Innate immune sensor LGP2 is cleaved by the Leader protease of foot-and-mouth disease virus
SK6 cells were transfected with a plasmid encoding (A) hLGP2-Myc-DDK or (B) DDK-poLGP2 and 24 h later infected with type-O or type-C FMDV isolates at an MOI of 5. Cells were lysed at different times after infection and analyzed by western blot using the indicated antibodies. Viral titers in the supernatants of transfected/infected cells at each time point are depicted. The N- and C-terminal cleavage products of LGP2 are indicated with arrows.