Advertisement

< Back to Article

HBV core protein allosteric modulators differentially alter cccDNA biosynthesis from de novo infection and intracellular amplification pathways

Fig 6

HBV capsid assembly modulator treatment confers HBV virion DNA sensitivity to DNase digestion.

(A) Each of the endogenous DNA polymerase reaction contains approximately 108 HBV virions prepared from chronic HBV carriers and incubated in the presence or absence of dNTP and indicated concentrations of Bay 41–4109, ENAN-34017 or GLS4 at 37°C for 16 h. Viral DNA were extracted without or with prior DNase I digestion at 37°C for 30 min and detected by Southern blot hybridization with full-length riboprobe recognizing minus strand DNA. (B) Approximately 108 HBV virions in each endogenous DNA polymerase reaction were incubated in the absence of dNTP (except for lanes 2, 3, 19 and 20) and absence or presence of the indicated concentrations of Bay 41–4109, ENAN-34017 or GLS4 at 37°C for 16 h. Viral DNA were extracted and analyzed as described above.

Fig 6

doi: https://doi.org/10.1371/journal.ppat.1006658.g006