Eosinophils mediate SIgA production triggered by TLR2 and TLR4 to control Ascaris suum infection in mice

Human ascariasis is the most prevalent but neglected tropical disease in the world, affecting approximately 450 million people. The initial phase of Ascaris infection is marked by larval migration from the host’s organs, causing mechanical injuries followed by an intense local inflammatory response, which is characterized mainly by neutrophil and eosinophil infiltration, especially in the lungs. During the pulmonary phase, the lesions induced by larval migration and excessive immune responses contribute to tissue remodeling marked by fibrosis and lung dysfunction. In this study, we investigated the relationship between SIgA levels and eosinophils. We found that TLR2 and TLR4 signaling induces eosinophils and promotes SIgA production during Ascaris suum infection. Therefore, control of parasite burden during the pulmonary phase of ascariasis involves eosinophil influx and subsequent promotion of SIgA levels. In addition, we also demonstrate that eosinophils also participate in the process of tissue remodeling after lung injury caused by larval migration, contributing to pulmonary fibrosis and dysfunction in re-infected mice. In conclusion, we postulate that eosinophils play a central role in mediating host innate and humoral immune responses by controlling parasite burden, tissue inflammation, and remodeling during Ascaris suum infection. Furthermore, we suggest that the use of probiotics can induce eosinophilia and SIgA production and contribute to controlling parasite burden and morbidity of helminthic diseases with pulmonary cycles.

Introduction airway diseases, especially because of the Th2 polarized response following primary infection [12,13] and further exacerbation by a mixed Th2/Th17 response following multiple exposures [9].
Migrating Ascaris larvae induce a robust local response in the lungs of experimentally infected mice. The likely source of interleukin (IL)-5, although not yet elucidated in ascariasis, appears to be type 2 innate lymphoid cells (ILC2s), as has been demonstrated in murine models of allergic asthma and other helminth infections [11,[14][15][16]. ILC2s respond to IL-25 and IL-33 cytokines derived mainly from epithelial cells in response to damage, and in turn, produce type 2 cytokines, such as IL-5, IL-9, and IL-13, and secrete IgE, IgG1, and IgA [16]. Eight days post-infection, at the peak of Ascaris larvae migration in the lungs is characterized by marked IL-6 production that may be related to a prominent neutrophil infiltration that occurs concomitantly. When the larvae start to leave the lungs and migrate to the small intestine, the neutrophil infiltrate in the lungs is replaced by both mononuclear cells and eosinophils [8]. Eosinophils are also implicated in the regulation and maintenance of alternatively activated macrophages, which further confirms the role of eosinophils as a critical regulator of type 2 responses [16].
Although eosinophils are considered important in anthelmintic immune responses [6,17], studies on eosinophil homeostatic functions have also demonstrated their participation in plasma cell survival and regulation of secretory IgA (SIgA) production in the intestinal mucosa [5,[18][19][20]. Studies on the parasite Teladorsagia circumcincta demonstrated that SIgA and eosinophil kinetics were similar and that the interaction between the two components of the immune response contributes to parasite size reduction [17]. Similarly, in experimental infections of Cooperia oncophora, eosinophils and SIgA were correlated with reduced parasite burden [21].
The effect of eosinophil absence on SIgA production has also been demonstrated using a GATA1 -/mouse model [18,19] wherein eosinophil absence leads to a reduction in antibody production in the intestinal mucosa. Therefore, in this study, we explored the role of eosinophils during larval ascariasis and investigated the relevance of eosinophils in SIgA production, parasite burden control, and tissue repair. Furthermore, we highlight the importance of TLR2 and TLR4 receptor signaling in eosinophil recruitment and SIgA production, resulting in effective cellular and humoral immune responses against larval ascariasis in mice.

Ethics statement
All procedures in this study were approved by the Ethics Committee for Animal Experimentation (CETEA) of Federal University of Minas Gerais (UFMG), Brazil (Protocol # 04/2012, Protocol # 054/2012, and Protocol # 187/2014). The animals were maintained in accordance with the recommendations of the Brazilian College of Animal Experimentation guidelines (COBEA).

Experimental design
The following four independent experimental designs were used in this study depending on the objective: 1) To evaluate the kinetics of the immunological and parasitological parameters, WT C57BL/6 and BALB/c mice were divided into seven groups-a control group = noninfected (NI) and six single-infected groups (SI). 2) To evaluate the role of eosinophils in TLRdeficient mice, immunological and parasitological parameters were observed at 8 dpi. The animals were divided into two groups-a control group = non-infected (NI) and a single-infected group (SI). 3) To evaluate the effect of probiotics on immunological and parasitological parameters, animals were divided into four groups-a non-infected group treated with placebo (NI-M17), a non-infected group treated with probiotics (NI-NCDO), a single-infected group treated with placebo (SI-M17), and a single-infected group treated with probiotic (SI-NCDO). 4) To evaluate the role of eosinophils in single and multiple infections, GATA1 -/and WT BALB/c mice were divided into three groups-a non-infected group (NI), single-infected group (SI), and re-infected group (RE). Mice from the NI groups were administered phosphate buffered saline (PBS) only. Mice from the SI group were treated with two doses of PBS followed by one dose of suspension containing 2500 fully embryonated eggs of A. suum (singleinfection), maintaining an interval of 14 days between doses. Mice from the RE group were administered three doses of a suspension containing 2500 fully embryonated eggs of A. suum, maintaining an interval of 14 days between doses (re-infection). 5) To evaluate the participation of eosinophils in probiotic-mediated SIgA modulation, GATA1 -/and WT BALB/c mice were divided into four groups-a treated with placebo and non-infected (PBS NI), treated with placebo and infected with A. suum (PBS SI), treated with NCDO (2118) and non-infected, and, treated with NCDO (2118) and infected with A. suum (NCDO SI). Tissue harvesting for parasitological and immunological evaluation was performed as previously described [9] at all time points of kinetics experiments or following the peak migration of larvae in the liver, lungs, and intestine, which were observed on days 4, 8, and 12 of infection, respectively [8]. The experimental design and results are outlined in the figures.

Bacterial growth conditions for probiotic diet
A gram-positive and non-invasive bacterial strain from Lactococcus lactis subsp. lactis (NCDO 2118) was used to assess the impact of a probiotic diet on ascariasis. Bacteria were grown as previously described [22], with some modifications. First, for experimental design 3, bacteria were cultured for 18 to 21 hours, at 30˚C in M-17 broth medium (Sigma) supplemented with 0.5% glucose and 10 μg/mL of chloramphenicol (Sigma) without agitation. Each animal was given a volume of 5 mL containing either M17 medium (group M17) or freshly cultured bacteria in the stationary growth phase (NCDO group) daily. An estimated dose of 1 × 10 9 CFU/mL bacteria per day for 22 days was ingested by each mouse [22].
For experimental design 5, the bacterial culture was prepared similarly, but without the use of antibiotics. Upon reaching the stationary phase, the culture was centrifuged at 4000 g at 4˚C for 10 minutes. The supernatant was discarded, and the pellet was resuspended in PBS. This solution was administered in mice intragastrically, with the aid of a gavage needle, at a concentration of 1 × 10 9 CFU/100μL bacteria per day for 22 days [23].
During the experimental period, the mice had unlimited access to food and water.
incubated with 0.2 M H 2 SO 4 for embryonation, as described by Boes et al. [24]. After the 100 th day of culture, which corresponds to the peak of larval infectivity [8], fully embryonated eggs were used in experimental infections and some were allowed to hatch for harvesting larvae to obtain antigens.

