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Human cytomegalovirus UL23 inhibits transcription of interferon-γ stimulated genes and blocks antiviral interferon-γ responses by interacting with human N-myc interactor protein

Fig 3

Inhibition of Nmi interaction with STAT1 by UL23 in the absence (lanes 1–8) and presence of HCMV infection (lanes 9–16).

(A) U251 (lanes 1, 3, 5, 7) and U251-UL23 cells (lanes 2, 4, 6, 8) were treated with IFN-γ (1000 U/ml) for 12 hours and then harvested to generate protein lysates. (B) The IFN-γ treated U251 cells were infected with HCMV TowneBAC (lanes 10, 12, 14, 16) or ΔUL23 (lanes 9, 11, 13, 15) (MOI = 1) at 12 hours post-treatment and protein lysates were generated at 24–48 hours postinfection. The input protein samples (80 μg) (Input) (lanes 1, 2, 9, and 10) and samples (15 μg) that were precipitated with anti-UL23 (IP (anti-UL23)) (lanes 3, 4, 11, and 12), anti-Nmi (IP (anti-Nmi)) (lanes 5, 6, 13, and 14), or anti-STAT1 (lanes 7, 8, 15, and 16), were separated on SDS-containing polyacrylamide gels and assayed with Western blot analysis with indicated antibodies.

Fig 3

doi: https://doi.org/10.1371/journal.ppat.1006867.g003