Genetic dissection of a Leishmania flagellar proteome demonstrates requirement for directional motility in sand fly infections
Categories of mutant phenotypes: among cells with normal morphology there were normal swimmers as well as slower or faster swimmers. Four categories of distinct morphological defects were observed, which all lead to impaired motility: no flagellum, short flagellum, curled flagellum and disrupted PFR. One representative mutant is shown for each category, the deleted gene is indicated. Green, PFR2::mNG signal; red, Hoechst-stained DNA. Scale bar, 2 μm.