Experimental infection and parasitological analysis
The mice were administered either 200 μL of PBS or 2500 embryonated eggs suspended in 200 μL PBS using a gavage needle, according to the group, time point, and experimental design. To assess the parasite burden, tissues were collected, sliced using scissors, and placed in a Baermann apparatus for 4 hours in PBS at 37˚C. The recovered larvae were then fixed with 1% formaldehyde in PBS and counted under an optical microscope. Total parasite burden in the kinetic experiment was assessed as the total number of larvae recovered from the liver, lungs, and intestines at all six time points. In all other experimental designs, the parasite burden was assessed as the number of larvae recovered from the liver, lungs (parenchyma + airways), or intestine, separately.

Attainment and analysis of bronchoalveolar lavage (BAL)
Bronchoalveolar lavage (BAL) was obtained and used for the analyses of cellularity, blood loss, protein leakage, SIgA levels in the BAL, and the number of larvae in the airways. For obtaining BAL, the mice were administered a lethal dose of anesthetic (xylazine 45 mg/kg and ketamine 120 mg/kg). The animals were then tracheostomized and a 1.7 mm catheter was inserted into the trachea to obtain the BAL through two infusions and aspirations using 1 mL of PBS. The BAL obtained was filtered through 40 μm cell filters (BD, USA) to recover A. suum larvae, which were used to assess the parasite burden in the airways [9]. Filtered BAL was centrifuged at 3000 g for 10 minutes and the pellet was used to determine the total number of leukocytes and their subpopulations (macrophages, lymphocytes, eosinophils, and neutrophils) using optical microscopy. The supernatant was used to quantify blood loss, protein leakage, and SIgA levels by ELISA.

Assessment of respiratory mechanics
For assessing respiratory mechanics, mice were anesthetized using subcutaneous ketamine and xylazine injection (8.5 mg/kg xylazine and 130 mg/kg ketamine) to maintain spontaneous breathing under anesthesia, following which they were tracheostomized, placed in a body plethysmograph, and connected to a computer-controlled ventilator (Forced Pulmonary Maneuver System, Buxco Research Systems, Wilmington, North Carolina USA), as previously described by Nogueira and colleagues (2016) [9]. An average breathing frequency of 160 breaths/min was imposed on the anesthetized animal by pressure-controlled ventilation until a regular breathing pattern and complete expiration at each breathing cycle was obtained. Under mechanical respiration, the dynamic compliance (Cdyn) and lung resistance (Rl) were determined using resistance and compliance RC tests.

Histopathological analysis
For histopathological analysis after single-infection, mice were euthanized and the left lobe of the lung was dissected, fixed in 10% buffered formalin (pH 7.2), and embedded in paraffin. Tissue sections (5 μm thick) were mounted on glass slides and stained with hematoxylin and eosin (H&E) for inflammation analysis, Masson's trichrome or Picrosirius Red for quantification of collagen neoformation as well as determination of the type of collagen fiber under polarized light illumination. The tissues were then imaged using a standard light microscope (Leica DM5000). For studying the morphometric parameters of inflammation, the images were analyzed from 15 randomly selected fields (total area 1.12 × 10 6 μm 2 ) on a single slide per animal. The segmentation allowed us to select the nuclei of all cell types present in the lung and to exclude the cell cytoplasm and other structures present in the histological sections. Using this process, a binary image was created, and the nuclei of all cell types present in the lung were counted. The difference between the number of cell nuclei present in infected mice and that in non-infected animals was considered as the number of inflammatory cells. For studying the morphometric of collagen neoformation, the images were analyzed from 20 randomly selected fields (total area 1.5 × 10 6 μm 2 ) on a single slide per animal. Collagen neoformation in the lung was quantified by measuring the collagenized areas present in the sections, and the difference between the collagenized areas in the infected and non-infected mice was considered as fibrosis process. All sections were viewed using a 40X objective and the images were digitized using a Leica DFC340FX microcamera associated with Leica DM5000B microscopy and analyzed using the image processing and analysis software Leica Qwin V3 (Leica Microsystems, Wetzlar, Germany). For histopathological analyses after re-infection experiments, mice were euthanized on the 8 th day after infection, and their lungs were removed. The left lobe was fixed in a 10% solution of formaldehyde (Synth, Brazil) in PBS for 72 hours. After processing in alcohol and xylol, tissue fragments were embedded in paraffin and 4 μm thick sections were obtained and stained with H&E for morphometric quantification of inflammatory and Gomori's trichrome for morphometric quantification of collagen deposition and fibrosis. Images from the tissue sections were collected using a 20X objective of the Axiolab-Carl Zeiss microscope (Oberkochen, Germany) and a Samsung SDC-415 micro-camera (Seoul, South Korea). Forty random images were captured with a 20X magnification lens, covering an area of 3.2 × 10 6 μm 2 of lung analyzed. The KS400 software was used to select all pixels of the lung tissue in the real image for creating a binary image, digital processing, and calculation of the area in μm 2 of the interalveolar septum and fibrosis [25].

Tissue IgA immunostaining
To further evaluate the expression of lung IgA, we performed immunohistochemistry as previously described [26]. Briefly, tissue sections were deparaffinized, hydrated, and washed in PBS (pH 7.2). Endogenous peroxidase activity was eliminated by treatment with H 2 O 2 40 v/v solution in 0.2% PBS and non-specific binding was blocked by incubation with goat serum diluted at 1:40. The sections were then incubated with polyclonal anti-IgA (Southern Biotechnology Associates, Birmingham, USA) diluted at 1:60 for 18 hours, and then incubated with biotinylated rabbit anti-goat IgG at 1:150 (Bethyl Laboratories Inc., Montgomery, USA). The reaction was then revealed using DAB 0.05% in H 2 O 2 40% v/v in 0.2% PBS. PBS-treated sections were used as negative controls (S1 Fig). All sections were stained with H&E, desihydrated, and diaphanized for entellan (Meck Millipore) assembly.

Adult worm and infective larvae antigen production
A. suum adult worm and larval antigen production was performed as described previously [27]. Briefly, larvae antigen was used to induce the hatching of fully embryonated A. suum eggs, a standard protocol for Toxocara canis was used with some modifications [28]. Embryonated A. suum eggs were incubated in RPMI-1640 medium (SIGMA, USA; pH 7.2) supplemented with 4% penicillin/streptomycin (Invitrogen, USA), and placed in 24-well plates for larval hatching. To produce L3 larvae antigen, purified larvae were collected and transferred to a 50 mL graduated tube, centrifuged at 800 g for 10 minutes at room temperature. The supernatant was discarded and the pellet was resuspended in 5 mL of PBS, and then sonicated (Cole Parmer Ultrasonic Homogenizer Power Supply 4710 Series, USA) at 60 W for 1 minute, with a 30-second interval for each cycle, totaling 10 cycles. After sonication, the extract was centrifuged at 800 g for 15 minutes at 4˚C. The supernatant was then collected and stored at -80˚C until use. The amount of protein in all antigenic extracts was measured using a commercial BCA protein assay kit (Thermo Fisher Scientific, USA), according to the manufacturer's instructions.
Adult worm antigens were initially obtained by mechanical maceration of the parasites in PBS and then using a sonicator (Cole Parmer Ultrasonic Homogenizer Power Supply 4710 Series, USA). The macerated extract was kept under ice-cooling and then sonicated at 60 W for 1 minute, with a 30-second interval for each cycle, totaling five cycles. Finally, the protocol was performed as previously described for larval antigens.

