Inhibition of Nuclear Transport of NF-ĸB p65 by the Salmonella Type III Secretion System Effector SpvD

Salmonella enterica replicates in macrophages through the action of effector proteins translocated across the vacuolar membrane by a type III secretion system (T3SS). Here we show that the SPI-2 T3SS effector SpvD suppresses proinflammatory immune responses. SpvD prevented activation of an NF-ĸB-dependent promoter and caused nuclear accumulation of importin-α, which is required for nuclear import of p65. SpvD interacted specifically with the exportin Xpo2, which mediates nuclear-cytoplasmic recycling of importins. We propose that interaction between SpvD and Xpo2 disrupts the normal recycling of importin-α from the nucleus, leading to a defect in nuclear translocation of p65 and inhibition of activation of NF-ĸB regulated promoters. SpvD down-regulated pro-inflammatory responses and contributed to systemic growth of bacteria in mice. This work shows that a bacterial pathogen can manipulate host cell immune responses by interfering with the nuclear transport machinery.


Introduction
Many of these are likely to be due to the predominant effect of LPS on pro-inflammatory signalling pathways, since LPS and Salmonella cells induced similar changes in RAW 264.7 macrophages gene expression at 4 h post-challenge [23]. To analyse the effect of the SPI-2 T3SS in the context of TLR4-induced responses, we selected three genes (tnf-α, il1-β and serpinB2) that are induced by LPS [23,24]. Their mRNA levels were measured in BMM from wild-type and TLR4 knock-out (TLR4 -/-) mice, infected with wild-type or ΔssaV (SPI-2 T3SS null mutant) S. Typhimurium for 10 h to allow sufficient time for SPI-2 T3SS effector translocation and activity, while minimising the difference in overall intracellular growth rate between wild-type and ΔssaV mutant bacteria. The fold-increase in intracellular bacterial numbers (calculated from the ratio of the cfu at 10 h compared to 2 h post-uptake) was 2.20 ± 0.34 for the wild-type and 0.89 ± 0.09 for ΔssaV mutant bacteria. Following infection of wild-type BMM for 10 h, the RNA levels of tnf-α, il1-β and serpinB2 (quantified by real-time PCR (qRT-PCR)) increased by between 40 and 400 fold, and no significant differences were observed between BMM infected with wild-type or ΔssaV mutant bacteria (Fig 1A and 1B, S1 Fig). Similar increases were detected following exposure of wild-type BMM to LPS, suggesting that TLR4 signalling accounted for the majority of these changes (Fig 1A and 1B, S1 Fig). However, TLR4 -/-BMM infected with the ΔssaV mutant had statistically significant higher levels of tnf-α and il1-β mRNA transcripts than BMM infected with wild-type bacteria (Fig 1A and 1B). No difference was observed for serpinB2 mRNA transcripts levels (S1 Fig), indicating that transcription of not all the LPS-responsive genes was affected by the SPI2-T3SS. Levels of secreted TNF-α from TLR4 -/-BMM infected with SPI-2 null mutants (ΔssaV or ΔsseB) were also significantly greater than from TLR4 -/-BMM infected with wild-type bacteria or a complemented SPI-2 null mutant (ΔsseB, psseB) ( Fig 1C). The level of secreted Il1-β was below the threshold of detection at 10 h post-bacterial uptake but was also reduced in a SPI-2 T3SS-dependent manner at 24 h postbacterial uptake (Fig 1D). Together these results provide evidence that the SPI-2 T3SS suppresses pro-inflammatory cytokine mRNA levels and protein secretion in TLR4 -/macrophages. The failure to detect such differences using wild-type BMM presumably reflects the potent effect of LPS on pro-inflammatory signalling pathways in this experimental system.

SpvD is a SPI-2 T3SS effector suppressing cytokine secretion
To identify SPI-2 T3SS effector(s) involved in down-regulation of pro-inflammatory immune responses, several SPI-2 T3SS mutant strains (representing single mutants lacking individual effectors) [25] were screened for levels of secreted TNF-α from TLR4 -/-BMM at 10 h post-uptake (S1 Table). Of these, 2 strains (ΔspvC and ΔspvD) induced significantly more cytokine secretion from infected BMM compared to BMM infected by the wild-type strain (Fig 1E and 1F). SpvC and SpvD are encoded by the spvRD operon (composed of spvA, B, C and D, and regulated by spvR [26] on the Salmonella virulence plasmid (pSLT)) and SpvC is a phosphothreonine lyase that interferes with MAPK signalling [14]. BMM infected with deletion mutants carrying the low copy number plasmid pACYC184 containing the corresponding wild-type allele of the gene (ΔspvC, pspvC or ΔspvD, pspvD) produced significantly less TNF-α and Il1-β than BMM infected with the single mutant strains (Fig 1E and 1F). To analyse if the effects of SpvD are confined to macrophages, Hela cells were infected with Salmonella strains for 8, 12 or 24 h and levels of IL-8 in supernatants were quantified by ELISA. Infection with wild-type bacteria induced IL-8 production but no increased production was detected in cells infected with the ΔspvD mutant or the ΔspvC mutant (as shown in [14]) (S2 Fig). However, cells infected with the ΔspvD, pspvD or ΔspvC, pspvC strains showed significantly less IL-8 production compared with the wild-type strain at all time-points tested (P 0.05 for all time points). Therefore, the effects of SpvD occur both in macrophages and another cell type that does not normally respond to LPS. Wild-type and TLR4 -/-BMMs were non-infected (NI), exposed to LPS (100 ng/ml) or infected with wild-type or ΔssaV strains for 10 h and mRNA levels of tnf-α (A) and il-1β (B) were analysed. The transcript levels were normalized to the levels of rsp9, which were constant under all conditions used, and then expressed relative to those of NI wild-type BMMs. Results are expressed as mean ± SEM of at least 3 independent experiments. Levels of secreted TNF-α (C) and Il-1β (D) were quantified by ELISA in supernatants of non-infected (NI) TLR4 -/-BMMs, TLR4 -/-BMMs stimulated with LPS or TLR4 -/-BMMs infected with indicated strains of S. Typhimurium at 10 h or 24 h post-uptake respectively. Results presented are the means ± SEM of triplicates of one experiment. These results are representative of three independent experiments and significant differences were observed in each experiment. P-values were obtained using two-tailed unpaired Student's t-test (*p < 0.05). Levels of secreted TNF-α at 10 h post-uptake (E) and Il-1β at 24 h post-uptake (F) were quantified by ELISA in supernatants of TLR4 -/-BMMs infected with indicated strains of S. Typhimurium. The cytokine levels were expressed relative to those of BMMs infected with wild-type bacteria. Results are expressed as SPI-2 T3SS-dependent secretion of SpvD into minimal medium and its translocation into host cells (using a CyaA fusion protein) were shown previously [29]; however its function is unknown and its contribution to virulence of S. Typhimurium in mice is not clear [27][28][29][30]. To confirm SPI-2 T3SS-dependent secretion and translocation of SpvD, strains producing double-HA tagged SpvD from pSLT were used for an in vitro secretion assay and for macrophage infection. Secretion of SpvD-2HA from bacteria grown in minimal medium was investigated following shift of ambient pH from 5.0 to 7.2, which stimulates effector secretion from the SPI-2 T3SS [31]. A similar amount of SpvD-2HA was present in lysates from wild-type and ΔssaV mutant bacteria (S3A Fig). SpvD-2HA was also detected in the secreted fraction from wildtype bacteria but not from the ΔssaV mutant. To investigate SpvD translocation into host cells, RAW macrophages were infected for 22 h with Salmonella strains producing SpvD-2HA. Cells were then fixed and labelled to detect Salmonella and the HA tag. Translocated SpvD-2HA was not detected within infected macrophages by immunofluorescence microscopy under these conditions. However when cells were incubated for 2 h prior to fixation with the proteasome inhibitor MG132, weak but distinct and reproducible cytoplasmic labelling of SpvD-2HA was detected in the cytoplasm of the majority of cells infected with wild-type but not ΔssaV mutant Salmonella (S3B Fig). These results suggest that relatively small amounts of SpvD are translocated into the host cell cytoplasm.
Since SpvC inhibits MAP kinase signalling [14] we tested if SpvD affects this pathway in cells expressing a luciferase reporter gene under the control of MAPK-dependent AP-1 binding sequences. HEK 293 cells were co-transfected with vectors expressing myc-SpvD or myc-SpvC (as positive control) or myc vector alone (empty pRK5 vector), along with vector expressing the luciferase reporter under the control of AP-1 promoter and vector constitutively expressing the Renilla luciferase, as an internal control for normalisation of data. Cells were exposed to PMA for 6 h to stimulate MAP kinase pathways. Then cells were lysed and bioluminescence was measured to determine the fold-difference of MAP kinase activation in relation to nontransfected cells. Immunoblots of cell lysates following SDS-PAGE, using anti-myc and antitubulin antibodies, confirmed that similar numbers of cells expressing similar levels of effectors were analysed. Ectopic expression of SpvC resulted in a strong inhibition of luciferase activity, while transfection of empty vector or of vector producing SpvD did not inhibit luciferase activity (Fig 2A), suggesting that unlike SpvC, SpvD does not inhibit the MAP kinase signalling pathway.

