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Prohibitin plays a critical role in Enterovirus 71 neuropathogenesis

Fig 6

Intracellular PHB co-localizes with EV71 replication complex.

(a) NSC-34 cells were infected with EV71 at M.O.I. 10 and fixed with methanol at 48 h.p.i. prior to immunostaining. Uninfected cells served as control. Scale bar represents 20μm. (b) Total cell lysate of infected NSC-34 cells was pulled down with anti-PHB or IgG isotype control antibodies prior to immunoblotting. Mock-infected cells and IgG isotype served as control. IP, immunoprecipitation; IB, immunoblot. One representative of two independent experiments is shown.

Fig 6