Cytokine profile
To determine the cytokine profile in the lungs, the lungs were homogenized using a tissue homogenizer (Power Gen 125 -Fisher Scientific Pennsylvania, USA) in 100 mL of PBS supplemented with protease inhibitors (0.1 mM phenylmethylsulfonyl fluoride (PMSF), 0.1 mM benzethonium chloride, 10 mM EDTA and 20 KI aprotinin A) and 0.05% Tween 20. The lung homogenates were then centrifuged at 8000 g for 10 minutes at 4˚C and the supernatant was used to determine cytokine production. Production of IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, and TNF-α was assessed by flow cytometry (Th1/Th2/Th17 Cytometric Bead Array, BD Biosciences, USA) using a FACScan (BD Biosciences, USA) according to the manufacturer's recommendations. IL-5, TGF-β, and IL-13 levels were measured using a sandwich ELISA kit (R&D Systems, USA) according to the manufacturer's instructions. Finally, the intensity of the reaction was determined at a wavelength of 492 nm using an automated reader (VersaMax Tunable Microplate Reader, Molecular Devices), and the cytokine concentration (pg/mL) for each sample was calculated by interpolation using a standard curve fitted using the fiveparameter logistic (5-PL).

Eosinophil peroxidase and neutrophil myeloperoxidase assays
Eosinophil peroxidase (EPO) and neutrophil myeloperoxidase (MPO) activity in lung homogenates were measured according to the method described by Strath and modified by Silveira [29,30]. The tissues were homogenized (Power Gen 125-Fisher Scientific Pennsylvania, USA), the homogenate was centrifuged at 8000 g for 10 minutes at 4˚C, and the pellet obtained was used to determine EPO and MPO activity. For the EPO assay, the pellet was homogenized in 950 μL PBS and 0.5% hexadecyltrimethylammonium bromide (Sigma Chemical Co, St. Louis, MO, USA), and then frozen-thawed three times using liquid nitrogen. The lysate was then centrifuged (1500 g, 4˚C, 10 minutes) and the supernatant was distributed in a 96-well microplate (75 μL/well) (Corning, USA), followed by the addition of substrate (1.5 mM OPD and 6.6 mM H 2 O 2 in 0.05 M Tris-HCl, pH 8.0) (75 μL/well), and incubation for 30 minutes at room temperature. The reaction was stopped by the addition of 50 μL of 1 M H 2 SO 4 and absorbance was determined at 492 nm.
For the MPO assay, the pellet obtained after centrifugation was homogenized in 200 μL of buffer 1 solution (0.1 M NaCl, 0.02 M Na 3 PO 4 , 0.015 M Na 2 EDTA, pH 4.7), then centrifuged (1500 g, 4˚C, 10 minutes). Then, 800 μL of buffer 2 solution (0.05 M NaPO 4 , 0.5% hexadecyltrimethylammonium bromide) was added to the pellet and this mixture was homogenized and then frozen-thawed three times using liquid nitrogen. The lysate was centrifuged (1500 g, 4˚C, 10 minutes) and the supernatant was used for the enzymatic assay. To each well of a 96-well microplate, 25 μL of supernatant was distributed (Corning, USA) followed by the addition of 25 μL of substrate TMB (3.3'-5.5-tetramethylbenzidine + 1.6 mM dimethylsulfoxide), and 100 μL of 0.5 M H 2 O 2 and incubated for 5 minutes at room temperature. The reaction was stopped by adding 100 μL of sulfuric acid (1 M H 2 SO 4 ). Finally, the intensity of the reaction was determined at a wavelength of 450 nm using an automated reader (VersaMax Tunable Microplate Reader, Molecular Devices).
For quality control, positive and negative controls were included on all plates. Serial dilutions of IgA standards (Mouse IgA standard, Southern Biotechnology) were used in all complete total SIgA evaluation plates. Concentrations of total SIgA (μg/mL) were determined by interpolation of the sample optical density (OD) in a standard curve fitted using the fiveparameter logistic (5-PL). Levels of Ascaris-specific Igs are expressed as OD.

Statistical analyses
Statistical analyses were performed using the GraphPad Prism 8 software (GraphPad Inc., USA). The Grubb's test was used to detect possible outliers in the groups. The Mann-Whitney

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Eosinophils mediate ascariasis control test was used for comparing parasite burden and the areas of lesions in the liver and lungs. One-way ANOVA followed by Tukey´s multiple comparisons test was used to evaluate differences between groups in kinetics experiments. Pearson's Linear Regression was used to evaluate the correlation. Data from EPO and MPO assays and pulmonary mechanics, hemoglobin, protein levels, and BAL cellularity were analyzed using the Kruskal-Wallis test followed by Dunn's test. Finally, a two-way ANOVA with multiple comparisons was performed to assess differences between the groups as a function of time. All values were considered significant when at p � 0.05.

Mucosal SIgA production correlates with eosinophilia and controls parasite burden during larval ascariasis in mice
The relationship between eosinophils and SIgA in A. suum infection model was initially evaluated by comparing immune response at different timeframes during larval migration kinetics in WT BALB/c and WT C57BL/6 mice. Immune responses associated with larval migration were assessed based on leukocyte influx in the lungs and SIgA production. After A. suum infection, there was a progressive increase in the total number of leukocytes, macrophages, lymphocytes, and eosinophils from the 8 th dpi to 12 th dpi as larvae migrate through the lungs. Whereas the number of neutrophils increased on the 8 th dpi but decreased on the 10 th and 12 th dpi ( Fig 1A and S1 Table). Interestingly, we found similar kinetics of eosinophil influx ( Fig 1B) and total SIgA levels ( Fig 1C) in the lung mucosa, with a progressive increase after the 6 th dpi. This correlation suggests that eosinophils contribute to control of the parasite burden and is relevant to SIgA production during larval ascariasis in mice. In mouse airways, increased SIgA levels on the 8 th dpi was positively correlated with eosinophil influx ( Fig 1D) and negatively correlated with the number of larvae ( Fig 1E). Fig 1F shows the experimental design. WT C57BL/6 and WT BALB/c mice were divided into non-infected (n = 20) and single-infected (n = 84) groups. The data in Fig 1 represent the mean of the results of the two strains, as there were no significant differences in the responses observed between the two strains.
Apart from our objective, we also verified that there was a significant increase in the production of IgM in the BAL and serum of WT C57BL/6 mice on the 10 th dpi. However, there was no significant difference between WT BALB/c and WT C57BL/6 strains (S2 Fig).

