Aspartyl Proteinases of Eukaryotic Microbial Pathogens: From Eating to Heating

1 Polo d’innovazione della Genomica, Genetica e Biologia, University of Perugia, Perugia, Italy, 2 Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy, 3 Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology—Hans Knoell Institute, Jena, Germany, 4 Center for Sepsis Control and Care, University Hospital Jena, Jena, Germany, 5 Friedrich-Schiller-Universität, Jena, Germany


Plasmepsins
The AP "eating" functions and their consequences for the pathogen-host relationship are well represented by plasmepsins of P. falciparum. This is a major agent of malaria, a disease with an estimated more than 200 million cases and 438,000 deaths in 2015 [10]. Of the ten P. falciparum plasmepsins, I-IV are involved in hydrolyzing host hemoglobin and removal of its toxic products, acting together with other non-aspartyl proteinases such as the falcipains and falcilysins within the Haem Degradation Protein (HDP) complex at the intra-erythrocytic stage [8,9]. This process enables the parasite to utilize hemoglobin as an amino acid source and avoid potential damage by iron and haem molecules through the formation of hemozoin crystals. On the other hand, plasmepsin V is involved in the export of malarial effector proteins through the endoplasmic reticulum to the erythrocyte, a crucial activity for protozoan survival [11][12]. In fact, plasmepsin V cleaves a factor named Plasmodial Export Element (PEXEL) that allows for the export of malarial proteins into the host cell. Some of the exported proteins play an important role in virulence and antigen presentation [11][12][13][14][15][16][17][18]. A similar role appears to be played by ASP5, a phylogenetically related AP of Toxoplasma gondii, the deletion of which makes T. gondii unable to cleave the PEXEL-like motif, thereby negatively impacting parasite fitness and virulence in vivo [19][20][21]. The other five plasmepsins in P. falciparum are not components of the food vacuole HDP, and their functions are poorly understood. However, plasmepsins VII and X have recently been detected in ookinetes and zygotes of P. falciparum, and Left. The molecular ribbon-like structure of Sap2, a major AP of Candida albicans. Note the flexible flaps that control the access to the central region faced and delimited by the two active sites DTGS and DSGT and accommodating an enzyme inhibitor. N-ter is the N-terminus and C-ter the C-terminus of the amino-acid sequence. Right. Sequences of Sap2 and plasmepsin II of Plasmodium falciparum, which is most similar to Sap2 among the APs of eukaryotic microbial pathogens, showing two regions of high similarity (highlighted in red). The identity of the two whole sequences is 28.2% and their similarity 57.4% (FASTA; MBL Swiss-Prot). antibodies against these plasmepsins have been shown to reduce the infectivity of P. falciparum for mosquitoes [22]. For all the above reasons, plasmepsins have become attractive targets for possible chemo-and immuno-therapeutic interventions. In particular, the virulence-attenuated, plasmepsin IV-deleted strain of P. berghei, an agent of murine malaria, has been suggested as a blood-stage, whole-cell vaccine [23][24]. The plasmepsins' redundancy and consequent stringent requirements for their use of molecular targets of new plasmodial inhibitors has been critically discussed [9].

Secretory Aspartyl Proteinases
Several APs are produced, often in a secretory form, by many fungal organisms, including both primary and opportunistic pathogens. For example, a glycosylated AP from Paracoccidioides brasiliensis, the agent of a deep-seated mycosis in Latin America, and a non-glycosylated AP from Coccidioides posadasai, an agent of coccidioidomycosis in America, have been isolated and characterized [25,26]. For this latter, highly pathogenic fungus, a recombinant AP was generated and shown to induce a protective immune response against a pulmonary infection in mice [26]. However, the role of APs in pathogenesis and immune responses has been most extensively investigated in the opportunistic fungus C. albicans, providing evidence for previously unsuspected AP "heating" functions. These functions should be considered an important, in some pathologies a likely dominant, addition to the AP broad spectrum of hostimpacting, protein degradation activities.
C. albicans is a eukaryotic pathogen able to thrive equally well as commensal or as pathogen in humans [27]. The fungus can cause lethal systemic infections in immunocompromised or severely debilitated subjects but more frequently cause superficial infections, some of which (for instance, the recurrent vulvovaginal infection [RVVC]) are regularly observed in women without any apparent immune deficit [28]. As detailed below, there is good evidence that the expansion of C. albicans SAP genes contribute significantly to the virulence program of this fungus in the vaginal disease.
Of the ten C. albicans Saps, eight are secreted into the extracellular space (Sap1-8), and two are cell membrane-or cell wall-associated (Sap9-10). They have been reported to play different, although redundant, pathogenic roles, in part associated with the characteristic yeast-tohypha transition, which is central in the biology and pathogenicity of this fungus [7,29,30] (Fig 2).

