Skip to main content

< Back to Article

Genetic dissection of a Leishmania flagellar proteome demonstrates requirement for directional motility in sand fly infections

Fig 6

Relative fitness of Leishmania mutants in L. longipalpis infections.

The plots display abundance of barcodes at time points post bloodmeal (PBM) relative to the abundance of this barcode in the mixed parasite population used to infect L. longipalpis. Mutants are grouped according to the function of the deleted gene and severity of motility phenotype (A) flagellar mutants with severe motility defects (paralyzed, uncoordinated swimmers and aflagellate cells), (B) flagellar mutants with mild motility defects, (C) mutants lacking flagellar membrane proteins or proteins involved in protein trafficking to the flagellar membrane, (D) mutants lacking key enzymes for the synthesis of LPG and other glycoconjugates, (E) control mutants with wild type motility. Data points represent the average of three replicates. Error bars show the standard deviation of the mean of the three replicates. Dotted red lines indicate two standard deviations above and below the parental cell line (SBL1-5). Measurements were compared (two-sided t-test) to the average of all five parental controls and p-values are indicated: *≤0.05, **≤0.005, ***≤0.0005.

Fig 6