TLR2 and TLR4 mediates mucosal SIgA production and eosinophilia that controls parasite burden during larval ascariasis in mice
After demonstrating the relationship between SIgA levels and eosinophils controlling larval migration, and after verifying the increase in the expression of TLR2 and TLR4 in singleinfected mice (S3 Fig), we assessed the potential role of TLRs in triggering SIgA production and eosinophilia. Thus, we investigated the immune response and parasite burden during A. suum infection in mice deficient for Toll-like receptor 2 or Toll-like receptor 4 (TLR2 -/or TLR4 -/-) (Fig 2A). We found that both TLR2 and TLR4 receptors are required to induce a protective immune response, as a significant reduction in eosinophil numbers in the airways was observed in TLR2 -/and TLR4 -/mice ( Fig 2B) compared to WT mice. Furthermore, we also found lower levels of total SIgA in BAL and intestine lavage from TLR2 -/and TLR4 -/deficient mice, compared to that of WT mice (Fig 2C and 2D). This reduction in immune response was due to a significant increase in the number of Ascaris larvae recovered from lung tissue and airways from TLR2 -/and TLR4 -/mice. The most marked burden occurred in the airways of TLR4 -/mice (Fig 2E), suggesting that TLR deficiency also affects the number of larvae with the potential to complete their life cycle in the intestine.
Considering the relevant role of TLRs in A. suum infection, we investigated the use of probiotics in inducing a response mediated by TLR receptors in the course of infection. The bacterium Lactococcus lactis (NCDO 2118) is a gram-positive bacterium that has lipoteichoic acid (LTA) on its surface. These PRRs are conserved molecular structures that can be recognized by TLR2 [31]. We found an increase in SIgA production and the number of eosinophils, as well as a concomitant reduction in parasite burden at pulmonary sites in mice treated with the probiotic bacteria during infection with A. suum when compared to mice that received the placebo (M17) (Fig 2F). This finding suggests that treatment with probiotics may stimulate receptors responsible for modulating eosinophilia and SIgA levels, which have a role in parasite

Eosinophils are required for mucosal SIgA production and parasite burden control during larval ascariasis in mice
To further investigate the role of eosinophils in SIgA production during larval ascariasis, we evaluated the immune response to Ascaris infection in non-eosinophilic mice (GATA1 -/-) and compared it to that of wild-type mice (WT BALB/c) ( Fig 3A). The absence of eosinophils leads to the loss of parasite burden control in GATA1 -/mice ( Fig 3B). The role of eosinophils in regulating the levels of total and A. suum antigen-specific SIgA was established by the increase in eosinophils in WT BALB/c mice BAL, which was not observed in GATA1 -/mice ( Fig 3C). BAL from WT BALB/c and GATA1 -/mice showed a significant increase in total SIgA levels on the 8 th dpi, however, this increase was A. suum antigen-specific in only WT BALB/c mice. This demonstrates the importance of eosinophils in the control of parasites ( Fig 3B) and reinforces the important role of eosinophils in the modulation of the humoral immune response during A. suum infection in mice.

Eosinophils are essential in modulating SIgA production during Ascaris suum infection
To evaluate the role of eosinophils in SIgA modulation after treatment with probiotic L. lactis (NCDO 2118), GATA1 -/and WT BALB/c mice were treated for 22 days with probiotic and infected with A. suum ( Fig 4A). We found that GATA1 -/-NI mice treated with placebo showed high levels of total SIgA in the BAL when compared to WT BALB/c NI and GATA1 -/-SI group mice treated with placebo. Interestingly, GATA1 -/-SI mice treated with placebo showed lower total SIgA levels ( Fig 4B and S2 Table).
We found an increase in total SIgA levels in the WT BALB/c NI group treated with NCDO than in the WT BALB/c NI group treated with placebo. This indicates the efficiency of L. lactis in inducing the production of total SIgA. Finally, our findings showed that GATA1 -/-SI mice treated with NCDO had higher levels of total SIgA than GATA1 -/-SI mice treated with placebo, suggesting that probiotic treatment contributes to the maintenance of high levels of total SIgA in the BAL in the absence of eosinophils (Fig 4B and S2 Table).
By analyzing the parasite burden on the 22 nd day after treatment with probiotics and 8 dpi (Fig 4C), we found that GATA1 -/-SI mice treated with placebo showed higher parasite burden than in WT BALB/c SI mice treated with placebo. Whereas GATA1 -/mice treated with NCDO showed reduced parasite burden than GATA1 -/mice treated with placebo ( Fig 4C). Interestingly, no significant differences were observed between wild-type mice.
Together, these results confirm that in the absence of eosinophils, the ability of mice to control parasite burden as well as post-infection SIgA levels reduces. However, the absence of BAL (n = 14) (C) and intestine lavage (n = 14) (D) on the 8 th dpi. Total parasite burden, that is, the number of larvae recovered in lung parenchyma and airways of WT C57BL/6, TLR2 -/and TLR4 -/mice on the 8 th dpi (n = 14) (E). Effect of probiotic Lactococcus lactis (NCDO 2118) on immunological and parasitological parameters during the acute phase of experimental ascariasis in mice, namely, number of eosinophils in BAL (n = 7), total SIgA (n = 7), and coincident reduction in parasite burden (n = 7) (F). Two-way ANOVA followed by Sidak´s multiple comparisons test was used to evaluate differences between groups (B-E) and the Mann-Whitney test was used to assess differences between the groups (F). Differences were considered significant at p � 0.05 and are represented by symbols in the graphs. � represents differences between knockout and WT C57BL/6 mice groups and # represents differences between non-infected and single-infected groups from same strain (B, C, and D); and, the � represents differences between the single-infected and non-infected group and & represents differences between the NCDO and M17 groups (F

Eosinophils are associated with inflammation, tissue remodeling, and pulmonary dysfunction caused by larval migration during A. suum singleinfection
In addition to contributing to the control of parasite burden, eosinophils are important in pulmonary pathophysiology during larval ascariasis, and consequently, fibrosis modulation [10]. Therefore, the role of eosinophils in the modulation of pulmonary inflammation following . T-test was used to assess differences between the groups in (B); one-way ANOVA followed by Tukey´s multiple comparisons test was used to evaluate differences between groups in (C). Differences were considered significant at p � 0.05 and are represented by symbols in the graphs. � represents differences between the single-and non-infected group's mice of the same strains, and & represents differences between the WT and GATA1 -/mice with the same treatment [

Eosinophils mediate ascariasis control
Ascaris infection was assessed through the number of innate cells, cytokine profile, and eosinophil and neutrophil activity in lung tissue between non-infected and single-infected WT BALB/c and GATA1 -/mice. Qualitative analyses of leukocyte influx into the lungs during larval migration in WT BALB/c mice revealed neutrophils to the most abundant at the peak of larval migration (8 th dpi). However, eventually (12 th dpi), the inflammatory infiltrate was more pronounced, mostly composed of mononuclear cells and eosinophils (Fig 5A). Similarly, quantitative morphometric analyses confirmed that Ascaris larval migration induced significant pulmonary cell infiltration on the 8 th and 12 th dpi (243 ± 48 vs. 484 ± 95cells/74588.6 μm 2 of tissue) (Fig 5B), with progressive accumulation of eosinophils up to the 12 th dpi (Fig 5C). In contrast, GATA1 -/mice showed a significant reduction in the number of leukocytes in the airways ( Fig 5D) and a reduction in inflammatory cell accumulation in the lung tissues at 12 th dpi than in WT BALB/c mice (330 ± 20 vs. 484 ± 95cells/74588.6 μm 2 of tissue). . Differences between groups were statistically evaluated by the following tests: Two-Way ANOVA followed by Sidak´s multiple comparisons test was used to evaluate differences between groups in (B); One-Way ANOVA followed by Tukey´s multiple comparisons test was used to evaluate differences between groups in (C). Differences were considered significant at p � 0.05 and are represented by symbols in the graph (C) where the � = p < 0.05. Data are presented as mean ± SEM. https://doi.org/10.1371/journal.ppat.1010067.g004

