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Genetic dissection of a Leishmania flagellar proteome demonstrates requirement for directional motility in sand fly infections

Fig 2

Enrichment plot of flagellar and cell body proteins.

Proteins detected by MS were quantified with SINQ. Plotted on the X-axis is the log2 fold change of the spectral index (CS+CI / FS+FI) and on the Y-axis the log2 fold change of the spectral index (FS+CS / FI+CI). To allow plotting of proteins that were only detected in one fraction, a value of 10−10 was inserted for missing spectral indices. The resulting diagonal lines in each quadrant represent proteins uniquely detected in the respective fraction. (A) Each data point represents one of 2414 proteins detected in MS run 1 and bubble size reflects protein abundance. Coloured circles indicate representative proteins for different flagellar sub-structures (GeneIDs in S2 Table). The plot can be interactively explored on (B) Correlation with RNA-seq data. All 2414 proteins detected in MS run 1 were plotted as in (A) and colour-coded according to the log2 fold change of differentially expressed transcripts between promastigotes (PRO) and amastigotes (AMA) [29].

Fig 2