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Species-specific vulnerability of RanBP2 shaped the evolution of SIV as it transmitted in African apes

Fig 6

SIV adapted to bind RanCyp during its transmission from monkeys to apes, but not from apes to humans.

A) The gag portion from SIVcpz was derived from SIVrcm after a cross-species transmission of a recombinant SIV into chimpanzees [21]. (But see [22] for extended discussion of this transmission event.) B) HIV-1 arose after several different zoonoses of SIV from chimpanzees and gorillas [18,23]. A, B) CRFK cell lines transduced to stably express the indicated TRIM-RanCyp (as indicated at the top of each graph) were infected with viruses encoding the indicated cyclophilin-binding loop (X-axis) in the HIV-1 backbone, and a GFP reporter. The percentage of cells infected in each sample was normalized to an empty vector control. Infections were performed in triplicate and error bars represent twice the standard error of the mean.

Fig 6