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Eosinophils mediate ascariasis control

Eosinophils mediate ascariasis control
The results revealed that larval migration in lungs induced a marked increase in cytokine and granule production in the pulmonary parenchyma on the 12 th dpi in WT BALB/c mice than in non-infected mice (Fig 5E-5Q). However, GATA1 -/mice showed a marked ablation of EPO (Fig 5G) and reduced MPO levels (Fig 5F), mainly on the 12 th dpi than in singleinfected WT BALB/c mice. In addition, at the 12 th dpi, there was a significant increase in IL-4, IL-10, IFN-γ, and TNF-α levels (Fig 5L, 5N, 5P, and 5Q) in single-infected WT BALB/c group but not in single-infected GATA1 -/mice. Whereas an increase in IL-5 and IL-13 was observed mainly in GATA1 -/-SI mice (Fig 5K and 5M). Collectively, our data support that eosinophils contribute to lung inflammation during larval ascariasis in mice.
Despite the increased levels of mediators, such as IL-4 and IL-13, we further investigated tissue fibrosis and pulmonary mechanical dysfunction. Histological analysis performed at the 8 th dpi showed normal collagen levels in the lung tissue of A. suum single-infected GATA1 -/and WT BALB/c mice, while tissue fibrosis was observed at the 12 th dpi in both groups. (Fig 6A-6D). Tissue remodeling-induced larval migration lead to pulmonary dysfunction, which was more pronounced in GATA1 -/mice than in WT BALB/c mice. We observed that larval migration through the lungs induced a significant reduction in pulmonary volumes, as depicted by (FVC), inspiratory capacity (IC), and forced expiratory volume (FEV100) at 8 th dpi with partial recovery at 12 th dpi in WT BALB/c mice (Fig 6E, 6H and 6I); however, GATA1 -/mice showed a marked decline in pulmonary volumes at 12th dpi (Fig 6E and 6I). Similarly, increase in lung resistance (Rl) and dynamic compliance (Cdyn) decline revealed that GATA1 -/infected mice¸compared to WT infected mice, showed a progressive loss in pulmonary elasticity (Fig 6F and 6G), suggesting that excessive larval migration could induce increased tissue damage and trigger excessive fibrosis.
To further characterize the predominance of collagen types in lung tissue, we used Masson's trichrome staining and Picrosirius Red, with or without polarized light. We observed a normal histological appearance with few collagen fibers in both WT BALB/c and GATA1 -/mice groups at the 8 th dpi (Fig 6J and 6K). Interestingly, an increase in collagen deposition was observed in single-infected WT BALB/c mice at the 12 th dpi ( Fig 6L) and was even more pronounced in GATA1 -/mice at the 12 th dpi (Fig 6M). However, analysis with polarized light on Picrosirius Red showed increased deposition of type I collagen at the 12 th dpi (stained red, Fig  6P and 6Q) instead of type III collagen observed at the 8 th dpi (stained green, Fig 6N and 6O).

Eosinophils contribute to parasite burden control and SIgA production during larval ascariasis after multiple exposures
Having highlighted the importance of SIgA in controlling parasite burden as well as its correlation with the number of eosinophils, we used a re-infection model previously established by our group [9], which provided evidence of protection after re-infection. Thus, in the experimental design, we compared single-infected (SI) WT BALB/c and GATA1 -/groups to reinfected (RE) WT BALB/c and GATA1 -/groups (Fig 7A) at the 8 th dpi, which correspond to Representative microphotography of lung tissue stained with H&E (Bar scale = 50 μm) (A-D). Number of total leukocytes recovered from BAL on the 8 th dpi (n = 6) (E). Number of eosinophils in situ in the lung at 0, 4, 8 and 12 dpi (n = 5) (F). Optical density representing Eosinophil peroxidase (EPO) and neutrophil myeloperoxidase (MPO) activity in the lung at 0, 4, 8, and 12 dpi (n = 12) (G and H). Levels of cytokines in the lung at the 12 th dpi (n = 6) (I-Q). Data are represented as mean ± SEM. One-way ANOVA followed by Tukey´s multiple comparisons test was used to evaluate differences between groups (G, I, J, K, L, N, O, and P). Kruskal-Wallis test followed by Dunn´s multiple comparisons test was used to evaluate differences between groups (H, M, and Q). The p values are represented by symbols in the graphs wherein � represents differences between the non-infected group, � represents differences between same groups in the 8 dpi, # represents differences between groups from different strains that received the same treatment. [

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Eosinophils mediate ascariasis control Data are represented as mean ± SEM. Significant differences between GATA1 -/and WT BALB/c groups at the 12 th dpi are represented in the graph by p values. P values are represented by symbols in the graphs wherein � represents differences between non-infected groups from the same strain, � represents differences between the

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Eosinophils mediate ascariasis control the pulmonary phase of Ascaris larval migration. Initially, we observe decreased parasite burden in WT BALB/c re-infected mice. We found that eosinophil deficiency in GATA1 -/mice in both single-and re-infected groups reduced parasite burden control when compared to the WT BALB/c groups (Fig 7B). Furthermore, parasite numbers in different lung compartments (airways and tissue), showed that more larvae transmigrated into the airways of GATA1 -/than in the WT BALB/c re-infected group (Fig 7B), suggesting that eosinophils may control the parasite cycle in mice.
We found no eosinophils in the BAL in GATA1 -/mice, whereas the WT BALB/c reinfected group showed a significant increase in eosinophils (Fig 7C). In addition, re-infected WT BALB/c and GATA1 -/mice showed a significant increase in the total number of leukocytes, macrophages, neutrophils, and lymphocytes than NI and SI BALB/c and GATA1 -/mice. However, the increase in lymphocyte numbers was more accentuated in the GATA1 -/-single-infected groups from the same strain, # represents differences between groups from different strains that received the same treatment. One-way ANOVA followed by Tukey´s multiple comparisons test was used to evaluate differences between groups. [ 14) (B). Number of total leukocytes and their subpopulations, namely, lymphocytes, macrophages, neutrophils, eosinophils, and total protein and hemoglobin levels in BAL (n = 6) (C). All immunological and parasitological parameters were evaluated at the 8 th dpi. Data are represented as mean ± SEM. Significant differences (p < 0.05) between are represented in the graph by p symbols. � represents differences between non-infected groups from the same strain, � represents differences between the singleinfected groups from the same strain, # represents differences between groups from different strains that received the same treatment. [

