Advertisement

< Back to Article

Identification of virus-encoded microRNAs in divergent Papillomaviruses

Fig 8

PV-encoded miRNAs can regulate transcript sequences in early genes.

RISC reporter assays for the PV-encoded miRNAs. In all panels, the average Renilla luciferase activity normalized to firefly luciferase activity is shown, error bars indicate Standard Error, and asterisks indicate statistical significance, (*) p≤0.05; (**) p≤0.01. (A) HEK293T cells were co-transfected with either a control empty miRNA expression vector (control) or the indicated PV miRNA expression vector along with both the normalization control firefly luciferase vector and a Renilla luciferase-based reporter plasmids with vector UTR (Empty 3’UTR) or FcPV1 genomic DNA containing both putative miRNA docking sites (FcPV1 Early), N = 4. Statistical test performed was a One-Sample t Test. (B) HEK293T cells were co-transfected with either the SV40 miRNA expression vector (Control) or the indicated PV miRNA expression vector and both the control firefly luciferase vector and the Renilla luciferase-based reporter plasmids with vector UTR (Empty), FcPV1 E1 genomic sequence (E1), or the seed sequence mutant (E1 mut), N = 4. Statistical test performed was a One-Sample t Test. (C) HEK293T cells were co-transfected with either the SV40 miRNA expression vector (Control) or the indicated PV miRNA expression vector and both the control firefly luciferase vector and the Renilla luciferase-based reporter plasmids with vector UTR (Empty), FcPV1 E2 genomic sequence (E2), or the seed sequence mutant (E2 mut), N = 12. Statistical test performed was a One-Sample t Test. (D) HEK293T cells were co-transfected with either the SV40 miRNA expression vector (Control) or the HPV41 miRNA expression vector and both the control firefly luciferase vector and the Renilla luciferase-based reporter plasmids with vector UTR (Empty), HPV41 genomic DNA containing both putative miRNA sites (HPV41 Early), the site in E1 (HPV41 E1), the seed sequence mutant (E1 mut) N = 7 or the site in E2 (HPV41 E2), N = 4. Statistical test performed was a One-Sample t Test.

Fig 8

doi: https://doi.org/10.1371/journal.ppat.1007156.g008