CA).At 22h post-inoculation, small intestines were removed, weighed, homogenized, and plated on appropriate selective and differential media to obtain output ratios.In vivo competitive indices were then calculated by dividing the small intestine output ratio by inoculum input ratio of mutant to wild-type strains.

RNA isolation
V. cholerae strains were grown overnight in LB broth at 37˚C, and then diluted 1:100 into 10 ml of fresh AKI medium in borosilicate glass test tubes (diameter: 15mm, height: 150mm), and grown statically at 37˚C.After 4 hours, cultures were transferred to 125ml flasks and grown on an orbital shaker with increased aeration (250 rpm).After 1 hour of increased aeration, 2 ml of each culture was centrifuged (16 K, 30 seconds), and the resulting pellet was immediately resuspended in 1 ml TRIzol (Invitrogen), flash-frozen, and subsequently stored at -80˚C until RNA isolation.Total RNA isolation was performed according to the TRIzol manufacturer's instructions.DNAse treatment, rRNA depletion (RiboZero Plus, Illumina), and library prep (Stranded RNA library preparation, Illumina) were performed according to manufacturer's instructions.Sample QC was performed via Bioanalyzer.Illumina sequencing was performed for paired-end 150 bp reads.

RNA-sequencing data analysis
Quality checks were performed on read data with FASTQC, version 0.11.9 [57].Trimming was not performed as it was deemed unneeded from FASTQC analysis output.Transcript abundance was quantified with Salmon, version 1.5.2, using a recently inferred V. cholerae transcriptome derived from the N16961 reference genome as an index [58,59].The resulting salmon quantification files were then imported into R via tximport [60].Counts were normalized and differential expression analysis was performed using DESeq2, version 1.32.0 [61].

Operon conservation analysis
The conservation of rvvABC genes was assessed using the operon_conserve_detect Python script (https://github.com/ErillLab/oprn_consv_calc).The script automates independent tBLASTN searches with each of the provided proteins for a given genomic locus against the NCBI RefSeq representative genomes database (ref_prok_rep_genomes) restricted to a taxon of interest (Vibrio (txid:662) and Vibrionales (txid: 135623)).tBLASTN hits were restricted to a maximum e-value of 10 −10 and a minimum coverage of 30%.Inferred homology was strengthened by filtering hits based on whether a BLASTP of the hit against the reference genome returned the reference locus protein (i.e., reverse BLAST).The nucleotide records for the resulting hits were grouped by genome using their assembly accession number.For each nucleotide record, hits to the reference locus are grouped into operons based on the following criteria: (1) all genes in an operon must be on the same strand, (2) no more than three features can span the distance between two hits to the reference operon, and (3) the intergenic distance between operon genes must be less than 150 base pairs.Locus structure is evaluated by computing the split distance of the locus, using its reference instance for normalization (see S1 Data).Given a reference locus organization with N genes, the set of possible gene-pairs within PLOS PATHOGENS the locus, P ref , has a size of N-1.For any given genome, the total possible number of each operon gene-pairs (M) is computed based on the number of hits to each reference gene in the specific gene-pair.The number of observed pairs T (pairs within predicted operons following the criteria above) is then computed, and for each pair the fraction T/M is considered the relative number of occurrences for that pair (R).The sum of relative occurrences, R, divided by the number of reference gene-pairs, P ref , is the structural similarity score, Y, ranging from 1 (total conservation) to zero (no pairs conserved).Structural similarity scores and individual gene percent identities were annotated on a reference phylogeny using the iTOL web service [62].The reference phylogeny was generated using Escherichia coli RecA (WP_000963143.1)homologous sequences detected by BLASTP in all species of interest using the phylo_seq_gen Python script (https://github.com/ErillLab/phylo_seq_gen).The RecA sequences were aligned with CLUSTALW, and the resulting alignment was filtered with Gblocks [63].This alignment was used to construct a Maximum Likelihood tree and infer bootstrap support (1,000 pseudoreplicates) using the MEGAX software suite [64].

1 0 9 5 PvpsL (RLU, 10 6 )Fig 1 .
Fig 1. Identification of HKs impacting vpsL transcription in Vibrio cholerae.Promoter activity of the transcriptional fusion PvpsL-lux was measured from cells grown to exponential phase in strains harboring deletions of individual HKs and WT.The orange coloring indicates the HK (VCA0257) that is the focus of this study.Individual data points of Relative Luminescent Units (RLU) are plotted with bars at the mean and error bars representing standard deviation.Statistical significance was determined using a One-Way ANOVA and post-hoc Tukey's multiple comparisons test.Means from individual biological replicates (n � 3) were compared to that of wild type, and differences with an adjusted P value of � 0.01 were deemed significant.***, P � 0.0001.https://doi.org/10.1371/journal.ppat.1011415.g001

Fig 4 .
Fig 4. Impact of RvvAB phosphotransfer mutations on Rvv phenotypes.(A) Seqlogo generated from amino acid sequence alignment of the REC domain of OmpR-like response regulators (RRs) in V. cholerae using ClustalO.The most conserved aspartate that was chosen for mutation is labeled (D57).(B) Seqlogo from amino acid sequence alignment of the HisKA DHp domain of Classic-type histidine kinases (HKs) in V. cholerae using ClustalO.Labels indicate the mutations generated in residues expected to impact kinase (H289) and phosphatase (T293) activity, respectively.(C-D) Promoter