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Eosinophils mediate ascariasis control re-infected group (Fig 7C). BAL evaluation also showed increased levels of total protein and hemoglobin in the airways of single-and re-infected WT BALB/c and GATA1 -/mice but was more pronounced in the GATA1 -/single-infected group (Fig 7C). There was a positive correlation between total protein and hemoglobin levels in the airways (r 2 = 0.666, p = 0.0001), probably due to hemorrhage caused by larval migration.
The relevance of eosinophils and SIgA levels in the lung mucosa and their ability to control parasite burden was more evident in the re-infection model, as in contrast to high levels of total and A. suum antigen-specific SIgA levels (against antigens from adult worms and larvae of A. suum) in WT infected mice, the total and specific SIgA levels remained low in eosinophil-deficient mice (GATA1 -/-) even after re-infection (Fig 8A). To reinforce this relationship, we assessed lung tissue of mice for IgA-positive cells and found A. suum induced high IgA immunostaining, predominantly in the peribronchial spaces and in the bronchial epithelium to be more prominent in re-infected WT BALB/c mice (Fig 8F) than in single-infected WT BALB/c mice (Fig 8D). The single-infected ( Fig 8E) and re-infected GATA1 -/mice (Fig 8G) showed little to no staining.
Furthermore, we measured specific antibodies against Ascaris-antigens IgM, IgG1, IgG2A, IgG2B, and IgG3 and found that reinfected WT BALB/c and GATA1 -/mice showed a significant increase in the serum antibodies, indicating that the absence of eosinophils did not compromise specific antibody production (S4 Fig). These data suggest that despite increased production of IgM, IgG1, IgG2A, IgG2B, and IgG3 antibodies, the absence of eosinophils and reduction in SIgA compromised the infection control. Specific IgE levels were not detected.

Eosinophils are critical for chronic pulmonary inflammation and dysfunction recovery after multiple parasite exposure in mice
Evaluation of tissue injury caused by larval migration and lung inflammation revealed that all infected animals (subjected to single and multiple infections) from both mouse strains presented microscopic lesions in the parenchyma characterized by septal thickening, hemorrhage, vascular hyperemia, perivascular edema, and inflammation of the pulmonary parenchyma (Fig 9A-9H). However, these findings were more prominent in re-infected groups regardless of mouse strain, corroborating the results of morphometric evaluations of the lesion area. The lesion area did not differ between groups that received the same treatment, however, lesion characteristics differed between groups. Hemorrhage was more pronounced in single-infected groups from both mouse strains than in the GATA1 -/mice re-infected group, reinforcing that the injuries caused by larval migration are responsible for increased hemorrhage in the lungs. The inflammatory infiltrate was more prominent in the WT BALB/c and GATA1 -/re-infected groups. However, re-infected WT BALB/c mice showed mixed inflammatory infiltrate (with neutrophil and eosinophil predominance). These findings corroborate the finding of EPO and MPO assays that showed a significant increase in eosinophil and neutrophil activity in WT BALB/c RE mice when compared to the other groups (Fig 9I). Single-and re-infected GATA1 -/mice had blunted EPO levels and reduced MPO levels from tissue neutrophils and discrete bronchus-associated lymphoid tissues (BALT) formation.
Th1/Th2/Th17 type cytokines were measured to further characterize lung inflammation (Fig 9J). We found a significant increase in IL-2, IL-4, IL-10, and IFN-γ in the WT BALB/c single-and re-infected groups when compared to non-infected mice, but not in the GATA1 -/single-and re-infected groups. Inflammatory cytokines, such as IL-6, IL-17A, and TNF, were increased in WT BALB/c and GATA1 -/single-and re-infected groups, when compared to the respective non-infected groups. IL-5 levels were significantly increased in the GATA1 -/and WT BALB/c single-infected mice, but more pronounced in GATA1 -/single-infected mice. No

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Eosinophils mediate ascariasis control

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Eosinophils mediate ascariasis control differences in IL-13 and TGF-β1 levels were observed between WT and GATA1 -/single-and re-infected mice (Fig 9J).
Next, we evaluated pulmonary fibrosis in WT BALB/c and GATA1 -/mice in the single and re-infected groups (Fig 10). Histopathological evaluation of lung tissue sections stained with Gomori's trichrome was performed to determine the chronic tissue remodeling and fibrosis induced by A. suum larvae migration. Fibrosis was evident in some areas of alveolar septa, bronchi, bronchioles, and pulmonary blood vessels, which contributed to the thickening of the alveolar septum in single-infected and re-infected mice from both strains (Fig 10) but was more pronounced in re-infected WT BALB/c mice (Fig 10C and 10F). Re-infected GATA1 -/mice showed larger injury areas than GATA1 -/single-infected mice (Fig 10B, 10D, and 10E). Interestingly, corroborating the findings of morphometric analyses, re-infected WT BALB/c mice exhibited large areas of consolidated fibrosis, causing considerable thickening of the alveolar septa, and compromising a larger number of bronchi, bronchioles, and vessels than that observed in GATA1 -/mice (Fig 10C-10F). Invasive spirometry confirmed pulmonary lesions caused by larval migration, inflammation, and fibrosis, which were reflected in pulmonary mechanical dysfunction (Fig 10G). Re-infected WT BALB/c mice did not show worsening lung resistance and dynamic compliance (Fig 10C, 10F and 10G), however, re-infected GATA1 -/mice showed an increase in lung resistance and decreased dynamic compliance resulting from chronic pulmonary inflammation (Fig 10D-10G).

Discussion
Ascariasis is the most prevalent neglected tropical disease, affecting approximately 450 million people worldwide [2,4]. In ascariasis, larval migration through the host's organs leads to tissue injury followed by the induction of innate and adaptive immune responses, which are characterized by the production of Th2 and Th17 cytokines by lymphocytes, inflammatory cell infiltration of mainly neutrophils and eosinophils, and immunoglobulin induction against the parasite [8,9]. In this study, we investigated the role of eosinophils and adaptive immune responses during single or multiple larval ascariasis in mice. Our results collectively highlight the complex network involving innate and humoral immune responses that control the parasite burden during Ascaris suum infection in mice, and can be summarized as follows: (i) eosinophilia correlates with mucosal SIgA induction and parasite burden, (ii) TLR2 and TLR4 activation mediates eosinophilia and mucosal SIgA production, controlling parasite burden, (iii) eosinophils are required for SIgA production and parasite burden control; (iv) eosinophils are associated with inflammation, remodeling, and pulmonary dysfunction caused by larval migration, (v) eosinophils contribute to parasite burden control and SIgA production after multiple parasite exposures, and (vi) eosinophils are critical for recovery from chronic pulmonary inflammation and dysfunction after multiple parasite exposures in mice.
Evaluation of immunological and parasitological parameters in this study suggest a collaborative cross-talk of innate and adaptative immune responses during pulmonary larval ascariasis in mice. The association between eosinophils and SIgA creates a protective environment against larval migration, resulting in the control of lung damage and dysfunction due to the decrease in parasite burden. Eosinophil activation, degranulation, and cytokine production are mechanisms that may ascribe protection against ascariasis and other helminthiases. The role of eosinophils in helminth protection, as well as the interaction of SIgA and eosinophils in showing immunostained cells predominantly in the peribronchial spaces and the bronchial epithelium. Non-infected WT BALB/c (B), non-infected GATA1 -/-(C), single-infected WT BALB/c (D), single infected GATA1 -/-(E), re-infected WT BALB/c (F), and re-infected GATA1 -/-(G) (n = 6). IgA is marked in brown. Arrows indicate positive markings on the bronchial epithelium. Bar scale = 40 μm). https://doi.org/10.1371/journal.ppat.1010067.g008