SpvD prevents nuclear accumulation of p65
Some T3SS effectors inhibit pro-inflammatory cytokine production and secretion by interfering with proteins of the NF-κB pathway [6]. To determine the potential effect of SpvD on nuclear translocation of the NF-κB transcription factor p65, confocal microscopy was used to quantify the amounts of nuclear p65 in TLR4 -/-BMM infected with different Salmonella strains at 10 h post-bacterial uptake. Cells were immunolabelled with anti-Salmonella and anti-p65 antibodies and nuclei were stained with the DNA dye, DRAQ5 (Fig 2B). Three-dimensional image projections were acquired randomly and nuclear p65 intensity was quantified using Volocity software. The intensity of p65 in the nuclei of infected TLR4 -/-BMM was significantly increased when SpvD was absent (ΔspvD, ΔspvRD (corresponding to ΔspvR-spvA-spvB-spvC-spvD mutant strain) or ΔspvRD,pspvC strains) and was restored to wild-type levels by mutants carrying pspvD (ΔspvD,pspvD or ΔspvRD,pspvD strains) (Fig 2C), indicating that of spv-mean ± SEM of at least 3 independent experiments. Statistical significances were calculated using ANOVA followed by Bonferonni's multiple comparison test against wild-type strain.
doi:10.1371/journal.ppat.1005653.g001 SpvD prevents nuclear accumulation of p65 but not degradation of IκBα. (A) SpvD does not inhibit activation of an AP-1-regulated promoter. HEK-293 cells were cotransfected with an AP-1-dependent luciferase reporter plasmid, pTK-Renilla luciferase and pRK5myc, pRK5myc-SpvC or pRK5myc-SpvD. Cells were then stimulated with PMA for 6 h. Firefly luciferase activity was normalized against Renilla luciferase activity. Results are expressed as fold induction compared to non-transfected (NT) cells. Values are expressed as mean ± SEM of 3 independent experiments and Pvalues were obtained using two-tailed unpaired Student's t-test (*** p < 0.005). (B-C) SpvD prevents nuclear translocation of p65. (B) Representative immunofluorescence fields of p65 localisation using anti-p65 (red) in TLR4 -/-BMMs infected for 10 h with indicated Salmonella strains (blue). Cell nuclei were stained with DRAQ5 (green). Scale bar, 8 μm. (C) Quantification above background levels of p65 intensity in the nucleus was analyzed by three-dimensional (3D) confocal encoded proteins, only SpvD prevents nuclear accumulation of p65. NleC, a T3SS effector of enteropathogenic E. coli is a protease that cleaves p65 directly [32][33][34][35]. Therefore, we analysed total cell levels of p65 in TLR4 -/-BMM infected with different Salmonella strains at 10 h postuptake. No significant differences were detected (S4A Fig). To determine if the influence of SpvD on p65 localisation was cell-type specific, its effects on p65 and its localisation were analysed during infection of HeLa epithelial cells. The amount of p65 in total cell lysates and in nuclei of HeLa cells infected with Salmonella strains was quantified by immunoblot after cell fractionation. No differences were detected in total cell extracts, but the amount of p65 in the nuclei of cells infected with the ΔspvD mutant was consistently increased compared to wildtype infected cells (S4B Fig). The amount of nuclear p65 was restored to wild-type levels in cells infected with the ΔspvD mutant carrying a plasmid containing spvD (S4B Fig). In addition, total levels of p65 in HeLa cells transfected with pRK5myc-SpvD, pRK5myc-SpvC (used as a negative control) or pRK5myc-NleC (used as a positive control) were analysed by FACS after labelling of the cells with anti-p65 and anti-myc antibodies. Levels of p65 were determined in myc-positive cells and normalised to non-transfected cells. Degradation of p65 occurred only in cells expressing NleC (S4C and S4D Fig). These results indicate that SpvD reduces nuclear translocation of p65 in both mouse macrophages and human epithelial cells, but does not affect the overall cellular pool of p65. The amounts of two other immune related transcription factors (p50 and STAT2) in total cell lysates and in nuclei of HeLa cells infected with Salmonella strains were quantified by immunoblot after cell fractionation. No differences were detected in total cell extracts, but the amount of p50 in the nuclei of cells infected with the ΔspvD mutant was consistently increased compared to wild-type -infected cells (S4B Fig). The amount of nuclear p50 was restored to wild-type levels in cells infected with the ΔspvD mutant carrying a plasmid containing spvD. No differences in STAT2 levels in nuclei or total cell lysates were detected. These results indicate that SpvD specifically targets the import of NF-κB -associated proteins.
The predicted product of spvD is a hydrophilic protein of 216 amino acids [36] and visual alignment of a region of SpvD with several prokaryotic phosphothreonine lyases [37] revealed a region in SpvD (between amino acids 181 to 213) that is conserved among these enzymes (S4E Fig). This area contains an invariant Lys residue (K185) and mutation of this residue in SpvC results in a loss of phosphothreonine lyase activity [38]. As a further test of the effect of SpvD on the NF-κB signalling pathway, and to investigate the importance of K185, HEK 293 cells were co-transfected with a reporter plasmid encoding luciferase under the control of NF-κB promoter and a plasmid encoding either myc alone (empty pRK5 vector), myc-SpvC (as a negative control), myc-SpvD or myc-SpvD K185A (SpvD carrying a lysine-to-alanine substitution at residue 185). Luciferase activity was assayed 8 h after TNF-α stimulation and normalised to non-transfected and unstimulated cells. Non-transfected cells underwent an approximately 20-fold increase in activation following stimulation with TNF-α when microscopy. Background levels were determined using signals measured in uninfected cells. P-values were obtained using two-tailed unpaired Student's t-test (*p < 0.05; ** p < 0.01). (D) SpvD inhibits activation of an NF-κB -regulated promoter. HEK-293 cells were co-transfected with an NF-κB -dependent luciferase reporter plasmid, pTK-Renilla luciferase and pRK5myc, pRK5myc-SpvC, pRK5myc-SpvD or pRK5myc-SpvD K185A at the indicated amounts (in ng). The NF-κB pathway was activated with TNF-α and luciferase activity was measured after 8 h. Results are expressed as fold activation in relation to unstimulated and non-transfected (NT) cells. Values are expressed as mean ± SEM of at least 3 independent experiments and statistical significances were calculated using ANOVA followed by Bonferonni's multiple comparison test against NT cells (*p < 0.05; ** p < 0.01; *** p < 0.005). (E) Degradation of IκBα is not affected by SpvD. HEK-293 cells transfected by pRK5myc-SpvD or pRK5myc-YopP were prepared at the indicated times after TNF-α stimulation and analysed by SDS-PAGE and immunoblotting with anti-IκBα and anti-tubulin antibodies. Ratio of IκBα normalised to unstimulated cells is indicated below immunoblots.
doi:10.1371/journal.ppat.1005653.g002 compared to resting cells. Transfection of 500 ng of empty vector or vector expressing SpvC did not prevent NF-κB activation, whereas expression of SpvD or SpvD K185A reduced activation by approximately 65% (Fig 2D). Inhibition of NF-κB activation by SpvD was observed after transfection of 50 ng of DNA and increased with increasing amounts of DNA. Together, these results indicate that SpvD is sufficient to block the activation of an NF-κB -regulated promoter and that K185 is not required for SpvD activity.
To investigate if SpvD interferes with IκBα degradation induced upon stimulation with TNF-α, HEK 293 cells were transfected with plasmids encoding either myc alone (empty vector), myc-SpvD or myc-YopP (a T3SS effector of Yersinia enterolitica that inhibits IκBα degradation [39][40][41], used here as a positive control), then stimulated with TNF-α for 5, 10 or 20 min to induce IκBα degradation. Proteins in cell extracts were then analyzed by SDS-PAGE and immunoblotting. As expected, degradation of IκBα was inhibited in cells expressing myc-YopP ( Fig 2E). However, IκBα was degraded in cells transfected with the empty vector or with the plasmid encoding myc-SpvD ( Fig 2E). Because only approximately 60% of the cell population was efficiently transfected (as measured by microscopic examination), a potential effect of SpvD on IκBα degradation might be difficult to detect when analysing the whole cell population by immunoblotting. Therefore, IκBα degradation following TNF-α stimulation was also analysed by flow cytometry after labelling of the cells with anti-IκBα and anti-myc antibodies. Levels of IκBα were quantified in myc-positive cells and normalised to non-stimulated cells. TNF-α -induced IκBα degradation was only prevented in cells expressing YopP (S4F Fig), indicating that SpvD does not inhibit IκBα degradation and that it must prevent nuclear accumulation of p65 by interfering with the NF-κB pathway after IκBα degradation.