Fig 5 .
Fig 5.The role of the rvv system in host colonization.(A,B, C).The competitive index (CI) of indicated mutant strains was analyzed and compared to the CI of wild type during colonization of the infant mouse intestine.Each data point represents the CI in an individual mouse.(A, B) Statistical significance was determined using a One-Way ANOVA and post-hoc Tukey's multiple comparisons test.Means from individual biological replicates (n � 6) were compared to that of wild type, and differences with an adjusted P value of � 0.01 were deemed significant.***, P � 0.0001.(C) Means from at least six biological replicates were compared by an unpaired ttest, and mean differences with a P value of � 0.01 were considered significant.ns, not significant.(D, E) Promoter activity of the transcriptional fusions PctxA-lux (D), and PtcpA-lux (E) were measured from cells grown to exponential phase in the indicated strains.Individual data points (circles-RvvA present; triangles-RvvA deleted) of Relative Luminescent Units (RLU) are displayed on top of a boxplot for each strain.https://doi.org/10.1371/journal.ppat.1011415.g005

EFig 6 . 1 V
Fig 6.Rvv regulon and its contribution to virulence.(A) RNA-seq analysis of ΔrvvA and wild-type strains grown under virulence-inducing conditions (AKI).Volcano plots display differential transcript abundance in ΔrvvA compared to wild type (n = 3).The negative log of the adjusted p-value (base 10) is plotted on the y axis, and the log of the fold-change (FC) (base 2) is plotted on the x axis.Each point represents a transcript.Gray lines indicate cutoffs for differential expression-a log2 FC with an absolute value greater than 1 (vertical lines), and an adjusted p-value less than 0.05 (horizontal line).A log 2 (fold change) > 0 indicates increased expression of a transcript in ΔrvvA compared to wild type.Differential coloring is used for transcripts of genes involved in biofilm formation (pink), pathogenesis (purple), or comprising the rvv loci (orangeyellow).A marginal distribution runs along the x-axis depicts the density of transcripts from a given pathway within the plot.(B) Volcano plots with subsets of the data shown in (A), displaying only transcripts of genes involved in biofilm formation (pink) and pathogenesis (purple).(C)-Genomic region representing rvvABCDE loci (VCA0258-VCA0254).(D)-Promoter activity of Prvv-lux transcriptional fusions from upstream regulatory regions of rvvA (triangles), rvvD (squares), and rvvE (diamonds) was measured from exponentially grown cells in wild type (blue) and ΔrvvA (orange).Individual data points of Relative Luminescence Units (RLU) are overlaid on top of crossbars displaying the mean and standard deviation.For each transcriptional fusion, means from at least three biological replicates were compared by an unpaired t-test.*, P � 0.01; **, P � 0.001; ***, P � 0.0001.(E)-The competitive index (CI) of indicated strains to colonize the infant mouse intestine were analyzed using a competition assay with an isogenic wild-type strain.Each data point represents the CI in an individual mouse.Statistical significance was determined using a One-Way ANOVA and post-hoc Tukey's multiple comparisons test.Means from individual biological replicates (n � 6) were compared to that of ΔrvvA, and differences with an adjusted P value of � 0.01 were deemed significant.***, P � 0.0001; ns, not significant.https://doi.org/10.1371/journal.ppat.1011415.g006

Fig 8 .
Fig 8. Model of Rvv TCS signal transduction.In the wild-type background, deletion of the gene encoding the sensor histidine kinase RvvA increases transcription of the rvv loci.Increased rvv transcription is dependent on both the presence and phosphorylation-state of the response regulator RvvB.However, RvvB D57E is not sufficient to increase target gene transcription unless RvvA is lacking.This suggests that rvv transcription might be repressed by an unknown repressor that requires the presence of RvvA, or that RvvA is inhibiting a key protein/signal needed for the function/activation of RvvB and thus presenting RvvB associated phenotypes.A strain harboring RvvA T293A , a mutated form of RvvA that abolishes phosphatase function, phenocopies ΔrvvA, supporting a model where RvvA acts as a phosphatase on RvvB.Under the conditions tested, RvvB phosphorylation may be mediated by acetyl-phosphate or crosstalk with another HK.RvvA's kinase function may be activated in response to a specific signal, leading to RvvB phosphorylation.The impact of RvvC on RvvAB phosphotransfer and the identity of signals sensed by RvvA or RvvC, have yet to be determined.Increased transcription and phosphorylation of RvvB activates the Rvv TCS, increasing transcription of rvv loci and biofilm formation genes, and reducing in vivo fitness.https://doi.org/10.1371/journal.ppat.1011415.g008

7 ) D Fig 3. Epistasis analysis of rvv loci contributions to biofilm formation. Promoter
ΔvpsT, suggesting that VpsT contributes but is not required for RvvA effects.Together, these findings indicate that VpsR and VpsT function downstream of RvvA to control vpsL transcription.