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Eosinophils mediate ascariasis control

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Eosinophils mediate ascariasis control helminth protection, has been well documented [17,21,32]. SIgA interaction with eosinophils regulates both, eosinophil survival and gene expression [33]. SIgA binding alone can prime eosinophils and increase cytokine production and survival while binding of SIgA magnification (Bar scale = 50 μm) (E-H), showing histopathological lesions in the single-infected group of WT BALB/c (A and E) and GATA-/-(B and F) (n = 6), and re-infected WT BALB/c (C and G) and GATA1 -/groups (D and H) (n = 6). Thickened septa are indicated with arrowheads, inflammatory infiltrates are indicated with '#', hemorrhage is indicated with ' � '. Optical density represents eosinophil peroxidase (EPO) and neutrophil myeloperoxidase (MPO) activity in the lung tissue at the 8 th dpi (n = 10) (I). Tissue cytokine profile in the 8 th dpi (n = 10) (J). Differences between groups were statistically evaluated using one-way ANOVA followed by Tukey´s multiple comparisons test for the data represented in (I) and the Kruskal-Wallis followed by Dunn's multiple comparisons test in the data represented in (J). Data are represented as mean ± SEM. P values are represented by symbols in the graphs wherein � represents differences between non-infected groups, � represents differences between single-infected groups, # represents differences between groups from different strains that received the same treatment. The area of fibrosis is represented by ' � '. Bar graphs from morphometric analysis of the area of the lesion (E) and fibrosis (F) caused by larval migration and lung inflammation (n = 6). Assessment of lung mechanics in mice after single and multiple infections with A. suum (G) (n = 6). Data are represented as mean ± SEM. One-way ANOVA followed by Tukey´s multiple comparisons test was used to evaluate differences between groups in (E) and (F). Kruskal-Wallis test followed by Dunn´s multiple comparisons test was used to evaluate differences among groups in (G). Statistical differences are represented by symbols in the graphs, where � represents differences observed between non-infected groups, � represents differences between single-infected groups, # represents differences between groups of different strains that received the same treatment. [