Effect of SpvD on importin-α
Translocation of p65 into the nucleus requires proteins of the karyopherin (KPNA) family [42,43], also called importin-α. To determine the potential effect of SpvD on KPNA1 and KPNA3, two importin-α proteins known to interact with p65 [43][44][45], confocal microscopy was used to analyse their localisation in HeLa cells. Since attempts to detect endogenous KPNA1 or KPNA3 using commercial antibodies failed, HeLa cells were co-transfected with plasmids encoding either KPNA1-FLAG or KPNA3-FLAG and plasmids encoding myc-SpvD or myc-SpvC (as a control). Cells were immunolabelled with anti-FLAG, anti-myc and antilamin antibodies. Consistent with the localisation of SpvD following its translocation from bacteria (S3B Fig), SpvD was found in the host cell cytoplasm after transfection ( Fig 3A). In addition to nuclei containing diffuse labelling of KPNA1-FLAG or KPNA3-FLAG, in some nuclei, the labelling was predominantly in the region of the nuclear envelope, where it colocalised partially with lamin ( Fig 3A). This lamina-associated labelling was observed in all transfected cell populations, but its frequency was enhanced 2.7-fold by KPNA1 (P<0.001) and 1.6-fold by KPNA3 (P<0.05) when SpvD was co-expressed ( Fig 3B). In addition, the enhanced frequency of lamina-associated labelling was not observed when SpvC was co-expressed ( Fig 3B), indicating that it is specific to SpvD. To determine if the reorganisation of KPNA1 induced by myc-SpvD was localised inside or outside the nucleus, differential membrane permeabilisation was done using either Triton X-100 (under conditions in which it permeabilises both the plasma and nuclear membranes) or Saponin (at a concentration where it permeabilises only the plasma membrane). SpvD-induced accumulation of KPNA1 at the nuclear envelope was only observed when cells were permeabilised with Triton X-100, indicating that this importin accumulates within the nucleus (Fig 3C).
To analyse the effect of SpvD on importin-α localisation during infection, HeLa cells transfected with plasmids encoding KPNA1-FLAG or KPNA3-FLAG were infected for 14 h with Cells infected with the deletion mutant carrying a plasmid-borne wild-type allele of spvD induced a greater proportion of these structures than the deletion mutant ( Fig 3E; p<0.01). These structures might be an artefact of overexpression of KPNA1 and KPNA3 but do suggest an SpvDdependent accumulation of KPNA1 and KPNA3 in the nucleus. To analyse the effect of SpvD on the localisation of endogenous KPNA1, the amount of endogenous KPNA1 in nuclei of HeLa cells infected with Salmonella strains was quantified by immunoblotting after cell fractionation. A significant decrease in the amount of endogenous KPNA1 in the nuclei of cells infected with the ΔspvD mutant was detected, compared to wild-type Salmonella-infected cells (Fig 3F and 3G). The amount of nuclear KPNA1 was restored to that of the wild-type level in cells infected with the ΔspvD mutant carrying a plasmid containing spvD (Fig 3F and 3G). Therefore, in addition to inhibiting nuclear accumulation of p65, SpvD also causes nuclear accumulation of KPNA1.

