A cell-based infection assay identifies efflux pump modulators that reduce bacterial intracellular load
Salmonella were incubated with either ethidium bromide or Nile red without glucose, treated with compound, exposed to glucose, and then monitored for fluorescence. Data for each sample were normalized to the initial fluorescence (100%). Dose response curves are (A) ethidium bromide at 28 minutes after glucose addition, (B) Nile red at 7 minutes after glucose addition, and (C) Nile red after washout of compound 7 minutes after glucose addition. Mean of at least two biological replicates performed in duplicate. * p < 0.05; ** p < 0.01; *** p < 0.001; ****p < 0.0001 compared to DMSO + glucose by one-way ANOVA and Dunnett’s multiple comparison post-test.