KSHV-induced ligand mediated activation of PDGF receptor-alpha drives Kaposi's sarcomagenesis
(A) PDGFA, PDGFB and GAPDH determined in 3 different samples of Normal Skin and mECK36 tumors from 3 different mice by immunoblotting. (B) Immunohistochemical staining of mouse KS-like mECK36 tumors for PDGFA, PDGFB, LANA, and phospho-PDGFRA. Representative image of the FFPEs obtained from the mECK36 tumorigenesis experiment N = 5.(C) Immunohistochemical staining of human KS biopsies for PDGFA, PDGFB, LANA, and phospho-PDGFRA. (D) IFA of mECK36 tumors for KSHV LANA (red) and phospho-PDGFRA (green). Cell nuclei were counterstained with DAPI (blue). White arrows indicate cells that co-stain for LANA and p-PDGFRA.