SpvD interacts with Xpo2
Recycling of KPNAs from the nucleus to the cytoplasm is mediated by Xpo2 (also named CAS or CSE1) [46]. To confirm that depletion of Xpo2 inhibits recycling of KPNAs, confocal microscopy was used to analyse the localisation of KPNA1-FLAG in HeLa cells non-treated cells or cells exposed to a scrambled siRNA. However, the nuclear KPNA1 that was observed in the absence of Xpo2 was not localised at the lamina, as was observed in the presence of SpvD.
Knock-down of Xpo2 in HeLa cells was reported to cause a decrease of p65 translocation after stimulation with TNF-α [47]. To determine the effect of inhibition of Xpo2 expression on p65 localisation in our experiments, confocal microscopy was used to analyse localisation of p65 in HeLa cells depleted of Xpo-2 and stimulated with TNF-α for 45 min. Cells were immunolabelled with anti-Xpo-2 and anti-p65 antibodies and the intensity of p65 within the nucleus was quantified using Volocity software. siRNA of Xpo2 in HeLa cells led to a decrease in levels of nuclear p65 after stimulation with TNF-α compared to control cells (non-treated or scrambled siRNA) (S6D and S6E Fig). Since cytoplasmic SpvD and inhibition of Xpo2 expression both led to nuclear accumulation of KPNA1 and reduced levels of nuclear p65, we hypothesized that SpvD might interact with Xpo2. To investigate this, HEK cells were co-transfected with a plasmid encoding Xpo2-FLAG and a plasmid encoding either myc-SpvD or myc-SpvC. Cells were lysed and proteins immunoprecipitated with anti-myc antibody-conjugated beads. Immunoblot analysis revealed that SpvC failed to interact with Xpo2 but that SpvD and Xpo2 formed a complex (Fig 4A). To determine if SpvD interacts with endogenous Xpo2 in infected Triton X-100 and labelled with anti-FLAG (red) and anti-Salmonella (blue) antibodies. Cell nuclei were stained with DRAQ5 (green). Scale bar, 10 μm. (E) Quantification of cells with nuclear lamina-associated KPNA1 after infection with Salmonella strains. Values are expressed as mean ± SEM of at least 4 independent experiments (** p < 0.01; *** p < 0.005). (F) HeLa cells were infected for 14 h with Salmonella strains. Nuclear and total cell extracts were analysed by SDS-PAGE and immunoblotting with anti-KPNA1, anti-histone H3 and anti-GAPDH antibodies. (G) Ratio of KPNA1 in the nucleus normalised to wild-type -infected cells. Values are expressed as mean ± SEM of 3 independent experiments. P-values were obtained using two-tailed unpaired Student's t-test (*p < 0.05).
doi:10.1371/journal.ppat.1005653.g003 cells, RAW macrophages and HeLa cells were infected with Salmonella expressing either SpvD-HA or two other effectors translocated by the SPI-2 T3SS (SpvC-HA or SseL-HA), followed by immunoprecipitation using anti-HA conjugated beads. Input and output samples were analysed by SDS-PAGE and immunoblotting with anti-Xpo2 and anti-HA antibodies. SpvD bound to endogenous Xpo2 following its translocation from intracellular bacteria ( Fig  4B and 4C). No binding was observed when cells were infected with Salmonella expressing SpvC-2HA or SseL-2HA despite the fact that these two effectors were evidently more abundant than SpvD-2HA (Fig 4B and 4C). Xpo1 and Xpo5 are additional members of the exportin family, have some structural similarities to Xpo2 and mediate the export of proteins with a nuclear export signal and of RNA respectively [48]. However, when membranes were probed with anti-Xpo1 and anti-Xpo5 antibodies, no interaction between SpvD-2HA and these proteins was detected (Fig 4C). To analyse if the interaction of Xpo2 with SpvD affects the cellular localisation of Xpo2, the amount of Xpo2 in total cell lysates and in cytoplasm of HeLa cells infected with Salmonella strains was quantified by immunoblotting after cell fractionation. No cytoplasmic increase of Xpo2 in wild-type -infected cells was detected (S7A Fig). In addition, HeLa cells were transfected with plasmids encoding myc-SpvD or myc-SpvC (as control) and the amount of Xpo2 in total cells lysates and in cytoplasm was quantified by immunoblotting after cell fractionation (S7B Fig). No cytoplasmic increase of Xpo2 in cells transfected with plasmid encoding myc-SpvD was detected, suggesting that SpvD does not sequester Xpo2 in the cytoplasm.
These results show that SpvD localises to the host cell cytoplasm, inhibits nuclear accumulation of p65, causes nuclear accumulation of KPNA1 and KPNA3, and binds specifically to Xpo2. To determine if nuclear lamina-associated KPNA1 induced by SpvD is dependent on Xpo2, confocal microscopy was used to analyse the localisation of KPNA1-FLAG in HeLa cells depleted of Xpo2 and then transfected with a plasmid encoding KPNA1-FLAG with or without a second plasmid encoding myc-SpvD. Cells were immunolabelled with anti-FLAG and antimyc antibodies and the number of cells with nuclear lamina-associated KPNA1-FLAG were counted (Fig 4D). When SpvD was present, the frequency of lamina-associated KPNA1 was significantly enhanced by 2.1-fold in non-treated HeLa cells and by 2.0-fold in cells exposed to a scrambled siRNA. However, no statistical difference was observed in HeLa cells depleted of Xpo2. Together, these results indicate that the ability of SpvD to induce lamina-associated KPNA1 is dependent on the presence of Xpo2.

