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Exclusive dependence of IL-10Rα signalling on intestinal microbiota homeostasis and control of whipworm infection

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IL-10 signalling controls caecal immunopathology maintaining epithelial architecture during T. muris infection.

Caecal histopathology of uninfected and T. muris-infected (high dose, 400 eggs) WT, Il10-/-, Il10ra-/- and Il10rb-/- mice upon culling. (A) Representative images from sections stained with H&E. Uninfected WT and mutant mice show no signs of inflammation. Upon infection, WT mice present goblet cell hyperplasia and IL-10 signalling-deficient mice show submucosal oedema (asterisks) and large inflammatory infiltrates in the mucosa with villous hyperplasia and distortion of the epithelial architecture. T. muris worms are infecting the mucosa (arrows) of IL-10 signalling-deficient mice. Scale bar, 250μm. (B) Caecum inflammation scores and (C) goblet cells numbers per crypt were blindly calculated for each section. Data from two independent replicas (n = 5–18 each group). Mean and standard error of the mean (SEM) are shown. IDA analysis, ****p<0.0001, ***p<0.0005, **p<0.005, *p<0.05.

Fig 2