Table 1.
Percentage of viruses neutralized with a maximum neutralization.
Fig 1.
Maximum neutralization activity of bnMAbs in a U87 target cell assay.
(A) The bnMAbs are ordered from left to right according to decreasing medians of percent maximum neutralization. Each point on the graph represents the percent at which the neutralization curve plateaued for a single virus. The percentages above the bars group bnMAb medians that have the same values. The dotted line designates 90%. The bars represent the medians and the whiskered bars represent the interquartile range. The data points are colored according to which site the bnMAb targets: high-mannose patch (magenta), CD4bs (red), V2 apex (blue), and MPER (green). (B) Percent viruses neutralized with a maximum neutralization of >95% (blue), >90% (green), and >65% (red). BnMAb groups are indicated above the figure in black and described in the text.
Fig 2.
Deviation from standard dose-response curve by bnMAbs in a U87 target cell assay.
(A) Representative experiment of PG16 neutralization of HIV pseudoviruses: MGRM-Chronic-B-017.c13, slope = 1.0 (red circle); MGRM-G-022.c02, slope = 1.2 (black square); MGRM-Chronic-B-006.c06, slope = 0.7 (light gray triangle); and MGRM-AG-011.c15, slope = 0.4 (dark gray diamond). (B) The neutralization curve slopes were determined for data from Fig 1A. BnMAbs are ordered according to decreasing medians. The bars represent the medians and the whiskered bars represent the interquartile range. The data points are colored according to which site the bnMAb targets: high-mannose patch (magenta), CD4bs (red), V2 apex (blue), and MPER (green). The dotted line denotes a slope of 0.5. (C) The neutralization curve slope value of each bnMAb for each virus on the y-axis is plotted against the corresponding MPN for each virus-bnMAb pair on the x-axis. Spearman correlation was used for statistical analysis. The Spearman’s rank correlation coefficient was calculated at 0.745 and the P value <0.0001.
Fig 3.
Maximum neutralization activity of bnMAbs in a TZM-bl target cell assay.
(A) The bnMAbs are ordered from left to right according to decreasing medians of MPN. Each point on the graph represents the percent at which the neutralization curve plateaued for a single virus. The dotted line designates 90%. The bars represent the medians and the whiskered bars represent the interquartile range. The data points are colored according to which site the bnMAb targets: high-mannose patch (magenta), CD4bs (red), V2 apex (blue), and gp41 (green). (B) Percent viruses neutralized with a MPN of >65% (red), >90% (green), and >95% (blue). (C) Each point on the graph represents the percent at which the neutralization curve plateaued for a single virus against either PGT121 (magenta), PG9 (blue) or PGV04 (red). The assay system used to measure neutralization is indicated above the data points, which are filled circles for the monogram U87 assay and unfilled squares for the CAVD TZM-bl assay.
Fig 4.
Maximum neutralization activity of bnMAbs in PBMC assays.
(A) Each point on the graph represents the percent at which the neutralization curve plateaued for a single PBMC-grown clade B virus assayed in PBMC cells. The dotted line designates 90%. (B) Each point on the graph represents the percent at which the neutralization curve plateaued for a single molecular clone virus against the bnMAbs listed above the data points. Virus clones were produced in both 293T cells and PBMCs as indicated on the x-axis. The data points are colored according to which site the bnMAb targets: high-mannose patch (magenta), CD4bs (red), V2 apex (blue), gp41-gp120 interface (green). The bars represent the medians and the whiskered bars represent the interquartile range.