Effect of SpvD on virulence and cytokine expression in mice
Previous work failed to reveal a clear and consistent role of SpvD in virulence in vivo [27][28][29][30]. To reassess the possibility of an effect of SpvD on systemic growth of bacteria, mice were inoculated by intraperitoneal injection of a mixture of equivalent cfu of kanamycin resistant wildtype bacteria and the ΔspvD mutant strain. After 72 h of infection, bacteria were quantified after plating spleen homogenates on LB medium and LB medium containing kanamycin to distinguish the strains. The competitive index (CI) for the ΔspvD mutant was 0.48 ± 0.11 (P 0.05) when compared to wild-type (Fig 5A). To verify that this attenuation was due to loss of SpvD, a second experiment was conducted in which mice were infected as above but with a mixture of ΔspvD mutant and the same strain carrying spvD allele on the pACYC plasmid. The CI for this combination was 0.47 ± 0.11 (P 0.005) (Fig 5A), showing that the spvD allele with anti-myc and anti-FLAG antibodies. Intra-nuclear rings of KPNA1 were quantified blindly by microscopy. Values are expressed as mean ± SEM of 3 independent experiments and P-values were obtained using twotailed unpaired Student's t-test (*p < 0.05; ** p < 0.01).
doi:10.1371/journal.ppat.1005653.g004 rescues the growth defect of the mutant, thereby confirming that SpvD contributes to systemic virulence in the mice.
To assess the effect of SpvD on LPS-induced genes in mice, RNA levels of serpinB2, tnf-α and il1-β in infected splenic macrophages of C57BL/6 mice inoculated with Salmonella strains were compared by qRT-PCR (Fig 5B-5D). For serpinB2 mRNA transcripts, no significant differences were detected between mice infected with different Salmonella strains (Fig 5B). No significant differences in tnf-α and il1-β were detected between mice infected with the wild-type or ΔspvD mutant bacteria, but macrophages infected with ΔspvD, pspvD bacteria contained fewer tnf-α and il1-β transcripts (Fig 5C and 5D), indicating that SpvD can depress cytokine expression in vivo.

Discussion
As a basis for identifying SPI-2 T3SS effectors that interfere with host proinflammatory immune signalling, we analysed the effect of the SPI-2 T3SS on mRNA levels of tnf-α and il1-β in wild-type BMM at 10 h post-bacterial uptake. No obvious differences were detected, suggesting that either SPI-2 effectors do not have a significant effect on macrophage mRNA levels or that their activities are masked by the massive changes induced by LPS [23] and possibly other PAMPs. Use of TLR4 -/-BMMs provided evidence that the SPI-2 T3SS does suppress proinflammatory cytokine production. Among several SPI-2 T3SS effector mutants that were tested subsequently, infection with either ΔspvC or ΔspvD strains led to increased tnf-α and il1β secretion. SpvD and SpvC-dependent effects also occurred in HeLa cells, showing that their functions are not confined to macrophages. The effect of the ΔspvC mutant was unsurprising, since two previous studies have indicated an anti-inflammatory effect of SpvC, caused by its phosphothreonine lyase activity on ERK1/2, p38 and JNK [14,49].
SpvD was identified in a proteomic study as being secreted by both the SPI-1 and SPI-2 T3SSs and its translocation into J774 macrophage-like cells was confirmed using a CyaA fusion protein assay [29]. Using an epitope-tagged version under the control of its own promoter in the virulence plasmid, we showed that SpvD is secreted into the culture medium in a SPI-2 T3SS-dependent manner. SPI-2 T3SS-dependent translocation of SpvD into the cytoplasm of infected cells was also confirmed by immunofluorescence microscopy, but only when cells had been treated with a proteasome inhibitor. In addition, bacterially translocated SpvD-2HA was barely detectable in the input sample from infected HeLa cells and RAW macrophages, whereas SpvC-2HA and SseL-2HA were clearly more abundant (Fig 4B and 4C). This indicates that SpvD is translocated in relatively small amounts and/or is more prone to degradation compared to other effectors. Despite some amino acid similarity in a region containing the catalytic lysine of SpvC, SpvD did not require the corresponding lysine to suppress TNF-dependent activation of an NF-κB reporter. Furthermore, following transfection, SpvC but not SpvD inhibited activation of an AP-1-dependent promoter, showing that these effectors are likely to have distinct biochemical activities and physiological effects.
Several other bacterial effectors have been shown to interfere with NF-κB signalling. In most cases they affect the degradation of IκBα and/or the release of p65 [4][5][6]. For example, the Shigella flexneri kinase OspG interacts with host ubiquitin-conjugating enzymes to abrogate IκBα degradation and thus blocks nuclear translocation of p65 [50]. NleC of enteropathogenic and enterohaemorrahgic E. coli is a metalloprotease that degrades both free cytosolic p65 Results are expressed as mean ± SEM of at least 3 independent experiments. P-values were obtained using two-tailed unpaired Student's t-test (*p < 0.05). (E) Model depicting pathways targeted by SpvC and SpvD to overcome PAMP-induced defences in host cells. Immunoblots are representative of three independent experiments. doi:10.1371/journal.ppat.1005653.g005 and p65 when complexed with IκBα [33][34][35]. Abrogation of p65 translocation has also been described for the effectors NleB and NleE from enteropathogenic E. coli and OspZ from Shigella flexneri [51,52] NleE is an unusual methyltransferase that modifies crucial cysteines in the zinc finger domains in ubiquitin-chain sensory proteins TAB2 and TAB3 and thereby disrupts NF-κB signalling [53]. NleB is a N-acetylglucosamine transferase whose action blocks signalling from the TNFR and its associated adapters [54][55][56]. We found that SpvD prevents nuclear accumulation of p65 by interfering with the NF-κB pathway after IκBα degradation but it did not affect overall cellular levels of p65. SpvD might not be the only effector that inhibits the nuclear translocation of p65 following IκBα degradation. Indeed, the Bordetella effector BopN [57] localises to the nucleus and appears blocks nuclear translocation of p65 without affecting IκBα degradation; however, its host target(s) remain to be identified. SpvD might specifically target the import of NF-κB -associated proteins as it also prevents nuclear accumulation of NF-κB subunit p50 but not of STAT2.
There are 7 different importin-α (KPNA) isoforms and several have the capacity to interact with p65. Fagerlund et al. reported an interaction between p65 and both KPNA4 (α3) and KPNA3 (α4) but not KPNA2 (α1) [43,44] [45]. p65 might use different isoforms depending on host cell type, time and strength of pathway activation and other conditions. Furthermore, in vitro binding assays with purified proteins or lysates might not reflect normal physiological interactions. However, our results showing that SpvD affects nuclear accumulation of both p65 and p50 are consistent with its effect on KPNA4 (α3), which mediates nuclear import of both transcription factors [43].
We were unable to distinguish endogenous KPNAs by confocal microscopy using commercial antibodies and therefore cells were transfected with plasmids encoding FLAG-tagged KPNAs and anti-FLAG antibodies were used to detect them by confocal microscopy. Following transfection or bacterial translocation, SpvD stimulated a nuclear lamina-associated accumulation of overexpressed KPNA1 and KPNA3. No effect was observed on KPNA2 or KPNA4 localisation. We are unaware of other bacterial proteins that cause a similar effect, but Hantaan virus nucleocapsid protein N inhibits p65 nuclear translocation by binding directly to KPNAs [58]. We were unable to obtain evidence for an interaction between SpvD and KPNA1, 2, 3 or 4 by co-immunoprecipitation assays. Instead, following transfection or bacterial translocation, SpvD interacted specifically with endogenous Xpo2, which is required for recycling of KPNAs from the nucleus to the cytoplasm [46]. Inhibition of Xpo2 expression led to nuclear accumulation of KPNA1 and also reduced nuclear translocation of p65 ( [47] and this work). Despite apparently stable binding between SpvD and Xpo2, Xpo2 localisation was not noticeably affected by SpvD. It is possible that SpvD is an enzyme that modifies Xpo2. However, following immunoprecipitation by SpvD-2HA after infection of macrophages, mass spectrometric analysis of Xpo2 did not reveal any post-translational modifications such as phosphorylation, acetylation or conjugation by ubiquitin and ubiquitin-like proteins. It is possible that such modifications did occur but are relatively transient, labile or did not survive the extraction or ionization processes. Alternatively SpvD might affect Xpo2 function non-enzymatically, or use Xpo2 as a docking site or cofactor to mediate an effect on another component of the importin/ exportin machinery. A link between binding of SpvD to Xpo2 and the effect of SpvD on KPNA1 was established by showing that the SpvD-induced accumulation of KPNA1 at the nuclear lamina was dependent on Xpo2. Sequestration of KPNA in the nuclear envelope has been observed in cells treated with histone deacetyltransferase inhibitors [59]. However, this treatment also resulted in accumulation of Xpo2 in nuclear aggregates [59], which are clearly distinct from the effects of SpvD.
Due to the lack of clear and consistent data on the contribution of SpvD to Salmonella virulence [27][28][29][30], we reassessed its effect on systemic growth of bacteria in mice. Our evidence indicates that SpvD does contribute to systemic virulence in the mouse. The disparity might be attributable to the different mouse strains used in each study. We did not detect a significant difference in the levels of tnf-α and il1-β mRNA in splenic macrophages of wild-type mice infected with the wild-type or spvD mutant strains. However, the complemented mutant in which SpvD was produced from a plasmid caused a significant reduction of tnf-α and il1-β mRNA levels in splenic macrophages of wild-type mice. It seems likely that the effects of SpvD produced by wild-type bacteria are subtle and can be masked by other immune-modulating bacterial molecules present in these relatively short-term infection assays, as evidenced by the initial experiments using wild-type macrophages.
Together, our evidence suggests that an interaction between SpvD and Xpo2 disrupts the nuclear/cytoplasm cycling of KPNA1 and KPNA3, which interferes with nuclear translocation of p65 and activation of NF-κB regulated promoters (Fig 5E). Whatever the precise mechanism, it is remarkable that Salmonella has evolved the means to inhibit the two main pro-inflammatory signalling pathways that are activated following detection of bacterial pathogens through two proteins (SpvC and SpvD) that are encoded by adjacent genes on the same operon (Fig 5E).

