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MyD88-dependent influx of monocytes and neutrophils impairs lymph node B cell responses to chikungunya virus infection via Irf5, Nos2 and Nox2

Fig 7

Mechanisms of iNOS expression in monocytes recruited to the dLN.

Mice were inoculated with PBS (mock) or 103 PFU of CHIKV AF15561 in the left footpad, and the dLN was analyzed at 24 hpi. (A) Representative flow cytometry plots and (B) percentage of CD11b+Ly6Chi monocytes expressing iNOS in WT, Ifnar1-/-, Irf3-/-Irf7-/-, or Irf3-/-Irf5-/-Irf7-/- mice. (C) Representative flow cytometry plots and (D) percentage of CD11b+Ly6Chi monocytes expressing iNOS in WT or Irf5-/- mice. (E) Representative flow cytometry plots and (F) percentage of CD11b+Ly6Chi monocytes expressing iNOS in WT, Tlr7-/-, or Myd88-/- mice. (G-H) WT mice were treated with 200 μg of IgG or α-IL-1R Ab at the time of infection. (G) Representative flow cytometry plots and (H) percentage of CD11b+Ly6Chi monocytes expressing iNOS. Errors bars represent mean ± SEM. Data are combined from 2–3 independent experiments. Statistical significance is displayed only for comparison between infected groups (mock not included) and was determined by one-way ANOVA with Tukey’s post-test (B, F) or Student’s t-test (D, H) (***, P < 0.001).

Fig 7

doi: https://doi.org/10.1371/journal.ppat.1008292.g007