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Eosinophils mediate ascariasis control immunocomplexes with antigens can completely activate the degranulation and superoxide release effector functions of eosinophils [19], as in the case of IgG binding [34,35]. In addition, it has been reported that eosinophils are important sources of APRIL and IL-6 mediators, which are crucial for plasma cell activation and survival, and the absence of eosinophils, the plasma cells quickly die of apoptosis [20,33]. A study in eosinophil-deficient mice revealed that eosinophils also contribute to maintaining plasma cell survival in both Th1 and Th2 immune responses in mice [19,20].
In addition to eosinophils, other immunological cells such as lymphocytes and macrophages, especially those alternatively activated (M2), are important for protection against helminths, repair of damaged tissues, and immune response modulation during helminth infection [36][37][38]. Furthermore, helper T cells and macrophages, or factors derived from such cells, participate in the induction of IgA production [39,40]. Corroborating these findings, our data indicated that, in addition to eosinophils, macrophages and lymphocytes may also participate in protection by promoting the production of IgA. However, elucidation of the role of lymphocytes and macrophages was not the focus of this study.
Several protective mechanisms associated with SIgA have been described related to its protective function against helminths. These include potent eosinophil degranulation, helminth larvae entrapment, phagocytosis mediated by alternative complement cascade activation, and neutralization of proteins secreted/excreted by the parasite [7]. Although recent studies have shown that eosinophils are not necessary to maintain IgA levels in the absence of infection [41][42][43], eosinophil-deficient mouse models show a reduction in the number of plasma cells, IgA and SIgA levels in the intestinal lamina propria [32,[44][45][46].
It is hypothesized that the presence of innate SIgA at the site of infection is a natural protective factor that hampers larval migration. In our study, the increase in specific and total SIgA concentrations associated with negative correlations between SIgA concentration and parasite burden supports the hypothesis that SIgA plays a protective role in ascariasis and mitigating the damage caused by larval migration. This was established using GATA1 -/mice that showed a significant reduction in SIgA levels in BAL and lung parenchyma and increased parasite burden with consequent pulmonary dysfunction.
Total SIgA production and the presence of leukocytes in the BAL are dependent on the time of infection. Moreover, positive correlations between parasite burden and immunological variables are consistent with BALT biology because, in mice and humans, BALT is only induced by infections [9]. Corroborating these results, we found positive correlations between SIgA in the BAL and markers of preserved pulmonary function, such as dynamic compliance and pulmonary resistance. Thus, as the larvae migrate to the pulmonary parenchyma, BALT formation and consequent cellular and humoral immune responses occur. Higher SIgA levels in the BAL may reduce parasite burden and consequently, the pathologies associated with pulmonary parenchymal migration and tissue remodeling.
Acute inflammation releases numerous mediators and cytokines that result in cell infiltration and subepithelial fibrosis, which are immunopathological processes associated with larval migration in ascariasis [3,32,[44][45][46]. Ascariasis is characterized by diffuse chronic inflammation of the pulmonary parenchyma with an increase in eosinophils, neutrophils, macrophages, and lymphocytes in the parenchyma and airways, highlighting the mixed character of inflammation and pulmonary pathology typically observed in this disease. In addition, the presence of eosinophils after multiple exposures is critical to the pathogenesis of larval ascariasis, as eosinophils regulate and promote the maintenance of alternatively activated macrophages [47]. Thus, they contribute to the expulsion of parasites and promote type 2 pathogenic responses, as well as tissue repair [48]. Tissue remodeling improved respiratory capacity in reinfected WT BALB/c mice, even with increased tissue fibrosis, compared to re-infected GATA1 -/mice, with a higher parasite burden. This was probably due to the severity of tissue damage observed in mice due to a large number of larvae migrating through the organs. In this regard, our results improve our understanding of the role of eosinophils and SIgA in larval ascariasis, although more studies are needed to elucidate the mechanisms involved in the development of resistance to primary infection and re-infection.
Finally, the greater susceptibility to A. suum infection in TLR2-and TLR4-deficient mice was associated with a decrease in eosinophils and production of total and antigen-specific SIgA antibodies in the pulmonary mucosa. This suggests that the increase in eosinophils is dependent on TLR2 and TLR4 sensing and eosinophils modulate SIgA levels, mainly in reinfected animals. Besides, similar results have been obtained with different parasites; for example, a study with Taenia crassiceps showed that TLR2 appears is essential for limiting infection during experimental cysticercosis, as TLR2 signaling pathways are involved in the recognition and subsequent activation of the innate immune system and production of inflammatory cytokines [49]. Experimental infections with Schistosoma sp. showed that TLR2 is involved in modulating the immune response against soluble egg antigen [50,51], which reduces immunopathology, despite favoring the persistence of infection. Moreover, experimental infections revealed the role of TLR4 in protective immunity against larvae of Onchocerca volvulus [52] and Strongyloides stercoralis [53].
TLR is known to be expressed on the cell surface of a large variety of cells such as dendritic cells, macrophages, B lymphocytes, eosinophils, and lung epithelium cells [54]. Helminths have a wide variety of glycoproteins and glycolipids that bind to TLRs on the host cell surface and activate or modulate the immune response mediated by such receptors [55]. It was demonstrated that Taenia crassiceps carbohydrates (TCHO) having repetitive structure characteristic of PAMPs, can link to TLR4 and, to a lesser extent, TLR2; the interaction of TCHO with TLR4 induces IL-6 expression in naïve murine macrophages [49]. A similar mechanism may occur in eosinophils triggered by helminth molecules, probably inducing IL-5 expression, a pleiotropic cytokine that can function as a growth factor for the B-1 cell subset associated with autoantibody production [56], as well as induce B cells to switch to IgA [57], and B cell IgA + differentiation [58].
Proteomic evaluation of hepatic resistance to larval ascariasis revealed upregulation of genes related to innate and adaptive immune responses in Ascaris-infected mice [59]. These genes may act as markers of danger-associated molecular patterns (DAMPs) interacting with TLR4 like that observed in asthma [60,61]. Increased TLR2 and TLR4 expression in circulating B-cells during helminth infection has already been demonstrated, reflecting systemic exposure to the microbial ligands [62]. Therefore, a possible mechanism linking TLR activation with SIgA production has already been described [54]. A third signal for efficient naïve B cell proliferation, isotypic exchange for IgG and IgA, and differentiation of antibody-secreting cells has also been shown. A direct signal may be delivered by direct TLR activation in the endosomal compartment or on the plasma membrane of B cells. In addition, the interaction of cytokines produced by TLR-activated dendritic cells may be an indirect signal [63]. TLR4 activation, as well as the presence of tissue damage, induces a considerable increase in polyreactive antibodies [64][65][66]. Furthermore, DAMP signaling induces somatic hypermutation and leads to an increased antigenic affinity for polyreactive antibodies [67,68]. This process seems to be due to the ambivalent action of this immunoglobulin, as it allows SIgA to discriminate and act against potentially harmful pathogens while acting as an anti-inflammatory molecule in the mucosal homeostasis process [69].
With our growing understanding of intestinal mucosa homeostasis, the existence of an intricate network between microbiota composition, development of immunity, and the mechanisms of susceptibility/resistance to helminth infections is becoming more evident. The presence of helminths in the intestinal mucosa may modify the composition and abundance of bacterial species that make up the intestinal microbiota. Similarly, variations in microbiota composition may alter the susceptibility of the host. This study showed that one of the resistance mechanisms against ascariasis is dependent on TLR2 and TLR4 signaling and the presence of eosinophils, which results in SIgA production.
The role of probiotics in modulating the immune response has been widely discussed. A genomic study associated with in vitro and in vivo experiments demonstrated that the use of the probiotic Lactobacillus delbrueckii subsp. lactis (CIDCA 133) induced increased expression of TLR2, TLR4, and MyD88 genes, with subsequent production of cytokines [70]. Similar results were found with the use of the probiotics Lacticaseibacillus casei and Saccharomyces boulardii [71,72].
Oral administration of the probiotic Lactobacillus gasseri (SBT 2055) in mice caused an increase in IgA levels and the rate of IgA + cell populations in Peyer's patches and the lamina propria of the small intestine, as well as in cultures of B cells and dendritic cells derived from bone marrow (BMDC). The addition of anti-TLR2 antibodies inhibits probiotic-induced IgA production [73].
We demonstrated that a diet containing probiotic Lactococcus induced a considerable increase in the number of eosinophils in the pulmonary mucosa, as well as the production of total SIgA, specific parasitic immunoglobulin, and an absolute number of cell subpopulations in the BAL. These findings suggest that the probiotic bacteria L. lactis (NCDO 2118) acts as an adjuvant in inducing a mucosal immune response against A. suum larvae.
Our findings corroborate the data by FitzPatrick et al. (2020), who described high levels of total SIgA under natural conditions and without infection in the absence of eosinophils [41]. However, we also found that eosinophils are essential for the maintenance of high levels of this immunoglobulin after infection with A. suum, corroborating the findings of previous studies [32,[44][45][46]. We also noticed high levels of antibodies after infection in GATA1 -/mice, which may have contributed to the reduced parasite burden in these animals.
Although Luerce et al. (2014) demonstrated that L. lactis (NCDO 2118) did not alter SIgA production under physiological conditions in WT C57BL/6 mice (2, 3, and 4 days after treatment) [22], our results showed that after 22 days of treatment, there was a significant increase in SIgA in BALB/c and GATA1 -/mice. These findings are supported by previous studies that demonstrated that during intestinal parasitic infections, additional eosinophils are recruited into the intestinal tract [74,75]. Moreover, a local eosinophilic response may also induce an increased number of eosinophils in remote sites [76]. Although some variations in basal SIgA levels have been observed in non-infected mice before, the literature suggests that variations in the native microbiota are natural [41]. It has also been reported that WT BALB/c and WT C57BL/6 mice strains differ in polyreactive IgA abundance and specificity, as well as in microbiota diversity [77].
Innate and adaptive immune responses are intrinsically connected, and their cross-talk is very important for homeostasis and is crucial in dealing with helminthiasis. Taken together, our results highlight the importance of eosinophils in mediating IgA production in mucosal sites, tissue inflammation, and remodeling, as well as controlling parasite burden during experimental larval ascariasis in mice. In conclusion, this study reinforces the known essential nature of eosinophils by coordinating multiple factors involved in the immune response to helminth infections. In addition, we postulate that stimulation of TLR may contribute to controlling parasitic burden via eosinophils and SIgA.

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Eosinophils mediate ascariasis control RT-qPCR data were obtained using the cDNA of non-infected (NI) and single-infected (SI) BALB/c and C57BL/6 mice and reinfected (RE) BALB/c mice (n = 5 / group). The relative expression of TLR-2 and TLR-4 mRNA was normalized to the reference gene β-actin and the fold change was calculated using the 2 (-ΔΔCt) method. The fold change was expressed as mean ± standard deviation for all groups. The Mann-Whitney test was used to evaluate the differences between the groups (A and D). One-way ANOVA followed by Tukey´s multiple comparisons test was used to evaluate the differences between groups (B and E). Statistical differences are represented by symbols in the graphs, where � represents p < 0.05, �� represents p < 0.01. All experiments were performed on the 8th dpi. RT-qPCR products were analyzed using 2% agarose gel electrophoresis. The 191 bp and 249 bp bands amplicons correspond to the amplified fragments of the TLR-2 (C) and TLR-4 (F) cDNA, respectively. bp: base pairs. (TIF)

S4 Fig. Total
SIgA and antigen-specific antibodies IgM, IgG1, IgG2A, IgG2B, and IgG3 were measured by ELISA in WT BALB/c and GATA1-/-mice after single-and re-infection. P values are represented by symbols in the graphs wherein � represents differences between the non-infected groups of the same strain, � represents differences between the single-infected groups of the same strain, # represents differences between groups from different strains that received the same treatment. One-way ANOVA followed by Tukey´s multiple comparisons test was used to evaluate differences between groups (A-F).