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MyD88-dependent influx of monocytes and neutrophils impairs lymph node B cell responses to chikungunya virus infection via Irf5, Nos2 and Nox2

Fig 3

Monocytes or neutrophils prevent lymphocyte accumulation in the dLN during pathogenic CHIKV infection.

(A-F) C57BL/6 mice were treated with 300 μg of IgG2a isotype control Ab or anti-Ly6G mAb via intraperitoneal injection one day prior to inoculation with 103 PFU of CHIKV AF15561 in the left footpad. Representative flow cytometry plots of CD11b+Ly6Chi monocytes or CD11bhiLy6C+Ly6G+ neutrophils in the (A) blood or (B) dLN. At 5 dpi, the dLN was analyzed for (C) total cells, (D) CD19+B220+ B cells, (E) TCRβ+CD4+ T cells, and (F) TCRβ+CD8+ T cells. (G-L) C57BL/6 mice were treated with 50 μg of IgG2b isotype control mAb or anti-CCR2 mAb via intraperitoneal injection one day prior to inoculation with 103 PFU of CHIKV AF15561 in the left footpad. Representative flow cytometry plots of CD11b+Ly6Chi monocytes or CD11bhiLy6C+Ly6G+ neutrophils in the (G) blood or (H) dLN. At 5 dpi, the dLN was analyzed for (I) total cells, (J) CD19+B220+ B cells, (K) TCRβ+CD4+ T cells, and (L) TCRβ+CD8+ T cells. Errors bars represent mean ± SEM. Data are combined from two independent experiments (n = 6–9 per group). Statistical significance was determined by Student’s t-test (n.s., not significant).

Fig 3

doi: https://doi.org/10.1371/journal.ppat.1008292.g003