Bacterial strains and plasmids
The S. Typhimurium strains (wild-type 12023 and its mutant derivatives) and plasmids used in this study are listed in S2 Table. Primers used for construction of all strains or plasmids are listed in S3 Table. Bacteria were grown in Luria Bertani (LB) medium at 37°C with shaking and supplemented with ampicillin (50 μg/ml), kanamycin (50 μg/ml) or chloramphenicol (34 μg/ml) as appropriate. S. Typhimurium mutant strains were constructed using a one-step λ Red recombinase chromosomal inactivation system [60]. Plasmid pKD4 was used as the template to amplify the kanamycin (km) resistance gene and amplification reaction products were transferred into pKD46-containing bacteria expressing λ Red recombinase by electroporation. To excise the km resistance marker, the mutant strains were transformed with pCP20 helper plasmid expressing the FLP recombinase. Virulence plasmid-encoded spvD was double hemagglutinin (HA)-tagged using the procedure described previously [61]. To obtain pACYCspvD-2HA, the double-HA tagged spvD was amplified from the virulence plasmid by PCR and cloned into pACYC184. To obtain pRK5-myc-SpvD, pRK5-myc-SpvC, pRK5-myc-YopP and pRK5-myc-NleC, the genes of interest were amplified by PCR and cloned into pRK5-myc. To obtain pRK5-myc-SpvD K185A , site-directed mutagenesis was performed by inverse PCR using pRK5-myc-SpvD as DNA template. Lysine at residue 185 of SpvD was changed to Alanine using SpvD K185A 1 and SpvD K185A 2. To create pcDNA3.1Xpo2-FLAG, the pCDNA3.1Xpo5-FLAG construct [62] was digested by NheI and XhoI restriction enzymes to obtain pcDNA3.1 vector backbone. In parallel, total cellular RNA was extracted from HeLa cells using the Qiagen RNeasy mini kit following manufacturer's instructions. RNA were reverse transcribed to cDNA using the SuperScript II Reverse Transcriptase kit (Invitrogen) and Xpo2, encoded by the gene CSE1L, was amplified with the Expand Long Template PCR system (Roche) from total cDNA using primers CAS NheI and CAS XhoI and cloned into pcDNA3.1. Obtained plasmids were verified by sequencing.

pH shift and protein secretion assay
Protein secretion assays were done as described previously [31]. Briefly, bacterial strains were grown overnight in LB broth and sub-cultured in MgM-MES medium pH 5.0 for 4 h to assemble and activate SPI-2 T3SS. Bacterial cells were then collected by centrifugation, re-suspended into MgM-MES at pH 7.2 and incubated at 37°C for 1.5 h.

Cell culture
HeLa (human epithelial cell line) cells, HEK (human embryonic kidney) cells and RAW264.7 macrophages used in this study were obtained from the European Collection of Animal and Cell Cultures (Salisbury, UK) and maintained in Dulbecco's modified Eagle's medium (DMEM) (Life technologies) supplemented with 10% foetal calf serum (FCS) (PAA Laboratories or Sigma-Aldrich) at 37°C in 5% CO 2 . Primary bone-marrow macrophages (BMM) were obtained from C57BL/6 wild-type (Charles River) or TLR4 -/mice (kind gift from Prof Maria Belvisi and Dr Mark Birrell (Respiratory Pharmacology, National Heart and Lung Institute, Imperial College, London)). BMM were grown in RPMI (Life Technologies) supplemented with 10% FCS, 2 mM glutamine, 1 mM sodium pyruvate, 10 mM HEPES, 50 μM β-mercaptoethanol, 100 U/ml penicillin/streptomycin (Sigma-Aldrich), and L929 cell-conditioned medium 20% (vol/vol; National Institute for Medical Research). After 3 days of culture, further fresh complete medium containing L929-cell conditioned medium was added to the growing macrophages. On day 7, cells were washed and seeded in complete medium without antibiotic and incubated for 24 h before bacterial challenge.

