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Spatiotemporal dynamics of HSV genome nuclear entry and compaction state transitions using bioorthogonal chemistry and super-resolution microscopy

Fig 9

Effects of inhibition of transcription, translation and virus DNA replication on transitions in genome decompaction.

Representative images of cells infected with HSV-1EdC (moi 10) and incubated in the presence of ActD (5 μg/ml), ACV (500 μM), PAA (400 μg/ml), CHX (100 μg/ml), or no treatment. Infection was synchronised as described in Fig 5 and cells fixed at the time points indicated for processing (scale bar 10 μm). Arrows and circles indicate qualitative features of genome localisation under each condition as discussed in the text.

Fig 9

doi: https://doi.org/10.1371/journal.ppat.1006721.g009