A Blend of Immunoevasion and Immunoactivation
Candidal vaginitis is a disease in which Sap activities seem to have a major impact on host immunity. In fact, in vitro, ex vivo, and animal investigations in distant and immunologically different rodent models (rat and mouse) matched some old clinical data in support of a role for Saps in determining or co-determining the disease [31,32]. However, the mechanisms by which Saps contribute to disease have long remained uncertain, sometimes blurred by the simultaneous expression or activity of other numerous putative virulence traits expressed by this organism [29]. Hypothetical mechanisms mostly focused on Sap capacity to hydrolyze structural proteins of epithelial cells (e.g., E-cadherin) or factors of both innate and adaptive immunity, particularly complement, that allow C. albicans to prevent or escape from local host immunity and damaging epithelial cells [33-36].
More recent data suggest a perhaps more relevant role of Saps in RVVC, i.e., inducing pathogenic inflammation at an inflammation-non-permitted, tolerant body site. It has been demonstrated that some Saps are pro-inflammatory proteins capable of inducing a potent damage response through endocellular inflammasome receptors, particularly the NLRP3 inflammasome, in both hemopoietic and epithelial tissues [37][38][39]. While adding to the list of other identified or supposed fungal inflammasome activators [40], Saps are the first identified fungal inducers of inflammasome activation of a pure protein nature. Importantly, Sapinduced pro-inflammatory activity, unlike all other Sap functions, does not rely on enzymatic activity [37]. In mouse models of vaginal infection, some Saps (mostly Sap2 and Sap6, or Sap5, depending on the model [33,39]), appear to be responsible for the expression of key immunopathogenic markers of landmark inflammatory events. This includes polymorphonuclear (PMN) cell infiltration and production of pro-inflammatory cytokines, such as IL-1β and IL-18, via the activation of inflammasome-mediated caspase-1 and possibly other caspases [41]. The use of individual or collective subfamily SAP knockout strains, human anti-Sap Fragment antigen binding (Fab), and whole transcriptome analyses has shown that inflammation in vaginal disease can be dampened either downstream, by pharmacological inhibition of NLRP3 inflammasome and blockade of IL-1β receptor, or upstream, by specifically affecting Sap production or activity by anti-Sap antibodies or pepstatin A, without affecting the infectious fungus burden [38,39] (Fig 3).
This latter observation suggests that the protective capacity of a recombinant Sap2 vaccine [42] could be mediated by anti-inflammatory antibodies. Interestingly, both NLRP3 and NLRC4 inflammasomes were activated during C. albicans infection, and NLRC4 and IL-22 were shown to counteract the pathogenic inflammation sustained by NLRP3 [43]. These The SAP family of C. albicans contains at least ten proteins with a signal peptide and are secreted, except Sap9 and Sap10, which remain bound to the cell wall. They are characterized by broad spectrum proteolytic ability and virulence properties that are reported to be differentially expressed at different stages and forms of fungus growth and disease. Sap2 (alike Sap1 and Sap3) is active at acidic pH and is dominantly associated with yeast form of growth while Sap6 (alike Sap4 and Sap5) is more active at neutral to slightly alkaline pH Together with the dominant Sap5, Sap6 has been associated with hyphal growth. For details, see [7]  findings contrast the protective role of NLRP3 inflammasome reported for oral infections [44], further supporting the view that pathogenic and immune mechanisms vary significantly between vaginal and oral candidiasis [45].

Is C. albicans SAP-Induced Inflammation Shared by Other AP-Possessing Eukaryotic Pathogens?
Inflammasome activation and inflammatory cytokine cascades are associated with the pathogenesis of a number of diseases caused by other AP-possessing eukaryotic pathogens. Do these APs directly or indirectly participate in inflammation? As an example, do some plasmepsins participate in the inflammation typical of cerebral malaria (CM)? This disease is the worst outcome of infection with P. falciparum, being lethal or causing severe cognitive deficits in cured patients. Plasmepsin II is actively produced at the disease-critical blood stage of infection. As recently highlighted [46], upon infection with P. falciparum, the host immune system produces pro-inflammatory cytokines, including IL-1β, which activates endothelial cells that in turn produce CXCL10, a chemo-attractant for mononuclear leukocytes. Very little is known about the specific components of P. falciparum and other eukaryotic pathogens capable of Sap2 and Sap6 activate the NLRP3 inflammasome pathway through an early cascade of events, causing upstream NLRP3 inflammasome activation and downstream caspase-1-mediated cytokine production. Late events depend on Sap endocytosis inducing the translocation of NF-κB (p50/p65) into the nucleus, pro-IL-1β and pro-IL-18 synthesis, then (through type I IFN production) caspase-11 activation that cooperates with the NLRP3 inflammasome in triggering downstream caspase-1-mediated cytokine production. For details about this proposed scheme of Sap/inflammasome/caspases activation, see [38], [39], and [42]. stimulating the inflammatory cascade. However, plasmepsin 4-deficient P. berghei do not cause CM in a model of murine malaria [47]. In light of what has been found in C. albicans, studies on the possible role of other eukaryotic APs in inflammasome activation are worthy of being considered.