DNA transfection
HeLa cells were seeded in 24-well plates at a concentration of 2 x 10 4 cells/well 24 h before transfection with Lipofectamine 2000 (Life technologies) following the manufacturer's protocol. Unless indicated, 100 ng of DNA were used. Cells were used 24 h after transfection.

siRNA transfection
The siRNA oligo duplex targeted against Xpo2 was custom synthesized using a previously described targeting sequence [64] (ThermoScientific). The scramble control oligos (Thermo-Scientific) were designed not to target any human mRNA transcript.
HeLa cells were seeded in 24-well plates at a concentration of 5 x 10 4 cells/well and grown until they were 70% confluent. siRNA transfection using RNAiMAX (Life technologies) was carried out according to the manufacturer's protocol with a final concentration of siRNA oligos of 10 nM. A scramble oligo sequence was included in all experiments as a negative control. Cells were used 72 h after siRNA transfection.

Bacterial infection of cells
HeLa cells and RAW264.7 macrophages were infected with S. Typhimurium strains as described previously [65]. BMM were infected as described previously [66].

TNF-α stimulation
HeLa cells were seeded into 24-well plates at a concentration of 2 x 10 4 cells/well 24 h before stimulation. Unless indicated, cells were stimulated with TNF-α (10 ng/mL) for 45 min at 37°C. For IκBα degradation analysis, at indicated times after TNF-α stimulation, cells were washed and either lysed for immunoblotting analysis or trypsinised for flow cytometry assays.

Fixation, permeabilisation, fluorescence labelling and microscopy
All samples were fixed in 3% paraformaldehyde (PFA) and unless indicated, permeabilised in 0.2% Triton-X100 for 6 min. All antibodies were diluted to the appropriate concentrations in PBS containing 10% horse serum. The coverslips were washed twice in PBS, incubated with primary antibodies for 1 h, washed 3 times in PBS, incubated with secondary antibodies for 30 min and stained with the nucleic acid dye, DRAQ5 (Alexis) for 20 min. For specific plasma membrane permeabilisation, all antibodies were diluted in PBS containing 10% horse serum and 0.1% saponin (Fisher) and after fixation with PFA, cells were washed twice in PBS, incubated with primary antibodies for 1 h, washed 3 times in PBS and incubated with secondary antibodies for 30 min. Coverslips were washed and mounted onto glass slides using Mowiol mounting medium. Cells were analysed using either an epi-fluorescence microscope (BX50; Olympus) or a confocal laser-scanning microscope (LSM510 or LSM710; Zeiss GmBH). All scorings were done blindly and at least 100 cells were analysed per coverslip. To determine p65 intensity in the nucleus, confocal three-dimensional Z-stacks were acquired for each sample using a 63x objective with a slice of increment of 0.5 μm. At least 50 infected cells were imaged for each condition. Rendered three-dimensional stacks were analysed with Volocity image analysis software (Perkin Elmer).

Cell lysis, cell fractionation and immunoblotting
HeLa cells were collected from a well in a 24-well plate 3 days after transfection with siRNAs. Infected BMM were collected from a well in a 24-well plate at 10 h post-bacterial uptake. For IκBα degradation analysis, at indicated times after TNF-α stimulation, cells were washed and collected after trypsinisation. Cells were lysed in 5x sample buffer (0.25 M Tris-Cl pH 6.8, 10% SDS, 10% β-Mercaptoethanol, 10% glycerol, 0.05% Bromophenol Blue). Samples were boiled for 5 min and separated by SDS-PAGE. Samples were then transferred onto PVDF Immobilon-P membranes (Millipore), and Immunoblotting was carried out according to the manufacturer's instructions. Immunoblots shown in each figure are representative of three independent experiments.
For cell fractionation, 5 x 10 6 cells were collected, washed twice with cold PBS and lysed in cold hypotonic buffer (10 mM HEPES pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT) supplemented with complete protease inhibitor cocktail without EDTA (Roche) on ice for 15 min. NP-40 was added to a final concentration of 0.625% and cells were vortexed vigorously for 10 s. Samples were centrifuged for 30 s at 16000 g and the supernatants were harvested as cytoplasmic fraction. The nuclear pellets were then resuspended in cold hypertonic buffer (20 mM HEPES pH 7.9, 0.4 M NaCl, 1 mM EDTA, 1 mM EGTA, 1 mM DTT) supplemented with complete protease inhibitor cocktail (Roche). The samples were incubated at 4°C for 15 min with agitation. The supernatants were collected after centrifugation for 5 min at 16000 g as nuclear proteins. Cytoplasmic and nuclear proteins were frozen in 5x sample buffer at -20°C until use.

Flow cytometry quantification of cellular protein levels
For flow cytometry assays to determine protein levels by immunolabelling, trypsinised cells were washed twice in PBS prior to fixation in 3% PFA for 20 min at room temperature. Cells were then washed twice in PBS and permeabilised in 0.08% Triton X-100 for 10 min. Cells were incubated for 1 h at room temperature with primary antibodies diluted in PBS. Cells were then washed twice and incubated for 30 min with secondary antibodies diluted in PBS. Finally, cells were washed and resuspended in PBS. Flow cytometry was performed on a two-laser, four colour FACSCalibur cytometer (BD Biosciences) using Cell Quest Pro software. For each sample, 45,000-50,000 events were analysed. Collected data were analysed with FlowJo 8.1.1 software (Treestar).

RNA extraction, microarray analysis and qRT-PCR
Cells subjected to LPS (1 μg/ml) stimulation for 2 h were used as controls. At 10 h post-uptake, BMM were washed and RNA was isolated using TRIzol according to the manufacturer's directions (Invitrogen). Contaminating genomic DNA was removed using DNaseI (Qiagen). RNAs (400 ng) were reverse transcribed with Quantiscript Reverse transcriptase (QuantiTect Reverse Transcription kit, Qiagen) for 25 min at 42°C. Quantification of the mRNA levels was done using SensiMix dT kit (Quantace) and specific primers (S3 Table) on Rotor-Gene 3000 (Corbett Research). Luciferase reporter assays HEK293 cells were seeded at a density of 5 x 10 4 cells per well in a 24-well plate 16 h prior to transfection. Cells were transfected for 16 h with 50 ng of luciferase reporter plasmid (AP-1 or NF-κB dependent luciferase reporter plasmid), 30 ng of pTK-Renilla luciferase, and 100 ng of expression vectors (myc-effector or myc vector alone). For MAPK reporter assays, cells were then incubated with 25 ng/ml of PMA for 6 h and harvested in 100 μl of passive lysis buffer (Promega). For NF-κB reporter assays, cells were then incubated with 10 ng/ml of TNF-α for 8 h and harvested in 100 μl of passive lysis buffer (Promega). Luciferase activity was measured using Dual Luciferase reporter assay system (Promega) and a TD20/20 Luminometer (Turner Designs) and normalised according to Renilla luciferase intensity. The data presented are from at least three independent experiments.

Enzyme-linked immunosorbent assay
Immunoprecipitation HEK cells were seeded at a density of 2 x 10 5 cells per well in a 6-well plate 16 h prior to transfection. Cells were transfected with 1 μg of indicated DNA with Lipofectamine 2000. Three wells were used per condition. Cells were lysed 24 h post-transfection in GTPase lysis buffer (50 mM Tris Cl pH 7.4, 150 mM NaCl, 5 mM MgCl 2 , 0.5% Triton X-100) supplemented with complete protease inhibitor cocktail without EDTA (Roche) on ice for 30 min. Lysates were then centrifuged at 16000 g and the supernatants were harvested and incubated with 25 μl of anti-myc conjugated beads for 2 h at 4°C. Samples were then centrifuged at 1500 g and supernatant was removed. Pellets were washed 3 times with 50 mM Tris Cl pH 7.4. Samples were then separated by SDS-PAGE and analysed by immunoblot.
HeLa cells and RAW macrophages were grown on 150 mm dishes (Corning) seeded at a concentration of 4x10 6 cells and 8x10 6 per dish, respectively. Two dishes were used per condition. Cells were then infected with S. Typhimurium strains expressing either SpvD-2HA, SpvC-2HA or SseL-2HA for 18 h. Proteasome inhibitor MG132 (10 μg/ml) was added to the culture medium for 2 h prior to cell lysis. Cells were lysed in GTPase lysis buffer supplemented with complete protease inhibitor cocktail without EDTA (Roche) on ice for 30 min. Lysates were then centrifuged at 16000 g and the supernatants were harvested and incubated with 25 μl of anti-HA beads (Pierce) for 2 h at 4°C. Samples were then placed on a magnetic rack and supernatant was removed. Pellets were washed 3 times with 5 mM Tris Cl pH 7.4, 15 mM NaCl, 0.5 mM MgCl 2 , 0.05% Triton X-100. Proteins bound to anti-HA beads were eluted using HA-peptide (Sigma-Aldrich) and were then separated by SDS-PAGE and analysed by immunoblotting.

Mice infections
To prepare the inocula, bacteria were first grown overnight in LB broth and then subcultured at a dilution of 1:100 for a further 2 h. Cultures were diluted to a concentration of 2.5 × 10 4 cfu/ ml in physiological saline.
For competitive index (CI) measurements, bacterial cultures were mixed for intra-peritoneal inoculation (0.2 mL per mouse). Viable bacteria in inocula were quantified by dilution and plating onto LB agar plates with appropriate antibiotics to distinguish between strains. Female C57BL/6 mice (Charles River, 6-12 weeks) were sacrificed at 3 days post inoculation. The spleens were removed aseptically and homogenized in distilled water by mechanical disruption. Serial dilutions were plated on LB agar for cfu enumeration. Strains were distinguished by differential counting or replica plating on antibiotic-supplemented plates. For each mouse, the CI was calculated by dividing the output ratio (i.e. strain a versus strain b) divided by the input ratio. The log CI values were used to calculate means and for statistical analyses. A wild-type strain carrying a kanamycin resistance cassette on the chromosome (STM0857, [14]) was used to represent wild-type bacteria in the CI assay. Its CI was 0.97 ± 0.18 ( Fig 5A) when compared to wild-type bacteria, indicating that kanamycin resistance cassette does not affect virulence.
For mRNA extraction, mice were infected by intra-peritoneal inoculation with 1 × 10 4 bacteria and sacrificed 48 h post-inoculation. Spleens were harvested, homogenized and splenic CD11b(+) cells enriched using magnetic beads following manufacturer's instructions (Milteneyi Biotec). RNA of purified cells was isolated using TRIzol according to the manufacturer's directions (Invitrogen). mRNA reverse transcription and qRT-PCR were done as described above.

Ethics statement
Animals were used in accordance with UK Home Office regulations. The Imperial College Animal Welfare and Ethical Review Body (AWERB) committee approved the project licence for animal research (70/7768). The following people formed the panel: Applicant Scientist, CBS site manager / NACWO, NVS, Peer Scientist and a Lay person.

Statistical analysis
All results are reported as mean ± Standard Error of the Mean (SEM). Statistical analyses were done using two-tailed unpaired Student's t-test or ANOVA followed by Bonferonni's multiple comparison test. Differences denoted in the text as significant fall below a p-value of 0.05.
Supporting Information S1 Fig. The SPI-2 T3SS does not affect mRNA levels of all the LPS-responsive genes. Wildtype and TLR4 -/-BMMs were non-infected (NI), exposed to LPS (100 ng/ml) or infected with wild-type or ΔssaV strains for 10 h and mRNA levels of serpinB2 were analysed by qRT-PCR after reverse transcription of RNA extracted from cells. The transcript levels were normalized to the levels of rsp9, which were constant under all conditions used, and then expressed relative to those of non-infected wild-type BMMs. Results are expressed as mean ± SEM of at least 3 independent experiments. (TIF)  (ctrl) or Xpo2 depleted (Xpo2 siRNA) was analysed by quantitative confocal immunofluorescence microscopy. Results are expressed as means ± SEM of 3 independent experiments and P-values were obtained using two-tailed unpaired Student's t-test ( ÃÃÃ p < 0.005). (D) Representative immunofluorescence fields of p65 localisation using anti-p65 (red) in control cells or depleted of Xpo-2 (siRNA) after TNF-α stimulation (10 ng/ml) for 45 min. Cell nuclei were stained with DRAQ5 (green). Scale bar, 5 μm. (E) Quantification of p65 intensity in the nucleus was analysed by 3D confocal microscopy in cells with and without TNF-α treatment. Data were normalised to unstimulated cells in each condition (control and siRNA) and p65 nuclear translocation was expressed as the ratio of p65 intensity in the nucleus after stimulation compared to unstimulated cells. Results are expressed as means ± SEM of 3 independent experiments and P-values were obtained using two-tailed unpaired Student's t-test ( Ã p < 0.05).